LOCUS       pMK467_(dTAG-BT-        9455 bp DNA     circular SYN 09-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Molecular Function Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Feb 2, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9455
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     protein_bind    33..54
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(69..74,75..92,93..99)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 69 .. 74 / #ffffff / -35
                        2: 75 .. 92 / #ffffff
                        3: 93 .. 99 / #ffffff / -10"
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     131..147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        168..186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    243..442
                     /label=Tol2L200
                     /note="color: #ff6600"
     enhancer        join(464..819,820..843)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="This feature has 2 segments:
                        1: 464 .. 819 / #ffffff
                        2: 820 .. 843 / #ffffff"
     primer_bind     complement(583..601)
                     /label=(3073) CAG REV primer 1
                     /note="color: black; sequence: TCAATgggCgggggTCgTT; added: 
                     2022-10-06"
     promoter        845..1122
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(1075..1095)
                     /label=(3170) BromoTag InFusion REV
                     /note="color: black; sequence: 
                     TCTTCTCCTTCATggtgGgGGCGCCTGCACCAGCGCCGCCACCT; added: 
                     2022-08-07"
     intron          1124..2141
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     primer_bind     1146..1173
                     /label=(3170) BromoTag InFusion REV
                     /note="color: black; sequence: 
                     TCTTCTCCTTCATggtgGgGGCGCCTGCACCAGCGCCGCCACCT; added: 
                     2022-08-07"
     primer_bind     2194..2232
                     /label=(3207) Reporter Infusion 5'
                     /note="color: black; sequence: 
                     TCGAGCTCGGTACCTCGgtcgacggccaccATGGGAGTG; added: 2022-09-30"
     primer_bind     2211..2251
                     /label=(3167) dTAG InFusion FOR
                     /note="color: black; sequence: 
                     cgaattctgcagtcgacggccaccATGGGAGTGCAGGTGGAAACCATCTCCC; 
                     added: 2022-08-07"
     regulatory      2218..2227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2224..2226
                     /codon_start=1
                     /label=1st Met
                     /note="color: #a6acb3"
                     /translation="M"
     primer_bind     2226..2249
                     /label=(2997) dTAG FOR
                     /note="color: black; sequence: 
                     ggtctcgattctacgGGAGTGCAGGTGGAAACCATCTC; added: 2022-02-19"
     CDS             2227..2547
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=dTAG (FKBP12F36V)
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /note="color: #008080"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     2227..2249
                     /label=(3007) SacI-dTAG
                     /note="color: black; sequence: 
                     aattggagctcCCCGggatccggtgcaggcgccGGAGTGCAGGTGGAAACCATCTC; 
                     added: 2022-02-19"
     primer_bind     2227..2248
                     /label=(3004) BglII-dTAG
                     /note="color: black; sequence: 
                     GACCTagatctGGAGTGCAGGTGGAAACCATCT; added: 2022-02-19"
     primer_bind     complement(2525..2547)
                     /label=(2998) dTAG REV
                     /note="color: black; sequence: 
                     tccgatggatcctcaTTCCAGTTTTAGAAGCTCCACAT; added: 2022-02-10"
     primer_bind     complement(2525..2547)
                     /label=(3008) dTAG-MfeI
                     /note="color: black; sequence: 
                     agctaCAATTGttaTTCCAGTTTTAGAAGCTCCACAT; added: 2022-02-19"
     primer_bind     complement(2532..2577)
                     /label=(3005) dTAG-BamHI
                     /note="color: black; sequence: 
                     agtggatccGGCGCCTGCACCAGCGCCgccgccTTCCAGTTTTAGAAGC; added: 
                     2022-02-19"
     primer_bind     complement(2532..2554)
                     /label=(3006) dTAG-NheI
                     /note="color: black; sequence: 
                     ATAGCgctagcgccgccTTCCAGTTTTAGAAGC; added: 2022-02-19"
     primer_bind     complement(2547..2571)
                     /label=(3170) BromoTag InFusion REV
                     /note="color: black; sequence: 
                     TCTTCTCCTTCATggtgGgGGCGCCTGCACCAGCGCCGCCACCT; added: 
                     2022-08-07"
     CDS             2548..2571
                     /codon_start=1
                     /label=Linker
                     /note="color: #ff99cc"
                     /translation="GGGAGAGA"
     primer_bind     complement(2553..2598)
                     /label=(3168) dTAG InFusion REV
                     /note="color: black; sequence: 
                     GTCGGGCACGTCCTTCACagaggatccGGCGCCTGCACCAGCGCCG; added: 
                     2022-08-07"
     primer_bind     2578..2600
                     /label=(2999) BglII-BromoTag
                     /note="color: black; sequence: 
                     GACCTagatctGTGAAGGACGTGCCCGACTC; added: 2022-02-18"
     CDS             2581..2967
                     /codon_start=1
                     /label=BromoTag
                     /note="color: #ff9900"
                     /translation="VKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPF
                     YKPVDVEALGAHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPP
                     DHEVVAMARKLQDVFEMRFAKMPDE"
     primer_bind     2581..2603
                     /label=(3169) BromoTag InFusion FOR
