LOCUS       Exported                7289 bp ds-DNA     circular SYN 13-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 18, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pLOT_File_Version 2.0
            Plasmid Name: pAAVS1-(Neo)-TNNT2-Zeo
            Note: ZeoR ORF ligated in at NotI and AbsI
            ENZYME = AbsI
            ENZYME = EcoRI
            ENZYME = MfeI
            ENZYME = NotI
            ENZYME = SpeI
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..789
                     /gene="AAVS1 5' Homology Arm"
                     /label=AAVS1 5' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_rOffset=1
                     pLOT_Color=16744576
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    800..820
                     /gene="Splice Acceptor"
                     /label=Splice Acceptor
                     /label=SA_GT_Group
                     /note="pLOT_Thick=0
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454016
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_signal     842..898
                     /gene="T2A-Like Spacer Peptide"
                     /label=T2A-Like Spacer Peptide
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=12632256
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     CDS             914..1708
                     /codon_start=1
                     /gene="Neo/Kan"
                     /label=Neo/Kan
                     /note="pLOT_Thick=1
                     pLOT_fType=2
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=65535
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    1718..1941
                     /gene="BGH polyA"
                     /label=BGH polyA
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8421631
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        1997..2601
                     /gene="TNNT2 Promoter"
                     /label=TNNT2 Promoter
                     /note="pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454016
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    2611..2985
                     /gene="ZeoR"
                     /label=ZeoR
                     /note="pLOT_Thick=1
                     pLOT_fType=2
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_rOffset=1
                     pLOT_Color=10485760
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    2986..3005
                     /gene="MCS"
                     /label=MCS
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=16744448
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     polyA_signal    3006..3527
                     /gene="rabbit beta-globin polyadenylation signal"
                     /label=rabbit beta-globin polyadenylation signal
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8421631
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    3596..4415
                     /gene="AAVS1 3' Homology Arm"
                     /label=AAVS1 3' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_rOffset=1
                     pLOT_Color=16744576
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        complement(4432..4451)
                     /gene="T7"
                     /note="pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=12816842
                     pLOT_Alpha=255
                     pLOT_fOrient=-1"
     CDS             4546..4614
                     /codon_start=1
                     /gene="LacZ alpha"
                     /label=LacZ alpha
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=16744576
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      4616..5061
                     /gene="f1 Origin of Replication"
                     /label=f1 Origin of Replication
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=12632256
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        5132..5160
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454143
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    5202..5402
                     /gene="B-lactamase Secretion Signal"
                     /label=B-lactamase Secretion Signal
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454143
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     CDS             5400..6059
                     /codon_start=1
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Confers resistance to ampicillin
                     pLOT_Thick=1
                     pLOT_fType=2
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=65535
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     misc_feature    6233..6821
                     /gene="pBR322 Ori"
                     /label=pBR322 Ori
                     /note="pLOT_Thick=1
                     pLOT_fType=1
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=12632256
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    6233..6261
                     /gene="RNAII Promoter"
                     /label=RNAII Promoter
                     /label=misc
                     /note="pUC Rep Ori RNAII Promoter
                     pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454016
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    6269..6821
                     /gene="pBR322 Ori RNAII transcript"
                     /label=pBR322 Ori RNAII transcript
                     /note="pBR322 Ori RNAII transcript
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=0
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    complement(6272..6388)
                     /gene="pBR322 Ori RNAI transcript"
                     /label=pBR322 Ori RNAI transcript
                     /note="pBR322 Ori RNAI transcript
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=0
                     pLOT_Alpha=255
                     pLOT_fOrient=-1"
     misc_feature    6275..6307
                     /gene="Loop 1"
                     /label=Loop 1
                     /note="pUC Rep Ori Loop 1.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=255
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    complement(6275..6300)
                     /gene="RNAI Terminator"
                     /label=RNAI Terminator
                     /label=misc
                     /note="pUC Rep Ori RNAI transcription terminator
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=0
                     pLOT_Alpha=255
                     pLOT_fOrient=-1"
     misc_feature    6310..6338
                     /gene="Loop 2"
                     /label=Loop 2
                     /note="pUC Rep Ori Loop 2.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=255
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     misc_feature    6341..6370
                     /gene="Loop 3"
                     /label=Loop 3
                     /note="pUC Rep Ori Loop 3.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Thick=1
                     pLOT_fType=3
