LOCUS       Exported                6905 bp ds-DNA     circular SYN 13-JUL-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 18, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pLOT_File_Version 2.0
            Plasmid Name: pAAVS1-(Blast)-TNNT2-Zeo
            Note: pAAVS1-(Neo)-TNNT2-Zeo with BlastR ligated in the KpnI
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..789
                     /gene="AAVS1 5' Homology Arm"
                     /label=AAVS1 5' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_Alpha=255
                     pLOT_Color=16744576
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1"
     misc_feature    800..820
                     /gene="Splice Acceptor"
                     /label=Splice Acceptor
                     /label=SA_GT_Group
                     /note="pLOT_Alpha=255
                     pLOT_Color=8454016
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_signal     842..898
                     /gene="T2A-Like Spacer Peptide"
                     /label=T2A-Like Spacer Peptide
                     /note="pLOT_Alpha=255
                     pLOT_Color=12632256
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    914..1324
                     /gene="BlastR"
                     /label=BlastR
                     /note="pLOT_Alpha=255
                     pLOT_Color=33023
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=2
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1"
     misc_feature    1334..1557
                     /gene="BGH polyA"
                     /label=BGH polyA
                     /note="pLOT_Alpha=255
                     pLOT_Color=8421631
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     promoter        1613..2217
                     /gene="TNNT2 Promoter"
                     /label=TNNT2 Promoter
                     /note="pLOT_Alpha=255
                     pLOT_Color=8454016
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    2227..2601
                     /gene="ZeoR"
                     /label=ZeoR
                     /note="pLOT_Alpha=255
                     pLOT_Color=10485760
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=2
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1"
     misc_feature    2602..2621
                     /gene="MCS"
                     /label=MCS
                     /note="pLOT_Alpha=255
                     pLOT_Color=16744448
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     polyA_signal    2622..3143
                     /gene="rabbit beta-globin polyadenylation signal"
                     /label=rabbit beta-globin polyadenylation signal
                     /note="pLOT_Alpha=255
                     pLOT_Color=8421631
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    3212..4031
                     /gene="AAVS1 3' Homology Arm"
                     /label=AAVS1 3' Homology Arm
                     /note="Validated homology arm for targeted integration into
                     the AAVS1 safe harbor locus
                     pLOT_Alpha=255
                     pLOT_Color=16744576
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1"
     promoter        complement(4048..4067)
                     /gene="T7"
                     /label=T7 promoter
                     /label=T7
                     /note="pLOT_Alpha=255
                     pLOT_Color=12816842
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=-1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     CDS             4162..4230
                     /codon_start=1
                     /gene="LacZ alpha"
                     /label=LacZ alpha
                     /note="pLOT_Alpha=255
                     pLOT_Color=16744576
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     rep_origin      4232..4677
                     /gene="f1 Origin of Replication"
                     /label=f1 Origin of Replication
                     /note="pLOT_Alpha=255
                     pLOT_Color=12632256
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     promoter        4748..4776
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="pLOT_Alpha=255
                     pLOT_Color=8454143
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    4818..5018
                     /gene="B-lactamase Secretion Signal"
                     /label=B-lactamase Secretion Signal
                     /note="pLOT_Alpha=255
                     pLOT_Color=8454143
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     CDS             5016..5675
                     /codon_start=1
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Confers resistance to ampicillin
                     pLOT_Alpha=255
                     pLOT_Color=65535
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=2
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     misc_feature    5849..6437
                     /gene="pBR322 Ori"
                     /label=pBR322 Ori
                     /note="pLOT_Alpha=255
                     pLOT_Color=12632256
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=1
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    5849..5877
                     /gene="RNAII Promoter"
                     /label=RNAII Promoter
                     /label=misc
                     /note="pUC Rep Ori RNAII Promoter
                     pLOT_Alpha=255
                     pLOT_Color=8454016
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    5885..6437
                     /gene="pBR322 Ori RNAII transcript"
                     /label=pBR322 Ori RNAII transcript
                     /note="pBR322 Ori RNAII transcript
                     pLOT_Alpha=255
                     pLOT_Color=0
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    complement(5888..6004)
                     /gene="pBR322 Ori RNAI transcript"
                     /label=pBR322 Ori RNAI transcript
                     /note="pBR322 Ori RNAI transcript
                     pLOT_Alpha=255
                     pLOT_Color=0
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=-1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    5891..5923
                     /gene="Loop 1"
                     /label=Loop 1
                     /note="pUC Rep Ori Loop 1.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Alpha=255
                     pLOT_Color=255
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    complement(5891..5916)
                     /gene="RNAI Terminator"
                     /label=RNAI Terminator
                     /label=misc
                     /note="pUC Rep Ori RNAI transcription terminator
                     pLOT_Alpha=255
                     pLOT_Color=0
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=-1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    5926..5954
                     /gene="Loop 2"
                     /label=Loop 2
                     /note="pUC Rep Ori Loop 2.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Alpha=255
                     pLOT_Color=255
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    5957..5986
                     /gene="Loop 3"
                     /label=Loop 3
                     /note="pUC Rep Ori Loop 3.  Initiates RNAI/RNAII 
                     interaction.
