LOCUS       GR475_-_pCAN1int-CAN1- 11561 bp ds-DNA     circular     17-DEC-2020
DEFINITION  .
KEYWORDS    "creator:Ria Deshpande" "marker:AmpR"                              
FEATURES             Location/Qualifiers
     protein_bind    9842..9859
                     /label="I-SceI Recognition Site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        8113..8812
                     /label="ScRPL18B Promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(793..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    10425..10441
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    10360..10365
                     /label="EcoRI site"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1245..1744
                     /label="CAN1 upsteam flank"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(10401..10417)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     rep_origin      complement(10803..11391)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(10456..10473)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1745..2444
                     /label="ScPSP2 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    10494..10515
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(10474..10479)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4443..7406
                     /label="+++ recoded wt DNAP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4689..4691
                     /label="recoded from original to abolish EcoRI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(1..792)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4218..4442
                     /label="ScENO2 Terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    897..997
                     /label="Sequenced on 6/25"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    9860..10359
                     /label="CAN1 downstream flanks"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3120..3122
                     /label="recode to abolish EcoRI site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2445..4217
                     /label="Can1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7413..8112
                     /label="REV1p"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             8813..9841
                     /label="HPH (Hygromycin Phosphotransferase)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1239..1244
                     /label="EcoRIsite"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(10449..10455)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2245..2272
                     /label="B023"
                     /note="sequence: ttcaattaggtctaatttagggttgagc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1713..1744)
                     /label="8904 - GR-CAN1.US-rvs"
                     /note="sequence: TGCTATGCCTTTTTTTTTTTTTGTTTTTACAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8090..8112)
                     /label="B028"
                     /note="sequence: gtgttgttatccgatacaaccgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          8779..8833
                     /label="B967 - GR-HPH-fwd_GO:RPL18Bp"
                     /note="sequence: agaaaaaatcaattagaagaaaacaaaaaacaaaatgggtaaaaagcctgaactc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(10036..10060)
                     /label="2741"
                     /note="sequence: CTGAAGGAGTTTCAAATGCTTCTAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(9824..9875)
                     /label="B966 - GR-HPH-rvs_GO:I-SceI-cutSite-CAN15flank"
                     /note="sequence: TAATAAAACGTCATATattaccctgttatccctattattcctttgccctcgg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5027..5048
                     /label="7506"
                     /note="sequence: CGAAGTTAACCCAtTTTTGAGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(10433..10456)
                     /label="8839"
