LOCUS       Exported                3244 bp ds-DNA     circular SYN 18-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEcNucC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    123..152
                     /label=lacUV5 promoter
     misc_feature    221..946
                     /label=EcNucC
                     /note="Genbank EFJ98159"
     primer_bind     718..735
                     /label=BG29862
     misc_feature    969..979
                     /label=MCS-2
                     /note="label: MCS-2"
     terminator      1003..1050
                     /label=T7 terminator
                     /note="label: T7 terminator"
     primer_bind     1221..1250
                     /label=BG12696
     primer_bind     1221..1242
                     /label=BG13247
     primer_bind     complement(1221..1241)
                     /label=BG17673
     primer_bind     complement(1222..1242)
                     /label=BG13276
     primer_bind     complement(1222..1242)
                     /label=BG13272
     primer_bind     complement(1222..1242)
                     /label=BG15746
     primer_bind     complement(1223..1242)
                     /label=BG12166
     primer_bind     complement(1227..1242)
                     /label=BG14660
     primer_bind     complement(1230..1250)
                     /label=BG10641
     primer_bind     complement(1235..1255)
                     /label=BG10640
     primer_bind     complement(1239..1259)
                     /label=BG26402
     primer_bind     complement(1239..1259)
                     /label=BG15429
     primer_bind     1240..1260
                     /label=BG10654
     primer_bind     1240..1260
                     /label=BG15090
     primer_bind     complement(1240..1260)
                     /label=BG10639
     primer_bind     1241..1261
                     /label=BG13277
     primer_bind     1241..1261
                     /label=BG14645
     primer_bind     1241..1261
                     /label=BG13273
     primer_bind     1241..1256
                     /label=BG14675
     primer_bind     1242..1262
                     /label=BG17674
     primer_bind     complement(1243..1278)
                     /label=BG12381
     primer_bind     1243..1266
                     /label=BG12172
     primer_bind     complement(1243..1266)
                     /label=BG11057
     primer_bind     1245..1265
                     /label=BG10653
     primer_bind     complement(1245..1265)
                     /label=BG10638
     primer_bind     complement(1249..1274)
                     /label=BG12170
     primer_bind     complement(1250..1270)
                     /label=BG10637
     primer_bind     complement(1252..1272)
                     /label=BG15427
     primer_bind     1253..1273
                     /label=BG15088
     primer_bind     complement(1255..1275)
                     /label=BG10636
     primer_bind     1259..1282
                     /label=BG26135
     primer_bind     complement(1259..1282)
                     /label=BG26166
     primer_bind     1260..1280
                     /label=BG10650
     primer_bind     complement(1260..1280)
                     /label=BG15744
     primer_bind     complement(1260..1280)
                     /label=BG10635
     primer_bind     complement(1260..1280)
                     /label=BG13268
     primer_bind     complement(1264..1284)
                     /label=BG16242
     primer_bind     1265..1285
                     /label=BG10649
     primer_bind     1265..1285
                     /label=BG16240
     primer_bind     1265..1285
                     /label=BG13567
     primer_bind     1265..1285
                     /label=BG24896
     primer_bind     complement(1265..1285)
                     /label=BG10634
     primer_bind     complement(1265..1285)
                     /label=BG24895
     primer_bind     complement(1265..1285)
                     /label=BG13566
     primer_bind     1275..1295
                     /label=BG12744
     primer_bind     1275..1295
                     /label=BG10647
     primer_bind     complement(1278..1301)
                     /label=BG13246
     primer_bind     1279..1299
                     /label=BG13269
     primer_bind     1280..1300
                     /label=BG10646
     primer_bind     complement(1280..1300)
                     /label=BG10631
     primer_bind     1281..1310
                     /label=BG12536
     primer_bind     1285..1305
                     /label=BG10645
     primer_bind     complement(1285..1305)
                     /label=BG10630
     primer_bind     complement(1290..1310)
                     /label=BG10629
     primer_bind     1291..1350
                     /label=BG16247
     primer_bind     complement(1291..1350)
                     /label=BG16248
