LOCUS       Exported                8708 bp ds-DNA     circular SYN 29-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 2, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             412..465
                     /codon_start=1
                     /label=BPSV40 NLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             466..1149
                     /codon_start=1
                     /label=Anc-APOBEC-1
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEIKWGT
                     SHKIWRHSSKNTTKHVEVNFIEKFTSERHFCPSTSCSITWFLSWSPCGECSKAITEFLS
                     QHPNVTLVIYVARLYHHMDQQNRQGLRDLVNSGVTIQIMTAPEYDYCWRNFVNYPPGKE
                     AHWPRYPPLWMKLYALELHAGILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     misc_feature    1150..1245
                     /label=linker
     CDS             1246..4404
                     /codon_start=1
                     /label=SlugCas9-NNG(D10A)
                     /translation="NQKFILGLAIGITSVGYGLIDYETKNIIDAGVRLFPEANVENNEG
                     RRSKRGSRRLKRRRIHRLERVKKLLEDYNLLDQSQIPQSTNPYAIRVKGLSEALSKDEL
                     VIALLHIAKRRGIHKIDVIDSNDDVGNELSTKEQLNKNSKLLKDKFVCQIQLERMNEGQ
                     VRGEKNRFKTADIIKEIIQLLNVQKNFHQLDENFINKYIELVEMRREYFEGPGKGSPYG
                     WEGDPKAWYETLMGHCTYFPDELRSVKYAYSADLFNALNDLNNLVIQRDGLSKLEYHEK
                     YHIIENVFKQKKKPTLKQIANEINVNPEDIKGYRITKSGKPQFTEFKLYHDLKSVLFDQ
                     SILENEDVLDQIAEILTIYQDKDSIKSKLTELDILLNEEDKENIAQLTGYTGTHRLSLK
                     CIRLVLEEQWYSSRNQMEIFTHLNIKPKKINLTAANKIPKAMIDEFILSPVVKRTFGQA
                     INLINKIIEKYGVPEDIIIELARENNSKDKQKFINEMQKKNENTRKRINEIIGKYGNQN
                     AKRLVEKIRLHDEQEGKCLYSLESIPLEDLLNNPNHYEVDHIIPRSVSFDNSYHNKVLV
                     KQSENSKKSNLTPYQYFNSGKSKLSYNQFKQHILNLSKSQDRISKKKKEYLLEERDINK
                     FEVQKEFINRNLVDTRYATRELTNYLKAYFSANNMNVKVKTINGSFTDYLRKVWKFKKE
                     RNHGYKHHAEDALIIANADFLFKENKKLKAVNSVLEKPEIESKQLDIQVDSEDNYSEMF
                     IIPKQVQDIKDFRNFKYSHRVDKKPNRRLINDTLYSTRKKDNSTYIVQTIKDIYAKDNT
                     TLKKQFDKSPEKFLMYQHDPRTFEKLEVIMKQYANEKNPLAKYHEETGEYLTKYSKKNN
                     GPIVKSLKYIGNKLGRHLDVTHQFKSSTKKLVKRSIKPYRFDVYLTDKGYKFITISYLD
                     VLKKDNYYYIPEQKYDKLKLGKAIDKNAKFIASFYKNDLIKLDGEIYKIIGVSSDTRNM
                     IELDLPDIRYKEYCELNNIKGKPRIIKTIGKKVNSIEKLTTDVLGNVFTNTQYTKPQLL
                     FKRGN"
     misc_feature    3586..3588
                     /label=Q782R
     misc_feature    3904..3905
                     /label=S888R
     misc_feature    3958..3960
                     /label=L906R
     misc_feature    4192..4194
                     /label=N984S
     misc_feature    4277..4278
                     /label=E1012K
     misc_feature    4288..4290
                     /label=K1016I
     CDS             4435..4683
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4714..4962
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5005..5025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5035..5091
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5092..5688
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             5701..5721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5730..5747
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5776..6000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6071..6087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6095..6111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6119..6149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6164..6185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6466..7054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7225..8085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8086..8190)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8460..8708
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgaaaccgga
      481 ccagtggcag tggacccaac cctgaggaga cggattgagc cccatgaatt tgaagtgttc
