LOCUS       pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast 12143 bp ds-DNA     circular     18-DEC-2023
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             771..864
                     /label="lacI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
                     /translation="SAQYANRLSPRVGRFINAAGTTGFPTGKRAV"
     protein_bind    complement(877..898)
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     promoter        913..943
                     /label="lac promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(947..969)
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     promoter        complement(1012..1179)
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.405"
                     /match_length="85.71428571428571"
                     /fragment="True"
                     /other="promoter"
     polyA_signal    1189..1320
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.507"
                     /match_length="97.8102189781022"
                     /fragment="False"
                     /other="polyA signal"
     LTR             complement(1392..1625)
                     /label="3' LTR (Delta-U3)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(1627..1646)
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="17.094017094017094"
                     /fragment="True"
                     /other="misc feature"
     misc_feature    1699..2287
                     /label="WPRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.831"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     CDS             complement(2302..2700)
                     /label="BSD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG*"
     CDS             complement(2701..2754)
                     /label="T2A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2764..4413)
                     /label="luciferase"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.758"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     LTR             complement(4448..4716)
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.63"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        complement(4729..4958)
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.565"
                     /match_length="19.776440240756664"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(5000..6835)
                     /label="PRDM6-V5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(5000..6835)
                     /label="PRDM6-V5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MLKPGDPGGSAFLKVDPAYLQHWQQLFPHGGAGPLKGSGAAGLLSAPQPLQPPPPPPPPERAEPPPDSLRPRPASLSSASSTPASSSTSASSASSCAAAAAAAALAGLSALPVSQLPVFAPLAAAAVAAEPLPPKELCLGATSGPGPVKCGGGGGGGGEGRGAPRFRCSAEELDYYLYGQQRMEIIPLNQHTSDPNNRCDMCADNRNGECPMHGPLHSLRRLVGTSSAAAAAPPPELPEWLRDLPREVCLCTSTVPGLAYGICAAQRIQQGTWIGPFQGVLLPPEKVQAGAVRNTQHLWEIYDQDGTLQHFIDGGEPSKSSWMRYIRCARHCGEQNLTVVQYRSNIFYRACIDIPRGTELLVWYNDSYTSFFGIPLQCIAQDENLNVPSTVMEAMCRQDALQPFNKSSKLAPTTQQRSVVFPQTPCSRNFSLLDKSGPIESGFNQINVKNQRVLASPTSTSQLHSEFSDWHLWKCGQCFKTFTQRILLQMHVCTQNPDRPYQCGHCSQSFSQPSELRNHVVTHSSDRPFKCGYCGRAFAGATTLNNHIRTHTGEKPFKCERCERSFTQATQLSRHQRMPNECKPITESPESIEVDGGGKPIPNPLLGLDST*"
     CDS             complement(5003..5044)
                     /label="V5 tag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="GKPIPNPLLGLDST"
     intron          complement(6901..7917)
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.902"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     promoter        complement(7918..8201)
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.944"
                     /match_length="101.067615658363"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(8203..8567)
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="96.30606860158312"
                     /fragment="False"
                     /other="enhancer"
     misc_feature    complement(8585..8702)
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     ncRNA           complement(9144..9479)
                     /label="RRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
     ncRNA           complement(9790..9872)
                     /label="HIV GSL3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
     misc_feature    complement(9924..10049)
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.438"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     LTR             complement(10096..10276)
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        complement(10277..10503)
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             10517..10677
                     /label="lacZ"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.235772357723578"
                     /fragment="True"
                     /other="CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*MANG"
     misc_feature    complement(10677..10776)
                     /label="bom"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="71.94244604316546"
                     /fragment="True"
                     /other="misc feature"
     CDS             complement(10878..10913)
                     /label="rop"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="19.047619047619047"
                     /fragment="True"
                     /other="CDS"
                     /translation="LARFGDDGENL*"
     promoter        11008..11112
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             11113..11973
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aatttggtct ctgcaaaagt
     1021 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     1081 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     1141 gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata
     1201 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1261 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1321 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     1381 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     1441 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     1501 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     1561 ccagagagac ccagtacaag caaaaagcag atcttatctt cgttgggagt gaattagccc
