LOCUS       Exported                4141 bp ds-DNA     circular SYN 18-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    332..361
                     /label=lacUV5 promoter
     gene            430..1995
                     /label=SAVED-CHAT
                     /note="product: 
                     THHADHDCQPHRESVQPDEIQCILVLDLSIDNAITACSVTPHLPRAARRVELHLNDFGA
                     ERAPYGGASDRRTWRCWMQAVDAMLADARAQLGAEVEFTHYYLAGRAALPVFAYLGLRL
                     GKQANITTVNRRDDGCWDVVPCQRPASSAASGVSPSARFFDEVRGLDTDERSSESGMVA
                     VWVSTQRDVDRGLLRAFARARGDRDLAGIVSLRARPAAGDDTGDMRLLEGADGPDAARE
                     LVNCFRSIPNQYPRSSGLMVFVSGPVTLAAMVGRAINPRIHGPVWWPYFRGGEYEPALE
                     YPWPLISGPPRILIATANAPEGENPTLDVEAELKHLEEALAEPRKRKLCEVQRCPAATV
                     SDITSALRSFKPHILHFIGHGTALGVYLRSAEHDGAQFVRGEDFQQMIATSLRQKDREM
                     HLVVLNACCTHELAKALTEQVSCTIGTDIEVYDSASIHFAARFYDHLVHGTSVHYAFNA
                     AVDECRAHSTSGQEVFCLHPAAERAPASATPPVRADELVFFSPAERHS"
     primer_bind     800..819
                     /label=BG28355
     primer_bind     1437..1456
                     /label=BG28356
     primer_bind     complement(1526..1548)
                     /label=BG29118
     primer_bind     complement(1671..1692)
                     /label=BG29120
     misc_feature    2018..2028
                     /label=MCS-2
                     /note="label: MCS-2"
     terminator      2052..2099
                     /label=T7 terminator
                     /note="label: T7 terminator"
     primer_bind     2270..2299
                     /label=BG12696
     primer_bind     2270..2291
                     /label=BG13247
     primer_bind     complement(2270..2290)
                     /label=BG17673
     primer_bind     complement(2271..2291)
                     /label=BG13276
     primer_bind     complement(2271..2291)
                     /label=BG15746
     primer_bind     complement(2271..2291)
                     /label=BG13272
     primer_bind     complement(2272..2291)
                     /label=BG12166
     primer_bind     complement(2276..2291)
                     /label=BG14660
     primer_bind     complement(2279..2299)
                     /label=BG10641
     primer_bind     complement(2284..2304)
                     /label=BG10640
     primer_bind     complement(2288..2308)
                     /label=BG26402
     primer_bind     complement(2288..2308)
                     /label=BG15429
     primer_bind     2289..2309
                     /label=BG10654
     primer_bind     2289..2309
                     /label=BG15090
     primer_bind     complement(2289..2309)
                     /label=BG10639
     primer_bind     2290..2310
                     /label=BG13277
     primer_bind     2290..2310
                     /label=BG14645
     primer_bind     2290..2310
                     /label=BG13273
     primer_bind     2290..2305
                     /label=BG14675
     primer_bind     2291..2311
                     /label=BG17674
     primer_bind     complement(2292..2327)
                     /label=BG12381
     primer_bind     2292..2315
                     /label=BG12172
     primer_bind     complement(2292..2315)
                     /label=BG11057
     primer_bind     2294..2314
                     /label=BG10653
     primer_bind     complement(2294..2314)
                     /label=BG10638
     primer_bind     complement(2298..2323)
                     /label=BG12170
     primer_bind     complement(2299..2319)
                     /label=BG10637
     primer_bind     complement(2301..2321)
                     /label=BG15427
     primer_bind     2302..2322
                     /label=BG15088
     primer_bind     complement(2304..2324)
                     /label=BG10636
     primer_bind     2308..2331
                     /label=BG26135
     primer_bind     complement(2308..2331)
                     /label=BG26166
     primer_bind     2309..2329
                     /label=BG10650
     primer_bind     complement(2309..2329)
                     /label=BG10635
     primer_bind     complement(2309..2329)
                     /label=BG15744
     primer_bind     complement(2309..2329)
                     /label=BG13268
     primer_bind     complement(2313..2333)
                     /label=BG16242
     primer_bind     2314..2334
                     /label=BG16240
     primer_bind     2314..2334
                     /label=BG13567
     primer_bind     2314..2334
                     /label=BG10649
     primer_bind     2314..2334
                     /label=BG24896
     primer_bind     complement(2314..2334)
                     /label=BG13566
     primer_bind     complement(2314..2334)
                     /label=BG10634
     primer_bind     complement(2314..2334)
                     /label=BG24895
     primer_bind     2324..2344
                     /label=BG12744
     primer_bind     2324..2344
                     /label=BG10647
     primer_bind     complement(2327..2350)
                     /label=BG13246
     primer_bind     2328..2348
                     /label=BG13269
     primer_bind     2329..2349
