LOCUS       Exported               11802 bp DNA     circular SYN 18-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 18, 2023 from SnapGene Viewer 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        8..316
                     /locus_tag="Pbt1311"
                     /label=Pbt1311
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LacIq Casette PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     CDS             317..1408
                     /locus_tag="lacIq"
                     /label=lacIq
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LacIq Casette PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    1285..1290
                     /locus_tag="killed BsmBI site"
                     /label=killed BsmBI site
     terminator      1415..1475
                     /locus_tag="L3S2P21 Terminator"
                     /label=L3S2P21 Terminator
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LacIq Casette PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     CDS             complement(1629..2909)
                     /locus_tag="NBU2 integrase"
                     /label=NBU2 integrase
                     /note="NBU2 integrase"
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_recomb     3069..3091
                     /locus_tag="attN2"
                     /label=attN2
                     /note="attN2"
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             complement(3349..4083)
                     /locus_tag="ermG"
                     /label=ermG
                     /note="ermG"
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             4571..5432
                     /locus_tag="beta-lactamase from pBR322"
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    5362..5381
                     /locus_tag="AmpStop fw"
                     /label=AmpStop fw
     misc_recomb     5470..6255
                     /locus_tag="RP4 oriT/RSK oriV"
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
     promoter        6256..6334
                     /locus_tag="PcfxA"
                     /label=PcfxA
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    6257
                     /locus_tag="T missing"
                     /label=T missing
     misc_feature    6299..6302
                     /locus_tag="-33"
                     /label=-33
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     protein_bind    6303..6322
                     /locus_tag="LacO1'"
                     /label=LacO1'
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    6323..6330
                     /locus_tag="-7"
                     /label=-7
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    6335
                     /locus_tag="1"
                     /label=1
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     protein_bind    6336..6356
                     /locus_tag="LacO1"
                     /label=LacO1
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_difference 6361..6365
                     /locus_tag="RBS?"
                     /label=RBS?
                     /note="/Transferred_From=pNBU2-PcfxALacO23-Nanoluc-LacIq 
                     PCR
                     Product"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pNBU2-PcfxA-sfGFP PCR Product"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    6399..6406
                     /locus_tag="AscI - insertion of nuclease"
                     /label=AscI - insertion of nuclease
     misc_feature    6407..10204
                     /locus_tag="Pb2Cas12a e coli codon opt"
                     /label=Pb2Cas12a e coli codon opt
     misc_feature    8018..8023
                     /locus_tag="killed BsmBI"
                     /label=killed BsmBI
     misc_feature    9029..9031
                     /locus_tag="D875A (like FnCas12a D917A)"
                     /label=D875A (like FnCas12a D917A)
     misc_feature    10205..10270
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     misc_feature    10297..10326
                     /locus_tag="tL17 - terminator for nuclease"
                     /label=tL17 - terminator for nuclease
     misc_feature    10383..10426
                     /locus_tag="P1 promoter"
                     /label=P1 promoter
     misc_feature    10449..10467
                     /locus_tag="AWO-575 fw"
                     /label=AWO-575 fw
     misc_feature    10466..10469
                     /locus_tag="5prime junction"
                     /label=5prime junction
     misc_feature    10477..10606
                     /locus_tag="BBa_J72163 GlpT Promoter"
                     /label=BBa_J72163 GlpT Promoter
     misc_feature    10607..10642
                     /locus_tag="RBS"
                     /label=RBS