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=255
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        complement(6386..6415)
                     /gene="RNAI Promoter"
                     /label=RNAI Promoter
                     /note="pUC Rep Ori RNAI Promoter
                     pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454143
                     pLOT_Alpha=255
                     pLOT_fOrient=-1"
     misc_feature    7109..7130
                     /gene="CAP"
                     /label=CAP
                     /note="LacZ Promoter CAP Binding Site
                     pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=16777088
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        7145..7174
                     /gene="LacZ Promoter"
                     /label=LacZ Promoter
                     /note="pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=8454143
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     protein_bind    7179..7201
                     /gene="LacO"
                     /label=LacO
                     /note="pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=10280598
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
     promoter        7245..7264
                     /gene="T3"
                     /note="pLOT_Thick=1
                     pLOT_fType=6
                     pLOT_FHide=0
                     pLOT_fWidth=0.3
                     pLOT_ShowLabel=1
                     pLOT_tcolor=0
                     pLOT_rOffset=1
                     pLOT_Color=11531631
                     pLOT_Alpha=255
                     pLOT_fOrient=1"
ORIGIN
        1 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
       61 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
      121 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
      181 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
      241 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
      301 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
      361 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
      421 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
      481 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
      541 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
      601 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
      661 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
      721 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
      781 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag
      841 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
      901 gctcgagggt accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
      961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     1021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     1081 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     1141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     1201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     1261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     1321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     1381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     1441 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     1501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     1561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     1621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     1681 ctatcgcctt cttgacgagt tcttctgagt ttaaacccgc tgatcagcct cgactgtgcc
     1741 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1801 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1861 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     1921 caatagcagg catgctgggg atgcggtggg ctctatggac tagcggtggc ggcctcgaca
     1981 ttgattattg actagtgtca tggagaagac ccaccttgca gatgtcctca ctggggctgg
     2041 cagagccggc aacctgccca aggctgctca gtccattagg agccagtagc ctggaagatg
     2101 tctttacccc cagcatcagt tcaagtggag cagcacataa ctcttgccct ctgccttcca
     2161 agattctggt gctgagactt atggagtgtc ttggaggttg ccttctgccc cccaaccctg
     2221 ctcccagctg gccctcccag gcctgggttg ctggcctctg ctttatcagg attctcaaga
     2281 gggacagctg gtttatgttg catgactgtt ccctgcatat ctgctctggt tttaaatagc
     2341 ttatctgagc agctggagga ccacatgggc ttatatggcg tggggtacat gttcctgtag
     2401 ccttgtccct ggcacctgcc aaaatagcag ccaacacccc ccacccccac cgccatcccc
     2461 ctgccccacc cgtcccctgt cgcacattcc tccctccgca gggctggctc accaggcccc
     2521 agcccacatg cctgcttaaa gccctctcca tcctctgcct cacccagtcc ccgctgagac
     2581 tgagcagacg cctccaggat cgcggccgcc atggccaagt tgaccagtgc cgttccggtg
     2641 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     2701 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     2761 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     2821 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     2881 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     2941 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacctcg aggcaattgg
     3001 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     3061 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     3121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     3181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     3241 atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa
     3301 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     3361 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     3421 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     3481 tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag
     3541 cccaagcttg gatccctcga gttaattaaa tctagaagtc gacagtacta agctttgaca
     3601 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     3661 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     3721 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     3781 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     3841 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     3901 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     3961 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     4021 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     4081 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     4141 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     4201 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     4261 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     4321 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     4381 tgccccttcc ctacaggggt tcctggctct gctctagcga tcgccaattc gccctatagt
     4441 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6361 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7081 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7141 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7201 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
     7261 gggaacaaaa gctgtgcttt ctctgacca
//
