LOCUS       Exported                2575 bp ds-DNA     circular SYN 18-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1..23
                     /label=BG28781
     primer_bind     complement(6..26)
                     /label=BG4803b
     primer_bind     complement(8..27)
                     /label=BG25496
     primer_bind     10..31
                     /label=BG21600
     primer_bind     14..31
                     /label=BG14411
     primer_bind     16..35
                     /label=BG10162
     primer_bind     complement(24..43)
                     /label=BG17150
     primer_bind     32..52
                     /label=BG26405
     primer_bind     39..62
                     /label=BG25710
     primer_bind     complement(44..64)
                     /label=BG13729
     primer_bind     complement(50..68)
                     /label=BG13732
     primer_bind     complement(54..74)
                     /label=BG22904
     primer_bind     complement(70..90)
                     /label=BG12878
     primer_bind     complement(70..90)
                     /label=BG21308
     primer_bind     complement(71..89)
                     /label=BG28761
     primer_bind     complement(71..89)
                     /label=BG25709
     primer_bind     86..105
                     /label=BG15466
     primer_bind     complement(91..110)
                     /label=BG17702
     primer_bind     complement(91..110)
                     /label=BG15116
     primer_bind     complement(98..118)
                     /label=BG10973
     primer_bind     complement(99..118)
                     /label=BG13727
     primer_bind     complement(108..128)
                     /label=BG15435
     primer_bind     122..139
                     /label=BG26118
     primer_bind     complement(122..139)
                     /label=BG26148
     primer_bind     complement(129..146)
                     /label=BG27441
     primer_bind     129..145
                     /label=BG27438
     primer_bind     207..228
                     /label=BG17118
     primer_bind     complement(237..260)
                     /label=BG14978
     primer_bind     259..281
                     /label=BG11135
     primer_bind     complement(298..317)
                     /label=BG17120
     primer_bind     308..325
                     /label=BG17103
     primer_bind     complement(322..341)
                     /label=BG23083
     primer_bind     373..393
                     /label=BG15677
     primer_bind     complement(374..393)
                     /label=BG17105
     primer_bind     complement(375..397)
                     /label=BG11134
     primer_bind     381..400
                     /label=BG21927
     primer_bind     complement(422..441)
                     /label=BG15548
     primer_bind     complement(466..485)
                     /label=BG21926
     primer_bind     complement(484..503)
                     /label=BG19417
     primer_bind     complement(510..530)
                     /label=BG26401
     primer_bind     527..549
                     /label=BG28094
     primer_bind     complement(531..552)
                     /label=BG28095
     primer_bind     615..634
                     /label=BG17125
     primer_bind     621..648
                     /label=BG22047
     primer_bind     complement(621..645)
                     /label=BG22048
     primer_bind     623..642
                     /label=BG14412
     primer_bind     complement(659..678)
                     /label=BG17529
     primer_bind     complement(682..701)
                     /label=BG13028
     primer_bind     702..721
                     /label=BG28107
     primer_bind     complement(727..745)
                     /label=BG17149
     primer_bind     771..789
                     /label=BG26136
     primer_bind     complement(771..789)
                     /label=BG26167
     primer_bind     775..795
                     /label=BG10971
     primer_bind     778..795
                     /label=BG21407
     primer_bind     837..858
                     /label=BG26119
     primer_bind     complement(837..858)
                     /label=BG26149
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     862..896
                     /label=BG26026
     primer_bind     885..908
                     /label=BG21603
     primer_bind     888..913
                     /label=BG26426
     primer_bind     891..915
                     /label=BG23146
     primer_bind     complement(899..918)
                     /label=BG10972
     primer_bind     complement(903..931)
                     /label=BG13170
     primer_bind     903..929
                     /label=BG16783
     primer_bind     910..929
                     /label=BG10949
     primer_bind     complement(915..939)
                     /label=BG13280
     primer_bind     complement(929..946)
                     /label=BG13565
     primer_bind     937..964
                     /label=BG11137
     primer_bind     complement(940..966)
                     /label=BG28780
     primer_bind     complement(940..965)
                     /label=BG23240
     primer_bind     953..977
                     /label=BG13285
     primer_bind     961..986
                     /label=BG13236
     primer_bind     complement(969..987)
                     /label=BG15099
     primer_bind     complement(975..992)
                     /label=BG11501
     rep_origin      complement(1040..1778)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     terminator      complement(2020..2066)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2110..2140
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      2264..2311
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2482..2511
                     /label=BG12696
     primer_bind     2482..2503
                     /label=BG13247
     primer_bind     complement(2482..2502)
                     /label=BG17673
     primer_bind     complement(2483..2503)
                     /label=BG15746
     primer_bind     complement(2483..2503)
                     /label=BG13272