                     /note="color: black; sequence: GTGAAGGACGTGCCCGACTCTCA; 
                     added: 2022-08-07"
     modified_base   2746..2748
                     /label=L387
                     /mod_base="OTHER"
                     /note="color: #a6acb3"
     primer_bind     complement(2965..3003)
                     /label=(3000) BromoTag-BamHI
                     /note="color: black; sequence: 
                     agtggatccGGCGCCTGCACCAGCGCCGCCACCTCCGCCTCCGCCCTC; added: 
                     2022-02-18"
     CDS             2968..3003
                     /codon_start=1
                     /label=Linker
                     /note="color: #ff99cc"
                     /translation="GGGGGGGAGAGA"
     primer_bind     complement(2979..3022)
                     /label=(3170) BromoTag InFusion REV
                     /note="color: black; sequence: 
                     TCTTCTCCTTCATggtgGgGGCGCCTGCACCAGCGCCGCCACCT; added: 
                     2022-08-07"
     primer_bind     complement(2985..3003)
                     /label=(3168) dTAG InFusion REV
                     /note="color: black; sequence: 
                     GTCGGGCACGTCCTTCACagaggatccGGCGCCTGCACCAGCGCCG; added: 
                     2022-08-07"
     primer_bind     3004..3037
                     /label=(1568) KpnI-mAID
                     /note="color: black; sequence: 
                     atcatGGTACCcaccATGAAGGAGAAGAGTGCTTGTCCTAAAG"
     misc_feature    3013..3216
                     /label=mAID
                     /note="color: #0080ff; direction: RIGHT"
     primer_bind     complement(3015..3039)
                     /label=(1354) mAID seq 2
                     /note="color: black; sequence: ATCTTTAGGACAAGCACTCTTCTCC; 
                     added: 2022-10-06"
     primer_bind     3100..3119
                     /label=(811) mAID seq 1
                     /note="color: black; sequence: AAGAACGTGATGGTTTCCTG; added:
                     2022-10-06"
     primer_bind     complement(3128..3147)
                     /label=(1449) mAID seq 3
                     /note="color: black; sequence: CGCCTCCGGGCCACCGCTTG; added:
                     2022-10-06"
     primer_bind     complement(3143..3163)
                     /label=(3170) BromoTag InFusion REV
                     /note="color: black; sequence: 
                     TCTTCTCCTTCATggtgGgGGCGCCTGCACCAGCGCCGCCACCT; added: 
                     2022-08-07"
     CDS             join(3223..3225,3226..3228,3229..3939)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3223 .. 3225 / #05fd14
                        2: 3226 .. 3228 / #05fd14 / 1a
                        3: 3229 .. 3939 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3359..3378
                     /label=(533) GFP seq 2
                     /note="color: black; sequence: AGTTCATCTGCACCACCGGC"
     primer_bind     complement(3583..3602)
                     /label=(999)_Clover_R1
                     /note="color: black; sequence: ttcagctcgatgcggttcac; added:
                     2022-10-06"
     primer_bind     3770..3789
                     /label=(345) GFP seq 1
                     /note="color: black; sequence: ACCAGCAGAACACCCCCATC; added:
                     2022-10-06"
     primer_bind     complement(3782..3801)
                     /label=(534) GFP seq 3
                     /note="color: black; sequence: GGGGCCGTCGCCGATGGGGG; added:
                     2022-10-06"
     primer_bind     3836..3855
                     /label=(812) GFP seq 4
                     /note="color: black; sequence: AGTCCGCCCTGAGCAAAGAC; added:
                     2022-10-06"
     primer_bind     complement(3914..3939)
                     /label=(2347) EGFP_R
                     /note="color: black; sequence: CTTGTACAGCTCGTCCATGCCGAGAG; 
                     added: 2022-10-06"
     primer_bind     3919..3939
                     /label=(2289) EGFP_seq1
                     /note="color: black; sequence: GGCATGGACGAGCTGTACAAG; 
                     added: 2022-10-06"
     misc_feature    3940..3951
                     /label=Linker
                     /note="color: #ff6666; direction: RIGHT"
     primer_bind     3959..3975
                     /label=(3167) dTAG InFusion FOR
                     /note="color: black; sequence: 
                     cgaattctgcagtcgacggccaccATGGGAGTGCAGGTGGAAACCATCTCCC; 
                     added: 2022-08-07"
     primer_bind     complement(3975..4029)
                     /label=(1569) BamHI-NLS
                     /note="color: black; sequence: 
                     tcgatGGATCCctaCCTGACCTTTCTTTTCTTCTTGGGG"
     CDS             3976..3996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(3977..4019)
                     /label=(3208) Repoetr Infusion 3'
                     /note="color: black; sequence: 
                     gcccgcggtaccgtcgactaCCTGACCTTTCTTTTCTTCTTGG; added: 
                     2022-09-30"
     polyA_signal    4152..4273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4189..4208)
                     /label=(315) SV40 poly A rev
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC; added:
                     2022-10-06"
     primer_bind     4226..4250
                     /label=(1254)_SV40polyA_check_F1
                     /note="color: black; sequence: ttcactgcattctagttgtggtttg"
     misc_feature    4304..4337
                     /label=LoxP-1
                     /note="note=""direct strand 
                     translation""translation=""ITSYNVCYTKLX"""
                     /note="color: #84a4c0; direction: RIGHT"
     protein_bind    complement(4304..4337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     promoter        4398..4897
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(4426..4445)
                     /label=(1075) PGK promoter seq 1
