LOCUS       Exported                7398 bp DNA     circular SYN 15-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Fishell Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=L-ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    108..1203
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_G_EGFP-N"
     misc_feature    132..144
                     /label=Gibson_hDlx_P_F
     misc_feature    139
                     /label=A from MluI
     misc_feature    140..150
                     /label=Reconstituted MluI
     misc_feature    145..150
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_G_EGFP-N"
     misc_feature    151..908
                     /label=S9E10
     primer_bind     complement(755..779)
                     /label=Seq-E10.5R
     misc_feature    909..918
                     /label=Reconstituted PmeI
     misc_feature    909..914
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_G_EGFP-N"
     misc_feature    919
                     /label=A from PmeI
     promoter        938..986
                     /label=pB-Globin
     primer_bind     938..959
                     /label=Seq-pGlo.5F
     primer_bind     complement(941..960)
                     /label=AAV_hDlx_R1
     primer_bind     complement(950..967)
                     /label=Seq-bGlobin.R
                     /note="58.2"
     misc_feature    1033..1203
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_R_140619_Seq_AAV_Dlx_R"
     intron          1071..1203
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1142..1161
                     /label=Seq-bGlo-intronF
     primer_bind     complement(1142..1161)
                     /label=Seq-bGlo-intronR
     primer_bind     1170..1189
                     /label=Seq_AAV_hDlx_F3
     primer_bind     complement(1184..1218)
                     /label=S5BB_Dlx_Gibs_R
     primer_bind     1207..1231
                     /label=GA-SV15.F
     misc_feature    1207..1211
                     /label=Fa
     primer_bind     1211..1272
                     /label=Gibson_HA-NLS_F1
     misc_feature    1212..3850
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_R_140619_Seq_AAV_Dlx_R"
     misc_feature    1217..1222
                     /label=Kozak
     misc_feature    1219..3832
                     /label=Insert_4
     CDS             1223..3019
                     /codon_start=1
                     /product="From Roth LabHA tag added
                     "
                     /label=HA-GqDREADD
                     /translation="MYPYDVPDYATLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTH
                     FGSYNVSRAAGNFSSPDGTTDDPLGGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKV
                     NKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDCVASNA
                     SVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGK
                     RTVPPGECFIQFLSEPTITFGTAIAGFYMPVTIMTILYWRIYKETEKRTKELAGLQASG
                     TEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDH
                     SSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQ
                     VPEEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGK
                     STATLPLSFKEATLAKRFALKTRSQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYN
                     IMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLLLCQCDKKK
                     RRKQQYQQRQSVIFHKRAPEQAL"
     misc_feature    1226..1252
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_R_140619_Seq_AAV_Dlx_R"
     misc_feature    1253..1272
                     /label=Gibson_NLS_F1b
     primer_bind     complement(1433..1451)
                     /label=Seq-Gq5R
                     /note="56"
     primer_bind     1632..1651
                     /label=Seq_AAV_hDlx_F4
     primer_bind     2161..2180
                     /label=Seq_AAV_hDlx_F5
     primer_bind     complement(2290..2307)
                     /label=Seq-Gq-midR
                     /note="59"
     misc_feature    2290
                     
     misc_feature    2338
                     /label=Missing in Clone 3 F
                     /note="Could be double peak on Raw"
     misc_feature    2419
                     /label=End of sequencing Clone1
     misc_feature    2614
                     /label=End of sequencing Clone 1 R
     primer_bind     2753..2772
                     /label=Seq_AAV_hDlx_F6
     primer_bind     2961..2980
                     /label=Seq_AAV_hDlx_F7
     primer_bind     complement(3000..3045)
                     /label=Gibson_NLS_R1
     primer_bind     3000..3045
                     /label=Gibson_NLS_F2
     misc_feature    3000..3019
                     /label=Gibson_NLS_R1
     misc_feature    3007..3019
                     /label=Overlap Gibson 2A
     misc_feature    3017..3019
                     /label=150212_AAV_hDLX_F7_Sequenced
                     /note="Ok for the 12 minis"
