LOCUS       Exported                3952 bp ds-DNA     circular SYN 15-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          74..868
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          869..1123
                     /organism="Escherichia species"
                     /strain="strain"
                     /mol_type="genomic DNA"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..36)
                     /label=MBA645
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     2..23
                     /label=MBA759
     primer_bind     complement(7..36)
                     /label=MBA695
     RBS             36..73
                     /label=RBS (7504)
     primer_bind     73..107
                     /label=MBA704
     CDS             74..868
                     /codon_start=1
                     /transl_table=11
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /product="thymidylate synthetase"
                     /function="enzyme; 2'-Deoxyribonucleotide metabolism"
                     /EC_number="2.1.1.45"
                     /label=thyA
                     /note="GO_component: GO:0005737 - cytoplasm; GO_process: 
                     GO:0015949 - nucleobase, nucleoside and nucleotide 
                     interconversion"
                     /db_xref="ASAP:ABE-0009268"
                     /db_xref="UniProtKB/Swiss-Prot:P0A884"
                     /db_xref="EcoGene:EG11002"
                     /experiment="N-terminus verified by Edman degradation: PMID
                     6308660"
                     /protein_id="AAC75866.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            74..868
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /label=thyA
                     /db_xref="EcoGene:EG11002"
     primer_bind     complement(843..868)
                     /label=MBA703
     RBS             869..904
                     /label=RBS (4427)
     primer_bind     869..894
                     /label=AGR822
     CDS             905..1123
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="ODNLECBM_00895"
                     /product="translation initiation factor IF-1 [Escherichia 
                     coli Nissle 1917]"
                     /label=infA
                     /inference="ab initio prediction:same species:2.6"
                     /inference="similar to AA sequence:same species:AID81871.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYI
                     RILTGDKVTVELTPYDLSKGRIVFRSR"
     primer_bind     complement(1112..1123)
                     /label=AGR821
     primer_bind     1123..1156
                     /label=MBA696
     misc_feature    1124..1127
                     /label=BsaI Overlap
     primer_bind     1128..1157
                     /label=MBA286
     primer_bind     1128..1153
                     /label=AGR341
     primer_bind     complement(1155..1175)
                     /label=MBA489
     misc_feature    1182..1198
                     /label=113R
     primer_bind     1200..1220
                     /label=AGR526
     primer_bind     1200..1218
                     /label=MBA214
     primer_bind     complement(1201..1221)
                     /label=AGR366
     primer_bind     complement(1204..1225)
                     /label=MBA347
     primer_bind     complement(1207..1228)
                     /label=MBA015
     primer_bind     complement(1237..1262)
                     /label=AGR360
     primer_bind     complement(1257..1284)
                     /label=MBA177
     primer_bind     1281..1308
                     /label=AGR431
     primer_bind     complement(1322..1345)
                     /label=AGR301
     rep_origin      complement(1333..2283)
                     /direction=LEFT
                     /label=pSC101 ori
     primer_bind     1333..1360
                     /label=MBA206
     primer_bind     1991..2013
                     /label=MBA130
     primer_bind     complement(1991..2013)
                     /label=MBA129
     primer_bind     2212..2237
                     /label=JL025
     primer_bind     complement(2212..2237)
                     /label=AGR434
     RBS             2287..2299
                     /label=RBS for repA
     primer_bind     complement(2288..2323)
                     /label=MBA205
     promoter        complement(2304..2323)
                     /label=repA promoter
     primer_bind     2324..2354
                     /label=MBA228
     primer_bind     complement(2405..2431)
                     /label=MBA213
     primer_bind     2544..2571
                     /label=MBA1174
     primer_bind     complement(2661..2704)
                     /label=MBA1169
     primer_bind     complement(2685..2722)
                     /label=MBA439
     promoter        2685..2719
                     /label=BBa_J23119
     primer_bind     2685..2707
                     /label=MBA759
     primer_bind     2686..2719
                     /label=MBA646
     primer_bind     complement(2691..2719)
                     /label=MBA695
     misc_feature    2720..2794
                     /label=RiboJ
     primer_bind     2720..2779
                     /label=MBA1016
     misc_feature    2720..2722
                     /label=Overlap
     misc_feature    2720..2722
                     /label=SapI Landing Pad
     primer_bind     complement(2761..2794)
                     /label=MBA756
     primer_bind     2780..2836
                     /label=MBA1018
     primer_bind     complement(2783..2839)
                     /label=MBA1017
     primer_bind     2792..2836
                     /label=MBA394
     misc_feature    2792..2794
                     /label=Overlap
     primer_bind     complement(2795..2839)
                     /label=MBA395
     misc_feature    2795..2836
                     /label=RBS_21992
     CDS             2837..3592
                     /codon_start=1
                     /label=GFPmut3
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYA*VA"
     primer_bind     2837..2867
                     /label=AGR001
     primer_bind     2837..2867
                     /label=AGR041
     primer_bind     2837..2865
                     /label=AGR074
     misc_feature    2837..2839
                     /label=Overlap
     primer_bind     complement(2839..2867)
                     /label=AGR349