                     /note="sequence: GTATGTTGTGTGGAATTGTGAGCG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4413..4472)
                     /label="B781 - GR-ENO2t-rvs_GO:TPDNAPv3"
                     /note="sequence: ATTGAAAAcAAaACTGTTAAgGCAACATAAatttttcaaactgcaaattcaagaaaaagc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          7230..7257
                     /label="9964"
                     /note="sequence: atattctgcgtacttcttagcatcttcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          9842..9867
                     /label="B965 - GR-I-SceI-CAN15flank-fwd"
                     /note="sequence: tagggataacagggtaatATATGACG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(6676..6706)
                     /label="9609"
                     /note="sequence: AATTGTTTACTGCAAATAAGAATAATGTTTC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          8081..8140
                     /label="B775 - GR-RPL18Bp-fwd_GO:REV1p"
                     /note="sequence: gaaaaatatccggttgtatcggataacaacacaagaggatgtccaatattttttttaagg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          9842..9894
                     /label="B782 - GR-DS.CAN1-fwd_GO:I-SceIsite"
                     /note="sequence: tagggataacagggtaatATATGACGTTTTATTACCTTTGATCACATTTCCAC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2920..2940
                     /label="1924"
                     /note="sequence: GATTCCTTTCTCCAGCATTTG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4673..4706
                     /label="A718"
                     /note="sequence: CACACAAtAAcTTATCAAATTCACATTCcAATTC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(6120..6143)
                     /label="7505"
                     /note="sequence: CATaatGGTGGTGGTTACGATTTC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4218..4252
                     /label="7272 - GR-tENO2-fwd"
                     /note="sequence: agtgcttttaactaagaattattagtcttttctgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(6365..6385)
                     /label="7012"
                     /note="sequence: CATTGTCTAAATTTTCAGATG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          4951..4974
                     /label="A738"
                     /note="sequence: ATgaaATCAGTATCCCAGATCTTg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          50..74
                     /label="6923"
                     /note="sequence: CGTTCATCCATAGTTGCCTGACTCC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1245..1276
                     /label="8903 - GR-CAN1.US-fwd"
                     /note="sequence: TATATCTTTAACAGATTCCAAACCCTAAAGTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4673..4706)
                     /label="A717"
                     /note="sequence: GAATTgGAATGTGAATTTGATAAgTTaTTGTGTG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          8113..8140
                     /label="6382"
                     /note="sequence: aagaggatgtccaatattttttttaagg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(7380..7406)
                     /label="A416"
                     /note="sequence: ATGGATTAcAAGGAcAAgGCATTGAAC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2410..2467
                     /label="B784 - GR-CAN1-fwd_GO:PSP2p"
                     /note="sequence: gatcttaatcacatagagtgctggaacgggaagaaATGACAAATTCAAAAGAAGACGC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1647..1671
                     /label="A266"
                     /note="sequence: TGGATCCAGTTTTTAATCTGTCGTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1220..1279)
                     /label="8906 - GR-intBackbone-rvs_GO:CAN1.US"
                     /note="sequence: GGACACTTTAGGGTTTGGAATCTGTTAAAGATATAgaattcactcacCAACGCGTATATC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          6516..6546
                     /label="9965"
                     /note="sequence: agagtataatctgatatcattaaaagattta"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(50..74)
