LOCUS       Exported                6487 bp ds-DNA     circular SYN 15-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Carl Salazar
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(242..288)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        332..362
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             430..1995
                     /codon_start=1
                     /label=SAVED-CHAT
                     /note="product: 
                     THHADHDCQPHRESVQPDEIQCILVLDLSIDNAITACSVTPHLPRAARRVELHLNDFGA
                     ERAPYGGASDRRTWRCWMQAVDAMLADARAQLGAEVEFTHYYLAGRAALPVFAYLGLRL
                     GKQANITTVNRRDDGCWDVVPCQRPASSAASGVSPSARFFDEVRGLDTDERSSESGMVA
                     VWVSTQRDVDRGLLRAFARARGDRDLAGIVSLRARPAAGDDTGDMRLLEGADGPDAARE
                     LVNCFRSIPNQYPRSSGLMVFVSGPVTLAAMVGRAINPRIHGPVWWPYFRGGEYEPALE
                     YPWPLISGPPRILIATANAPEGENPTLDVEAELKHLEEALAEPRKRKLCEVQRCPAATV
                     SDITSALRSFKPHILHFIGHGTALGVYLRSAEHDGAQFVRGEDFQQMIATSLRQKDREM
                     HLVVLNACCTHELAKALTEQVSCTIGTDIEVYDSASIHFAARFYDHLVHGTSVHYAFNA
                     AVDECRAHSTSGQEVFCLHPAAERAPASATPPVRADELVFFSPAERHS"
                     /translation="MTHHADHDCQPHRESVQPDEIQCILVLDLSIDNAITACSVTPHLP
                     RAARRVELHLNDFGAERAPYGGASDRRTWRCWMQAVDAMLADARAQLGAEVEFTHYYLA
                     GRAALPVFAYLGLRLGKQANITTVNRRDDGCWDVVPCQRPASSAASGVSPSARFFDEVR
                     GLDTDERSSESGMVAVWVSTQRDVDRGLLRAFARARGDRDLAGIVSLRARPAAGDDTGD
                     MRLLEGADGPDAARELVNCFRSIPNQYPRSSGLMVFVSGPVTLAAMVGRAINPRIHGPV
                     WWPYFRGGEYEPALEYPWPLISGPPRILIATANAPEGENPTLDVEAELKHLEEALAEPR
                     KRKLCEVQRCPAATVSDITSALRSFKPHILHFIGHGTALGVYLRSAEHDGAQFVRGEDF
                     QQMIATSLRQKDREMHLVVLNACCTHELAKALTEQVSCTIGTDIEVYDSASIHFAARFY
                     DHLVHGTSVHYAFNAAVDECRAHSTSGQEVFCLHPAAERAPASATPPVRADELVFFSPA
                     ERHS"
     primer_bind     complement(1671..1692)
                     /label=BG29120
                     /note="RV SAVED-CHAT C422A"
     misc_feature    1996..2027
                     /label=RBS 20000
     CDS             2028..4028
                     /codon_start=1
                     /label=PCaspase
                     /label=caspase-like
                     /note="product: 
                     MKRALLIGAPLAHSATHALAGVANDIDRAGEILRLYGFHCDDVLFGERATRDGIVARLE
                     ALIAETQADDAVVIYFSGHGGRVVNTGIVKDLPGGDYRPGAHQFLVPEDYQPKAQTFTG
                     ILDFELRYLVARLAARTENVSVILDCCHSGGAIRAPGRPTPKALDEKSLKPHTVHLNRV
                     IRERLVQLRESLAASRAPRLTQDAHPHLVRIEAAHEFRLAYETRINNRQQGVLTAAWAD
                     TLWRYRGLAVSWQALASEIQARARAFVPMQYVHIDGPTERRLFSLAREPARHSLAIVQE
                     RDGTLWLDGGRFQGVASGARFAVLPPTSTESDIDSALAEVRAVEVMPERTRVELATAAD
                     DIPRWQGLHAWPLHARGAWTGVAVRAQTAAVCAALLRAIEASQRMHLCPDSDGPVATVI
                     ELSSDEGSRIAVLDGHGVCVSPPVATPERALALVERLQRAALVRMLGSGTDEHALPIPL
                     ALRLERADGIGGTCRHLAEGRTEPPSELLLASAGAQVHAIVENHGSAIATDTQDVYLTM
                     FCIAADGRVERVSKQQSSGIRLCGGAKHRLEYELGAGGASLPEYRAADSSTSGYGLHSL
                     IVVATDRQQELRNLETWSGEGSAAKLQPGVLDLTASKRAPARRGSASASGPLRYAVLRV
                     DLRVAAAVAGRDAPTL"
                     /translation="MMKRALLIGAPLAHSATHALAGVANDIDRAGEILRLYGFHCDDVL
