LOCUS       Exported               13035 bp ds-DNA     circular SYN 19-DEC-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   sidhartm
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             327..986
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /note="color: #99ff33"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      1137..1804
                     /label=pUC Ori
                     /note="pUC Ori"
                     /note="color: #ffff99"
     primer_bind     1649..1668
                     /label=pBR322ori-FSEQ
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     misc_recomb     2208..3044
                     /label=HA-R
                     /note="HA-R"
                     /note="color: #dd0000"
     promoter        3074..3389
                     /note="TRE"
                     /note="color: #0000dd; direction: RIGHT"
     promoter        3075..3389
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    3083..3101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3119..3137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3154..3172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3190..3208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3226..3244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3261..3279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    3297..3315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_recomb     3406..3430
                     /label=AttB1
                     /note="AttB1"
                     /note="color: #000000"
     primer_bind     3465..3484
                     /label=T7fwSEQ
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     primer_bind     3465..3484
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        3465..3483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3533..3553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3554..7654
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /note="This feature has 3 segments:
                        1: 3554 .. 6067 / #993366
                        2: 6068 .. 6070 / #cc99b2
                        3: 6071 .. 7654 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9827..9847
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             9856..9873
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_recomb     9894..9918
                     /label=AttB2
                     /note="AttB2"
                     /note="color: #000000"
     primer_bind     9909..9928
                     /label=PolyA-F
                     /note="color: black; sequence: ACAAAGTGGTTGATCCGGGG"
     polyA_signal    9974..10167
                     /label=pA
                     /note="pA"
                     /note="color: #0099ff; direction: RIGHT"
     polyA_signal    complement(10168..10395)
                     /label=pA
                     /note="pA"
                     /note="color: #ffffcc; direction: LEFT"
     CDS             complement(10430..11455)
                     /codon_start=1
                     /label=Hygro
                     /note="Hygro"
                     /note="color: #999900"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    11465..11527
                     /label=2A
                     /note="2A"
                     /note="color: #ffff00"
     misc_feature    11543..11567
                     /label=SA
                     /note="SA"
                     /note="color: #33cc00"
     misc_recomb     11574..12377
                     /label=HA-L
                     /note="HA-L"
                     /note="color: #dd0000"
     primer_bind     complement(11710..11736)
                     /label=TEST4
                     /note="color: black; sequence: TTCTTCCTCCAACCCGGGCCCCTATGT"
     primer_bind     complement(12391..12410)
                     /label=T7fwSEQ
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     primer_bind     complement(12391..12410)
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     rep_origin      12595..12901
                     /direction=RIGHT
                     /label=f1(+) Ori
                     /note="f1(+) Ori"
                     /note="color: #ffff99"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctcaga gcagagccag
     2221 gaacccctgt agggaagggg caggagagcc aggggcatga gatggtggac gaggaagggg
     2281 gacagggaag cctgagcgcc tctcctgggc ttgccaagga ctcaaaccca gaagcccaga
     2341 gcagggcctt agggaagcgg gaccctgctc tgggcggagg aatatgtccc agatagcact
     2401 ggggactctt taaggaaaga aggatggaga aagagaaagg gagtagaggc ggccacgacc
     2461 tggtgaacac ctaggacgca ccattctcac aaagggagtt ttccacacgg acacccccct
     2521 cctcaccaca gccctgccag gacggggctg gctactggcc ttatctcaca ggtaaaactg
     2581 acgcacggag gaacaatata aattggggac tagaaaggtg aagagccaaa gttagaactc
     2641 aggaccaact tattctgatt ttgtttttcc aaactgcttc tcctcttggg aagtgtaagg
     2701 aagctgcagc accaggatca gtgaaacgca ccagacagcc gcgtcagagc agctcaggtt
     2761 ctgggagagg gtagcgcagg gtggccactg agaaccgggc aggtcacgca tccccccctt
     2821 ccctcccacc ccctgccaag ctctccctcc caggatcctc tctggctcca tcgtaagcaa
     2881 accttagagg ttctggcaag gagagagatg gctccaggaa atgggggtgt gtcaccagat
     2941 aaggaatctg cctaacagga ggtgggggtt agacccaata tcaggagact aggaaggagg
     3001 aggcctaagg atggggcttt tctgtcacca atcctgtccc tagtttaatt aaccctctag
     3061 agcttagtac tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     3121 cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt
     3181 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     3241 gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc
     3301 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca
     3361 gagctcgttt agtgaaccgt cagatcgcct ggagaattcc gatcaacaag tttgtacaaa
     3421 aaagcaggct ccgcggtttt caggttggac cggtgcgcgg ccgctaatac gactcactat
     3481 agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa
     3541 gaagcggaaa gtcgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
     3601 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     3661 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     3721 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     3781 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     3841 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     3901 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     3961 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     4021 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     4081 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     4141 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     4201 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     4261 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     4321 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     4381 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     4441 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     4501 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     4561 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     4621 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     4681 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     4741 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     4801 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     4861 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     4921 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     4981 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     5041 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     5101 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     5161 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     5221 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     5281 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     5341 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     5401 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     5461 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     5521 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     5581 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     5641 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     5701 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     5761 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     5821 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     5881 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     5941 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     6001 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     6061 tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     6121 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     6181 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     6241 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     6301 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     6361 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     6421 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     6481 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     6541 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     6601 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     6661 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     6721 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     6781 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     6841 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     6901 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     6961 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     7021 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     7081 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     7141 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     7201 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     7261 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     7321 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     7381 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     7441 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     7501 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     7561 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     7621 gacacggatc gacctgtctc agctgggagg tgactctgga ggatctagcg gaggatcctc
     7681 tggcagcgag acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag
     7741 cggcggcagc agcaccctaa atatagaaga tgagtatcgg ctacatgaga cctcaaaaga
     7801 gccagatgtt tctctagggt ccacatggct gtctgatttt cctcaggcct gggcggaaac
     7861 cgggggcatg ggactggcag ttcgccaagc tcctctgatc atacctctga aagcaacctc
     7921 tacccccgtg tccataaaac aataccccat gtcacaagaa gccagactgg ggatcaagcc
     7981 ccacatacag agactgttgg accagggaat actggtaccc tgccagtccc cctggaacac
     8041 gcccctgcta cccgttaaga aaccagggac taatgattat aggcctgtcc aggatctgag
     8101 agaagtcaac aagcgggtgg aagacatcca ccccaccgtg cccaaccctt acaacctctt
     8161 gagcgggctc ccaccgtccc accagtggta cactgtgctt gatttaaagg atgccttttt
     8221 ctgcctgaga ctccacccca ccagtcagcc tctcttcgcc tttgagtgga gagatccaga
     8281 gatgggaatc tcaggacaat tgacctggac cagactccca cagggtttca aaaacagtcc
