LOCUS       pMIA4.721_Ts1c_5        6864 bp DNA     circular SYN 05-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Matias
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2024 from SnapGene 7.1.0
            https://www.snapgene.com
COMMENT     sequence confirmed by nanopore 
            N2314441_30-920089658_Pacbio_2023-09-13
COMMENT     
FEATURES             Location/Qualifiers
     source          1..6864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1206..1878
                     /label=pUC
     rep_origin      1232..1819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2010..2050
                     /label=Ts1 5' into 4.721_fwd
     misc_feature    2030..2338
                     /label=safe coordinates
     variation       2031
                     /replace="C/T"
                     /db_xref="dbSNP:rs72997010"
     variation       2035
                     /replace="T/C"
                     /db_xref="dbSNP:rs769575873"
     variation       2038
                     /replace="C/T"
                     /db_xref="dbSNP:rs972138577"
     variation       2045
                     /replace="A/G"
                     /db_xref="dbSNP:rs1213843591"
     variation       2048
                     /replace="A/G"
                     /db_xref="dbSNP:rs1026102119"
     variation       2053
                     /replace="C/T"
                     /db_xref="dbSNP:rs959763831"
     variation       2059
                     /replace="T/G"
                     /db_xref="dbSNP:rs774717030"
     variation       2060
                     /replace="G/A"
                     /db_xref="dbSNP:rs1202893300"
     variation       2061
                     /replace="G/A"
                     /db_xref="dbSNP:rs1235941628"
     variation       2066
                     /replace="T/C"
                     /db_xref="dbSNP:rs143412571"
     variation       2068
                     /replace="T/C"
                     /db_xref="dbSNP:rs539936284"
     variation       2069
                     /replace="A/G"
                     /db_xref="dbSNP:rs970600659"
     variation       2074
                     /replace="A/T"
                     /db_xref="dbSNP:rs1557717313"
     variation       2075
                     /replace="G/T"
                     /db_xref="dbSNP:rs1384418550"
     variation       2080
                     /replace="A/T"
                     /db_xref="dbSNP:rs1341746904"
     variation       2088
                     /replace="A/G"
                     /db_xref="dbSNP:rs1352026910"
     variation       2097
                     /replace="T/G"
                     /db_xref="dbSNP:rs536382019"
     variation       2101
                     /replace="C/T"
                     /db_xref="dbSNP:rs1398236120"
     variation       2105
                     /replace="G/T"
                     /db_xref="dbSNP:rs926157880"
     variation       2110
                     /replace="G/A"
                     /db_xref="dbSNP:rs934716382"
     variation       2111
                     /replace="G/A"
                     /db_xref="dbSNP:rs1021889826"
     variation       2113
                     /replace="A/G"
                     /db_xref="dbSNP:rs1471523979"
     variation       2120..2122
                     /replace="GGG/GGGG"
                     /db_xref="dbSNP:rs35108382"
     variation       2128
                     /replace="C/G"
                     /db_xref="dbSNP:rs1053037560"
     variation       2136
                     /replace="G/T"
                     /db_xref="dbSNP:rs1391386913"
     variation       2138
                     /replace="A/G"
                     /db_xref="dbSNP:rs985435773"
     variation       2141
                     /replace="G/A"
                     /db_xref="dbSNP:rs1478074002"
     variation       2142..2145
                     /replace="TTTT/TTT"
                     /db_xref="dbSNP:rs912557849"
     variation       2146
                     /replace="A/T"
                     /db_xref="dbSNP:rs922714603"
     variation       2147
                     /replace="G/C"
                     /db_xref="dbSNP:rs1486377238"
     variation       2157
                     /replace="A/G/T"
                     /db_xref="dbSNP:rs560721775"
     variation       2173
                     /replace="G/T"
                     /db_xref="dbSNP:rs1199494203"
     variation       2174
                     /replace="A/T"
                     /db_xref="dbSNP:rs1049773516"
     variation       2184
                     /replace="C/A"
                     /db_xref="dbSNP:rs1254618182"
     variation       2189
                     /replace="A/G"
                     /db_xref="dbSNP:rs767878579"
     variation       2190
                     /replace="T/A"
                     /db_xref="dbSNP:rs1447876383"
     variation       2193
                     /replace="G/A"
                     /db_xref="dbSNP:rs972773585"
     variation       2197
                     /replace="G/A"