                     FGERATRDGIVARLEALIAETQADDAVVIYFSGHGGRVVNTGIVKDLPGGDYRPGAHQF
                     LVPEDYQPKAQTFTGILDFELRYLVARLAARTENVSVILDCCHSGGAIRAPGRPTPKAL
                     DEKSLKPHTVHLNRVIRERLVQLRESLAASRAPRLTQDAHPHLVRIEAAHEFRLAYETR
                     INNRQQGVLTAAWADTLWRYRGLAVSWQALASEIQARARAFVPMQYVHIDGPTERRLFS
                     LAREPARHSLAIVQERDGTLWLDGGRFQGVASGARFAVLPPTSTESDIDSALAEVRAVE
                     VMPERTRVELATAADDIPRWQGLHAWPLHARGAWTGVAVRAQTAAVCAALLRAIEASQR
                     MHLCPDSDGPVATVIELSSDEGSRIAVLDGHGVCVSPPVATPERALALVERLQRAALVR
                     MLGSGTDEHALPIPLALRLERADGIGGTCRHLAEGRTEPPSELLLASAGAQVHAIVENH
                     GSAIATDTQDVYLTMFCIAADGRVERVSKQQSSGIRLCGGAKHRLEYELGAGGASLPEY
                     RAADSSTSGYGLHSLIVVATDRQQELRNLETWSGEGSAAKLQPGVLDLTASKRAPARRG
                     SASASGPLRYAVLRVDLRVAAAVAGRDAPTL"
     primer_bind     2193..2204
                     /label=BG28352
                     /note="CASPASE-like seq1"
     primer_bind     complement(2240..2261)
                     /label=BG29007
                     /note="RV caspase-like H78A"
     primer_bind     2404..2423
                     /label=BG28352
                     /note="CASPASE-like seq1"
     primer_bind     complement(2442..2462)
                     /label=BG29009
                     /note="RV caspase-like C145A"
     primer_bind     2861..2880
                     /label=BG28353
                     /note="CASPASE-like seq2"
     primer_bind     3384..3404
                     /label=BG28354
                     /note="CASPASE-like seq3"
     primer_bind     complement(4012..4053)
                     /label=BG31406
     primer_bind     4029..4074
                     /label=BG30194
     primer_bind     4029..4052
                     /label=BG31405
     CDS             4054..4497
                     /codon_start=1
                     /label=PCi
                     /translation="MHRIDVSGPLYLDCQQSGDTVQLIHQTTVLVEPAIELYGTDTSTT
                     LEIRDFEDENGRCRVNVFTIERDGTLTPWERLASGAVSPAVWSIIERGGERAALLVPVP
                     YAPLGELTADLVDQFAPQDYTPSRVRIVLPPPEDDKPPPHDDQ"
     primer_bind     complement(4480..4497)
                     /label=BG30195
     primer_bind     complement(4480..4497)
                     /label=BG31144
     primer_bind     4498..4518
                     /label=BG31407
     primer_bind     4498..4509
                     /label=BG30190
     primer_bind     complement(4499..4519)
                     /label=BG22374
                     /note="pTarget VA VB rv"
     primer_bind     complement(4501..4519)
                     /label=BG23609
                     /note="T7 terminator, reverse primer"
     primer_bind     complement(4501..4519)
                     /label=BG29138
                     /note="Seq purification 7011"
     terminator      4515..4562
                     /label=T7 terminator
                     /note="label: T7 terminator"
     CDS             complement(4710..5570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5571..5675)
                     /label=Ampp
     rep_origin      complement(5749..6487)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori"
ORIGIN
        1 ttttctactg aaccgctcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
       61 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