     1621 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     1681 tggtcttaaa ggtaccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     1741 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     1801 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     1861 cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag
     1921 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     1981 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     2041 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     2101 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     2161 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     2221 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     2281 gttgattggc gcgccgtcga cttagccctc ccacacataa ccagagggca gcaattcacg
     2341 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     2401 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     2461 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     2521 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     2581 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     2641 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     2701 aggtccagga ttctcctcga cgtcaccgca tgttagcaga cttcctctgc cctctccgct
     2761 tcccacggcg atcttgccgc ccttcttggc ctttatgagg atctctctga tttttcttgc
     2821 gtcgagtttt ccggtaagac ctttcggtac ttcgtccaca aacacaactc ctccgcgcaa
     2881 ctttttcgcg gttgttactt gactggcgac gtaatccacg atctcttttt ccgtcatcgt
     2941 ctttccgtgc tccaaaacaa caacggcggc gggaagttca ccggcgtcat cgtcgggaag
     3001 acctgcgaca cctgcgtcga agatgttggg gtgttggagc aagatggatt ccaattcagc
     3061 gggagccacc tgatagcctt tgtacttaat cagagacttc aggcggtcaa cgatgaagaa
     3121 gtgttcgtct tcgtcccagt aagctatgtc tccagaatgt agccatccat ccttgtcaat
     3181 caaggcgttg gtcgcttccg gattgtttac ataaccggac ataatcatag gacctctcac
     3241 acacagttcg cctctttgat taacgcccag cgttttcccg gtatccagat ccacaacctt
     3301 cgcttcaaaa aatggaacaa ctttaccgac cgcgcccggt ttatcatccc cctcgggtgt
     3361 aatcagaata gctgatgtag tctcagtgag cccatatcct tgcctgatac ctggcagatg
     3421 gaacctcttg gcaaccgctt ccccgacttc cttagagagg ggagcgccac cagaagcaat
     3481 ttcgtgtaaa ttagataaat cgtatttgtc aatcagagtg cttttggcga agaaggagaa
     3541 tagggttggc accagcagcg cactttgaat cttgtaatcc tgaaggctcc tcagaaacag
     3601 ctcttcttca aatctataca ttaagacgac tcgaaatcca catatcaaat atccgagtgt
     3661 agtaaacatt ccaaaaccgt gatggaatgg aacaacactt aaaatcgcag tatccggaat
     3721 gatttgattg ccaaaaatag gatctctggc atgcgagaat ctcacgcagg cagttctatg
     3781 aggcagagcg acacctttag gcagaccagt agatccagag gagttcatga tcagtgcaat
     3841 tgtcttgtcc ctatcgaagg actctggcac aaaatcgtat tcattaaaac cgggaggtag
     3901 atgagatgtg acgaacgtgt acatcgactg aaatccctgg taatccgttt tagaatccat
     3961 gataataatt ttttggatga ttgggagctt tttttgcacg ttcaaaattt tttgcaaccc
     4021 ctttttggaa acgaacacca cggtaggctg cgaaatgccc atactgttga gcaattcacg
     4081 ttcattataa atgtcgttcg cgggcgcaac tgcaactccg ataaataacg cgcccaacac
     4141 cggcataaag aattgaagag agttttcact gcatacgacg attctgtgat ttgtattcag
     4201 cccatatcgt ttcatagctt ctgccaaccg aacggacatt tcgaagtact cagcgtaagt
     4261 gatgtccacc tcgatatgtg catctgtaaa agcaattgtt ccaggaacca gggcgtatct
     4321 cttcatagcc ttatgcagtt gctctccagc ggttccatct tccagcggat agaatggcgc
     4381 cgggcccttc ttaatgtttt tggcatcttc catggtggcg tctagcgtag gcgccggtca
     4441 cagcttggat ctgtaacggc gcagaacaga aaacgaaaca aagacgtaga gttgagcaag
     4501 cagggtcagg caaagcgtgg agagccggct gagtctaggt aggctccaag ggagcgccgg
     4561 acaaaggccc ggtctcgacc tgagctttaa acttacctag acggcggacg cagttcagga
     4621 ggcaccacag gcgggaggcg gcagaacgcg actcaaccgg cgtggatggc ggcctcaggt
     4681 agggcggcgg gcgcgtgaag gagagatgcg agcccctcga agcttcagct gtgttctggc
     4741 ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc
     4801 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
     4861 ccaccttctc taggcacccg ttcaattgcc gacccctccc cccaacttct cggggactgt
     4921 gggcgatgtg cgctctgccc actgacgggc accggagcga tcgcagatcc ttcgcggccg
     4981 cttacatggt ggcgaattct caggtagagt cgagtcccag aaggggatta gggattggct
     5041 tcccacctcc atccacttcg attgattctg ggctctcagt tattggcttg cactcattgg
     5101 gcatccgctg gtgtcggctc agctgggtgg cctgcgtgaa gctcctctca cacctctcgc
     5161 acttgaaggg cttttctcca gtgtgggttc ggatgtggtt gttgagggtg gtggccccgg
     5221 caaaggcacg accacagtag ccgcacttga aaggccggtc actagagtga gtgaccacgt
     5281 ggttcctcag ttctgaaggc tgggaaaagg actgggagca gtggccgcat tggtagggtc
     5341 tgtcggggtt ctgcgtgcac acgtgcatct gtaagaggat ccgctgggtg aaagtcttaa
     5401 agcactgccc acatttccaa agatgccagt cactgaactc cgagtggagc tggcttgtgg
     5461 aagttgggct tgccaggact cgctggtttt tcacgttgat ttgattaaat cctgattcaa
     5521 tgggcccaga cttatccaga agagagaagt tcctgctgca cggagtctgg gggaaaacaa
     5581 cggagcgctg ctgggtggta ggggcgagtt tgctgctttt gttgaagggc tgcagggcgt
     5641 cttgtctgca catggcttcc attaccgttg aagggacatt taagttttca tcctgggcaa
     5701 tgcattgtaa ggggatccca aagaaagacg tatagctgtc attgtaccac accagaagct
     5761 cggtgcccct agggatatct atacaggctc ggtagaatat attcgacctg tactgaacta
     5821 ctgttagatt ctgttctccg cagtgccttg cacatcggat atacctcatc cagctcgact
     5881 tactaggttc cccaccatca ataaagtgct gtagtgtccc atcctggtca tatatctccc
     5941 agagatgctg cgtgttcctc acggcgcctg cctgcacctt ctctgggggc agaagcacgc
     6001 cttggaaagg tccaatccag gtgccttgct ggatcctctg cgccgcgcag atgccgtagg
     6061 ccaggccggg cacagtactg gtgcagaggc acacctcgcg aggcaggtcc cgcagccact
     6121 ccggcagctc cggcgggggc gcggcggccg cagcgctgct ggtgcccaca agccggcgca
     6181 gcgagtgcag tggcccatgc atagggcact cgccgttgcg gttgtccgcg cacatgtcgc
     6241 aacggttgtt ggggtcgctg gtgtgctggt tgagcgggat gatctccatg cgctgctggc
     6301 catacaggta atagtccagc tcctctgcgc tgcagcggaa gcgcggggcg ccgcgaccct
     6361 ccccgccgcc gccgccacca ccaccgcact tgacgggccc ggggccggag gtggcgccga
     6421 ggcacagttc cttggggggc agcggctcgg cggcgacggc agcggcggct agaggcgcga
