LOCUS       Exported               13031 bp ds-DNA     circular SYN 19-DEC-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Youjun
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     637..656
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             705..725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             726..4826
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4884..4904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7005..7025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             7035..7061
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /note="color: #993366"
                     /translation="PAAKRVKLD"
     CDS             7071..7127
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 7124"
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7128..7130
                     /label=Kozak sequence
                     /note="color: #a6acb3"
     CDS             7131..7433
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53DD
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /note="color: #993366"
                     /translation="EPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERF
                     EMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     misc_feature    7131..7217
                     /label=NLS
                     /note="color: #a6acb3"
     misc_feature    7149..7433
                     /label=hP53DD part based on addgene 25989
                     /note="color: #ffff00"
     misc_feature    7218..7331
                     /label=Tet.
                     /note="color: #0000ff"
     misc_feature    7332..7433
                     /label=3' terminal
                     /note="color: #a6acb3"
     misc_feature    7452..8038
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 7452 .. 8026 / #a6acb3
                        2: 8027 .. 8029 / #a6acb3 / ATG
                        3: 8030 .. 8038 / #a6acb3"
     misc_feature    8042..10300
                     /label=MLH1dn
                     /note="color: #a6acb3"
     CDS             10306..10323
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     primer_bind     complement(10346..10363)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    10352..10576
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(10647..10663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(10647..10663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(10660..10682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    10671..10687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(10695..10725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 10695 .. 10701 / #ffffff / -10
                        2: 10702 .. 10719 / #ffffff
                        3: 10720 .. 10725 / #ffffff / -35"
     protein_bind    10740..10761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(10878..10895)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(11049..11637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(11129..11148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(11808..12668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11808 .. 12599 / #ccffcc
                        2: 12600 .. 12668 / #ccffcc / signal sequence
                     Cleavage site after base 12599"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12431..12450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(12669..12773)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(12852..12871)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
ORIGIN
        1 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      601 agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact atagggagag
      661 ccgccaccat gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga
      721 aagtcgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg
      781 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
      841 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
      901 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
      961 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     1021 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     1081 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     1141 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     1201 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     1261 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     1321 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     1381 gaaagctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     1441 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     1501 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     1561 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     1621 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     1681 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     1741 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     1801 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     1861 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaagagagag gacctgctgc
