LOCUS       Exported                6483 bp DNA     circular SYN 15-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Broad Institute
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6483
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     repeat_region   1..130
                     /label=L-ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    151..814
                     /label=WDP0003
     promoter        844..892
                     /label=pB-Globin
     intron          977..1109
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    1131..1136
                     /label=Kozak
     CDS             1137..2066
                     /codon_start=1
                     /label=ChR2
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAVP"
     CDS             2076..2783
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    2829..3417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     polyA_signal    3586..3667
                     /label=SV40pA
                     /note="SV40 polyadenylation signal"
     repeat_region   3746..3886
                     /label=R-ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3961..4416
                     /direction=RIGHT
                     /label=F1 Origin
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4698..4802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4803..5663
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5834..6422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcgat agaccgctgt tgcagtattt cagggaacaa
      181 gatcagaatg gtatagccta cctgaaaaat aggcaatcct ttcaaaatta aatgcacttt
      241 ctcttgggta ggagacaaga taatctatga taagggaaga gagatgtctc cagcttttat
      301 tttttttctt gtaatttgca tttaagccct ggtaacagtt taatatgaag gtgactatgg
      361 cagctccatt tattcaatct atcaatactc tgttgtcaca ggaggagcca tgaggacttg
      421 agtgcagagg aggaatcccc acaaagcagt cttcactctt atcatagctt ccatgtgccc
      481 ttcttattca gcagctctca ttaaaggaac actatcttta gcacagggat tctacttgac
      541 attgacaagg acaggctcag tagcaaatgg gccaaaacta tgaataggcg attcacagca
      601 gagtaaaatc caatggcagc agacataaaa agaagctcaa aatgacaaac aagtcagaca
      661 aacacaaatt caagtaacaa taaaaaatgg agcactggtt tgactcgtac acaagcaaac
      721 agacacaaac aaaacaaaag agtcattaca cctggggcta aagacttctc ttatgtattg
      781 caaagtggaa ataagacctc caggcaaaat gtgaatcgat gtttaaactt cctgcagccc
      841 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctagcctgca
      901 agtcgaggag cgcagccttc cagaagcaga gcgcggcgcc ttaagctgca gaagttggtc
      961 gtgaggcact gggcacgtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     1021 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     1081 tgacatccac tttgcctttc tctccacagc gagctcgcta gcgcgatcgc gccaccatgg
     1141 actatggcgg cgctttgtct gccgtcggac gcgaactttt gttcgttact aatcctgtgg
     1201 tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta ctgtgccgga tggattgaat
     1261 ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct gcagtggctt gcagcaggat
     1321 tcagcatttt gctgctgatg ttctatgcct accaaacctg gaaatctaca tgcggctggg
     1381 aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat tctcgagttc ttttttgagt
     1441 ttaagaatcc ctctatgctc taccttgcca caggacaccg ggtgcagtgg ctgcgctatg
     1501 cagagtggct gctcacttgt cctgtcatcc ttatccgcct gagcaacctc accggcctga
     1561 gcaacgacta cagcaggaga accatgggac tccttgtctc agacatcggg actatcgtgt
     1621 ggggggctac cagcgccatg gcaaccggct atgttaaagt catcttcttt tgtcttggat
     1681 tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc atatatcgag ggttatcata
     1741 ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat ggcatggctg tttttcgtga
     1801 gctggggtat gttcccaatt ctcttcattt tggggcccga aggttttggc gtcctgagcg
     1861 tctatggctc caccgtaggt cacacgatta ttgatctgat gagtaaaaat tgttgggggt
     1921 tgttgggaca ctacctgcgc gtcctgatcc acgagcacat attgattcac ggagatatcc
     1981 gcaaaaccac caaactgaac atcggcggaa cggagatcga ggtcgagact ctcgtcgaag
     2041 acgaagccga ggccggagcc gtgccagcgg ccgccgtgag caagggcgag gaggataaca
     2101 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     2161 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2221 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2281 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     2341 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     2401 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     2461 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2521 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2581 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2641 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2701 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2761 gcatggacga gctgtacaag taaggcgcgc cccacccctg cagggaattc gatatcaagc
     2821 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3241 ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3361 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3421 gataccgagc gctgctcgag agatctgtga tagcggccat caagctggcc gcgactctag
     3481 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3541 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcagctta tcgataccgc
     3721 atgcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     3781 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3841 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     3901 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     3961 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4021 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4081 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4141 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4261 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     4321 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     4381 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     4441 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4501 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4561 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4621 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6481 tgt
//
