LOCUS       hsKIF5B(1-560)-HaloTag- 8924 bp ds-DNA     circular     14-DEC-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            KJ060_hkif5b(1-560)-HaloTag-FRB (1).ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    13..1692
                     /label="hKif5b(1-560)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="hKif5b(1-560)"
                     /label="hKif5b(1-560)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1690..1692
                     /label="residue 560"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="residue 560"
                     /label="residue 560"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             1702..2586
                     /label="HaloTag(R)"
                     /ApEinfo_revcolor="#b90746"
                     /ApEinfo_fwdcolor="#b90746"
                     /product="modified bacterial dehalogenase that formscovalent bonds with chloroalkane derivatives"
                     /locus_tag="HaloTag(R)"
                     /label="HaloTag(R)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISG"
     misc_feature    2593..2871
                     /label="FRB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="FRB"
                     /label="FRB"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2872..2874
                     /label="STOP"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /locus_tag="STOP"
                     /label="STOP"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 atgggcgcgc ctAtggcgga cctggccgag tgcaacatca aagtgatgtg tcgcttcaga
       61 cctctcaacg agtctgaagt gaaccgcggc gacaagtaca tcgccaagtt tcagggagaa
      121 gacacggtcg tgatcgcgtc caagccttat gcatttgatc gggtgttcca gtcaagcaca
      181 tctcaagagc aagtgtataa tgactgtgca aagaagattg ttaaagatgt acttgaagga
      241 tataatggaa caatatttgc atatggacaa acatcctctg ggaagacaca cacaatggag
      301 ggtaaacttc atgatccaga aggcatggga attattccaa gaatagtgca agatattttt
      361 aattatattt actccatgga tgaaaatttg gaatttcata ttaaggtttc atattttgaa
      421 atatatttgg ataagataag ggacctgtta gatgtttcaa agaccaacct ttcagttcat
      481 gaagacaaaa accgagttcc ctatgtaaag gggtgcacag agcgttttgt atgtagtcca
      541 gatgaagtta tggataccat agatgaagga aaatccaaca gacatgtagc agttacaaat
      601 atgaatgaac atagctctag gagtcacagt atatttctta ttaatgtcaa acaagagaac
      661 acacaaacgg aacaaaagct gagtggaaaa ctttatctgg ttgatttagc tggtagtgaa
      721 aaggttagta aaactggagc tgaaggtgct gtgctggatg aagctaaaaa catcaacaag
      781 tcactttctg ctcttggaaa tgttatttct gctttggctg agggtagtac atatgttcca
      841 tatcgagata gtaaaatgac aagaatcctt caagattcat taggtggcaa ctgtagaacc
      901 actattgtaa tttgctgctc tccatcatca tacaatgagt ctgaaacaaa atctacactc
      961 ttatttggcc aaagggccaa aacaattaag aacacagttt gtgtcaatgt ggagttaact
     1021 gcagaacagt ggaaaaagaa gtatgaaaaa gaaaaagaaa aaaataagat cctgcggaac
     1081 actattcagt ggcttgaaaa tgagctcaac agatggcgta atggggagac ggtgcctatt
     1141 gatgaacagt ttgacaaaga gaaagccaac ttggaagctt tcacagtgga taaagatatt
     1201 actcttacca atgataaacc agcaaccgca attggagtta taggaaattt tactgatgct
     1261 gaaagaagaa agtgtgaaga agaaattgct aaattataca aacagcttga tgacaaggat
     1321 gaagaaatta accagcaaag tcaactggta gagaaactga agacgcaaat gttggatcag
     1381 gaggagcttt tggcatctac cagaagggat caagacaata tgcaagctga gctgaatcgc
     1441 cttcaagcag aaaatgatgc ctctaaagaa gaagtgaaag aagttttaca ggccctagaa
     1501 gaacttgctg tcaattatga tcagaagtct caggaagttg aagacaaaac taaggaatat
     1561 gaattgctta gtgatgaatt gaatcagaaa tcggcaactt tagcgagtat agatgctgag
     1621 cttcagaaac ttaaggaaat gaccaaccac cagaaaaaac gagcagctga gatgatggca
     1681 tctttactaa aaaagtcgac tgaaatcggt actggctttc cattcgaccc ccattatgtg
     1741 gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg
     1801 ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt
     1861 gcaccgaccc atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca
     1921 gacctgggtt atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg
     1981 ggtctggaag aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg
     2041 gccaagcgca atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc
     2101 ccgacctggg acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc
     2161 gacgtcggcc gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg
     2221 ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat
     2281 cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca
     2341 gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg
     2401 aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg
     2461 gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa
     2521 gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtccac gctcgagatt
     2581 tccggcacta gtATCCTCTG GCATGAGATG TGGCATGAAG GCCTGGAAGA GGCATCTCGT
     2641 TTGTACTTTG GGGAAAGGAA CGTGAAAGGC ATGTTTGAGG TGCTGGAGCC CTTGCATGCT