     1921 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     1981 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     2041 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     2101 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     2161 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     2221 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     2281 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     2341 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     2401 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     2461 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     2521 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     2581 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     2641 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     2701 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     2761 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     2821 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     2881 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     2941 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     3001 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     3061 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     3121 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     3181 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg
     3241 ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     3301 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     3361 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     3421 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     3481 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     3541 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     3601 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     3661 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa
     3721 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     3781 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     3841 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     3901 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     3961 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     4021 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     4081 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     4141 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     4201 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     4261 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     4321 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     4381 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     4441 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     4501 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     4561 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     4621 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     4681 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     4741 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     4801 tcgacctgtc tcagctggga ggtgactccg gcggaagctc tggtggcagc aagcggaccg
     4861 ccgacggctc tgaattcgag agccctaaga agaaaagaaa ggtgagcgga ggctctagcg
     4921 gcggaagcac cctgaacatt gaagacgagt atagactgca tgaaacaagc aaggaacccg
     4981 acgtgtccct gggctccacc tggctgtccg actttcccca ggcctgggcc gagacaggag
     5041 gaatgggcct ggccgtgcgg caggcacccc tgatcatccc tctgaaggcc acctctacac
     5101 ccgtgagcat caagcagtac cctatgtctc aggaggccag actgggcatc aagcctcaca
     5161 tccagaggct gctggaccag ggcatcctgg tgccatgcca gagcccctgg aacacaccac
     5221 tgctgcccgt gaagaagcca ggcaccaatg actatagacc cgtgcaggat ctgagagagg
     5281 tgaacaagag ggtggaggat atccacccca ccgtgcccaa cccttacaat ctgctgtccg
     5341 gcctgccccc ttctcaccag tggtatacag tgctggacct gaaggatgcc ttcttttgtc
     5401 tgagactgca ccctaccagc cagccactgt tcgcctttga gtggagggac cctgagatgg
     5461 gcatctctgg ccagctgacc tggacacgcc tgcctcaggg cttcaagaat agcccaacac
     5521 tgtttaacga ggccctgcac cgcgacctgg cagatttccg gatccagcac ccagatctga
     5581 tcctgctgca gtacgtggac gatctgctgc tggccgccac cagcgagctg gattgccagc
     5641 agggaacacg cgccctgctg cagaccctgg gaaacctggg atatagggca tccgccaaga
     5701 aggcccagat ctgtcagaag caggtgaagt acctgggcta tctgctgaag gagggccaga
     5761 gatggctgac agaggccagg aaggagacag tgatgggcca gccaacaccc aagaccccaa
     5821 gacagctgag ggagttcctg ggcaaagcag gattttgcag gctgttcatc ccaggattcg
     5881 cagagatggc agcacctctg tacccactga ccaagccggg caccctgttt aattggggcc
     5941 ctgaccagca gaaggcctat caggagatca agcaggccct gctgacagca ccagccctgg
     6001 gcctgccaga cctgaccaag cctttcgagc tgtttgtgga tgagaagcag ggctacgcca
     6061 agggcgtgct gacccagaag ctgggaccat ggagacggcc cgtggcctat ctgtccaaga
     6121 agctggaccc agtggcagca ggatggccac catgcctgag gatggtggca gcaatcgccg
     6181 tgctgacaaa ggatgccggc aagctgacca tgggacagcc actggtcatc ctggcaccac
     6241 acgcagtgga ggccctggtg aagcagcctc cagatcgctg gctgtctaac gcccggatga
     6301 cacactacca ggccctgctg ctggacaccg atcgcgtgca gtttggccct gtggtggccc
     6361 tgaatccagc caccctgctg cctctgccag aggagggcct gcagcacaac tgtctggaca
     6421 tcctggcaga ggcacacgga acaaggccag acctgaccga tcagcccctg cctgacgccg
     6481 atcacacatg gtataccgat ggaagctccc tgctgcagga gggccagagg aaggcaggag
     6541 cagcagtgac cacagagaca gaagtgatct gggccaaggc cctgccagca ggcacatccg
     6601 cccagcgggc cgagctgatc gccctgaccc aggccctgaa gatggccgag ggcaagaagc
     6661 tgaacgtgta cacagactcc agatatgcct tcgccaccgc acacatccac ggagagatct
     6721 acaggcgccg gggctggctg acctctgagg gcaaggagat caagaacaag gatgagatcc
     6781 tggccctgct gaaggccctg tttctgccca agcggctgag catcatccac tgtcctggac
