LOCUS       Exported                5093 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          71..1900
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     complement(7..35)
                     /label=MBA695
     RBS             36..70
                     /label=RBS (8455)
     primer_bind     70..91
                     /label=MBA702
     gene            71..1900
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /label=glmS
                     /db_xref="EcoGene:EG10382"
     CDS             71..1900
                     /codon_start=1
                     /transl_table=11
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /product="L-glutamine:D-fructose-6-phosphate 
                     aminotransferase"
                     /function="enzyme; Central intermediary metabolism: Amino 
                     sugars"
                     /EC_number="2.6.1.16"
                     /label=glmS
                     /note="GO_component: GO:0009274 - peptidoglycan-based cell 
                     wall; GO_component: GO:0005737 - cytoplasm; GO_function: 
                     GO:0042280 - cell surface antigen activity, 
                     host-interacting; GO_process: GO:0009243 - O antigen 
                     biosynthetic process; GO_process: GO:0009252 - 
                     peptidoglycan biosynthetic process; GO_process: GO:0046349 
                     - amino sugar biosynthetic process"
                     /db_xref="ASAP:ABE-0012198"
                     /db_xref="UniProtKB/Swiss-Prot:P17169"
                     /db_xref="EcoGene:EG10382"
                     /protein_id="AAC76752.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMTR
                     LRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGIIEN
                     HEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTVIMD
                     SRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRRSVNI
                     FDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQVDLSE
                     LGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYRKSAVRR
                     NSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMTNAGTEIG
                     VASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIEAL
                     AEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPV
                     IVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIF
                     YTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     primer_bind     complement(502..514)
                     /label=AGR222
     primer_bind     complement(502..514)
                     /label=AGR295
     primer_bind     1742..1766
                     /label=MBA1116
     primer_bind     complement(1876..1900)
                     /label=MBA701
     RBS             1901..1935
                     /label=nadE RBS (5392)
     primer_bind     1912..1964
                     /label=MBA1156
     CDS             1936..2763
                     /codon_start=1
                     /label=nadE
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLVL
                     GISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQPD
                     RVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGTDHA
                     AEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDRPSLP
                     DEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     primer_bind     complement(2735..2763)
                     /label=MBA1157
     primer_bind     2763..2794
                     /label=MBA697
     misc_feature    2764..2767
                     /label=BsaI Overlap
     primer_bind     complement(2894..2914)
                     /label=MBA277
     primer_bind     complement(2896..2914)
                     /label=MBA219
     primer_bind     complement(2901..2920)
                     /label=MBA118
     primer_bind     complement(2905..2926)
                     /label=MBA095
     primer_bind     complement(2906..2933)
                     /label=MBA191
     primer_bind     2906..2933
                     /label=MBA190
     primer_bind     complement(2969..2989)
                     /label=JL026
     primer_bind     2970..3009
                     /label=JL027
     primer_bind     complement(2971..2989)
                     /label=MBA075
     misc_feature    2990..4134
                     /label=SpecR
     primer_bind     complement(3049..3066)
                     /label=MBA029
     misc_feature    3124..4134
                     /label=SpecR Orf
     primer_bind     complement(3184..3215)
                     /label=MBA176
     primer_bind     complement(3688..3712)
                     /label=MBA143
     primer_bind     3688..3712
                     /label=MBA144
     primer_bind     complement(4108..4154)
                     /label=JL028
     primer_bind     4135..4159
                     /label=MBA216
     primer_bind     4135..4159
                     /label=AGR199
     terminator      4159..4287
                     /label=dbl term
     primer_bind     complement(4162..4184)
                     /label=AGR157
     primer_bind     4234..4260
                     /label=MBA215
     primer_bind     complement(4270..4293)
                     /label=AGR222
     primer_bind     complement(4270..4288)
                     /label=AGR295
     rep_origin      complement(4294..5005)
                     /direction=LEFT
                     /label=p15a
     primer_bind     4294..4317
                     /label=MBA209
     primer_bind     4294..4317
                     /label=AGR221
     primer_bind     complement(4377..4398)
                     /label=AGR208
     primer_bind     4406..4425
                     /label=MBA1184
     primer_bind     complement(4504..4521)
                     /label=MBA1185
     primer_bind     complement(4712..4738)
                     /label=MBA183
     primer_bind     4712..4738
                     /label=MBA182
     primer_bind     complement(4712..4736)
                     /label=AGR030
     primer_bind     4712..4736
                     /label=AGR029
     primer_bind     4857..4882
                     /label=045seq2
     primer_bind     4952..4975
                     /label=MBA276
     primer_bind     4956..4975
                     /label=MBA194
     primer_bind     complement(4974..5005)
                     /label=MBA210
     primer_bind     5021..5061
