LOCUS       pEM8315        3239 bp DNA     circular SYN 22-JAN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     Ligation of : pEM2179_pKH70_PrpsM-eGFP-LVA.xdna [2497  nt] : 
            (NotI[1261] / EcoRI[455]) to 
            PCR(pOSIP-KO-Insert-pfliC-mCerulean-prpsM-mCherry-NEBBuilder_Frag1.x
             dna) [734  nt] : (EcoRI[6] / NotI[743])
FEATURES             Location/Qualifiers
     source          1..3239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..455
                     /label=PrpsM
                     /note="note=""Fragment extracted from SalI-PrpsM-EcoRI PCR 
                     product.xdna [421  nt] : (SalI[9] / EcoRI[434])"
     CDS             478..1191
                     /label=mCherry2-L
     rep_origin      1317..2037
                     /direction=RIGHT
                     /label=ori29807
                     /note="vntifkey=""33"" - label=ori29807 -
                     ApEinfo_fwdcolor=pink - ApEinfo_revcolor=pink - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1758..2478"
     misc_feature    complement(2174..3034)
                     /label=AmpR
                     /note="vntifkey=""21"" - label=AmpR -
                     ApEinfo_fwdcolor=#0696ff - ApEinfo_revcolor=#0696ff - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - complement(2615..3475)"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtctctaaag gcgaggagaa taaccttgcc attatcaaag agttcatgcg ttttaaggta
      541 cacatggaag gtagcgttaa tggtcatgag ttcgaaattg aaggtgaggg agaaggacgg
      601 ccatacgagg gaacacagac cgcaaaatta aaagtgacta aagggggtcc gctgccgttc
      661 gcttgggaca ttttatcccc ccagtttatg tatggttcca aagcatacgt gaagcatcca
      721 gctgatattc cggattactt gaagttgagc tttcccgaag gattcaattg ggagcgtgtt
      781 atgaacttcg aagatggagg agtcgtcact gtcactcaag attccagtct gcaagacgga
      841 gagttcattt acaaggtcaa attacgcggc acaaactttc caagcgatgg tccggtaatg
      901 caatgccgca caatggggtg ggaagcttcg acggaacgga tgtacccaga ggacggtgct
      961 cttaagggtg aaatcaagca acggctgaag ctgaaagacg gtggtcacta cgacgcggaa
     1021 gtcaaaacaa catacaaagc taagaagccg gtacaacttc cgggggcgta caatgtggac
     1081 attaaacttg acattttaag tcataacgaa gattatacta ttgtggagca atatgaacgt
     1141 gccgagggac ggcactcgac gggaggaatg gacgagttat ataaatgata agcggccgct
     1201 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1261 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggcgga
     1321 gatattccgc ttcctcgctc actgactcgc tccgctcggt cgttcggctg cggcgagcgg
     1381 taccggctta ctggcggggc ggaaatttcc tggaagatgc caggaagaaa cttaacaggg
     1441 aagcgataag gccacggcag agccgttttt ccataggctc cgcccccctg acaagcatca
     1501 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg
     1561 tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     1621 actccgctgt tatggccgcg tttatctcat tccacgcctg acactcggtt ccgggaaggc
     1681 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctg
     1741 ttccggtaac tatcgacttg agtccaagcc ggaaagacac gacaaagcgc cactggcagc
     1801 agccactggt aagagggtgt acaagagatt actgatgcag agttcttgaa gtcagagcca
     1861 aactacggct aaactggaag gacgaatttg gtggcagcgg tcttgtacac cctgttacca
     1921 cggttcagaa gttccccaac ttactgaacc ttcgaaaaac cacctcccca ggtggttttt
     1981 tcgttttcag agcaagagat tacgacgcaa cggaaaggat ctcaagaggt tcatcttact
     2041 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2101 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa
     2161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     3181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