                     /db_xref="dbSNP:rs1278156348"
     variation       2203
                     /replace="G/A"
                     /db_xref="dbSNP:rs528637274"
     variation       2204
                     /replace="A/T"
                     /db_xref="dbSNP:rs1007766544"
     variation       2219
                     /replace="G/A"
                     /db_xref="dbSNP:rs1037664325"
     variation       2221
                     /replace="A/T"
                     /db_xref="dbSNP:rs773239351"
     variation       2225
                     /replace="A/G"
                     /db_xref="dbSNP:rs866143979"
     variation       2230
                     /replace="G/A"
                     /db_xref="dbSNP:rs1334979276"
     variation       2231
                     /replace="G/A"
                     /db_xref="dbSNP:rs1244179438"
     variation       2239
                     /replace="T/C"
                     /db_xref="dbSNP:rs755565971"
     variation       2243
                     /replace="A/G"
                     /db_xref="dbSNP:rs1400351560"
     variation       2250
                     /replace="A/C"
                     /db_xref="dbSNP:rs1299601970"
     variation       2251
                     /replace="A/G"
                     /db_xref="dbSNP:rs1465973894"
     variation       2253
                     /replace="C/T"
                     /db_xref="dbSNP:rs1399388100"
     variation       2255
                     /replace="T/C"
                     /db_xref="dbSNP:rs1168417390"
     variation       2256
                     /replace="A/C"
                     /db_xref="dbSNP:rs1476168371"
     variation       2257
                     /replace="G/A"
                     /db_xref="dbSNP:rs1368539884"
     variation       2274
                     /replace="A/G"
                     /db_xref="dbSNP:rs1025965634"
     variation       2276
                     /replace="A/G"
                     /db_xref="dbSNP:rs72997012"
     variation       2280
                     /replace="A/G"
                     /db_xref="dbSNP:rs753502069"
     variation       2292
                     /replace="A/G"
                     /db_xref="dbSNP:rs1014025299"
     variation       2293
                     /replace="T/C"
                     /db_xref="dbSNP:rs1023101129"
     variation       2294
                     /replace="G/A"
                     /db_xref="dbSNP:rs1359124568"
     variation       2298
                     /replace="T/C"
                     /db_xref="dbSNP:rs1213153093"
     variation       2304..2305
                     /replace="AA/A"
                     /db_xref="dbSNP:rs1557717357"
     variation       2314
                     /replace="T/C"
                     /db_xref="dbSNP:rs565412834"
     variation       2315
                     /replace="G/A"
                     /db_xref="dbSNP:rs139210678"
     variation       2317
                     /replace="A/G"
                     /db_xref="dbSNP:rs1570879462"
     variation       2318
                     /replace="T/A"
                     /db_xref="dbSNP:rs1359144121"
     primer_bind     complement(2319..2358)
                     /label=Ts1 5' into 4.721_rev
     variation       2320
                     /replace="A/G"
                     /db_xref="dbSNP:rs1284795129"
     variation       2322
                     /replace="T/A"
                     /db_xref="dbSNP:rs979334979"
     variation       2324
                     /replace="C/A/G/T"
                     /db_xref="dbSNP:rs926256841"
     variation       2325
                     /replace="G/A"
                     /db_xref="dbSNP:rs762632829"
     variation       2331
                     /replace="T/C/G"
                     /db_xref="dbSNP:rs956472675"
     misc_feature    complement(2336..2338)
                     /label=430rev
                     /note="MIT-Specificity score: 76, Efficiency Doench2016 = 
                     69, Efficiency Moreno-Mateos = 62, guide sequence: 
                     GCACCTGGAGACACTACCAA, full details/primers at 
                     http://crispor.org/crispor.py?batchId=gB9qS97ti7j5ZndJFYdV"
     variation       2336
                     /replace="T/G"
                     /db_xref="dbSNP:rs989001645"
     promoter        2353..4068
                     /label=CAGG
     enhancer        2353..2732
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2734..3004
                     /label=chicken beta-actin promoter
     intron          3005..4019
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             3005..3097
                     /gene="exon 1"
                     /product="Chicken b-actin splice donor from exon 1"
                     /label=exon 1
                     /note="/SECDrawAs=Gene"
     CDS             3098..3658
                     /gene="(null)"
                     /product="Beta actin intron 1 (lacking 33 most 3' nts)"
                     /label=(null)
                     /note="/SECDrawAs=Gene"