                     /label=BG10646
     primer_bind     complement(2329..2349)
                     /label=BG10631
     primer_bind     2330..2359
                     /label=BG12536
     primer_bind     2334..2354
                     /label=BG10645
     primer_bind     complement(2334..2354)
                     /label=BG10630
     primer_bind     complement(2339..2359)
                     /label=BG10629
     primer_bind     2340..2399
                     /label=BG16247
     primer_bind     complement(2340..2399)
                     /label=BG16248
     primer_bind     2344..2363
                     /label=BG13172
     primer_bind     2349..2369
                     /label=BG10642
     primer_bind     complement(2349..2369)
                     /label=BG10627
     primer_bind     complement(2351..2394)
                     /label=BG21597
     misc_feature    complement(2364..3224)
                     /label=AmpR
     primer_bind     2364..2386
                     /label=BG28781
     primer_bind     complement(2369..2389)
                     /label=BG4803b
     primer_bind     complement(2371..2390)
                     /label=BG25496
     primer_bind     2373..2394
                     /label=BG21600
     primer_bind     2377..2394
                     /label=BG14411
     primer_bind     2379..2398
                     /label=BG10162
     primer_bind     complement(2387..2406)
                     /label=BG17150
     primer_bind     2395..2415
                     /label=BG26405
     primer_bind     2402..2425
                     /label=BG25710
     primer_bind     complement(2407..2427)
                     /label=BG13729
     primer_bind     complement(2413..2431)
                     /label=BG13732
     primer_bind     complement(2417..2437)
                     /label=BG22904
     primer_bind     complement(2433..2453)
                     /label=BG21308
     primer_bind     complement(2433..2453)
                     /label=BG12878
     primer_bind     complement(2434..2452)
                     /label=BG25709
     primer_bind     complement(2434..2452)
                     /label=BG28761
     primer_bind     2449..2468
                     /label=BG15466
     primer_bind     complement(2454..2473)
                     /label=BG15116
     primer_bind     complement(2454..2473)
                     /label=BG17702
     primer_bind     complement(2461..2481)
                     /label=BG10973
     primer_bind     complement(2462..2481)
                     /label=BG13727
     primer_bind     complement(2471..2491)
                     /label=BG15435
     primer_bind     2485..2502
                     /label=BG26118
     primer_bind     complement(2485..2502)
                     /label=BG26148
     primer_bind     complement(2492..2509)
                     /label=BG27441
     primer_bind     2492..2508
                     /label=BG27438
     primer_bind     2570..2591
                     /label=BG17118
     primer_bind     complement(2600..2623)
                     /label=BG14978
     primer_bind     2622..2644
                     /label=BG11135
     primer_bind     complement(2661..2680)
                     /label=BG17120
     primer_bind     2671..2688
                     /label=BG17103
     primer_bind     complement(2685..2704)
                     /label=BG23083
     primer_bind     2736..2756
                     /label=BG15677
     primer_bind     complement(2737..2756)
                     /label=BG17105
     primer_bind     complement(2738..2760)
                     /label=BG11134
     primer_bind     2744..2763
                     /label=BG21927
     primer_bind     complement(2785..2804)
                     /label=BG15548
     primer_bind     complement(2829..2848)
                     /label=BG21926
     primer_bind     complement(2847..2866)
                     /label=BG19417
     primer_bind     complement(2873..2893)
                     /label=BG26401
     primer_bind     2890..2912
                     /label=BG28094
     primer_bind     complement(2894..2915)
                     /label=BG28095
     primer_bind     2978..2997
                     /label=BG17125
     primer_bind     2984..3011
                     /label=BG22047
     primer_bind     complement(2984..3008)
                     /label=BG22048
     primer_bind     2986..3005
                     /label=BG14412
     primer_bind     complement(3022..3041)
                     /label=BG17529
     primer_bind     complement(3045..3064)
                     /label=BG13028
     primer_bind     3065..3084
                     /label=BG28107
     primer_bind     complement(3090..3108)
                     /label=BG17149
     primer_bind     3134..3152
                     /label=BG26136
     primer_bind     complement(3134..3152)
                     /label=BG26167
     primer_bind     3138..3158
                     /label=BG10971
     primer_bind     3141..3158
                     /label=BG21407
     primer_bind     3200..3221
                     /label=BG26119
     primer_bind     complement(3200..3221)
                     /label=BG26149
     misc_feature    complement(3225..3329)
