LOCUS       mu6-mClover-gRNA-Sp-GFP 8655 bp ds-DNA     circular     20-FEB-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     794..813
                     /label="Amp-R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        complement(1032..1136)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(1220..1239)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        1411..1790
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        1792..1990
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     LTR             2008..2188
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2235..2360
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2859..3092
                     /label="RRE"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3619..3736
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3795..4105
                     /label="modified mU6"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3910..3935
                     /label="colonyPCR_m6_F"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    3989..4022
                     /label="loxP"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     4026..4046
                     /label="mU6-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4028..4048
                     /label="pMCB320_pre-i7_fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4075..4096
                     /label="m6 F (NP)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4085..4105)
                     /label="sg.mClov.Spu6.v2R"
                     /note="sequence: caaggtggttctccaagggat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4101..4104
                     /label="ov"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4106..4127
                     /label="sg.mClov.sp.v2F"
                     /note="sequence: actataagtatcccttggagaaccaccttgGAGACGGAAAGTGAAACGTGAT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4112..5126
                     /label="pRC0105 template from Robert"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     RBS             4260..4271
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4278..4994
                     /label="mClover3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      5006..5077
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      5093..5120
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(5111..5133)
                     /label="sg.mClov.Sp.v2R"
                     /note="sequence: aacttgctatgctgtttccagcatagctctgGAGACGTATAAACGCAGAAAGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5134..5137
                     /label="ov"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5134..5149
                     /label="SpSF_linkerv1_F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          5134..5155
                     /label="sg.mClov.SpScaff.v2F"
                     /note="sequence: agagctatgctggaaacagcat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5134..5221
                     /label="Sp scaffold"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(5169..5191)
                     /label="Sp_gRNA_v2R"
                     /note="sequence: caagttgataacggactagcctt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(5185..5205)
                     /label="Sp_gRNA_v3R"
                     /note="sequence: cggtgccactttttcaagttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5191..5210
                     /label="pMCB320_pre-i5_rev"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5193..5210)
                     /label="Sp_gRNA_R"
                     /note="sequence: cgactcggtgccactttt"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(5234..5252)
                     /label="SpSF_out_R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(5247..5265)
                     /label="pre-i5"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(5261..5280)
                     /label="TG_SaSp_R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     enhancer        5339..5642
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        5643..5846
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             5880..6599
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             5880..6599
                     /label="Translation 5880-6599"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     primer_bind     complement(5925..5946)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6186..6205)
                     /label="EXFP-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(6377..6396)
                     /label="sg.BsmBI.GFP.T1R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6533..6554
                     /label="EGFP-C"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    6619..6652
                     /label="loxP"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6708..7296
                     /label="WPRE"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(6761..6781)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7179..7190)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(7299..7315)
                     /label="KS primer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(7300..7316)
                     /label="pBluescriptKS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     LTR             7825..8005
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(8067..8655)
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(8147..8166)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgcttaa gcggtcgacg gatcgggaga tctcccgatc
     1201 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     1261 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     1321 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     1381 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     1441 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1501 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1561 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1621 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1681 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1741 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1801 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1861 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1921 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1981 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2041 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2101 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2161 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2221 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2281 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2341 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2401 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2461 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2521 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2581 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2641 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2701 ccggccgcgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     2761 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     2821 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     2881 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     2941 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     3001 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     3061 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     3121 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     3181 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     3241 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     3301 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     3361 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     3421 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     3481 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     3541 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     3601 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3661 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3721 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     3781 gggcccgctc tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga
     3841 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
     3901 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
     3961 acattttaca tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt
     4021 ataaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
     4081 aagtatccct tggagaacca ccttgGAGAC GGAAAGTGAA ACGTGATTTC ATGCGTCATT
     4141 TTGAACATTT TGTAAATCTT ATTTAATAAT GTGTGCGGCA ATTCACATTT AATTTATGAA
     4201 TGTTTTCTTA ACATCGCGGC AACTCAAGAA ACGGCAGGTT CGGATCTTAG CTACTAGAGA
     4261 AAGAGGAGAA ATACTAGatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4321 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     4381 agggcgatgc caccatcggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4441 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc ttcagccgct
     4501 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4561 aggagcgcac catctacttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     4621 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4681 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccactacgtc tatatcacgg
     4741 ccgacaagca gaacaacagc atcaaggcta acttcaccat ccgccacaac gttgaggacg
     4801 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4861 tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac cccaacgaga
     4921 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggattaca catggcatgg
     4981 acgagctgta caagtaaCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC
     5041 CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC
     5101 TTCGGGTGGG CCTTTCTGCG TTTATACGTC TCcagagcta tgctggaaac agcatagcaa
     5161 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     5221 tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg ccatgcatta
     5281 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     5341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     5401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     5461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     5521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     5581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     5641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     5701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     5761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     5821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcccacca
     5881 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5941 gcgacgtaaa cggccacaag ttcagcgtgt ctggcgaggg cgagggcgat gccacctacg
     6001 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6061 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     6121 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6181 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6241 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6301 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     6361 gcatcaaggc gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     6421 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6481 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     6541 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     6601 aattcgtcga gggacctaat aacttcgtat agcatacatt atacgaagtt atacatgttt
     6661 aagggttccg gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg
     6721 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6781 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6841 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6901 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6961 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7021 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7081 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     7141 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7201 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7261 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga
     7321 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7381 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7441 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7501 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7561 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     7621 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     7681 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     7741 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     7801 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     7861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7981 tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac
     8041 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8101 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8161 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8221 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8281 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8341 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8401 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8461 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8521 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8581 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8641 aaaaaaggat ctcaa
//