                     /label="6924"
                     /note="sequence: GGAGTCAGGCAACTATGGATGAACG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4219..4242)
                     /label="1619"
                     /note="sequence: CTAATAATTCTTAGTTAAAAGCAC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(8776..8812)
                     /label="6383"
                     /note="sequence: tttgttttttgttttcttctaattgattttttctttc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          8637..8657
                     /label="1379"
                     /note="sequence: GTTACCCGACCTCGTTATTTT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          4443..4477
                     /label="B774 - GR-TPDNAPv3-rvs"
                     /note="sequence: TTATGTTGCcTTAACAGTtTTgTTTTCAATATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5773..5808)
                     /label="A739"
                     /note="sequence: TGAACATATCGTTAAtTTtACTAAAAATGATAAaTC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2427..2444)
                     /label="6941"
                     /note="sequence: ttcttcccgttccagcac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3609..3630
                     /label="1925"
                     /note="sequence: GCCGCAAATTCAAATATTTACG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(8089..8112)
                     /label="2034"
                     /note="sequence: gtgttgttatccgatacaaccgga"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7419..7441)
                     /label="320"
                     /note="sequence: CGATACTGCATTTCTAGGCATAT"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1158..1187
                     /label="A183"
                     /note="sequence: CAATTGCATGAAGAATCTGCTTAGGGTTAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
       61 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
      121 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
      181 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
      241 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
      301 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
      361 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
      421 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
      481 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
      541 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
      601 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
      661 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
      721 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
      781 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
      841 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
      901 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
      961 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT
     1021 CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG
     1081 CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA
     1141 AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG
     1201 CTTCGCGATG TACGGGCCAG ATATACGCGT TGgtgagtga attcTATATC TTTAACAGAT
     1261 TCCAAACCCT AAAGTGTCCG AATTTTCAAT AGGGCGAACT TGAAGAATAA CCAAGGTCAA
     1321 TAATATATCT TTTAGTATAA CCCTGAAATT TGCCCTATAG AAATCTAGGG TTTCTGTGTG
     1381 GTTTCCGGGT GAGTCATACG GCTTTTTTGA ATTTCTTTTT TTGCAGTTGT CTCTATCAAT
     1441 GAAAATTTCG AGGAAGACGA TAAGGTTAAG ATAAGTAGAT AAGAGAATGA TACGAGATAA
     1501 AGCACAAATT AGCAGAAAGA AGAGTGGTTG CGAACAGAGT AAACCGAATC AGGGAATCCC
     1561 TTTTTGCAAA AACATCAATT ATCCTTTTCT TTTTTTTACG TATATATCTG GAACAGAAAT
     1621 ATATAAGTTA CTATTATACT TATAGTTGGA TCCAGTTTTT AATCTGTCGT CAATCGAAAG
     1681 TTTATTTCAG AGTTCTTCAG ACTTCTTAAC TCCTGTAAAA ACAAAAAAAA AAAAAGGCAT
     1741 AGCAtgaccc aacatcagat gacccaaggt ccacctctta ttaaaggacg tttgatcctt
     1801 cgacaccatg gctctgttga acttttatct gagagaggaa aaaaaggaag gaaaaaaaag
