LOCUS       Exported                4748 bp ds-DNA     circular SYN 29-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          71..1900
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     2..23
                     /label=MBA759
     primer_bind     complement(2..20)
                     /label=MBA1171
     primer_bind     complement(7..36)
                     /label=MBA1155
     primer_bind     complement(7..35)
                     /label=MBA695
     primer_bind     complement(11..63)
                     /label=MBA816
     primer_bind     complement(11..36)
                     /label=JL064
     RBS             36..70
                     /label=RBS (8455)
     primer_bind     70..91
                     /label=MBA702
     gene            71..1900
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /label=glmS
                     /db_xref="EcoGene:EG10382"
     CDS             71..1900
                     /codon_start=1
                     /transl_table=11
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /product="L-glutamine:D-fructose-6-phosphate 
                     aminotransferase"
                     /function="enzyme; Central intermediary metabolism: Amino 
                     sugars"
                     /EC_number="2.6.1.16"
                     /label=glmS
                     /note="GO_component: GO:0009274 - peptidoglycan-based cell 
                     wall; GO_component: GO:0005737 - cytoplasm; GO_function: 
                     GO:0042280 - cell surface antigen activity, 
                     host-interacting; GO_process: GO:0009243 - O antigen 
                     biosynthetic process; GO_process: GO:0009252 - 
                     peptidoglycan biosynthetic process; GO_process: GO:0046349 
                     - amino sugar biosynthetic process"
                     /db_xref="ASAP:ABE-0012198"
                     /db_xref="UniProtKB/Swiss-Prot:P17169"
                     /db_xref="EcoGene:EG10382"
                     /protein_id="AAC76752.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMTR
                     LRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGIIEN
                     HEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTVIMD
                     SRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRRSVNI
                     FDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQVDLSE
                     LGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYRKSAVRR
                     NSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMTNAGTEIG
                     VASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIEAL
                     AEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPV
                     IVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIF
                     YTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     primer_bind     complement(502..514)
                     /label=AGR222
     primer_bind     complement(502..514)
                     /label=AGR295
     primer_bind     1742..1766
                     /label=MBA1116
     primer_bind     complement(1876..1900)
                     /label=MBA701
     RBS             1901..1935
                     /label=nadE RBS (5392)
     primer_bind     complement(1901..1931)
                     /label=MBA1155
     primer_bind     1912..1964
                     /label=MBA1156
     CDS             1936..2763
                     /codon_start=1
                     /label=nadE
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLVL
                     GISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQPD
                     RVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGTDHA
                     AEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDRPSLP
                     DEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     primer_bind     complement(2735..2763)
                     /label=MBA1157
     primer_bind     2763..2794
                     /label=MBA697
     misc_feature    2764..2767
                     /label=BsaI Overlap
     primer_bind     2864..2889
                     /label=MBA1176
     primer_bind     complement(2894..2914)
                     /label=MBA277
     primer_bind     complement(2896..2914)
                     /label=MBA219
     primer_bind     complement(2901..2920)
                     /label=MBA118
     primer_bind     complement(2905..2926)
                     /label=MBA095
     primer_bind     complement(2906..2933)
                     /label=MBA191
     primer_bind     2906..2933
                     /label=MBA190
