LOCUS       GR481_-_pUC-FDP-LEU2int 6220 bp ds-DNA     circular     13-NOV-2020
DEFINITION  .
SOURCE      Cloning vector pUC19                                               
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;        
            vectors.
FEATURES             Location/Qualifiers
     -35_signal      complement(411..416)
                     /label="bla promoter (counter-clockwise); TTCAAA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4424..4424)
                     /label="T -> C"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4421..4421)
                     /label="A -> G"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    606..608
                     /label="WT: GCT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     -10_signal      5375..5380
                     /label="RNAI promoter (clockwise); GCTACA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    607..800
                     /label="LEU2 landing pad p1 int US flank 4"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4498..4652
                     /label="Recombination flank from ORF4 pGKL1"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     rep_origin      complement(4947..5535)
                     /label="pMB1 origin of replication (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(5709..6220,1..148))
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1746..1748
                     /label="AAA -> AAC mutation to catalytic lysine"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     5'UTR           complement(1341..1440)
                     /label="UTR\from\2ORF1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     sig_peptide     complement(278..346)
                     /label="bla sig_peptide"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(join(5706..6220,1..346))
                     /label="bla gene"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3246..3248
                     /label="AAG -> TAG K384* transversion requiring mutation"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1441..1469
                     /label="UTR region from pGKL1ORF2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(4929..5611)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    801..1340
                     /label="Not present in landing pad"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_RNA        complement(4947..5499)
                     /label="RNAII transcript (complementary strand)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     mutation        complement(1336..1337)
                     /label="F->H\mutation"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2274..4397)
                     /label="trp5 K384*"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     -35_signal      5353..5358
                     /label="RNAI promoter (clockwise); TTGAAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     -10_signal      complement(5509..5514)
                     /label="RNAII promoter (counter-clockwise); CGTAAT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    549..549
                     /label="G in source"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     -10_signal      complement(390..395)
                     /label="bla promoter (counter-clockwise); GAGACA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_RNA        5389..5496
                     /label="RNAI transcript"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(join(5706..6220,1..346))
                     /label="bla CDS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(606..1340)
                     /label="LEU2 N term"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     mutation        1473..1475
                     /label="Mutation"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1470..2273
                     /label="ura3 K93N"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     -35_signal      complement(5530..5535)
                     /label="RNAII promoter (counter-clockwise); TTGAGA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4398..4497)
                     /label="10B2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4602..4652)
                     /label="7394 - GR-p1ORF4-rvs"
                     /note="sequence: actatctattttttcagtacataatttaattaaatcatcatgacctttatc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1442..1494
                     /label="5135"
                     /note="sequence: atatgaaagtttttataataattataaaATGCATAAAGCTACATATAAGGAAC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          4403..4431
                     /label="8238 - GR-10B2mid-rvs"
                     /note="sequence: atgtctatgagcttatcaCatGtttctac"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5755..5779)
                     /label="6924"
                     /note="sequence: GGAGTCAGGCAACTATGGATGAACG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4575..4598)
                     /label="6851"
                     /note="sequence: TTTTACATCTCTTCTACCAAGACC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1319..1378)
                     /label="7395 - GR-LEU2-fwd_GO:p2ORF1"
                     /note="sequence: aatgattcttaatatgatttaatagtttatgattataaatgcacgttcctaagaagatcg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4407..4447
                     /label="5846"
                     /note="sequence: ctatgagcttatcacatgtttctacatttttctgatatttg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          380..401
                     /label="3172"
                     /note="sequence: TCAGGGTTATTGTCTCATGAGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          4618..4677
                     /label="7402 - GR-pUC19-fwd_GO:p1ORF4"
                     /note="sequence: atttaattaaattatgtactgaaaaaatagatagtactATTAATTGCGTTGCGCTCACTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1824..1843)
                     /label="72 - seq_US URA"
                     /note="sequence: ACCACACCGTGTGCATTCGT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3224..3247)