     primer_bind     1295..1314
                     /label=BG13172
     primer_bind     1300..1320
                     /label=BG10642
     primer_bind     complement(1300..1320)
                     /label=BG10627
     primer_bind     complement(1302..1345)
                     /label=BG21597
     misc_feature    complement(1315..2175)
                     /label=AmpR
     primer_bind     1315..1337
                     /label=BG28781
     primer_bind     complement(1320..1340)
                     /label=BG4803b
     primer_bind     complement(1322..1341)
                     /label=BG25496
     primer_bind     1324..1345
                     /label=BG21600
     primer_bind     1328..1345
                     /label=BG14411
     primer_bind     1330..1349
                     /label=BG10162
     primer_bind     complement(1338..1357)
                     /label=BG17150
     primer_bind     1346..1366
                     /label=BG26405
     primer_bind     1353..1376
                     /label=BG25710
     primer_bind     complement(1358..1378)
                     /label=BG13729
     primer_bind     complement(1364..1382)
                     /label=BG13732
     primer_bind     complement(1368..1388)
                     /label=BG22904
     primer_bind     complement(1384..1404)
                     /label=BG12878
     primer_bind     complement(1384..1404)
                     /label=BG21308
     primer_bind     complement(1385..1403)
                     /label=BG25709
     primer_bind     complement(1385..1403)
                     /label=BG28761
     primer_bind     1400..1419
                     /label=BG15466
     primer_bind     complement(1405..1424)
                     /label=BG17702
     primer_bind     complement(1405..1424)
                     /label=BG15116
     primer_bind     complement(1412..1432)
                     /label=BG10973
     primer_bind     complement(1413..1432)
                     /label=BG13727
     primer_bind     complement(1422..1442)
                     /label=BG15435
     primer_bind     1436..1453
                     /label=BG26118
     primer_bind     complement(1436..1453)
                     /label=BG26148
     primer_bind     complement(1443..1460)
                     /label=BG27441
     primer_bind     1443..1459
                     /label=BG27438
     primer_bind     1521..1542
                     /label=BG17118
     primer_bind     complement(1551..1574)
                     /label=BG14978
     primer_bind     1573..1595
                     /label=BG11135
     primer_bind     complement(1612..1631)
                     /label=BG17120
     primer_bind     1622..1639
                     /label=BG17103
     primer_bind     complement(1636..1655)
                     /label=BG23083
     primer_bind     1687..1707
                     /label=BG15677
     primer_bind     complement(1688..1707)
                     /label=BG17105
     primer_bind     complement(1689..1711)
                     /label=BG11134
     primer_bind     1695..1714
                     /label=BG21927
     primer_bind     complement(1736..1755)
                     /label=BG15548
     primer_bind     complement(1780..1799)
                     /label=BG21926
     primer_bind     complement(1798..1817)
                     /label=BG19417
     primer_bind     complement(1824..1844)
                     /label=BG26401
     primer_bind     1841..1863
                     /label=BG28094
     primer_bind     complement(1845..1866)
                     /label=BG28095
     primer_bind     1929..1948
                     /label=BG17125
     primer_bind     1935..1962
                     /label=BG22047
     primer_bind     complement(1935..1959)
                     /label=BG22048
     primer_bind     1937..1956
                     /label=BG14412
     primer_bind     complement(1973..1992)
                     /label=BG17529
     primer_bind     complement(1996..2015)
                     /label=BG13028
     primer_bind     2016..2035
                     /label=BG28107
     primer_bind     complement(2041..2059)
                     /label=BG17149
     primer_bind     2085..2103
                     /label=BG26136
     primer_bind     complement(2085..2103)
                     /label=BG26167
     primer_bind     2089..2109
                     /label=BG10971
     primer_bind     2092..2109
                     /label=BG21407
     primer_bind     2151..2172
                     /label=BG26119
     primer_bind     complement(2151..2172)
                     /label=BG26149
     promoter        complement(2176..2280)
                     /label=Ampp
     primer_bind     2176..2210
                     /label=BG26026
     primer_bind     2199..2222
                     /label=BG21603
     primer_bind     2202..2227
                     /label=BG26426