                     /note="color: black; sequence: ctaaagcgcatgctccagac"
     CDS             4943..5977
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MDRSGKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVG
                     GRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQ
                     DLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE
                     AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
                     VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRP
                     RAKE"
     primer_bind     5401..5420
                     /label=(46) Check hphNT A
                     /note="color: black; sequence: CATATGCGCGATTGCTGATC"
     polyA_signal    join(6061..6100,6101..6516)
                     /label=mouse PGK terminator
                     /note="This feature has 2 segments:
                        1: 6061 .. 6100 / #ffffff
                        2: 6101 .. 6516 / #ffffff"
     primer_bind     6472..6496
                     /label=(1078) mouse PGK term F1
                     /note="color: black; sequence: tcattctcagtattgttttgccaag"
     primer_bind     6504..6523
                     /label=(1076) PGK terminator seq 1
                     /note="color: black; sequence: tccatcagaagctggtcgag"
     protein_bind    complement(6540..6573)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(6574..6618)
                     /label=(1614) Reverse primer
                     /note="color: black; sequence: 
                     aagtaaaagcaagaaagatcTTAGTGAACCTCTTCGAGGGACCTA"
     misc_feature    6604..6778
                     /label=Tol2R175
                     /note="color: #ff6600"
     promoter        complement(6809..6827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6837..6853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      6995..7450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7476..7580
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(7581..7649,7650..8441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7581 .. 7649 / #ccffcc / signal sequence
                        2: 7650 .. 8441 / #ccffcc
                     Cleavage site after base 7649"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8612..9200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
       61 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
      181 taaagggaac aaaagctgga gctcggatct gctgggcttg ctgaaggtag ggggtcaaga
      241 accagaggtg taaagtactt gagtaatttt acttgattac tgtacttaag tattattttt
      301 ggggattttt actttacttg agtacaatta aaaatcaata cttttacttt tacttaatta
      361 cattttttta gaaaaaaaag tactttttac tccttacaat tttatttaca gtcaaaaagt
      421 acttattttt tggagatcac ttgggccctc tagatgcatg ctcgacattg attattgact
      481 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      601 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      661 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      781 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      841 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      901 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      961 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
     1021 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     1081 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct
     1141 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     1201 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     1261 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     1321 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     1381 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     1441 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1501 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1561 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1621 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1681 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     1741 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1801 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1861 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1921 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1981 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     2041 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2101 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2161 ttattgtgct gtctcatcat tttggcaaag aattcgagct cggtacctcg gtcgacggcc
     2221 accatgggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc
     2281 ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa agttgattcc
     2341 tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc
     2401 tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca
     2461 gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc
     2521 ttcgatgtgg agcttctaaa actggaaggc ggcggcgctg gtgcaggcgc cggatcctct
     2581 gtgaaggacg tgcccgactc tcagcagcac ccagcaccag agaagagcag caaggtctcg
     2641 gagcagctca agtgctgcag cggcatcctc aaggagatgt ttgccaagaa gcacgccgcc
     2701 tacgcctggc ccttctacaa gcctgtggac gtggaggcac tgggcgccca cgactactgt
     2761 gacatcatca agcaccccat ggacatgagc acaatcaagt ctaaactgga ggcccgtgag
     2821 taccgtgatg ctcaggagtt tggtgctgac gtccgattga tgttctccaa ctgctataag
     2881 tacaaccctc ctgaccatga ggtggtggcc atggcccgca agctccagga tgtgttcgaa
     2941 atgcgctttg ccaagatgcc ggacgagggc ggaggcggag gtggcggcgc tggtgcaggc
     3001 gcccccacca tgaaggagaa gagtgcttgt cctaaagatc cagccaaacc tccggccaag
     3061 gcacaagttg tgggatggcc accggtgaga tcataccgga agaacgtgat ggtttcctgc
     3121 caaaaatcaa gcggtggccc ggaggcggcg gcgttcgtga aggtatcaat ggacggagca
     3181 ccgtacttga ggaaaatcga tttgaggatg tataaagcta gcatggtgag caagggcgag
