LOCUS       pEM10689_        2849 bp DNA     circular SYN 22-JAN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     Ligation of : pEM2179_pKH70_PrpsM-eGFP-LVA.xdna [2497  nt] : 
            (NotI[1261] / EcoRI[455]) to PCR_miniSOG_gBlock.xdna [344  nt] : 
            (EcoRI[6] / NotI[353])
FEATURES             Location/Qualifiers
     source          1..2849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..455
                     /label=PrpsM
                     /note="note=""Fragment extracted from SalI-PrpsM-EcoRI PCR 
                     product.xdna [421  nt] : (SalI[9] / EcoRI[434])"
     CDS             478..798
                     /label=miniSOG
     rep_origin      927..1647
                     /direction=RIGHT
                     /label=ori29807
                     /note="vntifkey=""33"" - label=ori29807 -
                     ApEinfo_fwdcolor=pink - ApEinfo_revcolor=pink - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - 1758..2478"
     misc_feature    complement(1784..2644)
                     /label=AmpR
                     /note="vntifkey=""21"" - label=AmpR -
                     ApEinfo_fwdcolor=#0696ff - ApEinfo_revcolor=#0696ff - 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     width 5 offset 0 - complement(2615..3475)"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgaaag gctacggccg ttaattggtc
       61 gcctgagaag ttacggagag taaaaatgaa agttcgtgct tccgtcaaga aattatgccg
      121 taactgcaaa atcgttaagc gtgatggtgt catccgtgtg atttgcagtg ccgagccgaa
      181 gcataaacag cgccaaggct gattttttcg catatttttc ttgcaaagtt gggttgagct
      241 ggctagatta gccagccaat cttttgtatg tctgtacgtt tccatttgag tatcctgaaa
      301 acgggctttt cagcatggta cgtacatatt aaatagtagg agtgcatagt ggcccgtata
      361 gcaggcatta acattcctga tcagaaacac gccgtgatcg cgttaacttc gatctacggt
      421 gtcggcaaga cccgttctaa agccatcctg gctggaattc ataaggagga aaaacatatg
      481 gaaaaaagct ttgtgattac cgatccgcgc ctgccggata acccgattat ttttgcgagc
      541 gatggctttc tggaactgac cgaatatagc cgcgaagaaa ttctgggccg caacggccgc
      601 tttctgcagg gcccggaaac cgatcaggcg accgtgcaga aaattcgcga tgcgattcgc
      661 gatcagcgcg aaattaccgt gcagctgatt aactatacca aaagcggcaa aaaattttgg
      721 aacctgctgc atctgcagcc gatgcgcgat cagaaaggcg aactgcagta ttttattggc
      781 gtgcagctgg atggctaatg agcggccgct ctagaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      901 gacaaatccg ccgccctaga cctaggcgga gatattccgc ttcctcgctc actgactcgc
      961 tccgctcggt cgttcggctg cggcgagcgg taccggctta ctggcggggc ggaaatttcc
     1021 tggaagatgc caggaagaaa cttaacaggg aagcgataag gccacggcag agccgttttt
     1081 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     1141 aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct
     1201 cctgttcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat
     1261 tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact gtatgcacga
     1321 accccccgtt cagtccgact gctgcgcctg ttccggtaac tatcgacttg agtccaagcc
     1381 ggaaagacac gacaaagcgc cactggcagc agccactggt aagagggtgt acaagagatt
     1441 actgatgcag agttcttgaa gtcagagcca aactacggct aaactggaag gacgaatttg
     1501 gtggcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgaacc
     1561 ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat tacgacgcaa
     1621 cggaaaggat ctcaagaggt tcatcttact agtgcttgga ttctcaccaa taaaaaacgc
     1681 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1741 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     1801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2761 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2821 ggcgtatcac gaggcccttt cgtcttcac
//