     primer_bind     complement(2968..3009)
                     /label=MBA1171
     primer_bind     complement(2969..2989)
                     /label=JL026
     primer_bind     complement(2971..2989)
                     /label=MBA075
     primer_bind     complement(2989..3009)
                     /label=MBA147
     promoter        2990..3024
                     /label=BBa_J23119
     primer_bind     2990..3012
                     /label=MBA759
     primer_bind     2991..3024
                     /label=MBA646
     primer_bind     complement(2996..3025)
                     /label=MBA695
     primer_bind     complement(2996..3024)
                     /label=MBA1155
     primer_bind     complement(3000..3052)
                     /label=MBA816
     primer_bind     complement(3000..3024)
                     /label=JL064
     RBS             3025..3058
                     /label=RBS (22821)
     primer_bind     3033..3077
                     /label=MBA817
     CDS             3059..3769
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3183..3201)
                     /label=MBA388
     primer_bind     complement(3746..3789)
                     /label=MBA1167
     primer_bind     complement(3746..3770)
                     /label=MBA818
     primer_bind     complement(3746..3770)
                     /label=AGR874
     primer_bind     complement(3746..3769)
                     /label=AGR311
     primer_bind     3770..3815
                     /label=MBA1170
     misc_feature    3770..3789
                     /label=Overlap
     primer_bind     3790..3814
                     /label=MBA216
     primer_bind     3790..3814
                     /label=AGR199
     terminator      3814..3942
                     /label=dbl term
     primer_bind     complement(3817..3839)
                     /label=AGR157
     primer_bind     3889..3915
                     /label=MBA215
     primer_bind     complement(3925..3948)
                     /label=AGR222
     primer_bind     complement(3925..3943)
                     /label=AGR295
     primer_bind     complement(3948..3968)
                     /label=MBA1177
     rep_origin      complement(3949..4660)
                     /direction=LEFT
                     /label=p15a
     primer_bind     3949..3972
                     /label=MBA209
     primer_bind     3949..3972
                     /label=AGR221
     primer_bind     complement(4032..4053)
                     /label=AGR208
     primer_bind     4061..4080
                     /label=MBA1184
     primer_bind     complement(4159..4176)
                     /label=MBA1185
     primer_bind     complement(4367..4393)
                     /label=MBA183
     primer_bind     4367..4393
                     /label=MBA182
     primer_bind     complement(4367..4391)
                     /label=AGR030
     primer_bind     4367..4391
                     /label=AGR029
     primer_bind     4512..4537
                     /label=045seq2
     primer_bind     4607..4630
                     /label=MBA276
     primer_bind     4611..4630
                     /label=MBA194
     primer_bind     complement(4629..4660)
                     /label=MBA210
     primer_bind     4676..4716
                     /label=47R
     primer_bind     4713..4738
                     /label=ptetF
     primer_bind     complement(4722..4748)
                     /label=MBA549
     primer_bind     complement(join(4725..4748,1..20))
                     /label=MBA147
     primer_bind     complement(4725..4748)
                     /label=MBA189
     primer_bind     complement(4725..4748)
                     /label=MBA161
     primer_bind     join(4746..4748,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagcgtcaa tttagtagta gaccacaagg
       61 aggttgctta atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct
      121 tcttgaaggt ttacgtcgtc tggaataccg cggatatgac tctgccggtc tggccgttgt
      181 tgatgcagaa ggtcatatga cccgcctgcg tcgcctcggt aaagtccaga tgctggcaca
      241 ggcagcggaa gaacatcctc tgcatggcgg cactggtatt gctcacactc gctgggcgac
      301 ccacggtgaa ccttcagaag tgaatgcgca tccgcatgtt tctgaacaca ttgtggtggt
      361 gcataacggc atcatcgaaa accatgaacc gctgcgtgaa gagctaaaag cgcgtggcta
      421 taccttcgtt tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa
      481 acaaggcggg actctgcgtg aggccgttct gcgtgctatc ccgcagctgc gtggtgcgta
      541 cggtacagtg atcatggact cccgtcaccc ggataccctg ctggcggcac gttctggtag
      601 tccgctggtg attggcctgg ggatgggcga aaactttatc gcttctgacc agctggcgct
      661 gttgccggtg acccgtcgct ttatcttcct tgaagagggc gatattgcgg aaatcactcg
      721 ccgttcggta aacatcttcg ataaaactgg cgcggaagta aaacgtcagg atatcgaatc
