LOCUS       GenRep_03             10202 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(6370..8019)
                     /note="pLannotate"
                     /label="luciferase"
                     /database="addgene"
                     /identity="99.939"
                     /match_length="99.8185117967332"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3286..4215)
                     /note="pLannotate"
                     /label="hRluc"
                     /database="addgene"
                     /identity="99.892"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             9172..10032
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1335..2129)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(2539..2967)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(8422..8801)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(2196..2525)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(3013..3237)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     promoter        complement(8218..8421)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    1031..1161
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="addgene"
                     /identity="98.496"
                     /match_length="97.08029197080292"
                     /fragment="False"
                     /other="polyA signal"
     promoter        9067..9171
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(8026..8067)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    complement(8956..9008)
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="37.5886524822695"
                     /fragment="True"
                     /other="misc feature"
     promoter        complement(8155..8173)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     LTR             8189..8222
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="addgene"
                     /identity="97.059"
                     /match_length="18.88888888888889"
                     /fragment="True"
                     /other="LTR"
     promoter        complement(2988..3009)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="6.197183098591549"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagcttg gtaccgagct cgtcactgct cgttcttcag
     3301 cacgcgctcc acgaagctct tgatgtactt acccatttca tctggagcgt cctcctggct
     3361 gaagtggagg cccttcacct tcacgaactc ggtgttaggg aacttcttag ctccctcgac
     3421 aatagcgttg gaaaagaacc cagggtcgga ctcgatgaac atcttaggca gatcgtcgct
     3481 ggcccgaagg taggcgttgt agttgcggac aatctggacg acgtcgggct tgcctccctt
     3541 aacgagaggg atctcgcgag gccaggagag ggtaggccgt ctaacctcgc ccttctcctt
     3601 gaatggctcc aggtaggcag cgaactcctc aggctccagt ttccgcatga tcttgcttgg
     3661 gagcatggtc tcgacgaaga agttattctc aagcaccatt ttctcgccct cttcgctctt
     3721 gatcagggcg atatcctcct cgatgtcagg ccactcgtcc caggactcga tcacgtccac
     3781 gacactctca gcatggacga tggccttgat cttgtcttgg tgctcgtagg agtagtgaaa
     3841 ggccagacaa gccccccagt cgtggcccac aaagatgatt ttctttggaa ggttcagcag
     3901 ctcgaaccaa gcggtgaggt acttgtagtg atccaggagg cgatatgagc cattcccgct
     3961 cttgccggac ttacccattc cgatcagatc agggatgatg catctagcca cgggctcgat
     4021 gtgaggcacg acgtgcctcc acaggtagct ggaggcagcg ttaccatgca gaaaaatcac
     4081 ggcgttctcg gcgtgcttct cggaatcata gtagttgatg aaggagtcca gcacgttcat
     4141 ttgcttgcag cgagcccacc actgaggccc agtgatcatg cgtttgcgtt gctcggggtc
     4201 atacaccttg gaagctggtt tatgatggat gttgcctaat gagccacact tggaggtcac
     4261 cttgctcagg tcaactggtt tgtagactat ttgcacctgg agatgagaga ggaggagaga
     4321 gaagaggaaa ggcagagaga gaggaagaca gagagacaaa aaggataaaa tgggattatt
     4381 ttattaaggg tgtaactcaa tgagaagcag ctgtgtaagc ccaggccctt attggacagt
     4441 ggttcccagc tgccatgagg agcacatcct agtcagggtg gaaaaatgtg ttgtcgaaat
     4501 tctgccaacc ctgccccagg ggagctgggg actgtgggac tggagtgtct tcaatccctg
     4561 cagaacaaaa gagcctctgg gaaaaacagg gacccattgt cattgtgcaa gacccagaca
     4621 tttgctcagc aaacgcttaa gtgtaaaaat gtcactccct ttcctgacat gaaattgaca
     4681 gatcacaatc aggcatgaag aaaaaagtga tcaatatgat gagtatgcac agcatctgaa
     4741 aaatcttgct ggaaaggcag gagtggggta tctgctgggc ctcaaagagg ggaggcgtcc
     4801 gctcatcaga acaaacgggt cacctgtcca cagtggagcc actcaacgtc aacctgccca
     4861 ggtgtcgggg gaggaggcgg cagcccagtc tcaggtgcca ccaccttcag cccaacttcc
     4921 aatgggctat ggggctcctg gggacagggc tgggcatgag tggaaggaga ctttgatggg
     4981 tattctcagc ttcccaggca gggtccggag agagggtccg tcatctgccc tattctgtcc
     5041 acacagcctg cgcaggctgg gggacccaga aatgctggga caccccctcc tagaatatga
     5101 ggaaggggct tctgggaaca gtggaccgtg tgggggaatg tggggagccg ggctacattc
     5161 acccagaggt cgcagccaga tcctggaatt ccgatcctga tccggatccc ctacttgctc
