LOCUS       VB221207-1240mxj        6166 bp    DNA     circular SYN 04-JAN-2024
DEFINITION  
ACCESSION   VB221207-1240mxj
VERSION     VB221207-1240mxj
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..6166
     ITR             1..130
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional
                     equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /note="color: #d05c0a; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="6d343a478711450d8e5c28ceb27e5b84"
     Promoter        158..1494
                     /application_notes="Tissue specificity: Brain. Cell type
                     specificity: Pyramidal neurons."
                     /description="Mouse alpha-calcium-calmodulin dependent
                     kinase II promoter (1.3 kb)"
                     /label="Camk2a(short)"
                     /note="color: #fd3434; direction: RIGHT"
                     /official_designation="Camk2a(short)"
                     /uuid="4830096a4172400ebb45e5a6cfb7708f"
     Miscellaneous   1519..1524
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #46c6ef; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             1525..2202
                     /application_notes="Fast maturation but lower brightness
                     compared to its predecessor DsRed."
                     /description="Monomeric red fluorescent protein 1; variant
                     of DsRed generated by mutagenesis"
                     /full_name="Monomeric red fluorescent protein 1"
                     /label="mRFP1"
                     /note="color: #8ccc27; direction: RIGHT"
                     /official_designation="mRFP1"
                     /uuid="525b71f68daa45c4b97f56c205f5802d"
     Miscellaneous   2227..2354
                     /application_notes="Allows to form mature shRNA and trigger
                     knockdown."
                     /description="Human miR30 5' context with several bases
                     mutation"
                     /label="5' miR-30E"
                     /note="color: #eda481; direction: RIGHT"
                     /official_designation="5' miR-30E"
                     /uuid="968ec937b7c042b39ce821800e82b47a"
     shRNA           2355..2417
                     /application_notes=""
                     /label="{Letm1}"
                     /note="color: #da3bb5; direction: RIGHT"
                     /official_designation="{Letm1}"
                     /uuid="6354d7043712437c9ef27d53cb0dc379"
     Miscellaneous   2418..2547
                     /application_notes="Allows to form mature shRNA and trigger
                     knockdown."
                     /description="Human miR30 3' context with several bases
                     mutation"
                     /label="3' miR-30E"
                     /note="color: #e4b930; direction: RIGHT"
                     /official_designation="5' miR-30E"
                     /uuid="f97a9ba90ef446f196f79c68bc71797e"
     Miscellaneous   2575..3172
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #7a00b5; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     PolyA_signal    3203..3410
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Bovine growth hormone polyadenylation signal"
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /note="color: #05696d; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3418..3547)
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /note="color: #f54c6b; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             4475..5335
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     Rep_origin      5506..6094
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgccc ttaattaaca ttatggcctt
      181 aggtcacttc atctccatgg ggttcttctt ctgattttct agaaaatgag atgggggtgc
      241 agagagcttc ctcagtgacc tgcccagggt cacatcagaa atgtcagagc tagaacttga
      301 actcagatta ctaatcttaa attccatgcc ttgggggcat gcaagtacga tatacagaag
      361 gagtgaactc attagggcag atgaccaatg agtttaggaa agaagagtcc agggcagggt
      421 acatctacac cacccgccca gccctgggtg agtccagcca cgttcacctc attatagttg
      481 cctctctcca gtcctacctt gacgggaagc acaagcagaa actgggacag gagccccagg
      541 agaccaaatc ttcatggtcc ctctgggagg atgggtgggg agagctgtgg cagaggcctc
      601 aggaggggcc ctgctgctca gtggtgacag ataggggtga gaaagcagac agagtcattc
      661 cgtcagcatt ctgggtctgt ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc
      721 acaatggcta aaaagcaggg agagctggaa agaaacaagg acagagacag aggccaagtc
      781 aaccagacca attcccagag gaagcaaaga aaccattaca gagactacaa gggggaaggg
      841 aaggagagat gaattagctt cccctgtaaa ccttagaacc cagctgttgc cagggcaacg
      901 gggcaatacc tgtctcttca gaggagatga agttgccagg gtaactacat cctgtctttc
      961 tcaaggacca tcccagaatg tggcacccac tagccgttac catagcaact gcctctttgc
     1021 cccacttaat cccatcccgt ctgttaaaag ggccctatag ttggaggtgg gggaggtagg
     1081 aagagcgatg atcacttgtg gactaagttt gttcgcatcc ccttctccaa ccccctcagt
     1141 acatcaccct gggggaacag ggtccacttg ctcctgggcc cacacagtcc tgcagtattg
     1201 tgtatataag gccagggcaa agaggagcag gttttaaagt gaaaggcagg caggtgttgg
     1261 ggaggcagtt accggggcaa cgggaacagg gcgtttcgga ggtggttgcc atggggacct
     1321 ggatgctgac gaaggctcgc gaggctgtga gcagccacag tgccctgctc agaagcccca
     1381 agctcgtcag tcaagccggt tctccgtttg cactcaggag cacgggcagg cgagtggccc
     1441 ctagttctgg gggcagctct agagcggggg atccactagt tctagagcgg ccgccaagtt
     1501 tgtacaaaaa agcaggctgc caccatggcc tcctccgagg acgtcatcaa ggagttcatg
     1561 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1621 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     1681 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac
     1741 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1801 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1861 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1921 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc
     1981 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac
     2041 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc
     2101 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     2161 cagtacgagc gcgccgaggg ccgccactcc accggcgcct aaacccagct ttcttgtaca
     2221 aagtggtgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
     2281 cacttgctgg gattacttcg acttcttaac ccaacagaag gctcgagaag gtatattgct
     2341 gttgacagtg agcgctgatg ataagctgat ttctgatagt gaagccacag atgtatcaga
     2401 aatcagctta tcatcattgc ctactgcctc ggacttcaag gggctagaat tcgagcaatt
     2461 atcttgttta ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa
     2521 ttaaaatggt ataaattaaa tcactttcaa ctttattata catagttgga attccgataa
     2581 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2641 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2701 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2761 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2821 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2881 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2941 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3001 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3061 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3121 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcct
     3181 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3241 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3301 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3361 caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga gggccgcagg
     3421 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3481 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3541 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3601 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3661 ggcgggggtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3721 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3781 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3841 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3901 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3961 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4021 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4081 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4141 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4201 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4261 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4321 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4381 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4441 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4501 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4561 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4621 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4681 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4741 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4801 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4861 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4921 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4981 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5041 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5101 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5161 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc
     5221 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5281 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5341 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5401 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5461 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5521 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5581 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5641 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5701 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5761 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5821 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5881 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5941 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6001 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6061 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6121 gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcag
//
