LOCUS       Exported                4576 bp ds-DNA     circular SYN 18-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(33..79)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     46..64
                     /label=BG28135
     promoter        123..153
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(177..223)
                     /label=BG31236
     CDS             221..2221
                     /codon_start=1
                     /label=PCaspase
                     /label=caspase-like
                     /note="product: 
                     MKRALLIGAPLAHSATHALAGVANDIDRAGEILRLYGFHCDDVLFGERATRDGIVARLE
                     ALIAETQADDAVVIYFSGHGGRVVNTGIVKDLPGGDYRPGAHQFLVPEDYQPKAQTFTG
                     ILDFELRYLVARLAARTENVSVILDCCHSGGAIRAPGRPTPKALDEKSLKPHTVHLNRV
                     IRERLVQLRESLAASRAPRLTQDAHPHLVRIEAAHEFRLAYETRINNRQQGVLTAAWAD
                     TLWRYRGLAVSWQALASEIQARARAFVPMQYVHIDGPTERRLFSLAREPARHSLAIVQE
                     RDGTLWLDGGRFQGVASGARFAVLPPTSTESDIDSALAEVRAVEVMPERTRVELATAAD
                     DIPRWQGLHAWPLHARGAWTGVAVRAQTAAVCAALLRAIEASQRMHLCPDSDGPVATVI
                     ELSSDEGSRIAVLDGHGVCVSPPVATPERALALVERLQRAALVRMLGSGTDEHALPIPL
                     ALRLERADGIGGTCRHLAEGRTEPPSELLLASAGAQVHAIVENHGSAIATDTQDVYLTM
                     FCIAADGRVERVSKQQSSGIRLCGGAKHRLEYELGAGGASLPEYRAADSSTSGYGLHSL
                     IVVATDRQQELRNLETWSGEGSAAKLQPGVLDLTASKRAPARRGSASASGPLRYAVLRV
                     DLRVAAAVAGRDAPTL"
                     /translation="MMKRALLIGAPLAHSATHALAGVANDIDRAGEILRLYGFHCDDVL
                     FGERATRDGIVARLEALIAETQADDAVVIYFSGHGGRVVNTGIVKDLPGGDYRPGAHQF
                     LVPEDYQPKAQTFTGILDFELRYLVARLAARTENVSVILDCCHSGGAIRAPGRPTPKAL
                     DEKSLKPHTVHLNRVIRERLVQLRESLAASRAPRLTQDAHPHLVRIEAAHEFRLAYETR
                     INNRQQGVLTAAWADTLWRYRGLAVSWQALASEIQARARAFVPMQYVHIDGPTERRLFS
                     LAREPARHSLAIVQERDGTLWLDGGRFQGVASGARFAVLPPTSTESDIDSALAEVRAVE
                     VMPERTRVELATAADDIPRWQGLHAWPLHARGAWTGVAVRAQTAAVCAALLRAIEASQR
                     MHLCPDSDGPVATVIELSSDEGSRIAVLDGHGVCVSPPVATPERALALVERLQRAALVR
                     MLGSGTDEHALPIPLALRLERADGIGGTCRHLAEGRTEPPSELLLASAGAQVHAIVENH
                     GSAIATDTQDVYLTMFCIAADGRVERVSKQQSSGIRLCGGAKHRLEYELGAGGASLPEY
                     RAADSSTSGYGLHSLIVVATDRQQELRNLETWSGEGSAAKLQPGVLDLTASKRAPARRG
                     SASASGPLRYAVLRVDLRVAAAVAGRDAPTL"
     primer_bind     386..397
                     /label=BG28352
     primer_bind     complement(433..454)
                     /label=BG29007
     primer_bind     597..616
                     /label=BG28352
     primer_bind     complement(635..655)
                     /label=BG29009
     primer_bind     complement(651..671)
                     /label=BG30990
     primer_bind     complement(651..671)
                     /label=BG31269
     misc_feature    677..679
                     /label=R152
     primer_bind     679..695
                     /label=BG31235
     primer_bind     692..708
                     /label=BG309389
     primer_bind     692..708
                     /label=BG31268
     primer_bind     1034..1089
                     /label=BG28991
     primer_bind     complement(1038..1093)
                     /label=BG28992
     primer_bind     1054..1073
                     /label=BG28353
     primer_bind     1577..1597
                     /label=BG28354
     misc_feature    2244..2254
                     /label=MCS-2
                     /note="label: MCS-2"
     terminator      2278..2325
                     /label=T7 terminator
                     /note="label: T7 terminator"
     primer_bind     2496..2525
                     /label=BG12696
     primer_bind     2496..2517
                     /label=BG13247
     primer_bind     complement(2496..2516)
                     /label=BG17673
     primer_bind     complement(2497..2517)
                     /label=BG15746
     primer_bind     complement(2497..2517)
                     /label=BG13272
     primer_bind     complement(2497..2517)
                     /label=BG13276
     primer_bind     complement(2498..2517)
                     /label=BG12166
     primer_bind     complement(2502..2517)
                     /label=BG14660
     primer_bind     complement(2505..2525)
                     /label=BG10641
     primer_bind     complement(2510..2530)
                     /label=BG10640
     primer_bind     complement(2514..2534)
                     /label=BG26402
     primer_bind     complement(2514..2534)
