LOCUS       Exported                3453 bp ds-DNA     circular SYN 25-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          74..868
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          869..1123
                     /organism="Escherichia species"
                     /strain="strain"
                     /mol_type="genomic DNA"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..36)
                     /label=MBA645
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     complement(7..36)
                     /label=MBA695
     primer_bind     73..107
                     /label=MBA704
     gene            74..868
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /label=thyA
                     /db_xref="EcoGene:EG11002"
     CDS             74..868
                     /codon_start=1
                     /transl_table=11
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /product="thymidylate synthetase"
                     /function="enzyme; 2'-Deoxyribonucleotide metabolism"
                     /EC_number="2.1.1.45"
                     /label=thyA
                     /note="GO_component: GO:0005737 - cytoplasm; GO_process: 
                     GO:0015949 - nucleobase, nucleoside and nucleotide 
                     interconversion"
                     /db_xref="ASAP:ABE-0009268"
                     /db_xref="UniProtKB/Swiss-Prot:P0A884"
                     /db_xref="EcoGene:EG11002"
                     /experiment="N-terminus verified by Edman degradation: PMID
                     6308660"
                     /protein_id="AAC75866.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     primer_bind     complement(843..868)
                     /label=MBA703
     primer_bind     865..899
                     /label=MBA822
     RBS             869..904
                     /label=RBS (4427)
     primer_bind     869..894
                     /label=AGR822
     CDS             905..1123
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="ODNLECBM_00895"
                     /product="translation initiation factor IF-1 [Escherichia 
                     coli Nissle 1917]"
                     /label=infA
                     /inference="ab initio prediction:same species:2.6"
                     /inference="similar to AA sequence:same species:AID81871.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYI
                     RILTGDKVTVELTPYDLSKGRIVFRSR"
     primer_bind     complement(1100..1128)
                     /label=MBA821
     primer_bind     1123..1154
                     /label=MBA697
     misc_feature    1124..1127
                     /label=BsaI Overlap
     primer_bind     complement(1254..1274)
                     /label=MBA277
     primer_bind     complement(1256..1274)
                     /label=MBA219
     primer_bind     complement(1261..1280)
                     /label=MBA118
     primer_bind     complement(1265..1286)
                     /label=MBA095
     primer_bind     complement(1266..1293)
                     /label=MBA191
     primer_bind     1266..1293
                     /label=MBA190
     primer_bind     complement(1329..1349)
                     /label=JL026
     primer_bind     1330..1369
                     /label=JL027
     primer_bind     complement(1331..1349)
                     /label=MBA075
     misc_feature    1350..2494
                     /label=SpecR
     primer_bind     complement(1409..1426)
                     /label=MBA029
     misc_feature    1484..2494
                     /label=SpecR Orf
     primer_bind     complement(1544..1575)
                     /label=MBA176
     primer_bind     complement(2048..2072)
                     /label=MBA143
     primer_bind     2048..2072
                     /label=MBA144
     primer_bind     2368..2391
                     /label=MBA733
     primer_bind     complement(2468..2514)
                     /label=JL028
     primer_bind     2495..2519
                     /label=MBA216
     primer_bind     2495..2519
                     /label=AGR199
     terminator      2519..2647
                     /label=dbl term
     primer_bind     complement(2522..2544)
                     /label=AGR157
     primer_bind     2594..2620
                     /label=MBA215
     primer_bind     complement(2630..2653)
                     /label=AGR222
     primer_bind     complement(2630..2648)
                     /label=AGR295
     rep_origin      complement(2654..3365)
                     /direction=LEFT
                     /label=p15a
     primer_bind     2654..2677
                     /label=MBA209
     primer_bind     2654..2677
                     /label=AGR221
     primer_bind     complement(2737..2758)
                     /label=AGR208
     primer_bind     complement(2758..2780)
                     /label=MBA800
     primer_bind     complement(3072..3098)
                     /label=MBA183
