LOCUS       Exported                3826 bp ds-DNA     circular SYN 15-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          74..868
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          869..1123
                     /organism="Escherichia species"
                     /strain="strain"
                     /mol_type="genomic DNA"
     source          869..1123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..36)
                     /label=MBA645
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     2..23
                     /label=MBA759
     primer_bind     complement(7..36)
                     /label=MBA695
     RBS             36..73
                     /label=RBS(7504)
     primer_bind     73..107
                     /label=MBA704
     CDS             74..868
                     /codon_start=1
                     /transl_table=11
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /product="thymidylate synthetase"
                     /function="enzyme; 2'-Deoxyribonucleotide metabolism"
                     /EC_number="2.1.1.45"
                     /label=thyA
                     /note="GO_component: GO:0005737 - cytoplasm; GO_process: 
                     GO:0015949 - nucleobase, nucleoside and nucleotide 
                     interconversion"
                     /db_xref="ASAP:ABE-0009268"
                     /db_xref="UniProtKB/Swiss-Prot:P0A884"
                     /db_xref="EcoGene:EG11002"
                     /experiment="N-terminus verified by Edman degradation: PMID
                     6308660"
                     /protein_id="AAC75866.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLV
                     TTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTP
                     DGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQL
                     YQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSR
                     EPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            74..868
                     /gene="thyA"
                     /gene_synonym="ECK2823;JW2795"
                     /locus_tag="b2827"
                     /label=thyA
                     /db_xref="EcoGene:EG11002"
     primer_bind     complement(843..868)
                     /label=MBA703
     primer_bind     865..899
                     /label=MBA822
     RBS             869..904
                     /label=RBS (4427)
     primer_bind     869..894
                     /label=AGR822
     CDS             905..1123
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="ODNLECBM_00895"
                     /product="translation initiation factor IF-1 [Escherichia 
                     coli Nissle 1917]"
                     /label=infA
                     /inference="ab initio prediction:same species:2.6"
                     /inference="similar to AA sequence:same species:AID81871.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNYI
                     RILTGDKVTVELTPYDLSKGRIVFRSR"
     primer_bind     complement(1100..1127)
                     /label=MBA821
     primer_bind     1123..1158
                     /label=MBA698
     misc_feature    1124..1127
                     /label=BsaI Overlap
     misc_feature    1160..1193
                     /label=his [min] terminator
     primer_bind     complement(1233..1253)
                     /label=MBA489
     primer_bind     1278..1296
                     /label=MBA214
     primer_bind     complement(1282..1303)
                     /label=MBA347
     primer_bind     complement(1285..1306)
                     /label=MBA015
     primer_bind     complement(1291..1312)
                     /label=MBA141
     primer_bind     complement(1291..1312)
                     /label=MBA225
     primer_bind     1374..1394
                     /label=MBA195
     rep_origin      complement(1392..2074)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     complement(1696..1722)
                     /label=MBA183
     primer_bind     1696..1722
                     /label=MBA182
     primer_bind     2043..2068
                     /label=MBA1130
     primer_bind     complement(2049..2074)
                     /label=MBA196
     primer_bind     complement(2132..2188)
                     /label=MBA1173
     primer_bind     complement(2132..2168)
                     /label=MBA265
     primer_bind     complement(2169..2206)
                     /label=MBA439
     promoter        2169..2203
                     /label=BBa_J23119
     primer_bind     2169..2191
                     /label=MBA759
     primer_bind     2170..2203
                     /label=MBA646
     primer_bind     complement(2175..2203)
                     /label=MBA695
     misc_feature    2204..2278
                     /label=RiboJ
     primer_bind     2204..2263
                     /label=MBA1016
     misc_feature    2204..2206
                     /label=Overlap
     misc_feature    2204..2206
                     /label=SapI Landing Pad
     primer_bind     complement(2245..2278)
                     /label=MBA756
     primer_bind     2264..2320
                     /label=MBA1018
     primer_bind     complement(2267..2323)
                     /label=MBA1017
     primer_bind     2276..2320
                     /label=MBA394
     misc_feature    2276..2278
                     /label=Overlap
     primer_bind     complement(2279..2323)
                     /label=MBA395
     misc_feature    2279..2320
                     /label=RBS_21992
     CDS             2321..3076
                     /codon_start=1
                     /label=GFPmut3
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYA*VA"
     primer_bind     2321..2351
                     /label=AGR041
     primer_bind     2321..2351
                     /label=AGR001
     primer_bind     2321..2349
                     /label=AGR074
     misc_feature    2321..2323
                     /label=Overlap
     primer_bind     complement(2323..2351)
                     /label=AGR349
     primer_bind     complement(2393..2416)
                     /label=MBA133
     primer_bind     complement(2418..2438)
                     /label=MBA187
     primer_bind     complement(2543..2564)
                     /label=AGR011
     primer_bind     2842..2861
                     /label=AGR318
     primer_bind     complement(3026..3045)
                     /label=AGR319
     primer_bind     complement(3049..3096)
                     /label=MBA1166
     primer_bind     complement(3049..3076)
                     /label=AGR425
     primer_bind     complement(3050..3076)
                     /label=MBA117
     primer_bind     3077..3117
                     /label=MBA1172
     misc_feature    3077..3096
                     /label=Overlap
     primer_bind     3097..3108
                     /label=MBA188
     primer_bind     complement(3107..3157)
                     /label=MBA267
     primer_bind     3138..3157
                     /label=MBA268
     primer_bind     3238..3258
                     /label=MBA564
     primer_bind     complement(3240..3261)
                     /label=MBA275
     terminator      3263..3319
                     /label=TJC Eck1200034435 terminator
     CDS             complement(3323..3342)
                     /codon_start=1
                     /label=LuxR
                     /translation="PYFKN"
     misc_feature    3340..3342
                     /label=Overlap
     misc_feature    3343..3399
                     /label=For BreR Eventual Insertion?