     6481 acaccggcag ctgcgacacc ggcagggccg agagaccagc cagcgcggcg gcagcggccg
     6541 cagcagcgca ggaggaggcg gaggaggcgg aggtggaaga ggaagccggc gtggacgagg
     6601 cggaggagag agaggcgggc cgcgggcgca ggctgtccgg cggaggctca gcgcgctccg
     6661 ggggcggggg cggcggcggc ggctgaagag gctgcggcgc gctcaggaga cccgcggcgc
     6721 cgctgccctt gagcgggcct gcgcctccgt gagggaagag ttgctgccag tgctgcaggt
     6781 aggctgggtc cactttgagg aaggccgaac cgccggggtc tccgggcttc agcatggtgg
     6841 cttaattaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
     6901 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
     6961 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
     7021 acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc
     7081 cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt
     7141 gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc
     7201 cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc
     7261 ggcgctccgg gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc
     7321 cccgcccggc acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg
     7381 ccccgtacgg agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg
     7441 cagggggggt tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac
     7501 accccgcacg cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc
     7561 ggccgcgctc ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     7621 ctcacagccg ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca
     7681 cgcacccccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
     7741 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
     7801 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     7861 gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc
     7921 ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa
     7981 ctttcggagc gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg
     8041 cccctcgccc cgccccgccc cgcctggcgc gcgccccccc cccccccccc ccccgccccc
     8101 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
     8161 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac
     8221 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     8281 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     8341 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     8401 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     8461 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     8521 tgggctatga actaatgacc ccgtaattga ttactattaa taactagcta gtgatatcat
     8581 cgataaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
     8641 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
     8701 aagttaaccg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     8761 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     8821 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     8881 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     8941 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     9001 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     9061 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     9121 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     9181 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     9241 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     9301 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     9361 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     9421 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     9481 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     9541 aattgtccct catatctcct cctccaggtc tgaagatcag tggccgcttg ctgtgcggtg
     9601 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     9661 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     9721 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     9781 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     9841 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     9901 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     9961 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
    10021 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
    10081 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
    10141 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
    10201 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
    10261 ctaaccagag agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat
    10321 gcggcaattc agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg
    10381 cacgatcgta ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta
    10441 aggcatgttg ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact
    10501 acacgcgtca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
    10681 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    10741 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    10801 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10861 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    10921 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10981 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11101 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    11161 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    11221 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11281 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11341 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11401 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11461 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11521 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    11581 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    11641 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    11701 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11761 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11821 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11881 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11941 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12001 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12061 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12121 gtagaaaaga tcaaaggatc ttc
//