                     /label="B595 - GR-TRP5-I385-fwd"
                     /note="sequence: AGATCAACAATGCTTTAGCACAAG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          6047..6072
                     /label="7050"
                     /note="sequence: cgtttggtatggcttcattcagctcc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          6147..6169
                     /label="6292"
                     /note="sequence: GATCGTTGTCAGAAGTAAGTTGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1414..1473
                     /label="B998 - GR-p1O2-URA3-fwd_GO:p2O1"
                     /note="sequence: tatgacctaattgactccggcgaaaaatatatgaaagtttttataataattataaaatgc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2274..2295
                     /label="6643"
                     /note="sequence: TTAGGCAGATGGGTCTTCTTCG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(4739..4759)
                     /label="8053"
                     /note="sequence: CAATACGCAAACCGCCTCTCC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          573..632
                     /label="A220 - GR-LEU2mid4-rvs_GO:pUC19backbone"
                     /note="sequence: AACCTCTGACACATGCAGCTCCCGGAGACGagtActggtgattataataccatttaggtg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(1419..1440)
                     /label="7397 - GR-p2ORF1-fwd"
                     /note="sequence: tttttcgccggagtcaattagg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3218..3269
                     /label="B594 - GR-trp5-rvs_GO:K384*"
                     /note="sequence: GAAGAACTTGTGCTAAAGCATTGTTGATCTaGTGAGATCCCGTGTGGTTAAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          1919..1938
                     /label="390"
                     /note="sequence: AGCAGAATTGTCATGCAAGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2257..2305)
                     /label="B593 - GR-URA3-rvs_GO:TRP5"
                     /note="sequence: ATTTGAGATTCGAAGAAGACCCATCTGCCTAAttagttttgctggccgc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4498..4520
                     /label="5257"
                     /note="sequence: cctcagactcagattggtgtttc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3291..3311
                     /label="6214"
                     /note="sequence: TTGAGCACCTTGACAATGCTC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(764..784)
                     /label="5467"
                     /note="sequence: CTATTTGGTCCTTGGATAAAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4375..4427)
                     /label="B596 - GR-TRP5-fwd_GO:10B2"
                     /note="sequence: aaaCatGtgataagctcatagacatgtaaaATGTCAGAACAACTCAGACAAAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          442..460
                     /label="3467"
                     /note="sequence: GGGTTCCGCGCACATTTCC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1760..1781
                     /label="9458"
                     /note="sequence: TGGTAATACAGTCAAATTGCAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1341..1384
                     /label="7396 - GR-p2ORF1-rvs"
                     /note="sequence: ttataatcataaactattaaatcatattaagaatcattcaattg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2616..2635
                     /label="6216"
                     /note="sequence: ATCATGGACTTGACCATCAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4398..4424
                     /label="7400 - GR-10m2-rvs"
                     /note="sequence: tttacatgtctatgagcttatcaCatG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1319..1338
                     /label="A686"
                     /note="sequence: cgatcttcttaggaacgtgc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5324..5341
                     /label="1264"
                     /note="sequence: CGAGGTATGTAGGCGGTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(5336..5355)
                     /label="5484"
                     /note="sequence: CAAGAACTCTGTAGCACCGC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2021..2040)
                     /label="391"
                     /note="sequence: TTCCACCCATGTCTCTTTGA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6147..6169)
                     /label="6293"
                     /note="sequence: CCAACTTACTTCTGACAACGATC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6036..6066
                     /label="6043"
                     /note="sequence: gtcacgctcgTCGTTTGGTATGGCTTCATTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2217..2238
                     /label="2742 - seq_DS URA"
                     /note="sequence: CGTTACAGAAAAGCAGGCTGGG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(591..608)
                     /label="A218 - GR-pUC19-backbone-rvs"
                     /note="sequence: agTactCGTCTCCGGGAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          469..491
                     /label="8048"
                     /note="sequence: TGCCACCTGACGTCTAAGAAACC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
       61 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
      121 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
      181 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
      241 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
      301 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
      361 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
      421 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG
      481 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
      541 TTCGTCTCaC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
      601 CGagtActgg tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg
      661 gcagaatcaa tcaattgatg ttgaaccttc aatgtaggga attcgttctt gatggtttcc
      721 tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa
      781 ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc
      841 acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt
      901 ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta
      961 gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt
     1021 cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta
     1081 acactaccgg taccccattt aggaccaccc acagcaccta acaaaacggc atcagccttc
     1141 ttggaggctt ccagcgcctc atctggaagt ggaacacctg tagcatcgat agcagcacca
     1201 ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta
     1261 agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg
     1321 atcttcttag gaacgtgcat ttataatcat aaactattaa atcatattaa gaatcattca
     1381 attgattttt gggatttgga ttatatagaa aagtatgacc taattgactc cggcgaaaaa
     1441 tatatgaaag tttttataat aattataaaa tgcataaagc tacatataag gaacgtgctg
     1501 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     1561 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     1621 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     1681 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     1741 acagaaaCtt tgctgacatt ggtaatacag tcaaattgca gtattctgcg ggtgtataca
     1801 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     1861 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     1921 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     1981 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     2041 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     2101 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     2161 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     2221 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaTTAGGCA
     2281 GATGGGTCTT CTTCGAATCT CAAATCCCAA CCTATCTTTG GACCTAATTT CGGCAAGACT
     2341 TCAGCGACAC TTTGGACATC TTTATCACCT CTACCAGAAA TATTGATAAC CAAATGTTGA
     2401 TCAGGCTTCA TCGTCTTAGC CAATTCGCAA GCGCCATAAA CAGCATGAGA AGATTCCAAA
     2461 GCGGGAATAA TACCTTCTAA TTGAGATAAT AATTTAAAGC CAAGCAGAGC CTGAGCGTCA
     2521 GTAGCTGCAA TGAATTGAGC ACGGCCAGTA GATTTCCAAT ATGCCAATTC TGGACCGACA
     2581 CCTGGGTAGT CTAACCCAGC AGAAACAGAA TGAGTATCAT GGACTTGACC ATCACTATCT
     2641 TGCAAGACAT AAGTCTTGAC ACCATGGAAG ACACCAGGTC TACCGGCAGT TAGAGTAGCA
     2701 GAGTGGAACT TTGTATCTAC ACCATCACCA CCGGCTTCCA CACCCAATAA CTTAACGGAA
     2761 GTATCATGCT CGAATGGTGA AAACATACCT GTAGAGTTGG AACCACCCCC AACACATGCA
     2821 ACAACTGCGT CAGGTAATTT ACCATTGTTC ATGGCAGCAA ACTGTTCCTT GGTTTCTTTA
     2881 CCAATGACAC TTTGGAAAGT TCTAACCAAA GTTGGATATG GGTGAGGACC AATGGCAGAA
     2941 CCGACGACGT AGTAAGTAGT TTTCAAGTTA GTAACCCAAA ATCTGAATGC CTCTGAAGTA
     3001 GCGTCTCTTA GAGTCTTTGT ACCATTAGTA ACAGCAATTA CTTTAGCACC GAGAATTCTC
     3061 ATTCTGAAGA CGTTTAAAGC TTGGCGACGA ACATCTTCTG CACCCATGAA CACAGTACAG
     3121 GTTAAGCCAA ATTTAGCACA TGCAGTGGCA GTGGCAACAC CGTGTTGACC AGCACCGGTT
     3181 TCAGCAATAA CGTTCTTCTT GCCTAATCTT TTAGCTAGAA GAACTTGTGC TAAAGCATTG
     3241 TTGATCTAGT GAGATCCCGT GTGGTTAAGA TCTTCTCTCT TCAACCAGAT TTGAGCACCT
     3301 TGACAATGCT CAGTTAATCT CTCAGCTTTG TGTAGTGAAG AAGGACGGCC AATATAAGAA
     3361 TACAAGGATT TGAAGTCTTC CCAGAATGTG GGATCGGCGA CAGCTTCATC AAAACCCTTT
     3421 TCCAACTCTC TTAGACATGC ATGAAGAGCT TCTGGGACAT ACTGACCACC AAAGTCCCCA
     3481 AATCTAATTG GGTGCTTGTG ATTTTCATCA AATTCGTCCA GTATTTCCTT CTTAACGTTT
     3541 GCGGACTTCA AGGACTCTTT TTGAAAGGCA AAGAATTCGT CCTTGGACAG AACCTTATGC
     3601 TTAGCACCAT TTAGAATTCC CTGTACATAT TCCTTAGCAA CGTCGTACCT TTTGCCCTCT
     3661 GGAGCATCTC CACATAATGT GACGATTTTG GAACCAATCA CTACACCGTC AGCAACACTA
     3721 CCAACTGATT GGAAATGTTC TCTGGTAGAG ACACCAAACC CAACGGCCAA AGGAGTATCC
     3781 TTGGTGTACT TTCTGACTCT AGAGATGAGT TCATCCAAAT CACTGGCCAC AGAACTTTGA
     3841 ACACCAGTAG TACCCATTCT AGACACAACG TAGACAAACG AATCGGCAAT ATGCGATAGT
     3901 AATTCCAATC TTTCGTCGGT GGTAGAAGGA GCCACTAGTG GGATCAGGCT CAAACCATTA
     3961 TCATTGATGT AGTTTCTGAC CTTCAACGCC TCCTCTGGTG GCAAATCGAC GATGATAAAA
     4021 CCATTAGCAC CAGCCTTGGC AGCGTCCTGA ATAAATCTTT CTTCACCGTA GTTTAGAATA
     4081 GGGTTATAGT AACCCATTAG GATTATGGGT ACGGTAACAC CTTCATTTCT AGCTTGGGAG
     4141 ACCATTTCTA GAGTTTGAGG CAAGGTAACA CCGTTTTGCA AAGCCACAGT ATTAGATAAT
     4201 TGAATTGTAG GACCATCTGC AATTGGATCA GAGAAGGGCA TACCCAATTC GATGATATCT
     4261 ACACCACCAT CCTGGAAACC CTTGAGAATA GGGACAGTGT CTTTGACTGT TGGGTAACCT
     4321 GCGGTCATAA ATGTGACCAA GGCGTTCCTG TTTTCTTTTT TAGCGTTAGC AAATGTTTGT
     4381 CTGAGTTGTT CTGACATttt acatgtctat gagcttatca CatGtttcta catttttctg
     4441 atatttgaaa gttattaatc tttttgtttc tatagatctt cctatgtata ggtcatccct
     4501 cagactcaga ttggtgtttc aaagatcctc aaggcaatag aataacagat tttgatagta
     4561 ttaataaaga attaggtctt ggtagaagag atgtaaaatt agataaaggt catgatgatt
     4621 taattaaatt atgtactgaa aaaatagata gtactATTAA TTGCGTTGCG CTCACTGCCC
     4681 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     4741 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     4801 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     4861 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     4921 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     4981 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     5041 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     5101 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     5161 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     5221 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     5281 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     5341 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     5401 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     5461 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     5521 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     5581 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     5641 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     5701 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     5761 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     5821 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     5881 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     5941 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     6001 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     6061 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     6121 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     6181 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
//