     2701 ATGATGGAAC GGGGCCCCCA GACTCTGAAG GAAACATCCT TTAATCAGGC CTATGGTCGA
     2761 GATTTAATGG AGGCCCAAGA GTGGTGCAGG AAGTACATGA AATCAGGGAA TGTCAAGGAC
     2821 CTCCTCCAAG CCTGGGACCT CTATTATCAT GTGTTCCGAC GAATCTCAAA GTAAgcggcc
     2881 gccatatgca tcctaggcct attaatattc cggagtatac gtagccggct aacaaccggt
     2941 acctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
     3001 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3061 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3181 tcttatcatg tctggatcaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4021 ttgaagtggt ggcctaacta cggctacagt agaaggacag tatttggtat ctgcgctctg
     4081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5341 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5401 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5461 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5521 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ttggcttaac
     5581 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5641 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     5701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     5761 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     5821 acggccagtg ccaagttggg atctttgcat tggcccacgg ctctcaggat ggggatgctc
     5881 cccttcagca cccggttccc cttggaaact gatggtcctg gctctgtggc atggcagtgg
     5941 cactgtgagg agcccctacc agcagcacac agtgggtttg gcactgccac gctccggatg
     6001 ccgcgctctg atccaacccc ataatcaagg gaacccgaat tgccccatca ttgcccccac
     6061 cacccccatc ctgccgggcc ctcacacccc acgctgcctt gtggtgacat tccccagccc
     6121 aaacccacgg cttcatggct accgcggggc atttcccatt gccgccccat tatcagctct
     6181 gcacacctcc cgctgtaccc atgcctcgtg gctgcccttc tttgacgtat aatcttctaa
     6241 ttaatacccg gccttgtcaa agtggagcac aaacgttaat taattcccca gcaggcaggt
     6301 aattaacagt gtgactccct ttttgctgcg agtggggctg atacagagag atgtggcact
     6361 atggagccca cggggtcctg gcactgggtg cccacggagg tccccatgtg ctgcagtgtc
     6421 accgcctccg aggtgacagt attgtccctg cggtgtccct gcagctcagc tctgtccaca
     6481 gggccacctc cagtttggag gggacacaat gcagccccga tgcaacccat cctcgcagca
     6541 tcccagggac aaagacccca ctgcaagacc gcacacaggg ctgggtcccg ctcccctaat
     6601 atctacagtg cttttgcatg gccccttaat caatgcagtt aatcagcatg cgctcatgca
     6661 ccgctctgga gctgcaaagc ccctcgcagc gctgctcacc aacaccgcgc accgccccgg
     6721 cccagcctgc agcacgcgct gcaaacagga aagaaacaaa atattgccca aatgtaggca
     6781 aaggcattcg gctgccttga cctccgccgg gccgggccct gcctgactca gctccttact
     6841 cagcgctcgc ttcctccctc cggctgccac cgccgcagcg cacaccctga caaagagtgg
     6901 cccttaacgg gctctgaggt gcacccagca gtgcactcag cagtccaagg gccggcctgg
     6961 aggtttgcac cgctacgtgc tgacattagc attgaacttg gccctgggta gtgctgcagg
     7021 ccgggcgggg tgggtgtaga gagtgcagcg cgcgttgcac ccggtgcccc ttcccctccc
     7081 ttgcatccca gcaggctgca ccccagcacc aggcccgtgc atgcatgctc ctggtgttat
     7141 tgcagcctgg tgcatgcatg cgtcttagtg gtgcagcgct gtgcatgcat cctccttggt
     7201 gtgtagcagc ttagtgcatg catacccctc ggtgttattg ctgctctgtg caggcacgct
     7261 cattgtatca cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac
     7321 gcacactcat tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc
     7381 ggtgcgtgca tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg
     7441 ctatccctgc ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc
     7501 acctcggggg tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca
     7561 ctgtggaatc aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac
     7621 cctcacctcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     7681 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     7741 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     7801 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     7861 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     7921 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     7981 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     8041 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     8101 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     8161 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     8221 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     8281 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     8341 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     8401 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     8461 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     8521 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     8581 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     8641 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     8701 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     8761 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     8821 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt
     8881 ctctgttcct ccgcagcccc caagctagcg ctaagcttgc cacc
//