LOCUS       Exported                5338 bp ds-DNA     circular SYN 15-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Matt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          71..1900
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:511145"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1901..2763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..35
                     /label=BBa_J23108
     primer_bind     2..23
                     /label=MBA759
     primer_bind     complement(7..36)
                     /label=MBA1155
     primer_bind     complement(7..35)
                     /label=MBA695
     primer_bind     complement(11..63)
                     /label=MBA816
     primer_bind     complement(11..36)
                     /label=JL064
     RBS             36..70
                     /label=RBS (8455)
     primer_bind     70..91
                     /label=MBA702
     gene            71..1900
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /label=glmS
                     /db_xref="EcoGene:EG10382"
     CDS             71..1900
                     /codon_start=1
                     /transl_table=11
                     /gene="glmS"
                     /gene_synonym="ECK3722;JW3707"
                     /locus_tag="b3729"
                     /product="L-glutamine:D-fructose-6-phosphate 
                     aminotransferase"
                     /function="enzyme; Central intermediary metabolism: Amino 
                     sugars"
                     /EC_number="2.6.1.16"
                     /label=glmS
                     /note="GO_component: GO:0009274 - peptidoglycan-based cell 
                     wall; GO_component: GO:0005737 - cytoplasm; GO_function: 
                     GO:0042280 - cell surface antigen activity, 
                     host-interacting; GO_process: GO:0009243 - O antigen 
                     biosynthetic process; GO_process: GO:0009252 - 
                     peptidoglycan biosynthetic process; GO_process: GO:0046349 
                     - amino sugar biosynthetic process"
                     /db_xref="ASAP:ABE-0012198"
                     /db_xref="UniProtKB/Swiss-Prot:P17169"
                     /db_xref="EcoGene:EG10382"
                     /protein_id="AAC76752.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMTR
                     LRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGIIEN
                     HEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTVIMD
                     SRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRRSVNI
                     FDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQVDLSE
                     LGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYRKSAVRR
                     NSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMTNAGTEIG
                     VASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIEAL
                     AEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPLALIDADMPV
                     IVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIF
                     YTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     primer_bind     1742..1766
                     /label=MBA1116
     primer_bind     complement(1876..1900)
                     /label=MBA701
     RBS             1901..1935
                     /label=nadE RBS (5392)
     primer_bind     1912..1964
                     /label=MBA1156
     CDS             1936..2763
                     /codon_start=1
                     /label=nadE
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLVL
                     GISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQPD
                     RVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGTDHA
                     AEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDRPSLP
                     DEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     primer_bind     complement(2735..2763)
                     /label=MBA1157
     primer_bind     2763..2798
                     /label=MBA698
     misc_feature    2764..2767
                     /label=BsaI Overlap
     misc_feature    2800..2833
                     /label=his [min] terminator
     primer_bind     complement(2873..2893)
                     /label=MBA489
     primer_bind     2918..2936
                     /label=MBA214
     primer_bind     complement(2922..2943)
                     /label=MBA347
     primer_bind     complement(2925..2946)
                     /label=MBA015
     primer_bind     complement(2931..2952)
                     /label=MBA141
     primer_bind     complement(2931..2952)
                     /label=MBA225
     primer_bind     3014..3034
                     /label=MBA195
     rep_origin      complement(3032..3714)
                     /direction=LEFT
                     /label=ColE1
     primer_bind     complement(3336..3362)
                     /label=MBA183
     primer_bind     3336..3362
                     /label=MBA182
     primer_bind     3683..3708
                     /label=MBA1130
     primer_bind     complement(3689..3714)
                     /label=MBA196
     primer_bind     complement(3772..3828)
                     /label=MBA1173
     primer_bind     complement(3772..3808)
                     /label=MBA265
     promoter        3809..3843
                     /label=BBa_J23119
     primer_bind     3809..3831
                     /label=MBA759
     primer_bind     3810..3843
                     /label=MBA646
     primer_bind     complement(3815..3844)
                     /label=MBA695
     primer_bind     complement(3815..3843)
                     /label=MBA1155
     primer_bind     complement(3819..3871)
                     /label=MBA816
     primer_bind     complement(3819..3843)
                     /label=JL064
     RBS             3844..3877
                     /label=RBS (22821)
     primer_bind     3852..3896
                     /label=MBA817
     CDS             3878..4588
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4002..4020)
                     /label=MBA388
     primer_bind     complement(4565..4608)
                     /label=MBA1167
     primer_bind     complement(4565..4589)
                     /label=AGR874
     primer_bind     complement(4565..4589)
                     /label=MBA818
     primer_bind     complement(4565..4588)
                     /label=AGR311
     primer_bind     4589..4629
                     /label=MBA1172
     misc_feature    4589..4608
                     /label=Overlap
     primer_bind     4609..4629
                     /label=MBA1009
     primer_bind     4609..4620
                     /label=MBA188
     primer_bind     complement(4619..4669)
                     /label=MBA267
     primer_bind     complement(4628..4650)
                     /label=MBA1131
     primer_bind     complement(4631..4650)
                     /label=MBA077
     primer_bind     4650..4669
                     /label=MBA268
     primer_bind     4750..4770
                     /label=MBA564
     primer_bind     complement(4752..4773)
                     /label=MBA275
     terminator      4775..4831
                     /label=TJC Eck1200034435 terminator
     CDS             complement(4835..4854)
                     /codon_start=1
                     /label=LuxR
                     /translation="PYFKN"
     misc_feature    4852..4854
                     /label=Overlap
     misc_feature    4855..4911
                     /label=For BreR Eventual Insertion?
