LOCUS       AiP1511                 6145 bp    DNA     circular UNA 25-OCT-2023
DEFINITION  pAAV-AiE2190m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   urn.local.yoav.ben-simon.1df5-gwwkrx0
VERSION     urn.local.yoav.ben-simon.1df5-gwwkrx0
KEYWORDS    .
SOURCE       (AiP1511-pAAV-MGT_E190-minBGpromoter-SYFP2-WPRE-bGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        169..2052
                     /X-Transferred_From="AiE2190m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:7v3-gvrlgbh"">AiE2190m</a>
                     "
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2190m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:7v3-gvrlgbh"">AiE2190m</a>
                     "
                     /label="AiE2190m"
     promoter        2070..2122
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      2157..2166
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             2163..2882
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="98.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="98.19%; Adjusted-Interval-0:0/-3
                     (adjusted to 97.78%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="98.06%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="97.79%; Adjusted-Interval-0:-3/0
                     (adjusted to 97.37%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="96.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /Alternative_Match="94.09%; Adjusted-Interval-0:-44/-3; <a
                     href=""urn:local:yoav.ben-simon:1bu-gl1nwa6"">mTurquoise</
                     a>"
                     /label="SYFP2"
     3'UTR           2917..3162
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    3176..3379
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             3408..3548
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      3640..3946
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        4360..4464
                     /gene="bla"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     CDS             4465..5325
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="99.65%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-3
                     (adjusted to 99.65%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      5473..6140
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gccggcggat taatctgggc
      181 aggaatgtat gctttgcttc tctaacacgg ctcctcgtga agatatggca cagctatgtg
      241 tttggaaaac taagtagaag cagagttggg agggtccagg atttcccctc ggtccccaga
      301 gtacaggact gaagaggctt acactgaagt aagctcagaa gaggtgctat caaggacttc
      361 tcagcccttc actgagggct tgtgtgactc aagtcatggc attgttttgg gagaaatagg
      421 ttgtgtatac aggcaggctg ggtggccaac aagcacgagg gatgtggaga aggccatatg
      481 atctgggaca ttaaggactt tcctgctttt caaagaagca agtcccaacc ccaaaactac
      541 cagtaacctc tcaatttggc atgcagaaag aaaaggcgca agtcaagtaa cctcatcaag
      601 gcacaaggtg ccagtttcta cctcatctct atgtcataaa ccttgaggtc tccagtcact
      661 caagttagcc agcccaaggc ttcctctatc tctactagct ccagcacacc ccctacaata
      721 cctaccacac ttgcaaaata ggtctgcagg cagaattaat cttcatacaa cattcaagta
      781 cctttaatca gcctgtcacc agctcaagtc tccagcttct agcatctttt cactgttccc
      841 ttctggtatc ttcccttgct gcagggccaa gacatttcta ttctcatcct taattgtaac
      901 tgagaagata aactgggagc cttgagacct gatttggagc tgtagacttg tcagtcactg
      961 gaaaattctg agaatcctca atctaagtga agggagcgca cccccaaccc cccccccaac
     1021 acacacacac acacactttg tgtctgttac tttaaatcca tctatgatgc caggatgatt
     1081 ctaccagtgg tttacaactt gaatgcttac atgctgtaga gcatagcaga cagcttcatt
     1141 tctaagtgtg aattctgagt aggtgagatg tctcagcagg tagaagcact tgctatgcaa
     1201 acctgacaga cagcgttcag ttccctgaat ccacaggaga aagcaccagt tcctcagagt
     1261 tgtctgctta cctgcagatg tcacctgcac atgtatggtg cacatgtgct ttcactcata
     1321 tgcacacaca tccatatatg gacaataaca aaatatttta agtaggaata ccattttcta
     1381 aaccaagcaa ttttataaac aactgtctta tgataaagct aatacataag aacctaaagc
     1441 catataaatt ctgtacccct tgaatgtgac ctgcatatac taatcatgaa ggcatctact
     1501 actatctcta ctatgcttaa aagtttatgt atatgtgtgt cttcagttta taataagttg
     1561 atcaaaatga ttcttcctga gatctactca gaaaaactct gatccaatgg atttggaagt
     1621 agagtcctgg aagctgcact gtaacctgtg atgtttgtga aacatttcca taagatcttc
     1681 tttgaattac cttcctgctt actggagagg cttcatcatt cagttaaatg aggctgtcat
     1741 gagttggctg ttggtctctc tttaatgtcc tctggggtct atgaaggtag atacgtgaag
     1801 ttccacagga agcagtttga tggtcatcaa acaagataga tactgaaaga tgggatttag
     1861 tgcctggtga gtgtctgtta tagaacaggg ctctcaccta cctttgactt tcacagtaaa
     1921 ggcttcaaac ttgcaatcat tatttctggt gctacagaga aaaaccaaag gagattcatg
     1981 ttgatgttgc ttctcattgg ctctagacca gctttgcaaa ctcagttttg gccagagtta
     2041 ctccttaatt gtgtgcgccg gcggagctcg ggctgggcat aaaagtcagg gcagagccat
     2101 ctattgctta catttgcttc tgggatccag atctttcgaa gctagcgcta ccggtcgcca
     2161 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2221 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2281 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
     2341 ccctcgtgac caccctgggc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     2401 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2581 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
     2641 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     2701 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2761 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     2821 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     2881 aagtcgacgg cgcgccgcgg ccgcgaattc gatatcataa tcaacctctg gattacaaaa
     2941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3061 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct
     3121 gctggacagg ggctcggctg ttgggcactg acaattccgt ggctcgagag atcttcgact
     3181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3361 gaagacaata gcaggcatga gatctcacgt gcggaccgag cggccgcagg aacccctagt
     3421 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3481 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     3541 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3601 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3781 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     3841 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3901 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     3961 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4021 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     4081 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4141 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4201 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4261 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4321 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4381 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4441 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4501 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4561 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4621 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4681 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4741 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4801 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4861 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4921 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4981 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5041 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5101 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5161 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5221 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5281 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5341 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5401 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5641 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5881 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6061 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6121 ttttgctggc cttttgctca catgt
//