     6841 accagaaggg acactccgcc gaggcaaggg gcaatcggat ggccgaccag gccgccagaa
     6901 aggctgctat tactgaaact cccgacactt ccactctgct gattgaaaac tcctcccctt
     6961 ctggcggctc aaaaagaacc gccgacggca gcgaattcga gtctcccaag aagaagagga
     7021 aagtcggctc tggccctgcc gctaagagag tgaagctgga cggatccggc gcaacaaact
     7081 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gagcctcacc
     7141 acgagctgcc cccagggagc actaagcgag cactgcccaa caacaccagc tcctctcccc
     7201 agccaaagaa gaaaccactg gatggagaat atttcaccct tcagatccgt gggcgtgagc
     7261 gcttcgagat gttccgagag ctgaatgagg ccttggaact caaggatgcc caggctggga
     7321 aggagccagg ggggagcagg gctcactcca gccacctgaa gtccaaaaag ggtcagtcta
     7381 cctcccgcca taaaaaactc atgttcaaga cagaagggcc tgactcagac tgaccggtgt
     7441 ttaggcgcgc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     7501 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     7561 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     7621 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     7681 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     7741 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     7801 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     7861 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7921 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7981 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     8041 gagcttcgtt gctggagtca tccggagact ggacgagaca gtggtgaaca gaattgccgc
     8101 cggcgaggtg atccagagac ctgccaatgc aataaaggag atgatcgaga actgtctgga
     8161 cgccaagtcc acaagcattc aggtgatcgt gaaggagggc ggactgaagc tgatccagat
     8221 ccaagacaac ggcacaggca tcagaaagga agatctggac atcgtgtgtg aacggttcac
     8281 cacatctaag ctgcagtctt ttgaggatct ggcctctatc agtacctacg gcttcagagg
     8341 cgaggccctg gccagcatca gccacgtggc ccatgtgacc atcaccacca aaaccgccga
     8401 cggcaaatgc gcttatcgcg ctagctacag cgacggcaag ctgaaagccc cgccaaagcc
     8461 ttgcgccggc aaccagggta cacagataac agtggaggat ctgttctaca acatcgccac
     8521 ccggagaaag gccctgaaaa atcccagcga ggagtacggc aagatcctgg aagtcgtcgg
     8581 cagatactcc gtgcacaacg ccggaatcag ctttagcgta aagaagcagg gagaaaccgt
     8641 ggccgatgtg cgcaccctgc caaatgccag caccgtggat aacatcagaa gcattttcgg
     8701 aaatgccgtg tccagagaac tgatcgagat cggctgcgaa gataagaccc tggcttttaa
     8761 gatgaacggc tacatcagca acgccaatta ctctgtgaag aagtgcatct ttcttctgtt
     8821 catcaaccac agactggtgg aaagcaccag cctgcggaaa gccatcgaga cagtgtacgc
     8881 cgcctacctg cctaagaaca cccacccctt cctgtacctg agcctcgaga tcagccctca
     8941 gaacgtggac gtcaatgtgc atcctacaaa gcacgaggtg cacttcctgc acgaggaaag
     9001 catcctggaa agagtgcagc agcacattga gagcaagctg ctgggctcta acagcagcag
     9061 aatgtacttc acacagaccc tgctgcctgg cctggccggc ccctcaggcg aaatggttaa
     9121 gtccacaacc tctctgacct catccagcac cagcggttct tccgataagg tgtacgccca
     9181 ccagatggtg cggaccgact ctcgggagca gaagctggac gcctttctgc aacctctgag
     9241 caaacctctg agctctcagc ctcaggccat cgtgaccgag gacaagacag atatctcctc
     9301 cggccgtgcc agacagcagg acgaagaaat gctcgagctg ccagctcctg ccgaggtggc
     9361 cgccaagaac cagagcctgg agggagatac cacaaagggc accagcgaaa tgagcgagaa
     9421 gcggggccct acctccagca accccagaaa acggcaccgg gaggacagcg acgtggaaat
     9481 ggtggaggac gacagcagaa aggaaatgac agccgcttgt acccctagaa gaagaatcat
     9541 caacctgacc tccgtgctga gcctgcagga ggagatcaac gagcagggcc acgaggtgct
     9601 gagagagatg ctgcacaatc acagcttcgt gggctgcgtg aaccctcaat gggccctggc
     9661 tcagcatcaa acaaagctgt acctgctgaa caccaccaag ctgagcgaag agctgttcta
     9721 ccagatcctc atctacgact tcgccaactt cggcgtgcta cgcctgagcg agcccgcccc
     9781 tctgtttgac ctggccatgc tggctctgga tagcccagaa agcggctgga cagaagagga
     9841 cggacctaaa gaggggctgg ctgaatacat cgtggagttc ctgaagaaaa aggccgagat
     9901 gctggccgac tacttttctc tggaaatcga cgaggaaggc aacctgatcg gcctgcctct
     9961 gctgatcgat aactacgtgc ctcccctgga aggcctgccc atcttcatcc tgagactggc
    10021 tacagaggtg aactgggacg aggaaaagga atgcttcgag tctctgagca aggagtgcgc
    10081 catgttctat agcatcagaa aacagtacat ctctgaagag agcactctgt ctggccagca
    10141 gagtgaagtg cccggaagca tccccaacag ctggaagtgg accgtggaac acatcgtgta
    10201 caaggccctg cggagccaca ttctccctcc taagcacttc accgaggacg gcaacatcct
    10261 gcagctggcc aacctgcccg acctttataa ggttttctaa ccggtcatca tcaccatcac
    10321 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    10381 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    10441 taataaaatg agaaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    10501 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    10561 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc
    10621 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa
    10741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10861 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    10981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11641 gatcctttga tcttttctac ggggtctgac actcagtgga acgaaaactc acgttaaggg
    11701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    11941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12781 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg
    12841 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    12901 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    12961 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
    13021 gtacgggcca g
//