     primer_bind     3072..3098
                     /label=MBA182
     primer_bind     complement(3072..3096)
                     /label=AGR030
     primer_bind     3072..3096
                     /label=AGR029
     primer_bind     3217..3242
                     /label=045seq2
     primer_bind     3312..3335
                     /label=MBA276
     primer_bind     3316..3335
                     /label=MBA194
     primer_bind     complement(3334..3365)
                     /label=MBA210
     primer_bind     3381..3421
                     /label=47R
     primer_bind     3418..3443
                     /label=ptetF
     primer_bind     complement(3427..3453)
                     /label=MBA549
     primer_bind     complement(join(3430..3453,1..20))
                     /label=MBA147
     primer_bind     complement(3430..3453)
                     /label=MBA189
     primer_bind     complement(3430..3453)
                     /label=MBA161
     primer_bind     complement(3434..3453)
                     /label=MBA764
     primer_bind     join(3451..3453,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcgac cgctatttta gcccatatca
       61 gaggtaacac ctaatgaaac agtatttaga actgatgcaa aaagtgctcg acgaaggcac
      121 acagaaaaac gaccgtaccg gaaccggaac gctttccatt tttggtcatc agatgcgttt
      181 taacctgcaa gatggattcc cgctggtgac aactaaacgt tgccacctgc gttccatcat
      241 ccatgaactg ctgtggtttc tgcagggcga cactaacatt gcttatctac acgaaaacaa
      301 tgtcaccatc tgggacgaat gggccgatga aaacggcgac ctcgggccag tgtatggtaa
      361 acagtggcgc gcctggccaa cgccagatgg tcgtcatatt gaccagatca ctacggtact
      421 gaaccagctg aaaaacgacc cggattcgcg ccgcattatt gtttcagcgt ggaacgtagg
      481 cgaactggat aaaatggcgc tggcaccgtg ccatgcattc ttccagttct atgtggcaga
      541 cggcaaactc tcttgccagc tttatcagcg ctcctgtgac gtcttcctcg gcctgccgtt
      601 caacattgcc agctacgcgt tattggtgca tatgatggcg cagcagtgcg atctggaagt
      661 gggtgatttt gtctggaccg gtggcgacac gcatctgtac agcaaccata tggatcaaac
      721 tcatctgcaa ttaagccgcg aaccgcgtcc gctgccgaag ttgattatca aacgtaaacc
      781 cgaatccatc ttcgactacc gtttcgaaga ctttgagatt gaaggctacg atccgcatcc
      841 gggcattaaa gcgccggtgg ctatctaagg ttcaaattac ggtagtgata ccccagagga
      901 ttagatggcc aaagaagaca atattgaaat gcaaggtacc gttcttgaaa cgttgcctaa
      961 taccatgttc cgcgtagagt tagaaaacgg tcacgtggtt actgcacaca tctccggtaa
     1021 aatgcgcaaa aactacatcc gcatcctgac gggcgacaaa gtgactgttg aactgacccc
     1081 gtacgacctg agcaaaggcc gcattgtctt ccgtagtcgc tgaagcctca aagtaatcaa
     1141 gccctggtaa ctgccagggc ttttttattt catctttgaa tcaacatatt aaaattcatc
     1201 gaatggcatc cttgctaaca gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1261 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1321 gtccaagcga gctctcgaac cccagagtcc cagccaggac agaaatgcct cgacttcgct
     1381 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     1441 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     1501 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1561 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1621 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1681 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1741 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1801 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1861 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1921 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1981 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     2041 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     2101 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     2161 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     2221 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2281 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     2341 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     2401 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     2461 gaaaggcgag atcaccaagg tagtcggcaa ataaggatcc aaactcgagt aaggatctcc
     2521 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2581 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2641 gtttatacct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     2701 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     2761 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     2821 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     2881 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     2941 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     3001 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     3121 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     3181 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     3241 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     3301 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     3361 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcagag
     3421 gctagtgtag atcgctacta gagcgcactg acc
//