     misc_feature    3020..3025
                     /label=BspEI
     misc_feature    3026..3045
                     /label=Gibson_NLS_F2
     CDS             3032..3097
                     /codon_start=1
                     /label=P2A
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             3110..3130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3131..3832
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3132..3148)
                     /label=Seq-GFP5-R
     primer_bind     complement(3135..3152)
                     /label=Seq-dTom5R
                     /note="60"
     primer_bind     complement(3172..3191)
                     /label=Seq-mCherry5-R
                     /note="62"
     primer_bind     3808..3826
                     /label=Seq-YFP3.F
                     /note="59"
     primer_bind     complement(3810..3859)
                     /label=GA-SV14.R
     primer_bind     complement(3813..3859)
                     /label=GA-SV13.R
     primer_bind     complement(3821..3859)
                     /label=GA-SV15.R
     misc_feature    3825..3844
                     /label=Gibson_NLS_R2
     misc_feature    3834..3839
                     /label=140708_Sequencing_Clone1
                     /note="Check 140708_AAV_GFP_1_R_140619_Seq_AAV_Dlx_R"
     misc_feature    3834..3839
                     /label=Gibson_NLS_R2
     misc_feature    3841..3859
                     /label=CUT OUT
     misc_feature    3845..3864
                     /label=Gibson_NLS_R2b
     misc_feature    3847..3862
                     /label=Gibson_R_16bp
     misc_feature    3848
                     /label=begening of sequencing CLone1 with R
     primer_bind     complement(3873..3892)
                     /label=Seq_AAV_Dlx_R
                     /note="agcttatcgataatcaacct"
     misc_feature    3884..4472
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3950..3969)
                     
     primer_bind     3986..4007
                     /label=WPRE2.F
                     /note="22 mer	59"
     primer_bind     4149..4168
                     /label=WPRE1.F
                     /note="20 mer	59.67"
     primer_bind     4239..4257
                     /label=WPRE_Titration_F
     primer_bind     4239..4257
                     /label=WPRE_qPCR_F
     primer_bind     complement(4248..4267)
                     /label=WPRE1.R
                     /note="20 mer	59.76"
     primer_bind     4272..4291
                     /label=Seq_AAV_hDlx_F10
     primer_bind     complement(4276..4297)
                     /label=WPRE_Titration_R
     primer_bind     complement(4276..4297)
                     /label=WPRE_qPCR_R
     polyA_signal    4501..4582
                     /label=SV40pA
                     /note="SV40 polyadenylation signal"
     primer_bind     4560..4579
                     /label=Seq_AAV_hDlx_F11
     primer_bind     complement(4621..4641)
                     /label=Seq-SV40pA-R
                     /note="56.4"
     repeat_region   4661..4801
                     /label=R-ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4876..5331
                     /direction=RIGHT
                     /label=F1 Origin
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5613..5717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5718..6578
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6749..7337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7355..7374
                     /label=Seq_AAV_hDlx_F1
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat ctaggttcct gtctgcaagc atagcagggt
      181 atcattaata gtgtcaggag ttggctctct cccataggat gggtctcaag ttggggcaat
      241 cattggttgg ctgatccctt ggtctctgtc ccatctttat tcctgcacat cttgtaggca
      301 ggacaaattt tggtttgttc caaagtgatt tttaaagcat taccattttg tgttttgttg
      361 gtttaaaaca ctttagctat tctggtgggt gcgtgatcct gcctcactac ggtctcagtc
      421 tccatctcga tggtgactaa ggatgcctcc tatgcactta tttaccatct gaatggttcc
      481 tttatagaat gcatgactat tcaatttttt tgctcattgg acagcatttt aatgtgtgaa
      541 aggattatac atccttcaag atatggaaga aaaagcacaa agccctaaat tctgtgaatt
      601 cagatgtgta aaaaaagaca aggaagtgca aactatatca gcttggtgtc tcattctggg
      661 tggctgtcag agccctgtga gctgacttaa gaattgggtc cctgcaatgg gtgctggcac
      721 ttgcaagggg atcatgagct caaggccata ctgagctgca taatgagacc ttgtctcagt
      781 aactagactg gggttggagg tcagtactaa agggctagcc tagcctaaat tcccgagttc
      841 cacccccagc actataaaag gaaaaggagg aagaggtgga gggaaataaa gaaaaggaaa
      901 gccagtgtat cgatgtttaa acttcctgca gcccgggctg ggcataaaag tcagggcaga
      961 gccatctatt gcttacattt gcttctagcc tgcaagtcga ggagcgcagc cttccagaag
     1021 cagagcgcgg cgccttaagc tgcagaagtt ggtcgtgagg cactgggcac gtaagtatca
     1081 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     1141 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1201 cagcgagctc gctagcgcca ccatgtaccc atacgatgtt ccagattacg ctaccttgca
