LOCUS       Exported                3236 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          74..868
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          869..1123
                     /organism="Escherichia species"
                     /strain="strain"
                     /mol_type="genomic DNA"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..36)
                     /label=MBA645
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     2..23
                     /label=MBA759
     primer_bind     complement(2..20)
                     /label=MBA1171
     primer_bind     4..35
                     /label=AGR822
     primer_bind     complement(7..36)
                     /label=MBA695
     RBS             36..73
                     /label=RBS(7504)
     primer_bind     73..107
                     /label=MBA704
     CDS             74..868
                     /codon_start=1
                     /transl_table=11
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /product="thymidylate synthetase"
                     /function="enzyme; 2'-Deoxyribonucleotide metabolism"
                     /EC_number="2.1.1.45"
                     /label=thyA
                     /note="GO_component: GO:0005737 - cytoplasm; GO_process: 
                     GO:0015949 - nucleobase, nucleoside and nucleotide 
                     interconversion"
                     /db_xref="ASAP:ABE-0009268"
                     /db_xref="UniProtKB/Swiss-Prot:P0A884"
                     /db_xref="EcoGene:EG11002"
                     /experiment="N-terminus verified by Edman degradation: PMID
                     6308660"
                     /protein_id="AAC75866.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            74..868
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /label=thyA
                     /db_xref="EcoGene:EG11002"
     primer_bind     complement(843..868)
                     /label=MBA703
     primer_bind     865..899
                     /label=MBA822
     RBS             869..904
                     /label=RBS (4427)
     primer_bind     869..894
                     /label=AGR822
     CDS             905..1123
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="ODNLECBM_00895"
                     /product="translation initiation factor IF-1 [Escherichia 
                     coli Nissle 1917]"
                     /label=infA
                     /inference="ab initio prediction:same species:2.6"
                     /inference="similar to AA sequence:same species:AID81871.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYI
                     RILTGDKVTVELTPYDLSKGRIVFRSR"
     primer_bind     complement(1100..1128)
                     /label=MBA821
     primer_bind     1123..1154
                     /label=MBA697
     misc_feature    1124..1127
                     /label=BsaI Overlap
     primer_bind     1224..1249
                     /label=MBA1176
     primer_bind     complement(1254..1274)
                     /label=MBA277
     primer_bind     complement(1256..1274)
                     /label=MBA219
     primer_bind     complement(1261..1280)
                     /label=MBA118
     primer_bind     complement(1265..1286)
                     /label=MBA095
     primer_bind     complement(1266..1293)
                     /label=MBA191
     primer_bind     1266..1293
                     /label=MBA190
     primer_bind     complement(1328..1369)
                     /label=MBA1171
     primer_bind     complement(1329..1349)
                     /label=JL026
     primer_bind     complement(1331..1349)
                     /label=MBA075
     primer_bind     complement(1349..1369)
                     /label=MBA147
     primer_bind     complement(1350..1387)
                     /label=MBA439
     promoter        1350..1384
                     /label=BBa_J23119
     primer_bind     1350..1372
                     /label=MBA759
     primer_bind     1351..1384
                     /label=MBA646
     primer_bind     1353..1384
                     /label=AGR822
     primer_bind     complement(1356..1384)
                     /label=MBA695
     misc_feature    1385..1459
                     /label=RiboJ
     primer_bind     1385..1444
                     /label=MBA1016
     misc_feature    1385..1387
                     /label=SapI Landing Pad
     misc_feature    1385..1387
                     /label=Overlap
     primer_bind     complement(1388..1447)
                     /label=MBA1015
     primer_bind     complement(1426..1459)
                     /label=MBA756
     primer_bind     1445..1501
                     /label=MBA1018
     primer_bind     complement(1448..1504)
                     /label=MBA1017
     primer_bind     1457..1501
                     /label=MBA394
     misc_feature    1457..1459
                     /label=Overlap
     primer_bind     complement(1460..1504)
                     /label=MBA395
     misc_feature    1460..1501
                     /label=RBS_21992
     CDS             1502..2257
                     /codon_start=1
                     /label=GFPmut3
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYA*VA"
     primer_bind     1502..1532
                     /label=AGR001
     primer_bind     1502..1532
                     /label=AGR041
     primer_bind     1502..1530
                     /label=AGR074
     misc_feature    1502..1504
                     /label=Overlap
     primer_bind     complement(1504..1532)
                     /label=AGR349
     primer_bind     complement(1574..1597)
                     /label=MBA133
     primer_bind     complement(1599..1619)
                     /label=MBA187
     primer_bind     complement(1724..1745)
                     /label=AGR011
     primer_bind     2023..2042
                     /label=AGR318
     primer_bind     complement(2207..2226)
                     /label=AGR319
     primer_bind     2222..2243
                     /label=Primer 1
     primer_bind     complement(2230..2277)
                     /label=MBA1116
     primer_bind     complement(2230..2257)
                     /label=AGR425
     primer_bind     complement(2231..2257)