                     /label=Ampp
     primer_bind     3225..3259
                     /label=BG26026
     primer_bind     3248..3271
                     /label=BG21603
     primer_bind     3251..3276
                     /label=BG26426
     primer_bind     3254..3278
                     /label=BG23146
     primer_bind     complement(3262..3281)
                     /label=BG10972
     primer_bind     complement(3266..3294)
                     /label=BG13170
     primer_bind     3266..3292
                     /label=BG16783
     primer_bind     3273..3292
                     /label=BG10949
     primer_bind     complement(3278..3302)
                     /label=BG13280
     primer_bind     complement(3292..3309)
                     /label=BG13565
     primer_bind     3300..3327
                     /label=BG11137
     primer_bind     complement(3303..3329)
                     /label=BG28780
     primer_bind     complement(3303..3328)
                     /label=BG23240
     primer_bind     3316..3340
                     /label=BG13285
     primer_bind     3324..3349
                     /label=BG13236
     primer_bind     complement(3332..3350)
                     /label=BG15099
     primer_bind     complement(3338..3355)
                     /label=BG11501
     rep_origin      complement(3403..4141)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori"
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
      181 tgagctaact tacattaatt gcgttgcgcc gactcctgca ttaggaaatg acaaacaaca
      241 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt
      301 aaagcggata acaatttcac acaggaggcc gtttacactt tatgcttccg gctcgtataa
      361 tgtgtgggga aaccacaacg gtttccctct agaaataatt ttgtttaact ataagaagga
      421 gatatacata tgactcacca tgctgatcat gactgccagc cgcacaggga gagcgttcag
      481 ccggatgaaa tccagtgtat cctggttctg gacctgagca tcgataacgc aatcaccgca
      541 tgtagcgtta ccccgcatct gccccgtgct gcacgtcgtg ttgaactaca tctgaacgac
      601 tttggtgcag aacgtgcacc gtacgggggt gcaagcgatc gtcgtacctg gcgttgttgg
      661 atgcaggcag ttgatgcaat gctggctgac gcacgtgcac agctgggtgc agaagttgaa
      721 tttactcatt attatctggc aggtcgtgca gcactcccgg tttttgcata tctgggtctg
      781 agactgggta aacaggcaaa catcactacc gttaaccgtc gtgatgacgg ttgttgggat
      841 gttgttccgt gccagcgtcc ggcttcgagc gcagcatcgg gggtttcgcc cagcgcacgt
      901 ttttttgacg aagttcgtgg tctggacacc gatgagcgta gcagcgaaag cggtatggtt
      961 gcagtttggg taagcaccca gcgtgatgtt gatcgtgggc tgctgcgtgc atttgcaagg
     1021 gcaagaggtg accgtgacct ggcaggtatc gttagcctgc gtgcacgtcc ggctgcaggt
     1081 gatgacaccg gtgatatgcg tctcctggaa ggtgcagacg gccccgatgc tgcacgtgaa
     1141 ctggttaact gttttcgtag catcccgaac cagtatccgc gtagcagcgg cctgatggtt
     1201 tttgttagcg gcccggttac cctggcagca atggttgggc gtgcaatcaa cccgagaatc
     1261 catggcccgg tttggtggcc gtactttcgt ggtggggagt acgagccggc actggaatat
     1321 ccgtggccgc tgatcagcgg ccccccgagg atcctgatcg caaccgcaaa cgctccggag
     1381 ggtgaaaacc cgactctgga cgttgaggca gagctgaagc atctggaaga agcactggca
     1441 gaaccgcgta aacgtaaact gtgtgaggta cagcgttgcc cggcagcaac cgtaagcgat
     1501 atcaccagcg cactgcgtag ctttaaaccg catatactgc attttatcgg tcatggtact
     1561 gcactcggtg tttatctgag gagcgcagaa catgacggtg cacagtttgt tcgtggtgag
     1621 gatttccagc agatgatcgc aacctcgcta cgtcagaagg accgtgaaat gcatctggtt
     1681 gttctgaacg cttgctgcac ccacgaactg gcaaaggcac tgaccgaaca ggttagctgc
     1741 accatcggta ccgatatcga agtttatgat tcggcaagca tacattttgc tgcacgtttt
     1801 tatgatcacc tggttcatgg taccagcgtt cattatgcat ttaacgcagc agttgatgaa
     1861 tgtcgtgcac acagcaccag cggccaggaa gttttttgcc tgcacccggc agcagaacgt
     1921 gcaccggcaa gcgcaacccc gccggtacgt gcagatgaac tggttttctt tagcccggca
     1981 gaacgtcata gctaatgccg tgggacagcc cgtcttaatt aacctaggct gctgccaccg
     2041 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     2101 tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     2161 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     2221 catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat tgttagacag attatcaaaa
     2281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
     2521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtagct
     3361 agctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca
     3421 aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa aacagcaggg
     3481 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac
     3541 gcttttccgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg
     3601 aaacccgaca ggacttaaag atccccaccg tttccggcgg gtcgctccct cttgcgctct
     3661 cctgttccga ccctgccgtt taccggatac ctgttccgcc tttctccctt acgggaagtg
     3721 tggcgctttc tcatagctca cacactggta tctcggctcg gtgtaggtcg ttcgctccaa
     3781 gctgggctgt aagcaagaac tccccgttca gcccgactgc tgcgccttat ccggtaactg
     3841 ttcacttgag tccaacccgg aaaagcacgg taaaacgcca ctggcagcag ccattggtaa
     3901 ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca
     3961 aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca
     4021 cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt
     4081 ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4141 c
//