     primer_bind     2205..2229
                     /label=BG23146
     primer_bind     complement(2213..2232)
                     /label=BG10972
     primer_bind     complement(2217..2245)
                     /label=BG13170
     primer_bind     2217..2243
                     /label=BG16783
     primer_bind     2224..2243
                     /label=BG10949
     primer_bind     complement(2229..2253)
                     /label=BG13280
     primer_bind     complement(2243..2260)
                     /label=BG13565
     primer_bind     2251..2278
                     /label=BG11137
     primer_bind     complement(2254..2280)
                     /label=BG28780
     primer_bind     complement(2254..2279)
                     /label=BG23240
     primer_bind     2267..2291
                     /label=BG13285
     primer_bind     2275..2300
                     /label=BG13236
     primer_bind     complement(2283..2301)
                     /label=BG15099
     primer_bind     complement(2289..2306)
                     /label=BG11501
     rep_origin      complement(2354..3035)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="direction: LEFT label: CloDF13 ori"
ORIGIN
        1 cgactcctgc attaggaaat gacaaacaac agataaaacg aaaggcccag tctttcgact
       61 gagcctttcg ttttatttga tgcctttaat taaagcggat aacaatttca cacaggaggc
      121 cgtttacact ttatgcttcc ggctcgtata atgtgtgggg aaaccacaac ggtttccctc
      181 tagaaataat tttgtttaac tataagaagg agatatacat atgtcagatt ggtcgctttc
      241 tcaactattt gcatcgttac atgaagacat tcagcttcga ctgggaactg cacgtaaagc
      301 tttccagcat cccggggcta aaggggatgc cagtgaagga gtatggattg aaatgcttga
      361 tacatatctt cccaaacgct atcaggcagc taatgctttt gtagttgata gtttgggtaa
      421 ttttagtgat cagattgatg tggttgtttt tgacaggcag tactcaccat tcattttcaa
      481 atttaatgaa caaataattg ttccagcaga aagtgtctat gcggtttttg aagcaaagca
      541 atctgcgtca gcagatcttg ttgcttatgc acagcgaaaa gttgcaagtg ttcgcagact
      601 tcatcgtacc agccttccaa ttccgcatgc tggcgggaca tatccagcaa aaccactcat
      661 tccaattttg ggagggttac ttacttttga aagtgactgg agtcctgcat tggggatgtc
      721 gtttgacaaa gccctcaatg gggatttatc tgatggacga cttgatatgg gctgtgttgc
      781 atcgcatggg catttttact tcaataacat tgatagcaag ttcaattttg aacatggaaa
      841 taaaccagct accgcttttc ttttcaggct tattgcgcaa cttcaattta gtggcactgt
      901 gccaatgatt gacattgatg cctatggaaa atggttggct aattgatgcc gtgggacagc
      961 ccgtcttaat taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg
     1021 gcctctaaac gggtcttgag tggttttttg ctgaaacctc aggcatttga gaagcacacg
     1081 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     1141 taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg
     1201 cttgaacgaa ttgttagaca gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1321 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1381 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1441 tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1501 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1561 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1621 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1681 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1741 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1801 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1861 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1921 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1981 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2041 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2101 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2161 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2221 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2281 cacatttccc cgaaaagtgc cacctgtagc tagctcactc ggtcgctacg ctccgggcgt
     2341 gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca
     2401 ggggactaac atgtgaggca aaacagcagg gccgcgccgg tggcgttttt ccataggctc
     2461 cgccctcctg ccagagttca cataaacaga cgcttttccg gtgcatctgt gggagccgtg
     2521 aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc
     2581 gtttccggcg ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata
     2641 cctgttccgc ctttctccct tacgggaagt gtggcgcttt ctcatagctc acacactggt
     2701 atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc
     2761 agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg gaaaagcacg
     2821 gtaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     2881 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     2941 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     3001 cttcaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt
     3061 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3121 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     3181 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     3241 gcgc
//