                     /label=BG15429
     primer_bind     2515..2535
                     /label=BG15090
     primer_bind     2515..2535
                     /label=BG10654
     primer_bind     complement(2515..2535)
                     /label=BG10639
     primer_bind     2516..2536
                     /label=BG13273
     primer_bind     2516..2536
                     /label=BG13277
     primer_bind     2516..2536
                     /label=BG14645
     primer_bind     2516..2531
                     /label=BG14675
     primer_bind     2517..2537
                     /label=BG17674
     primer_bind     complement(2518..2553)
                     /label=BG12381
     primer_bind     2518..2541
                     /label=BG12172
     primer_bind     complement(2518..2541)
                     /label=BG11057
     primer_bind     2520..2540
                     /label=BG10653
     primer_bind     complement(2520..2540)
                     /label=BG10638
     primer_bind     complement(2524..2549)
                     /label=BG12170
     primer_bind     complement(2525..2545)
                     /label=BG10637
     primer_bind     complement(2527..2547)
                     /label=BG15427
     primer_bind     2528..2548
                     /label=BG15088
     primer_bind     complement(2530..2550)
                     /label=BG10636
     primer_bind     2534..2557
                     /label=BG26135
     primer_bind     complement(2534..2557)
                     /label=BG26166
     primer_bind     2535..2555
                     /label=BG10650
     primer_bind     complement(2535..2555)
                     /label=BG10635
     primer_bind     complement(2535..2555)
                     /label=BG15744
     primer_bind     complement(2535..2555)
                     /label=BG13268
     primer_bind     complement(2539..2559)
                     /label=BG16242
     primer_bind     2540..2560
                     /label=BG10649
     primer_bind     2540..2560
                     /label=BG24896
     primer_bind     2540..2560
                     /label=BG13567
     primer_bind     2540..2560
                     /label=BG16240
     primer_bind     complement(2540..2560)
                     /label=BG24895
     primer_bind     complement(2540..2560)
                     /label=BG13566
     primer_bind     complement(2540..2560)
                     /label=BG10634
     primer_bind     2550..2570
                     /label=BG12744
     primer_bind     2550..2570
                     /label=BG10647
     primer_bind     complement(2553..2576)
                     /label=BG13246
     primer_bind     2554..2574
                     /label=BG13269
     primer_bind     2555..2575
                     /label=BG10646
     primer_bind     complement(2555..2575)
                     /label=BG10631
     primer_bind     2556..2585
                     /label=BG12536
     primer_bind     2560..2580
                     /label=BG10645
     primer_bind     complement(2560..2580)
                     /label=BG10630
     primer_bind     complement(2565..2585)
                     /label=BG10629
     primer_bind     2566..2625
                     /label=BG16247
     primer_bind     complement(2566..2625)
                     /label=BG16248
     primer_bind     2570..2589
                     /label=BG13172
     primer_bind     2575..2595
                     /label=BG10642
     primer_bind     complement(2575..2595)
                     /label=BG10627
     primer_bind     complement(2577..2620)
                     /label=BG21597
     CDS             complement(2590..3450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2590..2612
                     /label=BG28781
     primer_bind     complement(2595..2615)
                     /label=BG4803b
     primer_bind     complement(2597..2616)
                     /label=BG25496
     primer_bind     2599..2620
                     /label=BG21600
     primer_bind     2603..2620
                     /label=BG14411
     primer_bind     2605..2624
                     /label=BG10162
     primer_bind     complement(2613..2632)
                     /label=BG17150
     primer_bind     2621..2641
                     /label=BG26405
     primer_bind     2628..2651
                     /label=BG25710
     primer_bind     complement(2633..2653)
                     /label=BG13729
     primer_bind     complement(2639..2657)
                     /label=BG13732
     primer_bind     complement(2643..2663)
                     /label=BG22904
