LOCUS       Exported                7520 bp ds-DNA     circular SYN 13-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 18, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pLOT_File_Version 2.0
            Plasmid Name: pAAVS1-(Puro)-TNNT2-Neo
            Note: Source vector: KW517
            ENZYME = BamHI
            ENZYME = PciI
            ENZMIX = [NotI+EcoRI],[GC'GGCC.GC,G'AATT.C]
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..789
                     /gene="AAVS1 5' Homology Arm"
                     /label=AAVS1 5' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=16744576
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    800..820
                     /gene="Splice Acceptor"
                     /label=Splice Acceptor
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=65280
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_signal     842..898
                     /gene="T2A-Like Spacer Peptide"
                     /label=T2A-Like Spacer Peptide
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=12632256
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     CDS             908..1504
                     /codon_start=1
                     /gene="Puro (Variant)"
                     /label=Puro (Variant)
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=33023
                     pLOT_fType=2
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    1529..1752
                     /gene="BGH polyA"
                     /label=BGH polyA
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8421631
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    1808..2412
                     /gene="TNNT2"
                     /label=TNNT2
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454016
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    2422..3216
                     /gene="Neo/Kan"
                     /label=Neo/Kan
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_Color=65535
                     pLOT_fType=2
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    3217..3236
                     /gene="MCS"
                     /label=MCS
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=16744448
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     polyA_signal    3246..3747
                     /gene="rb_glob_pa_terminator"
                     /label=rb_glob_pa_terminator
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8421631
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    3827..4646
                     /gene="AAVS1 3' Homology Arm"
                     /label=AAVS1 3' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=16744576
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     promoter        complement(4663..4682)
                     /gene="T7"
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=12816842
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=-1
                     pLOT_Thick=1"
     CDS             4777..4845
                     /codon_start=1
                     /gene="LacZ alpha"
                     /label=LacZ alpha
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=16744576
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      4847..5292
                     /gene="f1 Origin of Replication"
                     /label=f1 Origin of Replication
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=12632256
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     promoter        5363..5391
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454143
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    5433..5633
                     /gene="B-lactamase Secretion Signal"
                     /label=B-lactamase Secretion Signal
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454143
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     CDS             5631..6290
                     /codon_start=1
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Confers resistance to ampicillin
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=65535
                     pLOT_fType=2
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     misc_feature    6464..7052
                     /gene="pBR322 Ori"
                     /label=pBR322 Ori
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=12632256
                     pLOT_fType=1
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    6464..6492
                     /gene="RNAII Promoter"
                     /label=RNAII Promoter
                     /label=misc
                     /note="pUC Rep Ori RNAII Promoter
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454016
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    6500..7052
                     /gene="pBR322 Ori RNAII transcript"
                     /label=pBR322 Ori RNAII transcript
                     /note="pBR322 Ori RNAII transcript
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=0
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    complement(6503..6619)
                     /gene="pBR322 Ori RNAI transcript"
                     /label=pBR322 Ori RNAI transcript
                     /note="pBR322 Ori RNAI transcript
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=0
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=-1
                     pLOT_Thick=1"
     misc_feature    6506..6538
                     /gene="Loop 1"
                     /label=Loop 1
                     /note="pUC Rep Ori Loop 1.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=255
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    6506..6531
                     /gene="RNAI Terminator"
                     /label=RNAI Terminator
                     /label=misc
                     /note="pUC Rep Ori RNAI transcription terminator
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=0
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    6541..6569
                     /gene="Loop 2"
                     /label=Loop 2
                     /note="pUC Rep Ori Loop 2.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=255
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     misc_feature    6572..6601
                     /gene="Loop 3"
                     /label=Loop 3
                     /note="pUC Rep Ori Loop 3.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=255
                     pLOT_fType=3
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     promoter        complement(6617..6646)
                     /gene="RNAI Promoter"
                     /label=RNAI Promoter
                     /note="pUC Rep Ori RNAI Promoter
                     pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454143
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=-1
                     pLOT_Thick=1"
     promoter        7376..7405
                     /gene="LacZ Promoter"
                     /label=LacZ Promoter
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=8454143
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     protein_bind    7410..7432
                     /gene="LacO"
                     /label=LacO
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=10280598
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
     promoter        7476..7495
                     /gene="T3"
                     /note="pLOT_fWidth=0.3
                     pLOT_Alpha=255
                     pLOT_rOffset=1
                     pLOT_tcolor=0
                     pLOT_Color=11531631
                     pLOT_fType=6
                     pLOT_ShowLabel=1
                     pLOT_FHide=0
                     pLOT_fOrient=1
                     pLOT_Thick=1"
ORIGIN
        1 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
       61 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
      121 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
      181 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
      241 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
      301 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
      361 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
      421 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
      481 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
      541 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
      601 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
      661 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
      721 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
      781 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag
      841 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
      901 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
      961 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     1021 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     1081 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     1141 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     1201 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1261 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1321 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1381 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1441 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1501 tgcctgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     1561 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1621 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     1681 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1741 gcatgctggg gatgcggtgg gctctatgga ctagcggtgg cggcctcgac attgattatt
     1801 gactagtgtc atggagaaga cccaccttgc agatgtcctc actggggctg gcagagccgg
     1861 caacctgccc aaggctgctc agtccattag gagccagtag cctggaagat gtctttaccc
     1921 ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc aagattctgg
     1981 tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct gctcccagct
     2041 ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag agggacagct
     2101 ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag cttatctgag
     2161 cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta gccttgtccc
     2221 tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc cctgccccac
     2281 ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc cagcccacat
     2341 gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga ctgagcagac
     2401 gcctccagga tcgcggccgc catgattgaa caagatggat tgcacgcagg ttctccggcc
     2461 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2521 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2581 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     2641 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2701 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2761 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2821 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2881 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2941 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3001 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3061 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3121 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3181 atcgccttct atcgccttct tgacgagttc ttctgacctc gaggaagggc gaattcactc
     3241 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     3301 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     3361 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     3421 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     3481 agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg
     3541 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     3601 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     3661 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     3721 ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca gcccaagctt
     3781 ggatccctcg agttaattaa atctagaagt cgacagtact aagctttgac agaaaagccc
     3841 catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt
     3901 aggcagattc cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca
     3961 gaacctctaa ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca
     4021 gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc
     4081 taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct
     4141 ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga
     4201 ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg
     4261 ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag
     4321 ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc
     4381 taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc
     4441 ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc
     4501 cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc
     4561 aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc
     4621 cctacagggg ttcctggctc tgctctagcg atcgccaatt cgccctatag tgagtcgtat
     4681 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4741 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4801 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5281 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5401 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5461 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5521 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5581 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5641 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5701 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5761 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5821 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5881 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5941 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6001 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6061 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6121 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6181 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6241 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6301 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6361 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6601 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7141 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7201 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7441 gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa
     7501 agctgtgctt tctctgacca
//