     CDS             3660..3969
                     /gene="(null)"
                     /product="Beta actin intron 1 (lacking 33 most 3' nts)"
                     /label=(null)
                     /note="/SECDrawAs=Gene"
     misc_RNA        complement(3849..3868)
                     /label=cas9 I off94
     misc_RNA        3925..3944
                     /label=Nm Cas9 off82
     misc_RNA        3948..3967
                     /label=cas9 II off91
     CDS             3979..4019
                     /gene="(null)"
                     /product="Rabbit beta-globin intron (3' part of)"
                     /label=(null)
                     /note="/SECDrawAs=Gene"
     CDS             4020..4068
                     /gene="(null)"
                     /product="rabbit b-globin 3rd exon (5' part of)"
                     /label=(null)
                     /note="/SECDrawAs=Gene"
     unsure          4062..4081
                     /label=cas12a III off97
     misc_RNA        complement(4063..4085)
                     /label=cas12a II off95
     unsure          4063..4082
                     /label=cas12a IV off95
     unsure          4065..4084
                     /label=cas12a I off95
     primer_bind     4170..4188
                     /label=Ts1 5' into 4.721_fwd
     primer_bind     complement(4202..4218)
                     /label=Fragment.REV
     protein_bind    join(4204..4228,4229..4230,4231..4255)
                     /label=BxbI attP - CT
                     /label=BxbI attP
                     /bound_moiety="BxbI integrase"
                     /note="attP site for the BxbI integrase (Ghosh, P et.al.; 
                     2003)
                     central di-nucleotide mutated to cT
                     "
     primer_bind     4237..4255
                     /label=Fragment.FORa
     primer_bind     4239..4279
                     /label=Fragment.FOR
     CDS             4268..5506
                     /codon_start=1
                     /label=2xFKBP12-Casp9
                     /translation="MLEGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDR
                     NKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDV
                     ELLKLESGGGSGVDGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRT
                     RTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELARQDHGALDCCVVVILSHG
                     CQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFE
                     VASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRD
                     PKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKT
                     SVDYPYDVPDYALD"
     CDS             4277..4597
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             5474..5500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     Site            5507..5572
                     /label=gsgP2A
                     /site_type="cleavage site"
     CDS             5516..5572
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5573..5971
                     /label=Bsd
     primer_bind     complement(5951..5995)
                     /label=Fragment.REV
     protein_bind    join(5981..6005,6006..6007,6008..6032)
                     /label=BxbI attP - GT
                     /label=Bsxb1 attP
                     /bound_moiety="BxbI integrase"
                     /note="attP site for the BxbI integrase (Ghosh, P et.al.; 
                     2003)"
     primer_bind     6014..6056
                     /label=Fragment.FORa
     primer_bind     6016..6032
                     /label=Fragment.FOR
     polyA_site      6039..6235
                     /label=SV40
     polyA_signal    6063..6144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6213..6243)
                     /label=Fragment.REVa
     primer_bind     6223..6262
                     /label=Ts1 3' into  4.721_fwd
     misc_feature    6243..6361
                     /label=safe coordinates
     misc_feature    complement(6243..6259)
                     /label=430rev
                     /note="MIT-Specificity score: 76, Efficiency Doench2016 = 
                     69, Efficiency Moreno-Mateos = 62, guide sequence: 
                     GCACCTGGAGACACTACCAA, full details/primers at 
                     http://crispor.org/crispor.py?batchId=gB9qS97ti7j5ZndJFYdV"
     variation       6243
                     /replace="G/A"
                     /db_xref="dbSNP:rs1440145693"
     variation       6246
                     /replace="G/C"
                     /db_xref="dbSNP:rs947916927"
     variation       6254
                     /replace="A/G"
                     /db_xref="dbSNP:rs1284954199"
     variation       6256
                     /replace="G/C/T"
                     /db_xref="dbSNP:rs922620286"
     variation       6257
                     /replace="T/C"
                     /db_xref="dbSNP:rs1162060338"
     variation       6258
                     /replace="G/T"
                     /db_xref="dbSNP:rs933959722"