     primer_bind     complement(2909..2932)
                     /label=MBA133
     primer_bind     complement(2934..2954)
                     /label=MBA187
     primer_bind     complement(3059..3080)
                     /label=AGR011
     primer_bind     3358..3377
                     /label=AGR318
     primer_bind     complement(3542..3561)
                     /label=AGR319
     primer_bind     3557..3578
                     /label=Primer 1
     primer_bind     complement(3565..3612)
                     /label=MBA1166
     primer_bind     complement(3565..3592)
                     /label=AGR425
     primer_bind     complement(3566..3592)
                     /label=MBA117
     primer_bind     3593..3643
                     /label=MBA1168
     misc_feature    3593..3612
                     /label=Overlap
     primer_bind     3654..3672
                     /label=MBA493
     primer_bind     3776..3799
                     /label=MBA567
     misc_feature    3785..3807
                     /label=pTET For
     primer_bind     3794..3816
                     /label=MBA016
     primer_bind     complement(3794..3808)
                     /label=MBA147
     primer_bind     complement(3798..3818)
                     /label=MBA1175
     terminator      3808..3936
                     /label=dbI term
     primer_bind     complement(3811..3833)
                     /label=AGR157
     primer_bind     3883..3909
                     /label=MBA215
     primer_bind     complement(3918..3952)
                     /label=MAB553
     primer_bind     complement(3919..3949)
                     /label=AGR295
     primer_bind     complement(3919..3947)
                     /label=AGR420
     primer_bind     join(3950..3952,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcgac cgctatttta gcccatatca
       61 gaggtaacac ctaatgaaac agtatttaga actgatgcaa aaagtgctcg acgaaggcac
      121 acagaaaaac gaccgtaccg gaaccggaac gctttccatt tttggtcatc agatgcgttt
      181 taacctgcaa gatggattcc cgctggtgac aactaaacgt tgccacctgc gttccatcat
      241 ccatgaactg ctgtggtttc tgcagggcga cactaacatt gcttatctac acgaaaacaa
      301 tgtcaccatc tgggacgaat gggccgatga aaacggcgac ctcgggccag tgtatggtaa
      361 acagtggcgc gcctggccaa cgccagatgg tcgtcatatt gaccagatca ctacggtact
      421 gaaccagctg aaaaacgacc cggattcgcg ccgcattatt gtttcagcgt ggaacgtagg
      481 cgaactggat aaaatggcgc tggcaccgtg ccatgcattc ttccagttct atgtggcaga
      541 cggcaaactc tcttgccagc tttatcagcg ctcctgtgac gtcttcctcg gcctgccgtt
      601 caacattgcc agctacgcgt tattggtgca tatgatggcg cagcagtgcg atctggaagt
      661 gggtgatttt gtctggaccg gtggcgacac gcatctgtac agcaaccata tggatcaaac
      721 tcatctgcaa ttaagccgcg aaccgcgtcc gctgccgaag ttgattatca aacgtaaacc
      781 cgaatccatc ttcgactacc gtttcgaaga ctttgagatt gaaggctacg atccgcatcc
      841 gggcattaaa gcgccggtgg ctatctaagg ttcaaattac ggtagtgata ccccagagga
      901 ttagatggcc aaagaagaca atattgaaat gcaaggtacc gttcttgaaa cgttgcctaa
      961 taccatgttc cgcgtagagt tagaaaacgg tcacgtggtt actgcacaca tctccggtaa
     1021 aatgcgcaaa aactacatcc gcatcctgac gggcgacaaa gtgactgttg aactgacccc
     1081 gtacgacctg agcaaaggcc gcattgtctt ccgtagtcgc tgaagcccga gaaaagatta
     1141 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1201 cgagcggtat cagctcactc aaaggcggta atcccgggca gttttccctt tgatatctaa
     1261 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     1321 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     1381 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     1441 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     1501 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     1561 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     1621 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     1681 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     1741 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     1801 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     1861 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     1921 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     1981 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     2041 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     2101 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     2161 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     2221 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     2281 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     2341 atgataatta catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     2401 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     2461 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     2521 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     2581 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     2641 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat gttcttgaca gctagctcag
     2701 tcctaggtat aatgctagca gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga
     2761 ggacgaaaca gcctctacaa ataattttgt ttaaccatcc ctataacaaa aaatcagcgc
     2821 ggcaaagtag gtagagatga gtaaaggaga agaacttttc actggagttg tcccaattct
     2881 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     2941 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     3001 tccatggcca acacttgtca ctactttgac ttatggtgtt caatgctttt caagataccc
     3061 agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     3121 aagaactata tttttcaaag atgacgggaa ctataagaca cgtgctgaag tcaagtttga
     3181 aggtgataca cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     3241 cattcttgga cacaagttgg aatacaacta taactcacac aatgtataca tcatggcaga
     3301 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     3361 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     3421 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     3481 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     3541 actatacaaa aggcctgcag caaacgacga aaactacgct taagtagctt aagcggaaga
     3601 gttaagacca tttcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3661 tgacgggact cgagattacc attattgaag catttatcag ggttattgtc tcatgagcgg
     3721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcgaat
     3781 tcgtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     3841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     3901 ctggctcacc ttcgggtggg cctttctgcg tttatacaat tgtctagata cc
//