     1861 aagaaacttc ctttatttat ttgtcttaac cacaacacac aatgcaataa gatgcaatat
     1921 aatatcaaag ccaatatctt atgttgctga tcctgagaag gaatatatac aatttatgta
     1981 gtaaaatacc ttttcttctg cgagttgcaa gaaatagaaa agactccgat tgcgcatcgc
     2041 cagaataaaa tttcacaacc acactttttg gctgaacttt ttattacctg attaaacaga
     2101 gagagaaaag gtagaggtca aaatttttta agcaaaacta aaaaagatgc aaaatcacgt
     2161 gctgaaaatc taacataagg gttaagatta gagttttata ggacttgttt tgtaatattt
     2221 caaatacgag ctaaccctac tgatttcaat taggtctaat ttagggttga gctgcactga
     2281 aatttcggaa attttgggtt attttaaatg agacagaaga actacagaga tacgttcttc
     2341 agactttaaa gcttatctcc acaaagaatt ggtcaagaaa tcatcctaga aaaacacgtt
     2401 tgctcactcg atcttaatca catagagtgc tggaacggga agaaATGACA AATTCAAAAG
     2461 AAGACGCCGA CATAGAGGAG AAGCATATGT ACAATGAGCC GGTCACAACC CTCTTTCACG
     2521 ACGTTGAAGC TTCACAAACA CACCACAGAC GTGGGTCAAT ACCATTGAAA GATGAGAAAA
     2581 GTAAAGAATT GTATCCATTG CGCTCTTTCC CGACGAGAGT AAATGGCGAG GATACGTTCT
     2641 CTATGGAGGA TGGCATAGGT GATGAAGATG AAGGAGAAGT ACAGAACGCT GAAGTGAAGA
     2701 GAGAGCTTAA GCAAAGACAT ATTGGTATGA TTGCCCTTGG TGGTACTATT GGTACAGGTC
     2761 TTTTCATTGG TTTATCCACA CCTCTGACCA ACGCCGGCCC AGTGGGCGCT CTTATATCAT
     2821 ATTTATTTAT GGGTTCTTTG GCATATTCTG TCACGCAGTC CTTGGGTGAA ATGGCTACAT
     2881 TCATCCCTGT TACATCCTCT TTCACAGTTT TCTCACAAAG ATTCCTTTCT CCAGCATTTG
     2941 GTGCGGCCAA TGGTTACATG TATTGGTTTT CTTGGGCAAT CACTTTTGCC CTGGAACTTA
     3001 GTGTAGTTGG CCAAGTCATT CAATTTTGGA CGTACAAAGT TCCACTGGCG GCATGGATTA
     3061 GTATTTTTTG GGTAATTATC ACAATAATGA ACTTGTTCCC TGTCAAATAT TACGGTGAAT
     3121 TtGAGTTCTG GGTCGCTTCC ATCAAAGTTT TAGCCATTAT CGGGTTTCTA ATATACTGTT
     3181 TTTGTATGGT TTGTGGTGCT GGGGTTACCG GCCCAGTTGG ATTCCGTTAT TGGAGAAACC
     3241 CAGGTGCCTG GGGTCCAGGT ATAATATCTA AGGATAAAAA CGAAGGGAGG TTCTTAGGTT
     3301 GGGTTTCCTC TTTGATTAAC GCTGCCTTCA CATTTCAAGG TACTGAACTA GTTGGTATCA
     3361 CTGCTGGTGA AGCTGCAAAC CCCAGAAAAT CCGTTCCAAG AGCCATCAAA AAAGTTGTTT
     3421 TCCGTATCTT AACCTTCTAC ATTGGCTCTC TATTATTCAT TGGACTTTTA GTTCCATACA
     3481 ATGACCCTAA ACTAACACAA TCTACTTCCT ACGTTTCTAC TTCTCCCTTT ATTATTGCTA
     3541 TTGAGAACTC TGGTACAAAG GTTTTGCCAC ATATCTTCAA CGCTGTTATC TTAACAACCA
     3601 TTATTTCTGC CGCAAATTCA AATATTTACG TTGGTTCCCG TATTTTATTT GGTCTATCAA
     3661 AGAACAAGTT GGCTCCTAAA TTCCTGTCAA GGACCACCAA AGGTGGTGTT CCATACATTG
     3721 CAGTTTTCGT TACTGCTGCA TTTGGCGCTT TGGCTTACAT GGAGACATCT ACTGGTGGTG
     3781 ACAAAGTTTT CGAATGGCTA TTAAATATCA CTGGTGTTGC AGGCTTTTTT GCATGGTTAT
     3841 TTATCTCAAT CTCGCACATC AGATTTATGC AAGCTTTGAA ATACCGTGGC ATCTCTCGTG
     3901 ACGAGTTACC ATTTAAAGCT AAATTAATGC CCGGCTTGGC TTATTATGCG GCCACATTTA
     3961 TGACGATCAT TATCATTATT CAAGGTTTCA CGGCTTTTGC ACCAAAATTC AATGGTGTTA
     4021 GCTTTGCTGC CGCCTATATC TCTATTTTCC TGTTCTTAGC TGTTTGGATC TTATTTCAAT
     4081 GCATATTCAG ATGCAGATTT ATTTGGAAGA TTGGAGATGT CGACATCGAT TCCGATAGAA
     4141 GAGACATTGA GGCAATTGTA TGGGAAGATC ATGAACCAAA GACTTTTTGG GACAAATTTT
     4201 GGAATGTTGT AGCATAAagt gcttttaact aagaattatt agtcttttct gcttattttt
     4261 tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt taggtttaaa
     4321 aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt gcataatgca
     4381 ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc agtttgaaaa
     4441 atTTATGTTG CcTTAACAGT tTTgTTTTCA ATATAACCTT TAACAGAACC CCATTCcCTT
     4501 CTAAACTTCA AGAATTGGAT CTTTGCTTCt TTATCAGCAC CAGACAACAA ATGAGCGTAC
     4561 AATTCTGGGA TGATaTAaTT TGATGGAATA CCcTTaAATC TcTTtTTTTC tTTAATACAT
     4621 GGcTTACCAT CTTGaAAaTA tGTaTAGAAA CCCATGTATT GtTTcTTACC TGCACACAAt
     4681 AAcTTATCAA ATTCACATTC cAATTCtAAT TGACCAACTT CtGAACCaAT aATTGGAACC
     4741 TTCAAACCaT TCAACATAAA tTTAGCATTT TTCTTGAAAT GTTCtTTGTG AACGAAGATA
     4801 GAATCTGTAT CaGAATATAT ACATTCTGCA CCTTCcTTTG CACCAGCATC CCAAATtGAT
     4861 TTCATGTGgT ATCTAGCCCA TGATAAtGTA AAAACACCaT TTTGGATTGG tTTAGATGAG
     4921 TATGTtGATT CTTTAATTTT CTTAATtAAC ATgaaATCAG TATCCCAGAT CTTgTAaGCT
