LOCUS       Exported                6256 bp DNA     circular SYN 26-FEB-2024
DEFINITION  In-Fusion enabled vector for expression in E.coli, mmalin or insect 
            cells with C-His tag.
ACCESSION   .
VERSION     .
KEYWORDS    pOPINE-3C-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   nick berrow
  TITLE     Direct Submission
  JOURNAL   Exported Feb 26, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     In-Fusion enabled vector for expression in mammalian cells with 
            C-His tag. 
            http://www.informaxinc.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     45..1011
                     /label=lef2/ORF 603
                     /label=lef2/ORF\603
                     /note="lef2/ORF 603"
                     /note="/vntifkey=86"
     enhancer        1079..1443
                     /label=CMV Enhancer
                     /label=CMV\Enhancer
                     /note="CMV Enhancer"
                     /note="/vntifkey=9"
     promoter        1449..1726
                     /label=Chicken ﬂ-Actin Promoter
                     /label=Chicken\ﬂ-Actin\Promoter
                     /note="Chicken ﬂ-Actin Promoter"
                     /note="/vntifkey=29"
     promoter        2150..2166
                     /label=T7 Promoter
                     /label=T7\Promoter
                     /note="T7 Promoter"
                     /note="/vntifkey=30"
     protein_bind    2171..2191
                     /label=lac Operator
                     /label=lac\Operator
                     /note="lac Operator"
                     /note="/vntifkey=31"
     promoter        2205..2318
                     /label=p10 Promoter+5'UTR
                     /label=p10\Promoter+5'UTR
                     /note="p10 Promoter+5'UTR"
                     /note="/vntifkey=29"
     CDS             2712..2731
                     /codon_start=1
                     /label=3C cleavage site
                     /label=3C\cleavage\site
                     /note="/vntifkey=21"
                     /translation="LEVLFQ"
     CDS             2736..3465
                     /codon_start=1
                     /label=mCherry
                     /note="/vntifkey=4"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYKKHHHHHH"
     polyA_signal    3519..3755
                     /label=Rabbit ﬂ-Globin Poly A Site
                     /label=Rabbit\ﬂ-Globin\Poly\A\Site
                     /note="Rabbit ﬂ-Globin Poly A Site"
                     /note="/vntifkey=25"
     terminator      3759..3806
                     /label=T7 Terminator
                     /label=T7\Terminator
                     /note="T7 Terminator"
                     /note="/vntifkey=43"
     misc_recomb     3819..4524
                     /label=ORF 1629
                     /label=ORF\1629
                     /note="ORF 1629"
                     /note="/vntifkey=86"
     rep_origin      4735
                     /label=pUC origin of replication
                     /label=pUC\origin\of\replication
                     /note="pUC origin of replication"
                     /note="/vntifkey=33"
     CDS             complement(5337..6194)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggcaca
     2341 ccatcaccac catcacagca gcggtaccgt cgacccgact ggaaagcggg cagtgagcgc
     2401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     2521 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2581 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2641 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcca
     2701 agctgtttaa actggaagtt ctgtttcagg gcccggtgag caagggcgag gaggataaca
     2761 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     2821 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2881 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     2941 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     3001 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     3061 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     3121 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg
     3181 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     3241 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     3301 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     3361 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     3421 gcatggacga gctgtacaag aaacatcacc atcaccatca ctaagtgatt aacctcaggt
     3481 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     3541 ctgagatcga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     3601 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3661 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     3721 atttggttta gagtttggca acatatgccc atatgtaact agcataaccc cttggggcct
     3781 ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct ccttgaagtt
     3841 tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac tggtccggcg
     3901 tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc tgtgcgttgt
     3961 tgatttacag acaattgttg tacgtatttt aataattcat taaatttata atctttaggg
     4021 tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt
     4081 aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc
     4141 gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct
     4201 tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg
     4261 acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat
     4321 tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct
     4381 ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac
     4441 gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa
     4501 tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata cattatacga
     4561 agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4621 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4681 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4741 aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4801 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4861 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4921 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4981 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5041 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5101 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5161 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5221 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgttaccaa
     5341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6241 ctcatgtccg cgcgtt
//