                     /label=MBA117
     primer_bind     2258..2303
                     /label=MBA1170
     misc_feature    2258..2277
                     /label=Overlap
     primer_bind     2278..2302
                     /label=MBA216
     primer_bind     2278..2302
                     /label=AGR199
     terminator      2302..2430
                     /label=dbl term
     primer_bind     complement(2305..2327)
                     /label=AGR157
     primer_bind     2377..2403
                     /label=MBA215
     primer_bind     complement(2413..2436)
                     /label=AGR222
     primer_bind     complement(2413..2431)
                     /label=AGR295
     primer_bind     complement(2436..2456)
                     /label=MBA1177
     rep_origin      complement(2437..3148)
                     /direction=LEFT
                     /label=p15a
     primer_bind     2437..2460
                     /label=MBA209
     primer_bind     2437..2460
                     /label=AGR221
     primer_bind     complement(2520..2541)
                     /label=AGR208
     primer_bind     complement(2541..2563)
                     /label=MBA800
     primer_bind     2549..2568
                     /label=MBA1184
     primer_bind     complement(2647..2664)
                     /label=MBA1185
     primer_bind     complement(2855..2881)
                     /label=MBA183
     primer_bind     2855..2881
                     /label=MBA182
     primer_bind     complement(2855..2879)
                     /label=AGR030
     primer_bind     2855..2879
                     /label=AGR029
     primer_bind     3000..3025
                     /label=045seq2
     primer_bind     3095..3118
                     /label=MBA276
     primer_bind     3099..3118
                     /label=MBA194
     primer_bind     complement(3117..3148)
                     /label=MBA210
     primer_bind     3164..3204
                     /label=47R
     primer_bind     3201..3226
                     /label=ptetF
     primer_bind     complement(3210..3236)
                     /label=MBA549
     primer_bind     complement(join(3213..3236,1..20))
                     /label=MBA147
     primer_bind     complement(3213..3236)
                     /label=MBA189
     primer_bind     complement(3213..3236)
                     /label=MBA161
     primer_bind     complement(3217..3236)
                     /label=MBA764
     primer_bind     join(3234..3236,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcgac cgctatttta gcccatatca
       61 gaggtaacac ctaatgaaac agtatttaga actgatgcaa aaagtgctcg acgaaggcac
      121 acagaaaaac gaccgtaccg gaaccggaac gctttccatt tttggtcatc agatgcgttt
      181 taacctgcaa gatggattcc cgctggtgac aactaaacgt tgccacctgc gttccatcat
      241 ccatgaactg ctgtggtttc tgcagggcga cactaacatt gcttatctac acgaaaacaa
      301 tgtcaccatc tgggacgaat gggccgatga aaacggcgac ctcgggccag tgtatggtaa
      361 acagtggcgc gcctggccaa cgccagatgg tcgtcatatt gaccagatca ctacggtact
      421 gaaccagctg aaaaacgacc cggattcgcg ccgcattatt gtttcagcgt ggaacgtagg
      481 cgaactggat aaaatggcgc tggcaccgtg ccatgcattc ttccagttct atgtggcaga
      541 cggcaaactc tcttgccagc tttatcagcg ctcctgtgac gtcttcctcg gcctgccgtt
      601 caacattgcc agctacgcgt tattggtgca tatgatggcg cagcagtgcg atctggaagt
      661 gggtgatttt gtctggaccg gtggcgacac gcatctgtac agcaaccata tggatcaaac
      721 tcatctgcaa ttaagccgcg aaccgcgtcc gctgccgaag ttgattatca aacgtaaacc
      781 cgaatccatc ttcgactacc gtttcgaaga ctttgagatt gaaggctacg atccgcatcc
      841 gggcattaaa gcgccggtgg ctatctaagg ttcaaattac ggtagtgata ccccagagga
      901 ttagatggcc aaagaagaca atattgaaat gcaaggtacc gttcttgaaa cgttgcctaa
      961 taccatgttc cgcgtagagt tagaaaacgg tcacgtggtt actgcacaca tctccggtaa
     1021 aatgcgcaaa aactacatcc gcatcctgac gggcgacaaa gtgactgttg aactgacccc
     1081 gtacgacctg agcaaaggcc gcattgtctt ccgtagtcgc tgaagcctca aagtaatcaa
     1141 gccctggtaa ctgccagggc ttttttattt catctttgaa tcaacatatt aaaattcatc
     1201 gaatggcatc cttgctaaca gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1261 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     1321 gtccaagcga gctctcgaac cccagagtct tgacagctag ctcagtccta ggtataatgc
     1381 tagcagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     1441 tacaaataat tttgtttaac catccctata acaaaaaatc agcgcggcaa agtaggtaga
     1501 gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1561 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1621 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1681 tgtcactact ttgacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg
     1741 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1801 caaagatgac gggaactata agacacgtgc tgaagtcaag tttgaaggtg atacacttgt
     1861 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1921 gttggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1981 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     2041 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2101 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2161 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaaaggcc
     2221 tgcagcaaac gacgaaaact acgcttaagt agcttaagcg gaagagttaa gaccattgga
     2281 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2341 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2401 caccttcggg tgggcctttc tgcgtttata cctagggata tattccgctt cctcgctcac
     2461 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
     2521 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
     2581 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
     2641 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc
     2701 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     2761 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     2821 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     2881 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     2941 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     3001 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     3061 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     3121 aacgatctca agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat
     3181 ttatctcttc aaatgtagca gaggctagtg tagatcgcta ctagagcgca ctgacc
//