     primer_bind     complement(2659..2679)
                     /label=BG12878
     primer_bind     complement(2659..2679)
                     /label=BG21308
     primer_bind     complement(2660..2678)
                     /label=BG25709
     primer_bind     complement(2660..2678)
                     /label=BG28761
     primer_bind     2675..2694
                     /label=BG15466
     primer_bind     complement(2680..2699)
                     /label=BG17702
     primer_bind     complement(2680..2699)
                     /label=BG15116
     primer_bind     complement(2687..2707)
                     /label=BG10973
     primer_bind     complement(2688..2707)
                     /label=BG13727
     primer_bind     complement(2697..2717)
                     /label=BG15435
     primer_bind     2711..2728
                     /label=BG26118
     primer_bind     complement(2711..2728)
                     /label=BG26148
     primer_bind     complement(2718..2735)
                     /label=BG27441
     primer_bind     2718..2734
                     /label=BG27438
     primer_bind     2796..2817
                     /label=BG17118
     primer_bind     complement(2826..2849)
                     /label=BG14978
     primer_bind     2848..2870
                     /label=BG11135
     primer_bind     complement(2887..2906)
                     /label=BG17120
     primer_bind     2897..2914
                     /label=BG17103
     primer_bind     complement(2911..2930)
                     /label=BG23083
     primer_bind     2962..2982
                     /label=BG15677
     primer_bind     complement(2963..2982)
                     /label=BG17105
     primer_bind     complement(2964..2986)
                     /label=BG11134
     primer_bind     2970..2989
                     /label=BG21927
     primer_bind     complement(3011..3030)
                     /label=BG15548
     primer_bind     complement(3055..3074)
                     /label=BG21926
     primer_bind     complement(3073..3092)
                     /label=BG19417
     primer_bind     complement(3099..3119)
                     /label=BG26401
     primer_bind     3116..3138
                     /label=BG28094
     primer_bind     complement(3120..3141)
                     /label=BG28095
     primer_bind     3204..3223
                     /label=BG17125
     primer_bind     3210..3237
                     /label=BG22047
     primer_bind     complement(3210..3234)
                     /label=BG22048
     primer_bind     3212..3231
                     /label=BG14412
     primer_bind     complement(3248..3267)
                     /label=BG17529
     primer_bind     complement(3271..3290)
                     /label=BG13028
     primer_bind     3291..3310
                     /label=BG28107
     primer_bind     complement(3316..3334)
                     /label=BG17149
     primer_bind     3360..3378
                     /label=BG26136
     primer_bind     complement(3360..3378)
                     /label=BG26167
     primer_bind     3364..3384
                     /label=BG10971
     primer_bind     3367..3384
                     /label=BG21407
     primer_bind     3426..3447
                     /label=BG26119
     primer_bind     complement(3426..3447)
                     /label=BG26149
     promoter        complement(3451..3555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3451..3485
                     /label=BG26026
     primer_bind     3474..3497
                     /label=BG21603
     primer_bind     3477..3502
                     /label=BG26426
     primer_bind     3480..3504
                     /label=BG23146
     primer_bind     complement(3488..3507)
                     /label=BG10972
     primer_bind     complement(3492..3520)
                     /label=BG13170
     primer_bind     3492..3518
                     /label=BG16783
     primer_bind     3499..3518
                     /label=BG10949
     primer_bind     complement(3504..3528)
                     /label=BG13280
     primer_bind     complement(3518..3535)
                     /label=BG13565
     primer_bind     3526..3553
                     /label=BG11137
     primer_bind     complement(3529..3555)
                     /label=BG28780
     primer_bind     complement(3529..3554)
                     /label=BG23240
     primer_bind     3542..3566
                     /label=BG13285
     primer_bind     3550..3575
                     /label=BG13236
     primer_bind     complement(3558..3576)
                     /label=BG15099