     variation       6261
                     /replace="A/C"
                     /db_xref="dbSNP:rs1365131886"
     variation       6263
                     /replace="G/A"
                     /db_xref="dbSNP:rs1473765934"
     variation       6264
                     /replace="A/G/T"
                     /db_xref="dbSNP:rs72997013"
     variation       6271
                     /replace="G/A"
                     /db_xref="dbSNP:rs865919035"
     variation       6283
                     /replace="A/G"
                     /db_xref="dbSNP:rs567658084"
     variation       6287
                     /replace="G/C"
                     /db_xref="dbSNP:rs528094596"
     variation       6288
                     /replace="A/G"
                     /db_xref="dbSNP:rs1177171923"
     variation       6290
                     /replace="G/A"
                     /db_xref="dbSNP:rs1465951062"
     variation       6292
                     /replace="G/C"
                     /db_xref="dbSNP:rs1291259228"
     variation       6293
                     /replace="T/C"
                     /db_xref="dbSNP:rs943251838"
     variation       6298
                     /replace="C/A"
                     /db_xref="dbSNP:rs1354306253"
     variation       6299
                     /replace="C/G"
                     /db_xref="dbSNP:rs13375311"
     variation       6305
                     /replace="C/T"
                     /db_xref="dbSNP:rs1173021771"
     variation       6306..6308
                     /replace="TTT/TT"
                     /db_xref="dbSNP:rs1241140095"
     variation       6322
                     /replace="T/C/G"
                     /db_xref="dbSNP:rs1376519528"
     variation       6325
                     /replace="C/T"
                     /db_xref="dbSNP:rs1306737882"
     variation       6328
                     /replace="G/A"
                     /db_xref="dbSNP:rs1404217035"
     variation       6329
                     /replace="T/A"
                     /db_xref="dbSNP:rs1037924457"
     variation       6331
                     /replace="T/C"
                     /db_xref="dbSNP:rs1557717391"
     variation       6341
                     /replace="T/C"
                     /db_xref="dbSNP:rs899104071"
     variation       6353
                     /replace="G/A"
                     /db_xref="dbSNP:rs993432354"
     variation       6357
                     /replace="T/C"
                     /db_xref="dbSNP:rs1385058421"
     variation       6365
                     /replace="A/T"
                     /db_xref="dbSNP:rs536793192"
     variation       6369
                     /replace="A/G"
                     /db_xref="dbSNP:rs1386455810"
     variation       6375
                     /replace="A/G/T"
                     /db_xref="dbSNP:rs576431723"
     variation       6395
                     /replace="C/T"
                     /db_xref="dbSNP:rs895488952"
     variation       6396
                     /replace="A/C"
                     /db_xref="dbSNP:rs1157188436"
     variation       6407
                     /replace="G/T"
                     /db_xref="dbSNP:rs1479221552"
     variation       6408
                     /replace="G/T"
                     /db_xref="dbSNP:rs546735118"
     variation       6411
                     /replace="T/C"
                     /db_xref="dbSNP:rs745629900"
     primer_bind     complement(6417..6467)
                     /label=Ts1 3' into  4.721_rev
     variation       6418
                     /replace="A/C"
                     /db_xref="dbSNP:rs1450458995"
     variation       6426
                     /replace="A/G"
                     /db_xref="dbSNP:rs954331201"
     variation       6427
                     /replace="T/C"
                     /db_xref="dbSNP:rs1217457873"
     variation       6434
                     /replace="G/T"
                     /db_xref="dbSNP:rs1353177460"
     variation       6442
                     /replace="A/G"
                     /db_xref="dbSNP:rs758282678"
     variation       6443
                     /replace="T/C"
                     /db_xref="dbSNP:rs1220696286"
     primer_bind     complement(6447..6467)
                     /label=Fragment.REVa
     primer_bind     6584..6603
                     /label=Ts1 5' into 4.721_fwd
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggcctttt gctcacatgc tgggcccagc cggccagatc tgagctcgcg gccgcgatga
     1921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgg ccagttgcaa
     2041 aaagagaatc tgcttgcatg gagtatgtaa aaaagtcata ttcttggaaa gtagaatgga
     2101 cattgccagg ggaggaagtg ggaagttcaa tggatgcaga gttttagttt tgcaagatga
     2161 aaagttccta gggatctatt gcacaacaat gtgcatgtag ttgacactac tgaactgtgt
     2221 attgaaaaag ggttaagatt gcaaaaatca atcttaggca atttgatttt tcaagacaca
     2281 gacatctaca aatgttatta ttgaagaaac caatgcatca ttacggatta tgcctttgca