     1261 caataacagt acaacctcgc ctttgtttcc aaacatcagc tcctcctgga tacacagccc
     1321 ctccgatgca gggctgcccc cgggaaccgt cactcatttc ggcagctaca atgtttctcg
     1381 agcagctggc aatttctcct ctccagacgg taccaccgat gaccctctgg gaggtcatac
     1441 cgtctggcaa gtggtcttca tcgctttctt aacgggcatc ctggccttgg tgaccatcat
     1501 cggcaacatc ctggtaattg tgtcatttaa ggtcaacaag cagctgaaga cggtcaacaa
     1561 ctacttcctc ttaagcctgg cctgtgccga tctgattatc ggggtcattt caatgaatct
     1621 gtttacgacc tacatcatca tgaatcgatg ggccttaggg aacttggcct gtgacctctg
     1681 gcttgccatt gactgcgtag ccagcaatgc ctctgttatg aatcttctgg tcatcagctt
     1741 tgacagatac ttttccatca cgaggccgct cacgtaccga gccaaacgaa caacaaagag
     1801 agccggtgtg atgatcggtc tggcttgggt catctccttt gtcctttggg ctcctgccat
     1861 cttgttctgg caatactttg ttggaaagag aactgtgcct ccgggagagt gcttcattca
     1921 gttcctcagt gagcccacca ttacttttgg cacagccatc gctggttttt atatgcctgt
     1981 caccattatg actattttat actggaggat ctataaggaa actgaaaagc gtaccaaaga
     2041 gcttgctggc ctgcaagcct ctgggacaga ggcagagaca gaaaactttg tccaccccac
     2101 gggcagttct cgaagctgca gcagttacga acttcaacag caaagcatga aacgctccaa
     2161 caggaggaag tatggccgct gccacttctg gttcacaacc aagagctgga aacccagctc
     2221 cgagcagatg gaccaagacc acagcagcag tgacagttgg aacaacaatg atgctgctgc
     2281 ctccctggag aactccgcct cctccgacga ggaggacatt ggctccgaga cgagagccat
     2341 ctactccatc gtgctcaagc ttccgggtca cagcaccatc ctcaactcca ccaagttacc
     2401 ctcatcggac aacctgcagg tgcctgagga ggagctgggg atggtggact tggagaggaa
     2461 agccgacaag ctgcaggccc agaagagcgt ggacgatgga ggcagttttc caaaaagctt
     2521 ctccaagctt cccatccagc tagagtcagc cgtggacaca gctaagactt ctgacgtcaa
     2581 ctcctcagtg ggtaagagca cggccactct acctctgtcc ttcaaggaag ccactctggc
     2641 caagaggttt gctctgaaga ccagaagtca gatcactaag cggaaaagga tgtccctggt
     2701 caaggagaag aaagcggccc agaccctcag tgcgatcttg cttgccttca tcatcacttg
     2761 gaccccatac aacatcatgg ttctggtgaa caccttttgt gacagctgca tacccaaaac
     2821 cttttggaat ctgggctact ggctgtgcta catcaacagc accgtgaacc ccgtgtgcta
     2881 tgctctgtgc aacaaaacat tcagaaccac tttcaagatg ctgctgctgt gccagtgtga
     2941 caaaaaaaag aggcgcaagc agcagtacca gcagagacag tcggtcattt ttcacaagcg
     3001 cgcacccgag caggccttgt ccggagataa cggaagcgga gccactaact tctccctgtt
     3061 gaaacaagca ggggatgtcg aagagaatcc cgggccatct agaatggccc ccaagaagaa
     3121 gaggaaggtg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     3181 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3241 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     3301 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     3361 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3421 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     3481 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     3541 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     3601 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     3661 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     3721 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     3781 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt aagtcgacgg
     3841 cgcgccccac ccctgcaggg aattcgatat caagcttatc gataatcaac ctctggatta
     3901 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3961 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4021 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4081 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4141 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4201 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4261 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     4321 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4381 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4441 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     4501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatca
     4621 gcttatcgat accgcatgca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4681 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4741 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4801 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4861 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4921 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     4981 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5041 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5101 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5161 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     5221 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     5281 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5341 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5401 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5461 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5521 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5581 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7381 ggccttttgc tcacatgt
//