     5221 gcaaggatgc ctccactttc ggacttggca gaggtagtct ggggagtgac gggaggttga
     5281 gtcacatccc aggggagctg aagggataga gggctgctct gaaggcatcc cagaagtgtg
     5341 agtgatttcc agcgggcttt acgaagctgg gattgcaggt cctcacttca caaaaggatg
     5401 gcatcgtgag gcctgaaccc cagaggggag cgttcaccag cctgaggtca agtgaaaaat
     5461 gtgttcggcc acactgacga gttagtaata ggaagttccc cggcatctcc actcacacct
     5521 ggctagaccc accggcacat tcccagagaa ctccctgcgg ctgttctcaa gcctgccggg
     5581 catcagaatc acctgtggat ttctcccaga agaatcagct gatcaaaaca gaacccccaa
     5641 tctccccaga atccagagga acccaaccta agcagatggc ccacgagtgg agatgctgac
     5701 ctatgggtgg cagtgtattc tgcccagaag gcaggtgggg aaccactgtg ggtgccctga
     5761 ctatgagagc ctccggccgt ggagacacag ccctgccatc tctgtccgat taacaggggc
     5821 tgaaggggca agagaagagc cagaacgttc tctcgcctga ggagggcact cacagtgtag
     5881 tggagagccc aataaagaac tgaacttcct tgaagagggt ccagagggga ctggggtgtt
     5941 atgcgggggt gctcaggtgc ggggagcccc acacctccat gcacagtccc acgactccac
     6001 gctcaaccgc gcacctccag gcgcagccct acccttccag gcacagccct acccctccag
     6061 gcgcagcccc ccacacctcc acgcgcagcc agccactctc accttcccgc ctcccggctg
     6121 gtgcttcagg ttctcagtgg agccgatctt ggacttgaca ttcttcaggt ctggcatggg
     6181 cacgggggct gtctgcaggc ggctcttggc ggaagacggc gacttgggtg gagtacggac
     6241 cactgccacc ttcttgggct cccgggtggg tggggttgga agggacgggg tgcgggagcg
     6301 gctgctggga gtgcctgggg agccggggct gctgtagccg ctgcgatccc ctgattttgg
     6361 aggttcactc acggcgatct tgccgccttt cttagccttg atcaggatct cgcggatctt
     6421 gcgggcgtcc agcttgccgg tcaggccctt gggcacctcg tccacgaaca ccactccgcc
     6481 gcgcagcttc ttggcggttg tcacctggct ggccacatag tccacgatct ccttctcggt
     6541 catggtcttg ccgtgttcca gcacgacgac ggcggcaggc agctcgccgg catcgtcgtc
     6601 gggcaggccg gccactccgg cgtcgaaaat gttagggtgc tgcagcagga tagactccag
     6661 ctcggctggg gccacctggt agcccttgta cttgatcaga gacttcaggc ggtccacgat
     6721 gaagaagtgc tcgtcctcgt cccagtaggc aatgtcgcca gagtgcagcc agccgtcctt
     6781 gtcgatcagg gcgtttgtgg cctcagggtt attcacgtag ccggacataa tcatagggcc
     6841 gcgcacacac agctcgccgc gctggttcac tcccagggtc ttgccggtgt ccaggtccac
     6901 caccttggcc tcgaagaatg gcaccacctt gcccacggcg ccaggcttgt cgtcgccctc
     6961 tggggtaatc agaatggcgc tggttgtctc ggtcaggccg tagccctggc ggatgcctgg
     7021 cagatgaaag cgcttggcca cggcttcgcc cacctcctta gacagtgggg cgccgccaga
     7081 ggcaatctcg tgcaggttag acaggtcgta cttgtcgatc agggtgctct tagcggagaa
     7141 gctgaacagg gttggcacca gcagggcaga ctgaattttg tagtcttgca ggctgcgcag
     7201 gaacagctcc tcctcgaagc ggtacatcag caccacccga aagccgcaaa tcaggtagcc
     7261 cagggtggtg aacatgccga agccgtggtg gaatggcacc acgctcagaa tagcggtgtc
     7321 ggggatgatc tggttgccga aaatagggtc gcgggcgtga gagaagcgca cacaggcggt
     7381 gcgatgaggc agggccacgc ccttaggcag gccggtagac ccagagctgt tcatgatcag
     7441 ggcaatggtt ttgtccctgt cgaaagactc tggcacgaag tcgtactcgt tgaagccagg
     7501 aggcagatga gatgtcacga atgtgtacat gctctggaag ccctggtagt cggtcttaga
     7561 gtccatgatg atgatcttct ggatgatagg cagcttcttc tgcacgttca ggatcttctg
     7621 caggcccttc ttagacacga acaccacggt aggctgagaa atgcccatgc tgttcagcag
     7681 ctcgcgctcg ttgtaaatgt cgttagcagg ggccacggcc actccgatga acagggcgcc
     7741 cagcactggc atgaagaact gcagagagtt ctcagagcac accacgatgc ggtggttggt
     7801 gttcaggccg tacctcttca tggcctcggc caggcgcaca gacatctcga agtactcggc
     7861 ataggtgatg tccacctcaa tgtgggcatc ggtgaaggca atggtgccag gcaccagggc
     7921 atacctcttc atggccttgt gcagctgctc gccagcggtg ccatcctcca gagggtagaa
     7981 gggagcaggg cccttcttaa tgttcttagc atcggccatg ctgcccgtag aatcgagacc
     8041 gaggagaggg ttagggatag gcttaccgct gcccatggtg gctgcagata tccagcacag
     8101 tggcggccgc tcgagtctag agggcccgtt taaacgctag ccagcttggg tctccctata
     8161 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
     8221 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     8281 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     8341 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     8401 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     8461 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     8521 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     8581 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     8641 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     8701 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     8761 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg
     8821 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
     8881 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
     8941 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     9001 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
     9061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10201 tc
//
