LOCUS       mu6-mClover-gRNA-Sa-mSc 8625 bp ds-DNA     circular     20-FEB-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          703..725
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 4224-4246 FWD 3"
                     /note="sequence: acagcacaaaaggaaactcaccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          706..726
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 4227-4247 FWD 2"
                     /note="sequence: gcacaaaaggaaactcaccct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     794..813
                     /label="Amp-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(923..944)
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 4444-4465 REV 3"
                     /note="sequence: gtaatacggttatccacgcggc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(923..944)
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 4444-4465 REV 2"
                     /note="sequence: gtaatacggttatccacgcggc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(1032..1136)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(1220..1239)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     enhancer        1411..1790
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1452..1473
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 6722-6743 FWD 1"
                     /note="sequence: gtaacaactccgccccattgac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1536..1555)
                     /label="pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP REV"
                     /note="sequence: gcctcgcccttgctcaccatggtgggctagcggatctgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1556..1575
                     /label="pmScarlet_Giantin_C1 FWD"
                     /note="sequence: gtcagatccgctagcccaccatggtgagcaagggcgaggc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1745..1766)
                     /label="(sg Sa) pSLQ2853-3 pHR: U6-Sasgv2Mock CMV-mScarlet 7015-7036 REV 1"
                     /note="sequence: tggagccgtacatgaactgagg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1792..1990
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     LTR             2008..2188
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2231..2251)
                     /label="pmScarlet_Giantin_C1 REV"
                     /note="sequence: ttaggtccctcgacgaattccttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2235..2360
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2252..2274
                     /label="pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP FWD"
                     /note="sequence: gcatggacgagctgtacaaggaattcgtcgagggacctaataa"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2859..3092
                     /label="RRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3619..3736
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3795..4104
                     /label="modified mU6"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3867..3886
                     /label="sg.BsmBI.T1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3910..3935
                     /label="colonyPCR_m6_F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    3989..4022
                     /label="loxP"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     4026..4046
                     /label="mU6-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4028..4048
                     /label="pMCB320_pre-i7_fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4075..4096
                     /label="m6_F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4083..4104)
                     /label="sg.mClov.Sau6.v2R"
                     /note="sequence: aaggtggttctccaagggatac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4100..4103
                     /label="ov"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4105..4126
                     /label="sg.mClov.sa.v2F"
                     /note="sequence: gactataagtatcccttggagaaccaccttGAGACGGAAAGTGAAACGTGAT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4111..5125
                     /label="pRC0105 template from Robert"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4166..4185
                     /label="sg.BsmBI.T2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     RBS             4259..4270
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4277..4993
                     /label="mClover3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(4318..4335)
                     /label="sg.BsmBI.T2R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4499..4517
                     /label="sg.BsmBI.T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4868..4887
                     /label="sg.BsmBI.T4"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      5005..5076
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      5092..5119
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          5107..5127
                     /label="sg.mClov.SaScaff.v2F"
                     /note="sequence: GGGCCTTTCTGCGTTTATACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(5110..5132)
                     /label="sg.mClov.Sa.v2R"
                     /note="sequence: gGAGACGTATAAACGCAGAAAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5133..5153
                     /label="SaSF_linkerv1_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_RNA        5133..5208
                     /label="Sa gRNA scaffold (modified)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(5166..5188)
                     /label="Sa_gRNA_v3R"
                     /note="sequence: gataaacacggcattttgccttg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(5175..5196)
                     /label="Sa_gRNA_v2R"
                     /note="sequence: gttgacgagataaacacggcat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(5188..5208)
                     /label="Sa_gRNA_R"
                     /note="sequence: tctcgccaacaagttgacgag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(5255..5274)
                     /label="TG_SaSp_R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5273..5332
                     /label="part of CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     enhancer        5333..5636
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        5637..5840
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     regulatory      5874..5877
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    5874..5894
                     /label="mScarlet F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             5874..6569
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          5874..6569
                     /label="source"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             5878..6573
                     /label="Translation 5878-6573"
                     /translation="*ARARQ*SRSSCGSRCTWRAP*TATSSRSRARARAAPTRAPRPPS*R*PRVAPCPSPGTSCPLSSCTAPGPSPSTPPTSPTTISSPSPRASSGSA**TSRTAAP*P*PRTPPWRTAP*STR*SSAAPTSLLTAP*CRRRQWAGKHPPSGCTPRTAC*RATLRWPCA*RTAAATWRTSRPPTRPRSPCRCPAPTTSTASWTSPPTTRTTPWWNSTNAPRAATPPAAWTSCTRN"
     primer_bind     complement(6007..6025)
                     /label="mCherry-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6060..6079
                     /label="T2_scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(6106..6125)
                     /label="T1_scar"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(6191..6208)
                     /label="sg.BsmBI.Scar.T2R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     6275..6294
                     /label="mCherry-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(6514..6531)
                     /label="sg.BsmBI.Scar.T1R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(6550..6569)
                     /label="mScarlet R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     source          6570..1164
                     /label="source"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    6589..6622
                     /label="loxP"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6678..7266
                     /label="WPRE"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(6731..6751)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(6746..6765)