      121 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
      181 tgagctaact tacattaatt gcgttgcgcc gactcctgca ttaggaaatg acaaacaaca
      241 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt
      301 aaagcggata acaatttcac acaggaggcc gtttacactt tatgcttccg gctcgtataa
      361 tgtgtgggga aaccacaacg gtttccctct agaaataatt ttgtttaact ataagaagga
      421 gatatacata tgactcacca tgctgatcat gactgccagc cgcacaggga gagcgttcag
      481 ccggatgaaa tccagtgtat cctggttctg gacctgagca tcgataacgc aatcaccgca
      541 tgtagcgtta ccccgcatct gccccgtgct gcacgtcgtg ttgaactaca tctgaacgac
      601 tttggtgcag aacgtgcacc gtacgggggt gcaagcgatc gtcgtacctg gcgttgttgg
      661 atgcaggcag ttgatgcaat gctggctgac gcacgtgcac agctgggtgc agaagttgaa
      721 tttactcatt attatctggc aggtcgtgca gcactcccgg tttttgcata tctgggtctg
      781 agactgggta aacaggcaaa catcactacc gttaaccgtc gtgatgacgg ttgttgggat
      841 gttgttccgt gccagcgtcc ggcttcgagc gcagcatcgg gggtttcgcc cagcgcacgt
      901 ttttttgacg aagttcgtgg tctggacacc gatgagcgta gcagcgaaag cggtatggtt
      961 gcagtttggg taagcaccca gcgtgatgtt gatcgtgggc tgctgcgtgc atttgcaagg
     1021 gcaagaggtg accgtgacct ggcaggtatc gttagcctgc gtgcacgtcc ggctgcaggt
     1081 gatgacaccg gtgatatgcg tctcctggaa ggtgcagacg gccccgatgc tgcacgtgaa
     1141 ctggttaact gttttcgtag catcccgaac cagtatccgc gtagcagcgg cctgatggtt
     1201 tttgttagcg gcccggttac cctggcagca atggttgggc gtgcaatcaa cccgagaatc
     1261 catggcccgg tttggtggcc gtactttcgt ggtggggagt acgagccggc actggaatat
     1321 ccgtggccgc tgatcagcgg ccccccgagg atcctgatcg caaccgcaaa cgctccggag
     1381 ggtgaaaacc cgactctgga cgttgaggca gagctgaagc atctggaaga agcactggca
     1441 gaaccgcgta aacgtaaact gtgtgaggta cagcgttgcc cggcagcaac cgtaagcgat
     1501 atcaccagcg cactgcgtag ctttaaaccg catatactgc attttatcgg tcatggtact
     1561 gcactcggtg tttatctgag gagcgcagaa catgacggtg cacagtttgt tcgtggtgag
     1621 gatttccagc agatgatcgc aacctcgcta cgtcagaagg accgtgaaat gcatctggtt
     1681 gttctgaacg cttgctgcac ccacgaactg gcaaaggcac tgaccgaaca ggttagctgc
     1741 accatcggta ccgatatcga agtttatgat tcggcaagca tacattttgc tgcacgtttt
     1801 tatgatcacc tggttcatgg taccagcgtt cattatgcat ttaacgcagc agttgatgaa
     1861 tgtcgtgcac acagcaccag cggccaggaa gttttttgcc tgcacccggc agcagaacgt
     1921 gcaccggcaa gcgcaacccc gccggtacgt gcagatgaac tggttttctt tagcccggca
     1981 gaacgtcata gctaataaca tacgcgtaac aaggtaagga caaattaatg atgaaaaggg
     2041 ctctgctgat aggtgcaccg ctggcacata gcgcaaccca tgcactggca ggtgttgcaa
     2101 acgatatcga ccgtgcaggt gaaatcctgc gtctgtatgg ttttcattgt gatgacgttc
     2161 tctttggtga acgtgcaact agagatggta tcgttgcacg tctggaagca ctgatcgctg
     2221 aaacccaggc tgatgatgct gttgttatct attttagcgg ccatggtggc cgtgttgtta
     2281 acaccgggat cgttaaagat ctaccgggtg gtgactatcg tcccggcgct caccagttcc
     2341 tggttccgga agactaccaa ccgaaagcac agacctttac cggtatcctg gacttcgagc
     2401 tgaggtatct agttgcacgt ctggcagcac gtaccgaaaa cgttagcgtt atcctggatt
     2461 gttgtcatag cggtggtgca ataagagcac cgggtagacc gaccccgaaa gcactggatg
     2521 aaaaaagcct gaagccgcat actgttcacc tgaaccgtgt tatccgtgaa cgtctggttc
     2581 aactgcgtga atcgctggca gctagccgtg caccgcgtct gacccaggat gctcatccgc
     2641 atctggttcg tatcgaagca gcacacgaat tccgtctcgc atatgaaacc aggatcaaca
     2701 accgtcagca gggggttctg accgcagcat gggcagacac cctgtggaga taccgtgggc