     misc_feature    10643..11359
                     /locus_tag="sfGFP"
                     /label=sfGFP
     misc_feature    11360..11488
                     /locus_tag="BBa_B0015 terminator"
                     /label=BBa_B0015 terminator
     misc_feature    11496..11499
                     /locus_tag="3prime junction"
                     /label=3prime junction
     misc_feature    11500..11535
                     /locus_tag="native Francisiella terminal repeat"
                     /label=native Francisiella terminal repeat
     misc_feature    11544..11562
                     /locus_tag="AWO-576 rev"
                     /label=AWO-576 rev
     misc_feature    11576..11688
                     /locus_tag="gRNA terminator"
                     /label=gRNA terminator
ORIGIN
        1 tcagccttga tctggaagaa gcaatgaaag ctgctgttaa gtctccgaat caggtattgt
       61 tcctgacagg tgtattccca tccggtaaac gcggatactt tgcagttgat ctgactcagg
      121 aataaattat aaattaaggt aagaagattg taggataagc taatgaaata gaaaaaggat
      181 gccgtcacac aacttgtcgg cattcttttt tgttttatta gttgaaaata tagtgaaaaa
      241 gttgcctaaa tatgtatgtt aacaaattat ttgtcgtaac tttgcactcc aaatctgttt
      301 ttaacatatg gcactagtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      361 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      421 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      481 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      541 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      601 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      661 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      721 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      781 ccagacaccc atcaacagta ttattttctc ccatgaggac ggtacgcgac tgggcgtgga
      841 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
      901 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
      961 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     1021 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     1081 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     1141 atacgacgat accgaggaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     1201 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1261 ggtgaagggc aatcagctgt tgcccgtatc actggtgaaa agaaaaacca ccctggcgcc
     1321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1381 ggtttcccga ctggaaagcg ggcagtgagc tttcctcggt accaaattcc agaaaagagg
     1441 cctcccgaaa ggggggcctt ttttcgtttt ggtcctactt gtgcctgttc tatttccgaa
     1501 ccgaccgctt gtatgaatcc atcaaaattc gttttctcta tgttggattc cttgttgctc
     1561 atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga aactgtttga
     1621 tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc cgctatgtca
     1681 atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac cacctgtgcc
     1741 gggattccga gagccagcgc attgcagatg aatgtccttc ttcctgcatg ggtactgagc
     1801 aaagcgtatt tgggtgtgac ttcatcaata cgttcatttc ccttgtagta ggtttcccgt
     1861 acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt catcttctgg
     1921 ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta tttgtccagt
     1981 atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt gactgtggtt
     2041 atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc cgaataccgc
     2101 aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta ttgcttatcc
     2161 ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa gattactttt
     2221 ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg atgatgtcct
     2281 ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc gatggtgctg
     2341 tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc aaactcgttg
     2401 aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac tgctgcaaat
     2461 ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac aaactcatcg
     2521 aacacctccc aaaagctgac aggggcttct tccggctgtt cttcgctggt gtctttcatt
     2581 ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg cacctcaaat
     2641 tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt gatttcggct
     2701 gcactttgct ttagcttgtt ggtacatccg ctctttaccc gctgcttatc tgcatcccat
     2761 ttggctacgt caatccggta gcccgttgta aactcgatgc gttggctggc aaagatgaca
     2821 cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg gctctccaat
     2881 gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc tacacccaaa
     2941 tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt tactctatat
     3001 tgtaatttac acttgattat cagtcgtttg cagtcttatg atattctgtg aaagtataag
     3061 ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa aacaaacacc
     3121 ctgttttaca cccaagaatg taaagtcggc tgtttttgtt ttatttaaga taatacaacc
     3181 actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac taatatttat