     8341 caccctgttt aatgaggcac tgcacagaga cctagcagac ttccggatcc agcacccaga
     8401 cttgatcctg ctacagtacg tggatgactt actgctggcc gccacttctg agctagactg
     8461 ccaacaaggt actcgggccc tgttacaaac cctagggaac ctcgggtatc gggcctcggc
     8521 caagaaagcc caaatttgcc agaaacaggt caagtatctg gggtatcttc taaaagaggg
     8581 tcagagatgg ctgactgagg ccagaaaaga gactgtgatg gggcagccta ctccgaagac
     8641 ccctcgacaa ctaagggagt tcctagggaa ggcaggcttc tgtcgcctct tcatccctgg
     8701 gtttgcagaa atggcagccc ccctgtaccc tctcaccaaa ccggggactc tgtttaattg
     8761 gggcccagac caacaaaagg cctatcaaga aatcaagcaa gctcttctaa ctgccccagc
     8821 cctggggttg ccagatttga ctaagccctt tgaactcttt gtcgacgaga agcagggcta
     8881 cgccaaaggt gtcctaacgc aaaaactggg accttggcgt cggccggtgg cctacctgtc
     8941 caaaaagcta gacccagtag cagctgggtg gcccccttgc ctacggatgg tagcagccat
     9001 tgccgtactg acaaaggatg caggcaagct aaccatggga cagccactag tcattctggc
     9061 cccccatgca gtagaggcac tagtcaaaca accccccgac cgctggcttt ccaacgcccg
     9121 gatgactcac tatcaggcct tgcttttgga cacggaccgg gtccagttcg gaccggtggt
     9181 agccctgaac ccggctacgc tgctcccact gcctgaggaa gggctgcaac acaactgcct
     9241 tgatatcctg gccgaagccc acggaacccg acccgaccta acggaccagc cgctcccaga
     9301 cgccgaccac acctggtaca cggatggaag cagtctctta caagagggac agcgtaaggc
     9361 gggagctgcg gtgaccaccg agaccgaggt aatctgggct aaagccctgc cagccgggac
     9421 atccgctcag cgggctgaac tgatagcact cacccaggcc ctaaagatgg cagaaggtaa
     9481 gaagctaaat gtttatactg atagccgtta tgcttttgct actgcccata tccatggaga
     9541 aatatacaga aggcgtgggt ggctcacatc agaaggcaaa gagatcaaaa ataaagacga
     9601 gatcttggcc ctactaaaag ccctctttct gcccaaaaga cttagcataa tccattgtcc
     9661 aggacatcaa aagggacaca gcgccgaggc tagaggcaac cggatggctg accaagcggc
     9721 ccgaaaggca gccatcacag agactccaga cacctctacc ctcctcatag aaaattcatc
     9781 accctctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag
     9841 gaaagtctaa ccggtcatca tcaccatcac cattgagttt ggtgggcgcg ccgacccagc
     9901 tttcttgtac aaagtggttg atccggggat cctctagtca gcgtttatct gcagaattcg
     9961 cccttgttta aacgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    10021 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    10081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10141 ataaagcatt tttttcactg cctcgaccca tagagcccac cgcatcccca gcatgcctgc
    10201 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
    10261 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
    10321 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
    10381 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac tattcctttg
    10441 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
    10501 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
    10561 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
    10621 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
    10681 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
    10741 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
    10801 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
    10861 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
    10921 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
    10981 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
    11041 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
    11101 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
    11161 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
    11221 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
    11281 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
    11341 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
    11401 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatctgca
    11461 gcctagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctctc
    11521 cgctgccaga tctctcgagg ccctgtggga ggaagagaag aggtcaggga tctgccccac
    11581 tgtggggtgg aggggacaga taaaagtacc cagaaccaga gccacattaa ccggccctgg
    11641 gaatataagg tggtcccagc tcggggacac aggatccctg gaggcagcaa acatgctgtc
    11701 ctgaagtgga cataggggcc cgggttggag gaagaagact agctgagctc tcggacccct
    11761 ggaagatgcc atgacagggg gctggaagag ctagcacaga ctagagaggt aaggggggta
    11821 ggggagctgc ccaaatgaaa ggagtgagag gtgacccgaa tccacaggag aacggggtgt
    11881 ccaggcaaag aaagcaagag gatggagagg tggctaaagc cagggagacg gggtactttg
    11941 gggttgtcca gaaaaacggt gatgatgcag gcctacaaga aggggaggcg ggacgcaagg
    12001 gagacatccg tcggagaagg ccatcctaag aaacgagaga tggcacaggc cccagaagga
    12061 gaaggaaaag ggaacccagc gagtgaagac ggcatggggt tgggtgaggg aggagagatg
    12121 cccggagagg acccagacac ggggaggatc cgctcagagg acatcacgtg gtgcagcgcc
    12181 gagaaggaag tgctccggaa agagcatcct tgggcagcaa cacagcagag agcaagggga
    12241 agagggagtg gaggaagacg gaacctgaag gaggcggcag ggaaggatct gggccagccg
    12301 tagaggtgac ccaggccaca agctgcagac agaaagcggc acaggcccag gggagagaat
    12361 gctggtcaga gaaagcaggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
    12421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    12481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    12541 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    12601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    12661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    12721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    12781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    12841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    12901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    12961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    13021 acgcttacaa tttag
//
