LOCUS       Dual-EX-SMN2        9421 bp DNA     circular SYN 09-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 4, 2024 from SnapGene 7.1.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    304..358
                     /label=SMN2-Exon7
     misc_feature    559..669
                     /label=SMN-Exon6
     CDS             complement(583..648)
                     /codon_start=1
                     /label=3xFLAG
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    complement(692..1154)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(1286..1969)
                     /codon_start=1
                     /label=(3-F)Tyr-EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGI"
     intron          complement(2036..3052)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        complement(3053..3328)
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        complement(3330..3709)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        3740..3844
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             3845..4705
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4876..5464
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             5647..5701
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQ"
     polyA_signal    complement(5703..5837)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(5857..5992)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(6016..6038)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             6045..6084
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
                     /translation="AGTTGFPTGKRAV"
     protein_bind    6097..6118
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        6133..6163
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    6171..6187
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(6572..6627)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(6698..6772)
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.4"
                     /note="/other=CDS"
                     /translation="FTPQVQAAYQKVVAGVANALAHKYH"
     promoter        6831..6849
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     polyA_signal    complement(6945..7056)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     misc_feature    complement(7069..7085)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.8"
                     /note="/other=misc_feature"
     misc_feature    complement(7089..7677)
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     intron          complement(7686..7818)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             complement(7841..9265)
                     /codon_start=1
                     /label=tdTomato
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="SKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLV
                     TVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLK
                     GEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERS
                     EGRHHLFLYGMDELYK"
     misc_feature    9269..9280
                     /label=SMN-Exon8
ORIGIN
        1 aaaaaaaagt ctgctggtct gcctactagt gatataaaat ggcatcatat cctaaagctc
       61 tttattgtga aagtatgttt cttccacata accaaccagt taagtatgag aattctagta
      121 gggatctaga ttaacctttt atctaatagt tttggcatca aaattcttta atattgattg
      181 ttttacatta acctttcaac tttttaacat ctgaactttt taaatgttca aaaacatttg
      241 ttttccacaa accataaagt tttacaaaag taagattcac tttcataatg ctggcagact
      301 tactccttaa tttaaggaat gctgagcacc ttccttcttt ttgattttgt ctaaaaccct
      361 gtaaggaaaa taaaggaagt taaaaaaaat agctatatag acatagatag ctatatatag
      421 atagctttat atggatgtta aaaagtattt tgtttcacaa gacattttac ttattttatt
      481 caacaaaata tgatcagcaa aacaaagtca cataactaca aaaaaattgt caggaaaaga
      541 tgctgagtga ttacttacca tataatagcc agtatgatag cccttgtcat cgtcatcctt
      601 gtaatcgatg tcatgatctt tataatcacc gtcatggtct ttgtagtcgg gaggtggtgg
      661 gggaattatg ctagccatgg ttgtggccat attatcatcg tgtttttcaa aggaaaacca
      721 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
      781 atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca
      841 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
      901 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      961 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
     1021 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
     1081 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
     1141 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
     1201 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
     1261 aggggcggaa tttctagatc cggtggatcc cggcggcggt cacgaactcc agcaggacca
     1321 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     1381 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     1441 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     1501 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     1561 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     1621 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     1681 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     1741 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     1801 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     1861 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     1921 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtac
     1981 cgaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta
     2041 ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc
     2101 agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc
     2161 cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc
     2221 ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc
     2281 ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct
     2341 cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc
     2401 tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc
     2461 ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg
     2521 tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg
     2581 ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc
     2641 gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg
     2701 cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac
     2761 agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac
     2821 cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca
     2881 gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag
     2941 ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc
     3001 gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc
     3061 gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc
     3121 ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct
     3181 cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccgcccccat cgctgcacaa
     3241 aataattaaa aaataaataa atacaaaatt gggggtgggg agggggggga gatggggaga
     3301 gtgaagcaga acgtggggct cacctcgacc atggtaatag cgatgactaa tacgtagatg
     3361 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     3421 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     3481 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     3541 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     3601 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     3661 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     3721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4981 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5641 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5701 cggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5761 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5821 gctgcaataa acaagttagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     5881 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     5941 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     6001 ttaggggcgg gactatggtt gctgactaat tgagatgcgg atccgctggc acgacaggtt
     6061 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg ggctgcaggt
     6241 cgagggatct ccataagaga agagggacag ctatgactgg gagtagtcag gagaggagga
     6301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
     6361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
     6421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg
     6481 cagccagcat atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta
     6541 aatgatttgc cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta
     6601 ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga
     6661 tgtccccata atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat
     6721 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattagcgga
     6781 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6841 cactaaaggg aacaaaagct gggctattgt cttcccaatc ctcccccttg ctgtcctgcc
     6901 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
     6961 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
     7021 ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcgt accgagctcg
     7081 aattccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     7141 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     7201 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     7261 cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag
     7321 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     7381 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     7441 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     7501 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     7561 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     7621 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc
     7681 gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa
     7741 acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct
     7801 tgtaaccttg atacttacct gcggcctcga gttacttaag ctacttgtac agctcgtcca
     7861 tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
     7921 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
     7981 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg ccgccgtcct
     8041 tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
     8101 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt
     8161 tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg
     8221 tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     8281 ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc
     8341 cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
     8401 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     8461 cgaactcgtg gccgttcatg gagccctcca tgcgcacctt gaagcgcatg aactctttga
     8521 tgacggccat gttgttgtcc tcggaggagg cggtgccgga gctgccgctg ccggtgctgc
     8581 cggtgccatg ccccaggaac aggtggtggc ggccctcgga gcgctcgtac tgttccacga
     8641 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt gtccacgtag tagtagccgg
     8701 gcagttgcac gggcttcttg gccatgtaga tggtcttgaa ctccaccagg tagtggccgc
     8761 cgtccttcag cttcagggcc tggtggatct cgcccttcag cacgccgtcg cgggggtaca
     8821 ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggggg
     8881 ggaagttggt gccgcgcatc ttcaccttgt agatcagcgt gccgtcctgc agggaggagt
     8941 cctgggtcac ggtcaccaga ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
     9001 cctcggggaa ggacagcttc ttgtaatcgg ggatgtcggc ggggtgcttc acgtacgcct
     9061 tggagccgta catgaactgg ggggacagga tgtcccaggc gaagggcagg gggccgccct
     9121 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
     9181 cgatctcgaa ctcgtggccg ttcatggagc cctccatgcg caccttgaag cgcatgaact
     9241 ctttgatgac ctcctcgccc ttgctagctg ccagcatttc ctgcaaatga gaaattagaa
     9301 ccagaggctt gacgaattcc agttaaacca tgtcctctgt ggacaccagt taaacttgac
     9361 tagagcactt catatgtcag agtgtacagt gcagtatgcc taggttatcc catatcacaa
     9421 t
//