                     pLOT_Alpha=255
                     pLOT_Color=255
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=3
                     pLOT_fWidth=1
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     promoter        complement(6002..6031)
                     /gene="RNAI Promoter"
                     /label=RNAI Promoter
                     /note="pUC Rep Ori RNAI Promoter
                     pLOT_Alpha=255
                     pLOT_Color=8454143
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=-1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     misc_feature    6725..6746
                     /gene="CAP"
                     /label=CAP
                     /note="LacZ Promoter CAP Binding Site
                     pLOT_Alpha=255
                     pLOT_Color=16777088
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     promoter        6761..6790
                     /gene="LacZ Promoter"
                     /label=LacZ Promoter
                     /note="pLOT_Alpha=255
                     pLOT_Color=8454143
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     protein_bind    6795..6817
                     /gene="LacO"
                     /label=LacO
                     /note="pLOT_Alpha=255
                     pLOT_Color=10280598
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
     promoter        6861..6880
                     /gene="T3"
                     /label=T3 promoter
                     /label=T3
                     /note="pLOT_Alpha=255
                     pLOT_Color=11531631
                     pLOT_FHide=0
                     pLOT_ShowLabel=1
                     pLOT_fOrient=1
                     pLOT_fType=6
                     pLOT_fWidth=0.3
                     pLOT_rOffset=1
                     pLOT_tcolor=0"
ORIGIN
        1 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
       61 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
      121 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
      181 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
      241 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
      301 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
      361 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
      421 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
      481 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
      541 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
      601 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
      661 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
      721 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
      781 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag
      841 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
      901 gctcgagggt accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
      961 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     1021 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     1081 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     1141 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     1201 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     1261 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc tgcagctgca
     1321 gtaggtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1561 ggtgggctct atggactagc ggtggcggcc tcgacattga ttattgacta gtgtcatgga
     1621 gaagacccac cttgcagatg tcctcactgg ggctggcaga gccggcaacc tgcccaaggc
     1681 tgctcagtcc attaggagcc agtagcctgg aagatgtctt tacccccagc atcagttcaa
     1741 gtggagcagc acataactct tgccctctgc cttccaagat tctggtgctg agacttatgg
     1801 agtgtcttgg aggttgcctt ctgcccccca accctgctcc cagctggccc tcccaggcct
     1861 gggttgctgg cctctgcttt atcaggattc tcaagaggga cagctggttt atgttgcatg
     1921 actgttccct gcatatctgc tctggtttta aatagcttat ctgagcagct ggaggaccac
     1981 atgggcttat atggcgtggg gtacatgttc ctgtagcctt gtccctggca cctgccaaaa
     2041 tagcagccaa caccccccac ccccaccgcc atccccctgc cccacccgtc ccctgtcgca
     2101 cattcctccc tccgcagggc tggctcacca ggccccagcc cacatgcctg cttaaagccc
     2161 tctccatcct ctgcctcacc cagtccccgc tgagactgag cagacgcctc caggatcgcg
     2221 gccgccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     2281 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     2341 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     2401 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     2461 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     2521 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     2581 gtggccgagg agcaggactg acctcgaggc aattggaatt cactcctcag gtgcaggctg
     2641 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     2701 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     2761 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     2821 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     2881 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     2941 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     3001 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     3061 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     3121 gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggatc cctcgagtta
     3181 attaaatcta gaagtcgaca gtactaagct ttgacagaaa agccccatcc ttaggcctcc
     3241 tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca gattccttat
     3301 ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc tctaaggttt
     3361 gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg tgggagggaa
     3421 gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc tctcccagaa
     3481 cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc tgcagcttcc
     3541 ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag ttggtcctga
     3601 gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct ccgtgcgtca
     3661 gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg tggtgaggag
     3721 gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt gttcaccagg
     3781 tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa gagtccccag
     3841 tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa ggccctgctc
     3901 tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct tccctgtccc
     3961 ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac aggggttcct
     4021 ggctctgctc tagcgatcgc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     4081 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4141 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4201 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4681 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4741 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4801 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4861 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4921 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4981 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5041 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5101 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5161 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5221 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5281 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5341 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5401 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5461 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5521 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5581 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5641 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6241 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6661 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6721 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6781 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6841 atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg tgctttctct
     6901 gacca
//