                     /label="sg.T.R.WPRE"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(7149..7160)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(7269..7285)
                     /label="KS primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(7270..7286)
                     /label="pBluescriptKS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     LTR             7795..7975
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(8037..8625)
                     /label="ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     complement(8117..8136)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgcttaa gcggtcgacg gatcgggaga tctcccgatc
     1201 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     1261 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     1321 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     1381 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     1441 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1501 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1561 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1621 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1681 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1741 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1801 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1861 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1921 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1981 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2041 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2101 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2161 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2221 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2281 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2341 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2401 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2461 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2521 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2581 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2641 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2701 ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     2761 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     2821 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     2881 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     2941 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     3001 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     3061 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     3121 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     3181 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     3241 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     3301 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     3361 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     3421 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     3481 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     3541 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     3601 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3661 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3721 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     3781 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     3841 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     3901 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     3961 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     4021 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     4081 aagtatccct tggagaacca ccttGAGACG GAAAGTGAAA CGTGATTTCA TGCGTCATTT
     4141 TGAACATTTT GTAAATCTTA TTTAATAATG TGTGCGGCAA TTCACATTTA ATTTATGAAT
     4201 GTTTTCTTAA CATCGCGGCA ACTCAAGAAA CGGCAGGTTC GGATCTTAGC TACTAGAGAA
     4261 AGAGGAGAAA TACTAGatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4321 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga
     4381 gggcgatgcc accatcggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4441 cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct tcagccgcta
     4501 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4561 ggagcgcacc atctacttca aggacgacgg tacctacaag acccgcgccg aggtgaagtt
     4621 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4681 caacatcctg gggcacaagc tggagtacaa cttcaacagc cactacgtct atatcacggc
     4741 cgacaagcag aacaacagca tcaaggctaa cttcaccatc cgccacaacg ttgaggacgg
     4801 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4861 gctgcccgac aaccactacc tgagccatca gtccgccctg agcaaagacc ccaacgagaa
     4921 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggattacac atggcatgga
     4981 cgagctgtac aagtaaCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC
     5041 TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC TGGCTCACCT
     5101 TCGGGTGGGC CTTTCTGCGT TTATACGTCT Ccgttatagt actctggaaa cagaatctac
     5161 tataacaagg caaaatgccg tgtttatctc gtcaacttgt tggcgagatt tttttctcga
     5221 gtactaggat ccattaggcg gccgcgtgga taaccgtatt accgccatgc attagttatt
     5281 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     5341 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     5401 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     5461 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     5521 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     5581 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     5641 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     5701 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     5761 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     5821 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagccc accatggtga
     5881 gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca
     5941 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6001 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt
     6061 cccctcagtt catgtacggc tccagggcct tcaccaagca ccccgccgac atccccgact
     6121 actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6181 gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg
     6241 tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg
     6301 gctgggaagc atccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta
     6361 agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca
     6421 aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca
     6481 cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact
     6541 ccaccggcgg catggacgag ctgtacaagg aattcgtcga gggacctaat aacttcgtat
     6601 agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg
     6661 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6721 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6781 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6841 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6901 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6961 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7021 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7081 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7141 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7201 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7261 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     7321 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7381 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7441 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7501 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7561 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     7621 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     7681 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     7741 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg
     7981 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8221 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8341 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8401 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8461 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8521 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8581 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//