     3241 ctatccattc agtttgattt ttcaggactt tacatcgtcc tgaaagtatt tgttggtacc
     3301 ggtaccgagg acgcgtaaac atttacagtt gcatgtggcc tattgttttt agccgttaaa
     3361 tattttataa ctattaaata gcgatacaaa ttgttcgaaa ctaatattgt ttatatcata
     3421 tattctcgca tgttttaaag ctttattaaa ttgatttttt gtaaacagtt tttcgtactc
     3481 tttgttaacc catttcatta caaaagtttc atattttttt ctctctttaa atgccatttt
     3541 tgctggcttt ctttttaata caattaatgt gctatccact ttaggttttg gatggaaata
     3601 atacctagga atttttgcta atatagaaat atctacctct gccattaaca gcaatgctag
     3661 tgatctgttt gtatctaata acattttagc aaaaccatat tccactatta aataacttat
     3721 tgtggctgaa ctttcaaaaa caatttttcg aattatattt gtgcttatgt tgtaaggtat
     3781 gctgccaaat attttatatg gattgtggct aggaaatgta aatttcagta tatcatcatt
     3841 tactatttga tagttaggat aatttaagag cttattacga gttacctcac ataatttaga
     3901 atcaatttct atcgccgtta caaaattaca tctctttacc aatccagcag taaaatgacc
     3961 tttccctgca cctatttcaa agatgttatc tttttcatct aaacttatgc aattcattat
     4021 tttttctatg tgatattttg aagtaataaa attttgacta tcttttatat ttactttgtt
     4081 cattataacc tctccttaat ttattgcatc tcttttcgaa tatttatgtt ttttgagaaa
     4141 agaacgtact catggttcat cccgatatgc gtatcggtct gtatatcagc aactttctat
     4201 gtgtttcaac tacaatagtc atctattctc atctttctga gtccaccccc tgcaaagccc
     4261 ctctttacga cataaaaatt cggtcggaaa aggtatgcaa aagatgtttc tctctttaag
     4321 agaaactctt cgggatgcaa aaatatgaaa ataactccaa ttcaccaaat tatatagcga
     4381 cttttttaca aaatgctaaa atttgttgat ttccgtcaag caattgttga gcaaaaatgt
     4441 cttttacgat aaaatgatac ctcaatatca actgtttagc aaaacgatat ttctcttaaa
     4501 gagagaaaca cctttttgtt caccaatccc cgacttttaa tcccgcggcc atgattgaaa
     4561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact cataacgcgt
     5461 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
     5521 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     5581 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     5641 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     5701 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     5761 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
     5821 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
     5881 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
     5941 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
     6001 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
     6061 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
     6121 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
     6181 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
     6241 cctccaccgc ggtggttaca aagaaaattc gacaaactgt tatttttcta tctatttatt
     6301 tgaattgtga gcggataaca attacctttg tcggcaattg tgagcggata acaattaaat
     6361 aaagatattc tcgtcaaaca aatataaata atataaacgg cgcgccatgg ccccaaagaa
     6421 gaaacggaag gttatgcaaa taaacaacct caagatcata tatatgaagt tcacagattt
     6481 caccggcctg tatagcttgt ccaagactct caggttcgag ttgaagccga tcgggaagac
     6541 actcgaaaac ataaaaaagg cgggactttt ggagcaagat cagcataggg cggactccta
     6601 caagaaggtg aagaaaatca ttgatgaata tcacaaggct tttatagaaa agtcgctttc
     6661 taatttcgaa ctcaaatacc aatcggaaga caagctcgac tcgttggaag aatatctgat
     6721 gtactactcg atgaagagaa ttgaaaagac cgaaaaagat aagttcgcca aaatccagga
     6781 caacctcaga aaacagatcg cggaccacct taaaggcgat gagtcttaca aaacaatatt
     6841 ttccaaggat ttgatcagga agaatttgcc ggacttcgtc aagagcgacg aggaacggac
     6901 tttgattaag gagtttaagg acttcaccac gtactttaaa gggttctacg aaaatagaga
     6961 gaatatgtac agcgcggagg ataaaagcac cgccatctcc catagaatca tccacgagaa
     7021 tctccctaag tttgtggata acataaacgc gttctccaaa ataatcctca tcccagagct
     7081 gcgggagaag ctcaatcaga tataccagga cttcgaagag tacctgaacg tggagtcaat
     7141 tgatgaaata ttccatcttg actacttcag catggttatg acacaaaagc agatcgaagt
     7201 ctataacgcc attattggcg ggaagtctac caacgacaag aagatacagg ggcttaatga
     7261 gtatattaat ctgtataacc aaaagcacaa agattgcaag ttgccaaagc tgaaactgtt
     7321 gttcaagcag attcttagcg accggatagc gatctcttgg ctcccggata acttcaagga
     7381 tgatcaggag gcgcttgact caatagatac atgctacaag aatctcctga atgacgggaa
     7441 cgtgcttggg gaaggaaatc tgaagctgct gctcgagaac atagatacat acaatctgaa
     7501 gggaattttt attagaaatg atctccaact gacagatatt tcacagaaga tgtacgcttc
     7561 ttggaatgtc attcaagatg cagttattct tgatttgaaa aagcaggttt cccgcaagaa
     7621 gaaggagagc gcggaagatt acaacgatag actgaagaag ctctatacga gccaggagag
     7681 cttttcgata cagtacctca acgactgctt gagggcgtac gggaaaacag aaaacattca
     7741 agattacttt gcgaaactgg gggcggtcaa caacgagcac gagcagacga tcaacttgtt
     7801 tgcacaagtt aggaacgcct atacttccgt tcaagccatc ctcacaacac cgtaccctga
     7861 gaatgccaac ttggctcagg acaaagagac cgtcgcactt atcaagaacc tccttgattc
     7921 actgaagcgc cttcaaaggt ttatcaaacc tctcctcggc aagggcgatg agagcgacaa