     4981 TCTTCAGATT CaAAATCGAT ATCATCTTCa GATTTGATAA AaTATTCGAA GTTAACCCAt
     5041 TTTTGAGCAA ATTTACCCCA CAAAGAGTTC ATAATAATTT TAATAACATT TCTTTTAACC
     5101 TTATTATTTT GtTTTTCATA cTTtATCTTC AAcTTGTACA AAACATCtTT GATGTAcTTT
     5161 TCGAAGATcT TACCTTTCTT AGACCATGAa TAACCGATaT TACCgTAAGA ATCGAATGGG
     5221 ATaTATTCaA TGTTATGACC TTCaGACAAA CCGATATCaA TATCAACaTT aGTaTACCAA
     5281 GCGATGTAAG TAGATTCTTC AAAGTTaTTG TAtGTGATtT TgTTATGAGA TCTgATTGGA
     5341 AAGAAATTTG ACTTATTATT TCTATTTGGT GTAACTCTAA CaTAgTAGAT ACCtAATTCA
     5401 TCcTTTGGTC TGCTaGAAAC TCTTTTAAAt GAACCGTATG GTTGATCGTA GAATGCCATt
     5461 GAAGCTGGGT ATAAaGATTT AACATCTAAA CACAAAACAT CtTTATAaAT ACCATTAACT
     5521 GAAATACAcC TACCACCgAT tAAaGCTTCT CTCATaTTaA AGTAATCTTC tTTATTTGGA
     5581 TGTCTTAATT CAACATCaAA TGGCATaTTa GCTTGaAACA ATTTaTAAGA CAAACCtGCC
     5641 AATGTCATAG TTTGATCAAC TAATTCACAA TTGAAAATAT CTTCAACAGC GTTcTTgAAC
     5701 TCtAACCAAA CtTTTGATAA AACCAAAACA TCATTTCTAC AATAATCTTT AGACCATTCA
     5761 ATTAATGTTT TTGAtTTATC ATTTTTAGTa AAaTTAACGA TATGTTCAGA AGTAATTAAC
     5821 AATTTTTCTT TTTCAACAAC ATGATTAATT TCaGTaTTAA CAGATGACCA TTCtTTGTAA
     5881 ACCTTGTACA AATCATCAGC AGTCTTCAAA TCGTGATGTG GAAAATCtGT TTTtTTAAAt
     5941 GTTTCTTCaT TCAAAAAaGC TTTAGAtGCA TTAGCCAATG AACATAACAA AAAaGAGTAT
     6001 GAATCtTTAA CAGAAAATTT AACACCATCA TGaGCAAAgT TAAAtGAAAT gAAAGAaTTa
     6061 TTTCTGATtA AGATATTTTT AATaGCtGCT GGgTTATACA TAGAaGAtAA GATaTAATGG
     6121 AAATCGTAAC CACCACCatt ATGAGCAATT AATTCAACAG ATGAAATGTT tAATTCACCA
     6181 CAAATATCAA CAACaTATTC aATCATTTGa GCAACACAAT CTCTACCTTC GAAATCAACa
     6241 ATaTTACCgA TAACtTTgTT ATAGATAATA GAAGCACAAC AAATAAAAGG aATaTTAACT
     6301 TGaTTtGATT CtTTTTCTGG ATCGAAaTAT GATTCaATAT CaAAACAAAC tGTAATATTT
     6361 TTAACATCTG AAAATTTAGA CAATGGaGAA AAcTTTGTAA CTCTcCTATC ATGTCTaGAt
     6421 TTAGTAATAT TgAAAATAAC ACCtAAATGT TCTTTgAAaT AAAACAAgTA aGTGATtGTG
     6481 TTgTCAATAA CAGAgAAaGT cTTAATTGGg GATTCaGAGT ATAATCTgAT ATCATTAAAA
     6541 GATTTaATgT CTCTAACATC aTTaATATGt AATTGTGGAG TAACAATACA AATgTTACCg
     6601 TTtTTgTATT CGATCATTTC TTCcAATGTA TTAAAATCCT TTTGCCACAA gAATCTaGAA
     6661 CATTGtTTAA TACATGAAAC ATTATTCTTA TTTGCAGTAA ACAATTTAAA AGAATCGAAA
     6721 GTCAAaATGT TTGGAATATT ATTACCCCAa TTaTTAACAC ATCTTGGTTG TGGGAAATAG
     6781 ATaGAcTTAG CgTAGAAAAT AACaTAAGAG TATGTTTCTG AAACAAATAA AGATTCgTAA
     6841 CCgTAAGTAA CATCAATAAT TTTCTTCAAA ACATCATCAA AATTATTACA CAAgTCTTCt
     6901 AATCTTTCAT CAATtTTATC TTCgTAGATa TTAACtGAtT TAGTTGAAGC AATAACcTTA
     6961 CCtTTATTgA AAATCTTGAA gTACATgAAA GAAACTGATT GTCTAGAAAC ATTCTTGAAA
     7021 GAaGAtGATT CcTTAACCAT ATTtagGATc TTATTcTTCA ATGAGAAGAT gGTTTCcCTT
     7081 CTAAAACAtc tgatATTcAA ACCATCAAAA ATAAAAGGac gTGGTTCtAA AACCATATCT
     7141 TCtGAaGTtt cTTCAAAAGA gATTGAAACA CCcTTATGTC TtTTaATGTT TGGTAAAGAA
     7201 ATTCTTTCaG GgATTTCaGA TTGtTTCAAa TATTCTGCGT AcTTCTTAGC ATCTTCcTGT
     7261 CTTTCtAAAA TcTTcTTTTC AACATCTTCt TTTTGTAACA AaGTtGCTCT ATCTTTAATC
     7321 AAgATTTGTC TgAAATCTAA TGGtAAAGAg TCGAAgTCTG CaTAAACgTT TCTCAAATCG
     7381 TTCAATGCcT TgTCCTTgTA ATCCATagat ctcgctggat atgcctagaa atgcagtatc
     7441 gatttatttt gagaaaatct gtcaactcgt tacttctttg atagggaaat ctttagttaa
     7501 atttgtttaa aattaagaac caggtcttta gactatgcca aaaggtattg gtgcttaaag
     7561 gttgccgtaa actctaactc tatatatctc taagccaatg tttaagttga tgcgatgtgc
     7621 atgatcactt cacatttttt tcatgatttt gttttcttcc ttagctactt ttttccgtct
     7681 tggtctgtgt aaaaaaaaaa tcgcttcact gaagaaaaat ataaataaaa aaaggacatc
     7741 agcaagttcg tagtcaataa atgtgatgag taatttgcga taatctaccc caactcaaag
     7801 cattcaattc aaatgaaaga agagttcgtg aattttaatc tggcttacag attggatcat
     7861 agcttctaat atatcttcct ttttatttta aagtgattaa aatatgtgga tgtctgcatt
     7921 aaggtgaagg cagactatag tgaaaaatag tagcaaaggc ttcatataat gaactaccta
     7981 gttgtattca attggaatct gtgaacgcgg cattaaaaga atatttgagc tttttttgat
     8041 tgtattaacc tgaagctatc acggtttaga ctcattaaaa gaaaaatatc cggttgtatc
