LOCUS       308a_AAV-hSyn1-D        6895 bp DNA     circular SYN 16-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 16, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=L-ITR
     misc_binding    150..155
                     /label=MluI
     promoter        156..624
                     /label=pSyn1(S)
     misc_feature    605..625
                     /label=oligo 188f
     misc_binding    625..630
                     /label=MluI
     misc_feature    657..690
                     /label=loxP
     misc_feature    701..734
                     /label=lox2272
     misc_feature    complement(745..3173)
                     /label=VGLUT2.miniSOG
     misc_binding    917..922
                     /label=MluI
     misc_feature    complement(2313..2351)
                     /label=linker
     misc_feature    complement(2352..2669)
                     /label=miniSOG
     misc_feature    complement(2670..2705)
                     /label=linker
     misc_feature    3198..3231
                     /label=loxP(rev)
     misc_feature    3242..3275
                     /label=lox2272(rev)
     misc_feature    3289..3897
                     /label=WPRE
     misc_feature    3324..3344
                     /label=oligo 189r
     polyA_signal    3921..4135
                     /label=bGHpA
     misc_feature    4158..4298
                     /label=R-ITR
     misc_feature    4304..4971
                     /label=pUC ori
     misc_feature    5122..5979
                     /label=AmpR
     misc_feature    6498..6804
                     /label=f1 ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctgca gagggccctg cgtatgagtg
      181 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      241 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      301 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      361 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      421 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      481 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      541 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      601 gtcgtgtcgt gcctgagagc gcagacgcgt ggacggtacc cttcaggtcg acgtcgataa
      661 cttcgtatag catacattat acgaagttat ccgaatcgca ataacttcgt ataggatact
      721 ttatacgaag ttatttggcg cgccatatta ttattatttt ttgaacagca agacttgctt
      781 ggttgatgtg tttacatgtt tacaccacat ttcttaaaat gattttgtgc aatcacaatt
      841 aatttaatca caaacactac aaaggaatcc agcaactagc ttcgttatga ataatcatct
      901 cggtccttat aggagtacgc gtcttgcgca ctttcttgca caaattcttc ctttttctcc
      961 cagccgttag gccagcctcc gttctcctgt gaggtggcgc cgtaggattt ggtggtaccg
     1021 taatttatgt aattctgagt gatgtccccc gtttcttcat ccagttcatc ttcatgaatg
     1081 aagccacact tttcttcgct tgtttcctca gggtctgccc aaggttgctt ctctcctgag
     1141 gcaaatagtg cataaaatat gactccacca tagtggacca gtgcagcgat gaggaagaca
     1201 tactgccatt cttcacggga cttgttcttc gtcattgcac caacaatgat cgggcagacc
     1261 attcccgaca gcgtgccaac accatttgaa atgcccatta agatactggc atatctcggg
     1321 gcaatatcca agtggttcac attgaaacca gagatagcaa atccactgaa tcccactgca
     1381 agcaccaaga aggagatggc cacccctcta gtatgagagt agccaacaac cagaagcagt
     1441 gtggcttcca tgccaaaacc cccgcagttc atgatctttc gcactgtagt tgttgaaaga
     1501 atttgcttgc tccttagaaa gtctgcaatt tgccccccga taggcacaat gattgtcatg
     1561 accaggtgtg ggaccgcaga caacatgcca accttgctga tttcaaatcc aaaaacctcc
     1621 tcgaaataag ctggttgact gatgagcagt aaataaaaag tccaactcct gcagaagttt
     1681 gcaacaatta tcgcatagac gggcatggat gtgaaaaact tcctccatgg ggtcttgaat
     1741 ttctccattg ctcctaacag atttgcgctc tccccgatgc tctcttctat gtacctacgt
     1801 tcttcgtctg ttatggttgg atgctttgcg gggctctcgt aagacaccag aagccagaac
     1861 atataccaga ccataccaaa gcttccatat acgtaaaata ctgaagacca tccagtgtac
     1921 tgcaccagga taccagctag gggcattgca atgactgctc cagcatagga accacagaag
     1981 gaggtggtag ccaacctact cctctccaaa ggaggggccc acttgctcca tatcccgtga
     2041 caggctgggt aggtgacgcc ctccacaagt ccttgcaata ttctaacaaa gatgacgcat
     2101 ccataatgca ctctggctgc agatgggatc agcatattga gggtagaggt aagcagtatg
     2161 gcagccccaa agacccggtt agcagccagt cgcgatgcga tgtatccgcc cggaatctgc
     2221 gtgatgatat agccccagaa gaacgacccg tgaatcatcc ccacagtctc ggggtcccag
     2281 ttaaacttgg ctttctcctt gataactttg ccgccggtcc caccgctccc gccagtgccc
     2341 ccggtacctc ctccatccag ctgcactccg atgaaatact ggagctcacc cttctggtcc
     2401 ctcatgggct ggaggtgcag gaggttccag aatttcttcc cgctttttgt gtagtttatc
     2461 agctgcacgg taatctcgcg ctgatctcta atggcatcgc gaatcttctg cactgtggcc
     2521 tggtctgtct ctggtccctg cagaaagcgg ccattgcggc ccaggatctc ctctctggaa
     2581 tactcggtca gctccaggaa gccatcggat gcgaagatga tgggattgtc tggcagccgt
     2641 ggatcagtta tcacgaaact tttctccatt gaaccaccag tgcccccaga tcccccgctc
     2701 gtgctacccc ggtggatggt gctgttgttg accatgtcca caatggccac acccaggtta
     2761 cagcggatac caaaggagat gcagaagccg aggccgctca tgatggctat gatgtagcgg
     2821 cgcggcaggc cgaagcacgt acagtcgcat agcggagcct tcttctcagg cacctccagg
     2881 ggcttgccgt cctctgtcag ctcgatggtc tctcggttat cctgcttctt ctccagcacc
     2941 ctgtagatct gtccgaggga ttttccagca aaattcttta tcccctcttt ccccggggcc
     3001 aaaatccttt gttttaccga ctccattctt gtaaagactc gtgtccagcc ttaccagatt
     3061 taaattgtga tggctatgaa aggcgagttc tgcgcatagt cttaataggt taaggtagaa
     3121 cgttttcttc tcagcagaga tgagcttttc agcaaggctg tgagttggag aggggcgcgc
     3181 caacctggcc ggccttgata acttcgtata atgtatgcta tacgaagtta tccgaatcgc
     3241 aataacttcg tataaagtat cctatacgaa gttatcgaag atcatcaagc ttatcgataa
     3301 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3361 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3421 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3481 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3541 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3661 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3721 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3781 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3841 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     3901 gctgctcgag agatcgatct gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4081 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggacacgt
     4141 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4201 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4261 ggcctcagtg agcgagcgag cgcgcagctg cctgcaggac atgtgagcaa aaggccagca
     4321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4801 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5221 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5701 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6001 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6061 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6121 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     6181 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6241 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6301 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6361 ccataaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6421 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagcc
     6481 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6541 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6601 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6661 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6721 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6781 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacgt
     6841 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgcc
//