      541 tttgacccaa gggagctgag gaaggagaca tgcctgctgt acgagatcaa gtggggcaca
      601 agccacaaga tctggcgcca cagctccaag aacaccacaa agcacgtgga agtgaatttc
      661 atcgagaagt ttacctccga gcggcacttc tgcccctcta ccagctgttc catcacatgg
      721 tttctgtctt ggagcccttg cggcgagtgt tccaaggcca tcaccgagtt cctgtctcag
      781 caccctaacg tgaccctggt catctacgtg gcccggctgt atcaccacat ggaccagcag
      841 aacaggcagg gcctgcgcga tctggtgaat tctggcgtga ccatccagat catgacagcc
      901 ccagagtacg actattgctg gcggaacttc gtgaattatc cacctggcaa ggaggcacac
      961 tggccaagat acccacccct gtggatgaag ctgtatgcac tggagctgca cgcaggaatc
     1021 ctgggcctgc ctccatgtct gaatatcctg cggagaaagc agccccagct gacatttttc
     1081 accattgctc tgcagtcttg tcactatcag cggctgcctc ctcatattct gtgggctaca
     1141 ggcctgaagt ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc
     1201 gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcaaacca aaaattcata
     1261 ctgggactgg ccatcggaat caccagcgtg ggctacggcc tgatcgacta cgagacaaag
     1321 aatatcatcg atgccggcgt tagactgttc cccgaggcca acgtggaaaa caacgaggga
     1381 agaaggtcca aacgtggaag cagaagactg aagcgacgcc gcattcacag acttgaacgg
     1441 gtgaagaagc tgctcgagga ttataatctg ctggatcagt cccagattcc tcagtctaca
     1501 aacccctacg ccatccgcgt gaagggcctg tctgaagccc tgagcaagga cgaactcgtg
     1561 attgccctgc tccatatcgc caagagaaga ggcatccaca agatcgacgt gatcgacagc
     1621 aacgacgacg tggggaacga gctcagcacc aaggaacagc tgaataagaa cagcaagctg
     1681 ctgaaagaca aatttgtgtg ccagatccag ctggaaagaa tgaatgaggg ccaggtgcgg
     1741 ggagagaaaa accggttcaa gaccgctgat atcatcaagg aaatcatcca gctgctgaat
     1801 gtgcagaaga acttccacca gctggacgag aacttcatca acaagtacat cgaactggtt
     1861 gagatgagac gggaatactt cgagggcccc ggcaagggca gtccatatgg ctgggaaggc
     1921 gaccctaagg cttggtacga gacactgatg ggccactgca cctacttccc agatgagctg
     1981 agaagcgtga aatacgccta cagtgccgac ctgttcaacg ctctgaacga cctgaacaac
     2041 ctggtcatcc aaagagatgg actgtctaag ctcgagtatc atgagaagta tcacatcatc
     2101 gagaacgtgt tcaagcagaa gaagaaacct acactgaagc agatcgccaa tgagatcaat
     2161 gtcaaccctg aagatatcaa gggctacaga atcacaaagt ctggcaagcc ccagtttacc
     2221 gagtttaagc tctaccacga cctgaaaagc gtgctgtttg accagagcat cctggagaac
     2281 gaagacgtgc tggaccagat cgctgagatc ctgaccatct accaggacaa ggatagcatc
     2341 aaatctaagc tgacggaact ggacatcctg ctgaacgagg aagataagga aaacatcgcc
     2401 cagctgactg gctacaccgg gacccaccgg ctcagcctga aatgcatccg gctggtcctg
     2461 gaagagcagt ggtattctag ccggaaccag atggaaatct tcacccacct gaacattaag
     2521 cctaagaaga tcaacctgac agccgccaac aagatcccga aggctatgat cgacgagttc
     2581 atcctgagcc ctgtggtgaa gaggaccttc ggccaggcca ttaaccttat taacaagatc
     2641 atagaaaagt acggcgtgcc tgaagatatc atcatcgagc tggccagaga aaataatagc
     2701 aaggacaagc agaagttcat caatgagatg cagaaaaaga acgagaacac cagaaagaga
     2761 attaacgaaa tcatcggcaa gtatggcaac cagaacgcca agagactggt cgagaagatt
     2821 agactgcacg acgagcagga gggcaagtgc ctgtactcac tggaaagcat ccctctggag
     2881 gacctgctga acaaccccaa ccactacgag gtggaccaca tcattccaag atctgtgtcc
     2941 ttcgacaact cttaccacaa caaagtgctc gtgaagcaga gcgagaactc caaaaaatcc
     3001 aacctgaccc cttaccagta ctttaacagc ggcaagtcca agctctctta caaccagttt
     3061 aaacaacaca tcctgaacct gagcaagtcc caggatagaa tcagcaaaaa aaagaaagag
     3121 tatctgctgg aagaacggga catcaacaag ttcgaggtgc aaaaagagtt catcaataga
     3181 aacctggtgg atacccggta cgccacaaga gagctgacaa actacctgaa ggcctacttc
     3241 agcgccaaca atatgaacgt gaaggtgaaa acgatcaacg gcagcttcac cgattacctg
     3301 cggaaagtgt ggaagtttaa gaaggaacgg aaccacggct acaagcacca cgccgaggac
     3361 gccctgatta tcgctaatgc cgatttcctg ttcaaagaga acaagaagct gaaagccgtg
     3421 aactctgtgc tggaaaaacc tgagatcgag agcaagcagc tggatatcca ggtggatagc
     3481 gaggataact acagcgaaat gttcatcatc cctaagcagg tccaggacat caaggacttc
     3541 agaaacttca agtacagcca cagagtggac aagaagccta acagacggct gatcaacgat