     7981 ggacgagcgc ttctacggag acttcactcc cctttgggaa acgctcaacc aaataacccc
     8041 attgtataat atggtgagaa actacatgac aaggaaacct tattcgcagg agaaaataaa
     8101 gctcaatttt gaaaactcta ccttgcttgg gggatgggat ctgaacaaag agcacgataa
     8161 tactgcaatc atacttcgca agaatggtct ttactacctg gctatcatga aaaaaagcgc
     8221 caacaagatc ttcgataagg acaaattgga caactctggc gactgttacg aaaaaatggt
     8281 gtataaactg ctcccaggcg ccaacaaaat gcttcccaag gttttctttt ccaagtcgcg
     8341 catcgacgag ttcaaacctt ctgagaacat aatcgagaac tacaagaagg gaactcacaa
     8401 aaaaggggcc aacttcaatc tcgcagattg ccacaacttg atcgatttct ttaaatcctc
     8461 tatatcgaag catgaagact ggtccaagtt caactttcat ttcagcgaca cctcatctta
     8521 tgaggacctt tcagacttct acagagaggt ggagcaacaa gggtactcaa tctcattttg
     8581 cgatgtcagc gtggagtaca ttaacaagat ggttgagaag ggggaccttt acctcttcca
     8641 aatctacaat aaggatttct cggaattcag caagggcacg cctaatatgc acacgctcta
     8701 ttggaacagc ctcttctcga aagagaacct gaataatata atatacaaac tcaatggcca
     8761 agctgagatt ttcttcagga agaagtcact caattacaag cggccgacac acccggcgca
     8821 tcaagctatt aagaacaaga acaagtgtaa tgagaaaaaa gaatccatct tcgattatga
     8881 cctcgtcaag gataagcgct acaccgttga taaatttcag tttcacgttc caattaccat
     8941 gaactttaag tctacaggaa ataccaatat aaaccaacag gtcattgact atcttcgcac
     9001 ggaggacgat acgcacatca tcggaatcgc tcggggggag cggcacctgc tttatttggt
     9061 ggttatcgac tcccatggga aaatcgtgga acagttcacc ctcaatgaga ttgtgaacga
     9121 gtacggcgga aacatttata gaacgaatta ccacgacttg ctggacacga gagagcaaaa
     9181 cagggagaaa gcgagggagt catggcagac aatcgagaac ataaaagaat tgaaagaggg
     9241 ctatatctct caagtgattc ataaaataac ggatctcatg caaaagtacc atgcagtcgt
     9301 cgtgctcgag gatctcaaca tgggattcat gaggggaagg cagaaggtgg agaagcaggt
     9361 ctatcaaaaa tttgaagaaa tgctgatcaa taaacttaat tatctggtca acaaaaaggc
     9421 tgatcagaat agcgctggag ggctgttgca cgcctaccag ctgacatcaa agttcgagtc
     9481 cttccagaaa cttggcaagc agtctggttt tcttttttac attccagcct ggaatacctc
     9541 caaaattgat cctgtcaccg gatttgtgaa cttgtttgac accaggtacg agagcataga
     9601 caaggcgaag gcattctttg gaaagttcga ttccataagg tataatgcgg acaaagactg
     9661 gttcgagttt gcgttcgact ataacaactt cacaactaaa gcagagggga ctagaacaaa
     9721 ttggacgatc tgtacatacg gttcaaggat caggaccttc aggaatcaag cgaagaattc
     9781 tcaatgggac aacgaggaga tcgatctgac aaaagcctac aaggcttttt tcgccaaaca
     9841 tggaatcaat atctatgata atatcaagga ggccatagcc atggaaactg agaaaagctt
     9901 ctttgaggac ctgcttcatc tgcttaaatt gactctgcag atgcggaact ccattaccgg
     9961 cacaaccact gattatctca tttcgccagt ccatgactct aaaggaaact tctacgatag
    10021 caggatctgt gataatagcc ttcctgccaa cgcggacgcg aacggcgcgt ataatattgc
    10081 ccgcaaagga ttgatgctca tacagcaaat caaggattcg acgagctcga accgctttaa
    10141 gttctcacct attacgaata aagattggct catttttgca caagagaagc cgtacttgaa
    10201 tgacgactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
    10261 cgatgacaag taagcggccg cattaatgcg gctgcaattt ttttgggcgg ggccgcccaa
    10321 aaaaatccta gcaccctgca gcagtactgc ttgaccataa gaacaaaaaa acttccctta
    10381 aggataaagt ttggaagata aagctaaaag ttcttatctt tgcagtgcta gcgaattcga
    10441 gctcggtacc aagacgtcaa ctcgaccctc gagacggaaa gtgaaacgtg atttcatgcg
    10501 tcattttgaa cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt
    10561 atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttcggat cttagctact
    10621 agagaaagag gagaaatact agatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
    10681 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
    10741 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
    10801 gccggttcct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
    10861 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
    10921 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
    10981 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagagga
    11041 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
    11101 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
    11161 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
    11221 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
    11281 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
    11341 ggatgaactg tacaaatgac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
    11401 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
    11461 ccttcgggtg ggcctttctg cgtttatacg tctctaacgg tctaagaact ttaaataatt
    11521 tgtctgtata ttattaagct tggctgtttt ggcggatgag agaagatttt cagccccggc
    11581 ttatcggtca gtttcacctg atttacgtaa aaacccgctt cggcgggttt ttgcttttgg
    11641 aggggcagaa agatgaatga ctgtccacga cgctataccc aaaagaaatc tagatgatga
    11701 ttaaggatct tgcgtacaat ttgacgatcg tttcacgcta agatgctggc taattgccta
    11761 tcttccagtg atggaacagc atttgtgcat tggctgcaac aa
//