     primer_bind     complement(3564..3581)
                     /label=BG11501
     rep_origin      complement(3629..4367)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori"
ORIGIN
        1 cgactcctgc attaggaaat gacaaacaac agataaaacg aaaggcccag tctttcgact
       61 gagcctttcg ttttatttga tgcctttaat taaagcggat aacaatttca cacaggaggc
      121 cgtttacact ttatgcttcc ggctcgtata atgtgtgggg aaaccacaac ggtttccctc
      181 tagaaataat tttgtttaac tataagaagg agatatacat atgatgaaaa gggctctgct
      241 gataggtgca ccgctggcac atagcgcaac ccatgcactg gcaggtgttg caaacgatat
      301 cgaccgtgca ggtgaaatcc tgcgtctgta tggttttcat tgtgatgacg ttctctttgg
      361 tgaacgtgca actagagatg gtatcgttgc acgtctggaa gcactgatcg ctgaaaccca
      421 ggctgatgat gctgttgtta tctattttag cggccatggt ggccgtgttg ttaacaccgg
      481 gatcgttaaa gatctaccgg gtggtgacta tcgtcccggc gctcaccagt tcctggttcc
      541 ggaagactac caaccgaaag cacagacctt taccggtatc ctggacttcg agctgaggta
      601 tctagttgca cgtctggcag cacgtaccga aaacgttagc gttatcctgg attgttgtca
      661 tagcggtggt gcaataagag caccgggtag accgaccccg aaagcactgg atgaaaaaag
      721 cctgaagccg catactgttc acctgaaccg tgttatccgt gaacgtctgg ttcaactgcg
      781 tgaatcgctg gcagctagcc gtgcaccgcg tctgacccag gatgctcatc cgcatctggt
      841 tcgtatcgaa gcagcacacg aattccgtct cgcatatgaa accaggatca acaaccgtca
      901 gcagggggtt ctgaccgcag catgggcaga caccctgtgg agataccgtg ggctggcagt
      961 tagctggcag gcactggcaa gcgagatcca ggcaagggca cgtgcatttg ttcccatgca
     1021 gtatgttcac atcgatggcc cgaccgaacg tcgtctgttc agcctggctc gtgagccggc
     1081 acgtcatagc ctggcaatcg ttcaggaaag ggacggtacc ctgtggctgg acggtggcag
     1141 atttcagggt gttgcaagcg gtgcacgttt cgcagttctg ccgccgacca gcaccgaaag
     1201 cgacatcgat agcgcactgg cagaagttcg tgcagttgaa gttatgccgg aacgtacccg
     1261 tgttgaactg gctaccgcag cagatgacat cccgagatgg caaggcctcc atgcatggcc
     1321 gctgcatgca cgtggcgctt ggaccggtgt agcagttcgt gctcagaccg cagcagtttg
     1381 tgctgcactc ctgagagcta tcgaagcaag ccagcgtatg catctgtgtc cggatagcga
     1441 cgggccggtt gctaccgtta tcgaactatc gagcgatgag ggcagcagga tcgcagttct
     1501 ggatgggcac ggtgtttgtg ttagcccccc ggttgcaacc cccgaacgtg cactcgcact
     1561 ggttgaaagg ctccagagag ctgcactcgt tcgtatgctg gggagcggta ccgatgagca
     1621 tgcactaccg atcccgctcg cactcagact ggaacgtgca gacggtatcg gtggtacctg
     1681 ccgtcatcta gcagaagggc gtaccgaacc gccgagcgaa ctgctactcg caagcgctgg
     1741 ggcacaggta catgcaatcg tagagaacca tgggagcgct atcgcaaccg atacccagga
     1801 tgtttatctg accatgttct gtatcgcagc agacggtcgt gttgaaagag ttagcaagca
     1861 gcagagcagc gggatccgtc tgtgtggggg tgcaaagcat aggctcgagt atgaactagg
     1921 tgcagggggt gcaagcctgc cggaatacag ggcagcagat tcgagcacca gcggctatgg
     1981 gctgcatagc ctcatagttg ttgcaaccga ccgtcagcag gagctgcgta acctggaaac
     2041 ctggtcgggt gaaggtagcg ctgcaaaact gcagccgggt gttctggatc tgaccgctag
     2101 caaacgtgca ccggctagac gtggctcggc tagcgcaagc gggccgctga gatatgctgt
     2161 tctacgtgta gatctgcgtg tagctgcagc agtagcaggg cgtgatgcac cgaccctgta
     2221 atgccgtggg acagcccgtc ttaattaacc taggctgctg ccaccgctga gcaataacta
     2281 gcataacccc ttggggcctc taaacgggtc ttgagtggtt ttttgctgaa acctcaggca
     2341 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     2401 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc gtggccggat
     2461 cttgcggccc ctcggcttga acgaattgtt agacagatta tcaaaaagga tcttcaccta
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2701 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtagctagct cactcggtcg
     3601 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
     3661 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg
     3721 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca
     3781 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
     3841 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct
     3901 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat
     3961 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
     4021 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
     4081 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
     4141 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
     4201 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
     4261 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
     4321 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     4381 cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
     4441 gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg
     4501 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
     4561 attaattgcg ttgcgc
//