     primer_bind     complement(3348..3375)
                     /label=MBA269
     primer_bind     3356..3395
                     /label=MBA270
     primer_bind     complement(3364..3375)
                     /label=MBA223
     primer_bind     3539..3564
                     /label=MBA038
     primer_bind     complement(3596..3615)
                     /label=MBA565
     primer_bind     3701..3719
                     /label=MBA566
     primer_bind     join(3824..3826,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctcgac cgctatttta gcccatatca
       61 gaggtaacac ctaatgaaac agtatttaga actgatgcaa aaagtgctcg acgaaggcac
      121 acagaaaaac gaccgtaccg gaaccggaac gctttccatt tttggtcatc agatgcgttt
      181 taacctgcaa gatggattcc cgctggtgac aactaaacgt tgccacctgc gttccatcat
      241 ccatgaactg ctgtggtttc tgcagggcga cactaacatt gcttatctac acgaaaacaa
      301 tgtcaccatc tgggacgaat gggccgatga aaacggcgac ctcgggccag tgtatggtaa
      361 acagtggcgc gcctggccaa cgccagatgg tcgtcatatt gaccagatca ctacggtact
      421 gaaccagctg aaaaacgacc cggattcgcg ccgcattatt gtttcagcgt ggaacgtagg
      481 cgaactggat aaaatggcgc tggcaccgtg ccatgcattc ttccagttct atgtggcaga
      541 cggcaaactc tcttgccagc tttatcagcg ctcctgtgac gtcttcctcg gcctgccgtt
      601 caacattgcc agctacgcgt tattggtgca tatgatggcg cagcagtgcg atctggaagt
      661 gggtgatttt gtctggaccg gtggcgacac gcatctgtac agcaaccata tggatcaaac
      721 tcatctgcaa ttaagccgcg aaccgcgtcc gctgccgaag ttgattatca aacgtaaacc
      781 cgaatccatc ttcgactacc gtttcgaaga ctttgagatt gaaggctacg atccgcatcc
      841 gggcattaaa gcgccggtgg ctatctaagg ttcaaattac ggtagtgata ccccagagga
      901 ttagatggcc aaagaagaca atattgaaat gcaaggtacc gttcttgaaa cgttgcctaa
      961 taccatgttc cgcgtagagt tagaaaacgg tcacgtggtt actgcacaca tctccggtaa
     1021 aatgcgcaaa aactacatcc gcatcctgac gggcgacaaa gtgactgttg aactgacccc
     1081 gtacgacctg agcaaaggcc gcattgtctt ccgtagtcgc tgaagccgag caagacaatg
     1141 gtaagtagta atagctcctg cccccggaag atcattccgg gggctttttt attcccactg
     1201 tgaactggat ccttactcga gtctagactg caggcttcct cgctcactga ctcgctgcgc
     1261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1381 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
     1441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1921 gccaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2161 tctgacagtt gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg
     2221 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaacc
     2281 atccctataa caaaaaatca gcgcggcaaa gtaggtagag atgagtaaag gagaagaact
     2341 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     2401 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     2461 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tgacttatgg
     2521 tgttcaatgc ttttcaagat acccagatca tatgaaacgg catgactttt tcaagagtgc
     2581 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactataa
     2641 gacacgtgct gaagtcaagt ttgaaggtga tacacttgtt aatagaatcg agttaaaagg
     2701 tattgatttt aaagaagatg gaaacattct tggacacaag ttggaataca actataactc
     2761 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     2821 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     2881 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     2941 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     3001 tgggattaca catggcatgg atgaactata caaaaggcct gcagcaaacg acgaaaacta
     3061 cgcttaagta gcttaagcgg aagagttaag accattcctc ttacgtgccg atcaacgtct
     3121 cattttcgcc agatatcggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcatcaccc
     3241 taggtgtaaa acgacggcca gactcggtac caaattccag aaaagagacg ctgaaaagcg
     3301 tcttttttcg ttttggtcct tattaatttt taaagtatgg gcttaaccag attagatccc
     3361 gtagatgtcc atccccgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     3421 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     3481 agcggtcctc tccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     3541 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     3601 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc gggcttatcg actgcacggt
     3661 gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt
     3721 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
     3781 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgacc
//