     2341 tcatcgtcga tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2401 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2461 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2521 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2581 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2641 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2701 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
     2761 ctctccccat ctccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     2821 ttgtgcagcg atgggggcgg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     2881 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     2941 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3001 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3061 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3121 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3181 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3241 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3301 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3361 cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt
     3421 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     3481 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     3541 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     3601 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     3661 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     3721 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     3781 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     3841 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     3901 ccgcgggggg acggctgcct tcggggggac ggggcagggc ggggttcggc ttctggcgtg
     3961 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4021 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctcgagga
     4081 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4141 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttaa ttcccgggga
     4201 tccgtggttt gtctggtcaa ccaccgcgct ctcagtggtg tacggtacaa acccacacgt
     4261 ggccaccatg ctcgagggag tgcaggtgga gactatctcc ccaggagacg ggcgcacctt
     4321 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa
     4381 agttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
     4441 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
     4501 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
     4561 cactctcgtc ttcgatgtgg agcttctaaa actggaatct ggcggtggat ccggagtcga
     4621 cggatttggt gatgtcggtg ctcttgagag tttgagggga aatgcagatt tggcttacat
     4681 cctgagcatg gagccctgtg gccactgcct cattatcaac aatgtgaact tctgccgtga
     4741 gtccgggctc cgcacccgca ctggctccaa catcgactgt gagaagttgc ggcgtcgctt
     4801 ctcctcgctg catttcatgg tggaggtgaa gggcgacctg actgccaaga aaatggtgct
     4861 ggctttgctg gagctggcgc ggcaggacca cggtgctctg gactgctgcg tggtggtcat
     4921 tctctctcac ggctgtcagg ccagccacct gcagttccca ggggctgtct acggcacaga
     4981 tggatgccct gtgtcggtcg agaagattgt gaacatcttc aatgggacca gctgccccag
     5041 cctgggaggg aagcccaagc tctttttcat ccaggcctgt ggtggggagc agaaagacca
     5101 tgggtttgag gtggcctcca cttcccctga agacgagtcc cctggcagta accccgagcc
     5161 agatgccacc ccgttccagg aaggtttgag gaccttcgac cagctggacg ccatatctag
     5221 tttgcccaca cccagtgaca tctttgtgtc ctactctact ttcccaggtt ttgtttcctg
     5281 gagggacccc aagagtggct cctggtacgt tgagaccctg gacgacatct ttgagcagtg
     5341 ggctcactct gaagacctgc agtccctcct gcttagggtc gctaatgctg tttcggtgaa
     5401 agggatttat aaacagatgc ctggttgctt taatttcctc cggaaaaaac ttttctttaa
     5461 aacatcagtc gactatccgt acgacgtacc agactacgca ctcgacggaa gcggagccac
     5521 taacttctcc ctgttgaaac aagcagggga tgtcgaagag aatcccgggc caatggccaa
     5581 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     5641 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     5701 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     5761 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     5821 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     5881 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     5941 gctgccctct ggttatgtgt gggagggcta ataaggtacc gtggtttgtc tggtcaacca
     6001 ccgcggtctc agtggtgtac ggtacaaacc caaccggtgt ttggacaaac cacaactaga
     6061 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6121 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     6181 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaacctg
     6241 cagtagtgtc tccaggtgca aggaataaca gaaataaaag tcacatgatg tgtgaacccc
     6301 agagctttgg ggagactcct ataccaggtc tgaagtgacc taataggaag tagctgttat
     6361 tcatagtcaa tgccatatta atgaagagaa tgaacaacct caaaatggtc taatatcatg
     6421 actttatcta actgtatcta aatacactgc aggcatgcaa gcttggcact ggccgtcgtt
     6481 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6541 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6601 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     6661 ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6721 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6781 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6841 caccgtcatc accgaaacgc gcga
//