     3241 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3301 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3361 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3421 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3481 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3541 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3601 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3661 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     3721 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3781 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3841 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3901 gccgccggga tcactctcgg catggacgag ctgtacaagg gtgcaggcgc ccaagcttcg
     3961 aattctgcag tcgaccccaa gaagaaaaga aaggtcaggt agtcgacggt accgcgggcc
     4021 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     4081 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4141 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4261 tcaatgtatc ttaacgcgtc gatcatattc aataaccctt aatataactt cgtataatgt
     4321 atgctatacg aagttattag gtctgaagag gagtttacgt ccagccaagc ttaggatctc
     4381 gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     4441 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4501 cacatccacc ggtaggcgcc aaccgactcc gttctttggt ggccccttcg cgccaccttc
     4561 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     4621 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     4681 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     4741 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     4801 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     4861 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc tagatctcga
     4921 gcagctgaag cttaccgcta gcatggatag atccggaaag cctgaactca ccgcgacgtc
     4981 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5041 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5101 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5161 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5221 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5281 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5341 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5401 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5461 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5521 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5581 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5641 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5701 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5761 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5821 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     5881 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     5941 ccgacgcccc agcactcgtc cgagggcaaa ggaataggct agccgcccgc cccacgaccc
     6001 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga
     6061 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
     6121 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
     6181 ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt
     6241 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
     6301 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
     6361 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
     6421 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
     6481 gtattgtttt gccaagttct aattccatca gaagctggtc gagatccgga acccttaata
     6541 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgaagagg ttcactaaga
     6601 tctttcttgc ttttactttt acttccttaa tactcaagta caattttaat ggagtacttt
     6661 tttactttta ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt
     6721 tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgtc
     6781 aagaaccata tgccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6841 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6901 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6961 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7021 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7081 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7141 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7201 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7261 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7321 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7381 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7441 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7561 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7621 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7681 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7741 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7801 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7861 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7921 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7981 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8041 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8101 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8161 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8221 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8281 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8341 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8401 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8461 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8521 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8581 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8641 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8701 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8761 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8821 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8881 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8941 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9001 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9061 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9361 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9421 cgattcatta atgcagctgg cacgacaggt ttccc
//