     primer_bind     complement(2483..2503)
                     /label=BG13276
     primer_bind     complement(2484..2503)
                     /label=BG12166
     primer_bind     complement(2488..2503)
                     /label=BG14660
     primer_bind     complement(2491..2511)
                     /label=BG10641
     primer_bind     complement(2496..2516)
                     /label=BG10640
     primer_bind     complement(2500..2520)
                     /label=BG26402
     primer_bind     complement(2500..2520)
                     /label=BG15429
     primer_bind     2501..2521
                     /label=BG15090
     primer_bind     2501..2521
                     /label=BG10654
     primer_bind     complement(2501..2521)
                     /label=BG10639
     primer_bind     2502..2522
                     /label=BG13273
     primer_bind     2502..2522
                     /label=BG14645
     primer_bind     2502..2522
                     /label=BG13277
     primer_bind     2502..2517
                     /label=BG14675
     primer_bind     2503..2523
                     /label=BG17674
     primer_bind     complement(2504..2539)
                     /label=BG12381
     primer_bind     2504..2527
                     /label=BG12172
     primer_bind     complement(2504..2527)
                     /label=BG11057
     primer_bind     2506..2526
                     /label=BG10653
     primer_bind     complement(2506..2526)
                     /label=BG10638
     primer_bind     complement(2510..2535)
                     /label=BG12170
     primer_bind     complement(2511..2531)
                     /label=BG10637
     primer_bind     complement(2513..2533)
                     /label=BG15427
     primer_bind     2514..2534
                     /label=BG15088
     primer_bind     complement(2516..2536)
                     /label=BG10636
     primer_bind     2520..2543
                     /label=BG26135
     primer_bind     complement(2520..2543)
                     /label=BG26166
     primer_bind     2521..2541
                     /label=BG10650
     primer_bind     complement(2521..2541)
                     /label=BG15744
     primer_bind     complement(2521..2541)
                     /label=BG10635
     primer_bind     complement(2521..2541)
                     /label=BG13268
     primer_bind     complement(2525..2545)
                     /label=BG16242
     primer_bind     2526..2546
                     /label=BG24896
     primer_bind     2526..2546
                     /label=BG16240
     primer_bind     2526..2546
                     /label=BG13567
     primer_bind     2526..2546
                     /label=BG10649
     primer_bind     complement(2526..2546)
                     /label=BG13566
     primer_bind     complement(2526..2546)
                     /label=BG24895
     primer_bind     complement(2526..2546)
                     /label=BG10634
     primer_bind     2536..2556
                     /label=BG12744
     primer_bind     2536..2556
                     /label=BG10647
     primer_bind     complement(2539..2562)
                     /label=BG13246
     primer_bind     2540..2560
                     /label=BG13269
     primer_bind     2541..2561
                     /label=BG10646
     primer_bind     complement(2541..2561)
                     /label=BG10631
     primer_bind     2542..2571
                     /label=BG12536
     primer_bind     2546..2566
                     /label=BG10645
     primer_bind     complement(2546..2566)
                     /label=BG10630
     primer_bind     complement(2551..2571)
                     /label=BG10629
     primer_bind     join(2552..2575,1..36)
                     /label=BG16247
     primer_bind     complement(join(2552..2575,1..36))
                     /label=BG16248
     primer_bind     2556..2575
                     /label=BG13172
     primer_bind     join(2561..2575,1..6)
                     /label=BG10642
     primer_bind     complement(join(2561..2575,1..6))
                     /label=BG10627
     primer_bind     complement(join(2563..2575,1..31))
                     /label=BG21597
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtagctagc tcactcggtc gctacgctcc
     1021 gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga
     1081 taagcagggg actaacatgt gaggcaaaac agcagggccg cgccggtggc gtttttccat
     1141 aggctccgcc ctcctgccag agttcacata aacagacgct tttccggtgc atctgtggga
     1201 gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc
     1261 cccaccgttt ccggcgggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac
     1321 cggatacctg ttccgccttt ctcccttacg ggaagtgtgg cgctttctca tagctcacac
     1381 actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc
     1441 ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa
     1501 agcacggtaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt
     1561 ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac
     1621 agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga
     1681 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     1741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgctctaga
     1801 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     1861 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1921 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1981 gttgcgccga ctcctgcatt aggaaatgac aaacaacaga taaaacgaaa ggcccagtct
     2041 ttcgactgag cctttcgttt tatttgatgc ctttaattaa agcggataac aatttcacac
     2101 aggaggccgt ttacacttta tgcttccggc tcgtataatg tgtggggaaa ccacaacggt
     2161 ttccctctag aaataatttt gtttaactat aagaaggaga tatacattgc cgtgggacag
     2221 cccgtcttaa ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     2281 ggcctctaaa cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac
     2341 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     2401 ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg cggcccctcg
     2461 gcttgaacga attgttagac agattatcaa aaaggatctt cacctagatc cttttaaatt
     2521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacag
//
