LOCUS       Exported               10559 bp ds-DNA     circular SYN 04-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 5, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..197
                     /label=AmpR
     CDS             198..989
                     /label=AmpR
     rep_origin      1160..1748
                     /label=ori
     misc_feature    1307
                     /label=CtoT mutation in plasmid
     protein_bind    2036..2057
                     /label=CAP binding site
     promoter        2072..2077
                     /label=lac promoter
     promoter        2078..2095
                     /label=lac promoter
     promoter        2096..2102
                     /label=lac promoter
     protein_bind    2110..2126
                     /label=lac operator
     primer_bind     2134..2150
                     /label=M13 rev
     promoter        2171..2189
                     /label=T3 promoter
     misc_feature    2437..2438
                     /label=A deletion in plasmid
     LTR             2444..2624
                     /label=5' LTR (truncated)
     misc_feature    2644
                     /label=CtoT mutation in plasmid
     misc_feature    2665..2667
                     /label=insertion in plasmid
     misc_feature    2671..2796
                     /label=HIV-1 Psi
     misc_feature    3289..3522
                     /label=RRE
     CDS             3707..3751
                     /label=gp41 peptide
     misc_feature    4018..4134
                     /label=cPPT/CTS
     protein_bind    4168..4188
                     /label=attB4
     promoter        4188..5399
                     /label=UbC promoter
     protein_bind    complement(5403..5423)
                     /label=attB5
     misc_feature    5424..5433
                     /label=Kozak sequence
     CDS             5436..6206
                     /label=miniTurbo
     CDS             6213..6254
                     /label=V5 tag
     protein_bind    6255..6279
                     /label=attB1
     misc_feature    6282..6284
                     /label=HvdLib Fill
     CDS             6285..7379
                     /codon_start=1
                     /label=DK131. STK19 (CCDS34417.1)
                     /translation="MQKWFSAFDDAIIQRQWRANPSRGGGGVSFTKEVDTNVATGAPPR
                     RQRVPGRACPWREPIRGRRGARPGGGDAGGTPGETVRHCSAPEDPIFRFSSLHSYPFPG
                     TIKSRDMSWKRHHLIPETFGVKRRRKRGPVESDPLRGEPGSARAAVSELMQLFPRGLFE
                     DALPPIVLRSQVYSLVPDRTVADRQLKELQEQGEIRIVQLGFDLDAHGIIFTEDYRTRV
                     LKACDGRPYAGAVQKFLASVLPACGDLSFQQDQMTQTFGFRDSEITHLVNAGVLTVRDA
                     GSWWLAVPGAGRFIKYFVKGRQAVLSMVRKAKYRELLLSELLGRRAPVVVRLGLTYHVH
                     DLIGAQLVDCISTTSGTLLRLPET"
     protein_bind    complement(7381..7390)
                     /label=attB2
     misc_feature    7435..8023
                     /label=WPRE
     CDS             complement(7906..7917)
                     /label=Factor Xa site
     promoter        8037..8536
                     /label=PGK promoter
     CDS             8557..9156
                     /label=PuroR
     LTR             9294..9527
                     /label=3' LTR (Delta-U3)
     misc_feature    9393..9394
                     /label=GCtoAA mutation in plasmid
     polyA_signal    9599..9720
                     /label=SV40 poly(A) signal
     rep_origin      9760..9895
                     /label=SV40 ori
     misc_feature    9899..9900
                     /label=CTTTTGCGTCGA deletion in plasmid
     promoter        complement(9916..9934)
                     /label=T7 promoter
     primer_bind     complement(9944..9960)
                     /label=M13 fwd
     misc_feature    10100..10101
                     /label=CGC deletion in plasmid
     misc_feature    10102..10557
                     /label=f1 ori
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2221 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2281 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     2341 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2401 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2641 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaact ttgtacaaaa aagcaggctc caccatgcaa aagtggtttt
     6301 ctgctttcga tgatgcaatc attcagcgac agtggcgggc aaacccctcc cggggcgggg
     6361 gaggtgtgag cttcacgaag gaggttgaca ccaacgtggc caccggcgcc cctccacgcc
     6421 gccaacgagt ccccgggcgt gcgtgccctt ggagggagcc aatccgcggc cggcgtgggg
     6481 cccggcctgg cggaggtgat gctggaggga cgcccgggga gaccgtacgt cactgctctg
     6541 cgccggaaga ccctattttc aggttctctt ccctccattc ctaccccttc cccggtacca
     6601 taaaatcccg ggatatgagc tggaagaggc atcacctgat cccggagacc tttggagtta
     6661 agaggcggcg gaagcgaggg cctgtggagt cggatcctct tcggggtgag ccagggtcgg
     6721 cgcgcgcggc tgtctcagaa ctcatgcagc tgttcccgcg aggcctgttt gaggacgcgc
     6781 tgccgcccat cgtgctgagg agccaggtgt acagccttgt gcctgacagg accgtggccg
     6841 accggcagct gaaggagctt caagagcagg gggagatcag aatcgtccag ctgggcttcg
     6901 acttggatgc ccatggaatt atcttcactg aggactacag gaccagagtc ctcaaggcct
     6961 gtgatggccg accgtatgct ggggcagtgc agaaatttct agcttcagta cttccagcct
     7021 gtggggacct tagtttccag caggaccaaa tgacacagac ctttggcttc agggactcag
     7081 aaatcacgca tctggtgaat gctggagtcc tcaccgtccg agatgctggg agctggtggc
     7141 tagctgtgcc tggagctggg agattcatca agtactttgt taaagggcgc caggctgtcc
     7201 ttagcatggt ccggaaggca aagtaccggg aactgctcct atcagagctc ctgggccggc
     7261 gggcgcctgt cgtggtgcgg cttggcctca cctaccatgt gcacgacctc attggggccc
     7321 agctagtgga ctgcatctct accacttcag gaaccctcct ccgcctgcca gagacatagg
     7381 acccagcttt cttgtacaaa gttgtgatat ccagcacagt ggcggccgct cgacaatcaa
     7441 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7501 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7561 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7621 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7681 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7741 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7801 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7861 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7921 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7981 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt
     8041 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     8101 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     8161 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     8221 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     8281 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     8341 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     8401 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     8461 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     8521 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     8581 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     8641 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     8701 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8761 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8821 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8881 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8941 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     9001 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     9061 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     9121 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     9181 ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat
     9241 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     9301 gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     9361 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9421 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9481 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9541 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     9601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9721 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     9781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     9901 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     9961 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    10021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    10081 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    10141 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    10201 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    10261 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    10321 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    10381 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    10441 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    10501 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttag
//
