LOCUS       pEM10687        3263 bp DNA     circular SYN 22-JAN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     Ligation of : pEM2179_pKH70_PrpsM-eGFP-LVA.xdna [2497  nt] : 
            (NotI[1261] / EcoRI[455]) to PCR_mNeptune2.xdna [758  nt] : 
            (EcoRI[6] / NotI[767])
FEATURES             Location/Qualifiers
     source          1..3263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..455
                     /label=PrpsM
                     /note="note=""Fragment extracted from SalI-PrpsM-EcoRI PCR 
                     product.xdna [421  nt] : (SalI[9] / EcoRI[434])"
     CDS             478..1209
                     /label=mNeptune2
     rep_origin      1341..2061
                     /direction=RIGHT
                     /label=ori29807
                     /note="vntifkey=""33"" - label=ori29807 -
                     ApEinfo_fwdcolor=pink - ApEinfo_revcolor=pink - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1758..2478"
     misc_feature    complement(2198..3058)
                     /label=AmpR
                     /note="vntifkey=""21"" - label=AmpR -
                     ApEinfo_fwdcolor=#0696ff - ApEinfo_revcolor=#0696ff - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - complement(2615..3475)"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gtgagcaaag gcgaagaact gattaaagaa aacatgcata tgaaactgta tatggaaggc
      541 accgtgaaca accatcattt taaatgcacc agcgaaggcg aaggcaaacc gtatgaaggc
      601 acccagaccg gccgcattaa agtggtggaa ggcggcccgc tgccgtttgc gtttgatatt
      661 ctggcgacct gctttatgta tggcagcaaa acctttatta accataccca gggcattccg
      721 gattttttta aacagagctt tccggaaggc tttacctggg aacgcgtgac cacctatgaa
      781 gatggcggcg tgctgaccgt gacccaggat accagcctgc aggatggctg cctgatttat
      841 aacgtgaaac tgcgcggcgt gaactttccg agcaacggcc cggtgatgca gaaaaaaacc
      901 ctgggctggg aagcgagcac cgaaaccctg tatccggcgg atggcggcct ggaaggccgc
      961 tgcgatatgg cgctgaaact ggtgggcggc ggccatctgc attgcaacct gaaaaccacc
     1021 tatcgcagca aaaaaccggc gaaaaacctg aaaatgccgg gcgtgtattt tgtggatcgc
     1081 cgcctggaac gcattaaaga agcggataac gaaacctatg tggaacagca tgaagtggcg
     1141 gtggcgcgct attgcgatct gccgagcaaa ctgggccata aactgaacgg catggatgaa
     1201 ctgtataaat aatgagcggc cgctctagag gcatcaaata aaacgaaagg ctcagtcgaa
     1261 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1321 tccgccgccc tagacctagg cggagatatt ccgcttcctc gctcactgac tcgctccgct
     1381 cggtcgttcg gctgcggcga gcggtaccgg cttactggcg gggcggaaat ttcctggaag
     1441 atgccaggaa gaaacttaac agggaagcga taaggccacg gcagagccgt ttttccatag
     1501 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     1561 gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg ctctcctgtt
     1621 cctgcctttc ggtttaccgg tgtcactccg ctgttatggc cgcgtttatc tcattccacg
     1681 cctgacactc ggttccggga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1741 cgttcagtcc gactgctgcg cctgttccgg taactatcga cttgagtcca agccggaaag
     1801 acacgacaaa gcgccactgg cagcagccac tggtaagagg gtgtacaaga gattactgat
     1861 gcagagttct tgaagtcaga gccaaactac ggctaaactg gaaggacgaa tttggtggca
     1921 gcggtcttgt acaccctgtt accacggttc agaagttccc caacttactg aaccttcgaa
     1981 aaaccacctc cccaggtggt tttttcgttt tcagagcaag agattacgac gcaacggaaa
     2041 ggatctcaag aggttcatct tactagtgct tggattctca ccaataaaaa acgcccggcg
     2101 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     2161 caggagtcca agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2221 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2281 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2341 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2401 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2461 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2521 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2581 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2641 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2701 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2761 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2821 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2881 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2941 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3001 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3061 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3121 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3181 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3241 tcacgaggcc ctttcgtctt cac
//