                     /label=47R
     primer_bind     5058..5083
                     /label=ptetF
     primer_bind     complement(5067..5093)
                     /label=MBA549
     primer_bind     complement(join(5070..5093,1..20))
                     /label=MBA147
     primer_bind     complement(5070..5093)
                     /label=MBA189
     primer_bind     complement(5070..5093)
                     /label=MBA161
     primer_bind     join(5091..5093,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagcgtcaa tttagtagta gaccacaagg
       61 aggttgctta atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct
      121 tcttgaaggt ttacgtcgtc tggaataccg cggatatgac tctgccggtc tggccgttgt
      181 tgatgcagaa ggtcatatga cccgcctgcg tcgcctcggt aaagtccaga tgctggcaca
      241 ggcagcggaa gaacatcctc tgcatggcgg cactggtatt gctcacactc gctgggcgac
      301 ccacggtgaa ccttcagaag tgaatgcgca tccgcatgtt tctgaacaca ttgtggtggt
      361 gcataacggc atcatcgaaa accatgaacc gctgcgtgaa gagctaaaag cgcgtggcta
      421 taccttcgtt tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa
      481 acaaggcggg actctgcgtg aggccgttct gcgtgctatc ccgcagctgc gtggtgcgta
      541 cggtacagtg atcatggact cccgtcaccc ggataccctg ctggcggcac gttctggtag
      601 tccgctggtg attggcctgg ggatgggcga aaactttatc gcttctgacc agctggcgct
      661 gttgccggtg acccgtcgct ttatcttcct tgaagagggc gatattgcgg aaatcactcg
      721 ccgttcggta aacatcttcg ataaaactgg cgcggaagta aaacgtcagg atatcgaatc
      781 caatctgcaa tatgacgcgg gcgataaagg catttaccgt cactacatgc agaaagagat
      841 ctacgaacag ccgaacgcga tcaaaaacac ccttaccgga cgcatcagcc acggtcaggt
      901 tgatttaagc gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca
      961 gatcctcgcc tgtggtactt cttataactc cggtatggtt tcccgctact ggtttgaatc
     1021 gctagcaggt attccgtgcg acgtcgaaat cgcctctgaa ttccgctatc gcaaatctgc
     1081 cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct ggcgaaaccg cggataccct
     1141 ggctggcctg cgtctgtcga aagagctggg ttaccttggt tcactggcaa tctgtaacgt
     1201 tccgggttct tctctggtgc gcgaatccga tctggcgcta atgaccaacg cgggtacaga
     1261 aatcggcgtg gcatccacta aagcattcac cactcagtta actgtgctgt tgatgctggt
     1321 ggcgaagctg tctcgcctga aaggtctgga tgcctccatt gaacatgaca tcgtgcatgg
     1381 tctgcaggcg ctgccgagcc gtattgagca gatgctgtct caggacaaac gcattgaagc
     1441 gctggcagaa gatttctctg acaaacatca cgcgctgttc ctgggccgtg gcgatcagta
     1501 cccaatcgcg ctggaaggcg cattgaagtt gaaagagatc tcttacattc acgctgaagc
     1561 ctacgctgct ggcgaactga aacacggtcc gctggcgcta attgatgccg atatgccggt
     1621 tattgttgtt gcaccgaaca acgaattgct ggaaaaactg aaatccaaca ttgaagaagt
     1681 tcgcgcgcgt ggcggtcagt tgtatgtctt cgccgatcag gatgcgggtt ttgtaagtag
     1741 cgataacatg cacatcatcg agatgccgca tgtggaagag gtgattgcac cgatcttcta
     1801 caccgttccg ctgcagctgc tggcttacca tgtcgcgctg atcaaaggca ccgacgttga
     1861 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa gaacgatacc taatagaaaa
     1921 taggaattga caccaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1981 cagattaatg ctgaagagga aattcgtcgt agtatcgatt ttctgaaaag ctacctgcaa
     2041 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     2101 gccggtaagc tgtgccagat ggcgattaat gagctgcgcc aggaaaccgg caacgaatca
     2161 ctgcaattta ttgccgtacg cctgccctat ggtgtccagg ccgacgaaca agattgccag
     2221 gatgccatcg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     2281 ctcgccagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     2341 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     2401 gtcgtggtgg gcaccgatca tgctgcagaa gctattaccg gattcttcac taaatatggt
     2461 gacggcggta cggacattaa tccgctgtat cgtctcaaca aacgtcaggg taaacagtta
     2521 ctggcggcat taggttgccc ggaacacctt tataagaaag cgccaacggc cgatcttgaa
     2581 gatgatcgcc cttctctgcc ggatgaagtg gcactcggcg tgacctatga caatatcgac
     2641 gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat
     2701 ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag
     2761 taaagcctca aagtaatcaa gccctggtaa ctgccagggc ttttttattt catctttgaa
     2821 tcaacatatt aaaattcatc gaatggcatc cttgctaaca gtgcttggat tctcaccaat
     2881 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2941 tactggatct atcaacagga gtccaagcga gctctcgaac cccagagtcc cagccaggac
     3001 agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg
     3061 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     3121 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     3181 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     3241 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     3301 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     3361 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     3421 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     3481 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     3541 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     3601 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     3661 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     3721 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3781 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3841 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3901 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3961 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     4021 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     4081 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaggatcc
     4141 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     4201 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     4261 cttcgggtgg gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga
     4321 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     4381 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     4441 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     4501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     4561 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     4621 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     4681 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4741 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     4801 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     4861 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     4921 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     4981 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     5041 tctcttcaaa tgtagcagag gctagtgtag atcgctacta gagcgcactg acc
//