      781 caatctgcaa tatgacgcgg gcgataaagg catttaccgt cactacatgc agaaagagat
      841 ctacgaacag ccgaacgcga tcaaaaacac ccttaccgga cgcatcagcc acggtcaggt
      901 tgatttaagc gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca
      961 gatcctcgcc tgtggtactt cttataactc cggtatggtt tcccgctact ggtttgaatc
     1021 gctagcaggt attccgtgcg acgtcgaaat cgcctctgaa ttccgctatc gcaaatctgc
     1081 cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct ggcgaaaccg cggataccct
     1141 ggctggcctg cgtctgtcga aagagctggg ttaccttggt tcactggcaa tctgtaacgt
     1201 tccgggttct tctctggtgc gcgaatccga tctggcgcta atgaccaacg cgggtacaga
     1261 aatcggcgtg gcatccacta aagcattcac cactcagtta actgtgctgt tgatgctggt
     1321 ggcgaagctg tctcgcctga aaggtctgga tgcctccatt gaacatgaca tcgtgcatgg
     1381 tctgcaggcg ctgccgagcc gtattgagca gatgctgtct caggacaaac gcattgaagc
     1441 gctggcagaa gatttctctg acaaacatca cgcgctgttc ctgggccgtg gcgatcagta
     1501 cccaatcgcg ctggaaggcg cattgaagtt gaaagagatc tcttacattc acgctgaagc
     1561 ctacgctgct ggcgaactga aacacggtcc gctggcgcta attgatgccg atatgccggt
     1621 tattgttgtt gcaccgaaca acgaattgct ggaaaaactg aaatccaaca ttgaagaagt
     1681 tcgcgcgcgt ggcggtcagt tgtatgtctt cgccgatcag gatgcgggtt ttgtaagtag
     1741 cgataacatg cacatcatcg agatgccgca tgtggaagag gtgattgcac cgatcttcta
     1801 caccgttccg ctgcagctgc tggcttacca tgtcgcgctg atcaaaggca ccgacgttga
     1861 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa gaacgatacc taatagaaaa
     1921 taggaattga caccaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1981 cagattaatg ctgaagagga aattcgtcgt agtatcgatt ttctgaaaag ctacctgcaa
     2041 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     2101 gccggtaagc tgtgccagat ggcgattaat gagctgcgcc aggaaaccgg caacgaatca
     2161 ctgcaattta ttgccgtacg cctgccctat ggtgtccagg ccgacgaaca agattgccag
     2221 gatgccatcg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     2281 ctcgccagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     2341 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     2401 gtcgtggtgg gcaccgatca tgctgcagaa gctattaccg gattcttcac taaatatggt
     2461 gacggcggta cggacattaa tccgctgtat cgtctcaaca aacgtcaggg taaacagtta
     2521 ctggcggcat taggttgccc ggaacacctt tataagaaag cgccaacggc cgatcttgaa
     2581 gatgatcgcc cttctctgcc ggatgaagtg gcactcggcg tgacctatga caatatcgac
     2641 gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat
     2701 ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag
     2761 taaagcctca aagtaatcaa gccctggtaa ctgccagggc ttttttattt catctttgaa
     2821 tcaacatatt aaaattcatc gaatggcatc cttgctaaca gtgcttggat tctcaccaat
     2881 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2941 tactggatct atcaacagga gtccaagcga gctctcgaac cccagagtct tgacagctag
     3001 ctcagtccta ggtataatgc tagcttctta attacgtagg accataagga ggtttcctat
     3061 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3121 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3181 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3241 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     3301 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3361 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     3421 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     3481 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     3541 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3601 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3661 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3721 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggaagagtt
     3781 aagaccattg gatccaaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc
     3841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     3901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tacctaggga tatattccgc
     3961 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     4021 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     4081 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     4141 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4201 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     4261 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     4321 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     4381 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     4441 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     4501 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     4561 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     4621 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     4681 tttcagtgca atttatctct tcaaatgtag cagaggctag tgtagatcgc tactagagcg
     4741 cactgacc
//