     2761 tggcagttag ctggcaggca ctggcaagcg agatccaggc aagggcacgt gcatttgttc
     2821 ccatgcagta tgttcacatc gatggcccga ccgaacgtcg tctgttcagc ctggctcgtg
     2881 agccggcacg tcatagcctg gcaatcgttc aggaaaggga cggtaccctg tggctggacg
     2941 gtggcagatt tcagggtgtt gcaagcggtg cacgtttcgc agttctgccg ccgaccagca
     3001 ccgaaagcga catcgatagc gcactggcag aagttcgtgc agttgaagtt atgccggaac
     3061 gtacccgtgt tgaactggct accgcagcag atgacatccc gagatggcaa ggcctccatg
     3121 catggccgct gcatgcacgt ggcgcttgga ccggtgtagc agttcgtgct cagaccgcag
     3181 cagtttgtgc tgcactcctg agagctatcg aagcaagcca gcgtatgcat ctgtgtccgg
     3241 atagcgacgg gccggttgct accgttatcg aactatcgag cgatgagggc agcaggatcg
     3301 cagttctgga tgggcacggt gtttgtgtta gccccccggt tgcaaccccc gaacgtgcac
     3361 tcgcactggt tgaaaggctc cagagagctg cactcgttcg tatgctgggg agcggtaccg
     3421 atgagcatgc actaccgatc ccgctcgcac tcagactgga acgtgcagac ggtatcggtg
     3481 gtacctgccg tcatctagca gaagggcgta ccgaaccgcc gagcgaactg ctactcgcaa
     3541 gcgctggggc acaggtacat gcaatcgtag agaaccatgg gagcgctatc gcaaccgata
     3601 cccaggatgt ttatctgacc atgttctgta tcgcagcaga cggtcgtgtt gaaagagtta
     3661 gcaagcagca gagcagcggg atccgtctgt gtgggggtgc aaagcatagg ctcgagtatg
     3721 aactaggtgc agggggtgca agcctgccgg aatacagggc agcagattcg agcaccagcg
     3781 gctatgggct gcatagcctc atagttgttg caaccgaccg tcagcaggag ctgcgtaacc
     3841 tggaaacctg gtcgggtgaa ggtagcgctg caaaactgca gccgggtgtt ctggatctga
     3901 ccgctagcaa acgtgcaccg gctagacgtg gctcggctag cgcaagcggg ccgctgagat
     3961 atgctgttct acgtgtagat ctgcgtgtag ctgcagcagt agcagggcgt gatgcaccga
     4021 ccctgtaaat cacatgaata aggaggatgt cgtatgcaca ggattgacgt ttcgggtccg
     4081 ctgtacctgg attgtcaaca gagcggggac accgttcagc tgattcacca aaccaccgtt
     4141 ctggttgaac cggcaattga actatacggg accgatacca gcaccaccct ggaaattcgt
     4201 gactttgaag atgaaaacgg aagatgtcgt gttaacgttt ttaccattga acgtgacggt
     4261 accctgaccc cgtgggaacg tctagcaagc ggagcagttt cgccggcagt ttggagcatt
     4321 attgagcgtg gaggggagcg tgctgctctg ctggttccgg ttccgtacgc accgctgggt
     4381 gaactgaccg cagacctggt tgatcaattt gcaccgcagg attacacccc gtcgagggtt
     4441 agaattgttc tcccgccgcc ggaagacgat aaaccgccgc cgcacgatga ccaataacca
     4501 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     4561 tggggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt agacagatta
     4621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc
     4861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5701 gtagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca
     5761 catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca
     5821 gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa
     5881 acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta
     5941 cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg
     6001 cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg
     6061 gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg
     6121 ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg
     6181 taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat
     6241 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     6301 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     6361 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
     6421 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     6481 tttgatc
//