     8101 ggataacaac acaagaggat gtccaatatt ttttttaagg aataaggata cttcaagact
     8161 agattccccc ctgcattccc atcagaaccg taaaccttgg cgctttcctt gggaagtatt
     8221 caagaagtgc cttgtccggt ttctgtggct cacaaaccag cgcgcccgat atggctttct
     8281 tttcacttat gaatgtacca gtacgggaca attagaacgc tcctgtaaca atctctttgc
     8341 aaatgtgggg ttacattcta accatgtcac actgctgacg aaattcaaag taaaaaaaaa
     8401 tgggaccacg tcttgagaac gatagatttt ctttatttta cattgaacag tcgttgtctc
     8461 agcgcgcttt atgttttcat tcatacttca tattataaaa taacaaaaga agaatttcat
     8521 attcacgccc aagaaatcag gctgctttcc aaatgcaatt gacacttcat tagccatcac
     8581 acaaaactct ttcttgctgg agcttctttt aaaaaagacc tcagtacacc aaacacgtta
     8641 cccgacctcg ttattttacg acaactatga taaaattctg aagaaaaaat aaaaaaattt
     8701 tcatacttct tgcttttatt taaaccattg aatgatttct tttgaacaaa actacctgtt
     8761 tcaccaaagg aaatagaaag aaaaaatcaa ttagaagaaa acaaaaaaca aaatgggtaa
     8821 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     8881 gtccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     8941 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     9001 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     9061 atttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     9121 cctgcctgaa accgaactgc ccgctgttct gcaaccggtc gcggaggcca tggatgcgat
     9181 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     9241 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     9301 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     9361 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     9421 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     9481 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     9541 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     9601 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     9661 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     9721 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     9781 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     9841 atagggataa cagggtaatA TATGACGTTT TATTACCTTT GATCACATTT CCACGCCATT
     9901 TCGCATTCTC ACCCTCATAA GTCATACACC GAAAAGAAAG TTTAAGGGAT CAATGAGCTT
     9961 ACTATAATCT CAGTATATTT ATTTTTATCG ATGATTCACC ACAACAATCT TGCTCCCGAA
    10021 AAGAAAGCAG ACGGAGTAGA AGCATTTGAA ACTCCTTCAG ACCTTCAAGT ATATATATAT
    10081 ATATATATAT ATGTATATGT GTACATTTTC ACGCTAATAC TAATGTATAA TTAGAAGATA
    10141 ATTTTTACTC ATTTTTCGTT ATCTTCACGT CACCCGAACC TAGAACCAAA TGTCATTTTC
    10201 ACGATATGTA AATAGTGAAA TAGGCAAAAA CGCCAAAAAG TAGTAAGCGC AACATACACT
    10261 AAACCATTAA AGAATATCTC GACCAGAATC TAACAGATAT ACATGTTCCG ATAATGTCTG
    10321 AGTTAGGTGA GTATTCTAAA TTAGAAAACA AAGAGCTTAg aattcCCGTC GACCTCTAGC
    10381 TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
    10441 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
    10501 AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
    10561 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
    10621 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA
    10681 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
    10741 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
    10801 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
    10861 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
    10921 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
    10981 AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
    11041 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
    11101 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
    11161 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
    11221 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
    11281 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
    11341 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
    11401 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
    11461 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
    11521 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA G
//