     3601 acactgtaca gcacccggaa gaaggacaac tccacctaca tcgtgcagac catcaaagat
     3661 atctatgcca aagataatac caccctgaag aagcagtttg acaagtcacc cgagaagttc
     3721 ctcatgtacc aacacgatcc gcggaccttc gagaagttgg aagtgatcat gaagcagtac
     3781 gctaatgaga agaatcctct ggccaagtac cacgaggaaa caggcgagta cctgaccaaa
     3841 tacagcaaaa aaaacaacgg ccctatcgtg aaaagcctga agtacattgg aaacaagctg
     3901 ggcagacacc tagatgtgac ccaccagttc aagagcagca ccaagaagtt ggtgaagcgg
     3961 agcatcaagc cttatagatt cgacgtctac ctgaccgaca agggatataa gttcatcacc
     4021 atcagctacc tggacgtgct gaagaaagac aattactact acatacccga acagaagtac
     4081 gacaagctca aactgggcaa ggccatcgac aaaaacgcca agtttatcgc tagcttctac
     4141 aagaatgatc tgatcaagct ggacggcgag atctacaaga tcatcggcgt gagtagcgac
     4201 accagaaaca tgatcgaact ggatctgcct gacatcagat acaaagaata ctgcgagctg
     4261 aacaatatca agggcaaacc tagaatcata aagaccatcg gcaaaaaggt gaatagcatc
     4321 gaaaaactga caaccgacgt gctgggcaac gtgttcacca acacccagta cacaaaacct
     4381 cagctgctgt tcaagcgagg aaatagcggc gggagcggcg ggagcggggg gagcactaat
     4441 ctgagcgaca tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg
     4501 atgctgcctg aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg
     4561 cacaccgcct acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc
     4621 gagtataagc cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg
     4681 ctgagcggag gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag
     4741 gagacaggca agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa
     4801 gaagtgatcg gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt
     4861 accgacgaaa atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg
     4921 gtcatccagg attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga
     4981 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtcggaag cggagctact
     5041 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tatgaccgag
     5101 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5161 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5221 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5281 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5341 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5401 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5461 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5521 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5581 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5641 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctc tggtggttct
     5701 cccaagaaga aaaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc
     5761 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5821 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5881 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5941 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6001 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg
     6061 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6121 acatacgagc cggaagcata aagtgtaaag cctaggatgc ctaatgagtg agctaactca
     6181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6241 attaatgaat cggccaacgc gcgggaagag gcggtttgcg tattgggccg cttcctcgct
     6301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6661 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6901 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7081 gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8221 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     8281 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8341 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8401 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     8461 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     8521 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     8581 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     8641 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8701 agtgtatc
//