     primer_bind     complement(4860..4887)
                     /label=MBA269
     primer_bind     4868..4907
                     /label=MBA270
     primer_bind     complement(4876..4887)
                     /label=MBA223
     primer_bind     5051..5076
                     /label=MBA038
     primer_bind     complement(5108..5127)
                     /label=MBA565
     primer_bind     5213..5231
                     /label=MBA566
     primer_bind     join(5336..5338,1..35)
                     /label=MBA646
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagcgtcaa tttagtagta gaccacaagg
       61 aggttgctta atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct
      121 tcttgaaggt ttacgtcgtc tggaataccg cggatatgac tctgccggtc tggccgttgt
      181 tgatgcagaa ggtcatatga cccgcctgcg tcgcctcggt aaagtccaga tgctggcaca
      241 ggcagcggaa gaacatcctc tgcatggcgg cactggtatt gctcacactc gctgggcgac
      301 ccacggtgaa ccttcagaag tgaatgcgca tccgcatgtt tctgaacaca ttgtggtggt
      361 gcataacggc atcatcgaaa accatgaacc gctgcgtgaa gagctaaaag cgcgtggcta
      421 taccttcgtt tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa
      481 acaaggcggg actctgcgtg aggccgttct gcgtgctatc ccgcagctgc gtggtgcgta
      541 cggtacagtg atcatggact cccgtcaccc ggataccctg ctggcggcac gttctggtag
      601 tccgctggtg attggcctgg ggatgggcga aaactttatc gcttctgacc agctggcgct
      661 gttgccggtg acccgtcgct ttatcttcct tgaagagggc gatattgcgg aaatcactcg
      721 ccgttcggta aacatcttcg ataaaactgg cgcggaagta aaacgtcagg atatcgaatc
      781 caatctgcaa tatgacgcgg gcgataaagg catttaccgt cactacatgc agaaagagat
      841 ctacgaacag ccgaacgcga tcaaaaacac ccttaccgga cgcatcagcc acggtcaggt
      901 tgatttaagc gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca
      961 gatcctcgcc tgtggtactt cttataactc cggtatggtt tcccgctact ggtttgaatc
     1021 gctagcaggt attccgtgcg acgtcgaaat cgcctctgaa ttccgctatc gcaaatctgc
     1081 cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct ggcgaaaccg cggataccct
     1141 ggctggcctg cgtctgtcga aagagctggg ttaccttggt tcactggcaa tctgtaacgt
     1201 tccgggttct tctctggtgc gcgaatccga tctggcgcta atgaccaacg cgggtacaga
     1261 aatcggcgtg gcatccacta aagcattcac cactcagtta actgtgctgt tgatgctggt
     1321 ggcgaagctg tctcgcctga aaggtctgga tgcctccatt gaacatgaca tcgtgcatgg
     1381 tctgcaggcg ctgccgagcc gtattgagca gatgctgtct caggacaaac gcattgaagc
     1441 gctggcagaa gatttctctg acaaacatca cgcgctgttc ctgggccgtg gcgatcagta
     1501 cccaatcgcg ctggaaggcg cattgaagtt gaaagagatc tcttacattc acgctgaagc
     1561 ctacgctgct ggcgaactga aacacggtcc gctggcgcta attgatgccg atatgccggt
     1621 tattgttgtt gcaccgaaca acgaattgct ggaaaaactg aaatccaaca ttgaagaagt
     1681 tcgcgcgcgt ggcggtcagt tgtatgtctt cgccgatcag gatgcgggtt ttgtaagtag
     1741 cgataacatg cacatcatcg agatgccgca tgtggaagag gtgattgcac cgatcttcta
     1801 caccgttccg ctgcagctgc tggcttacca tgtcgcgctg atcaaaggca ccgacgttga
     1861 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa gaacgatacc taatagaaaa
     1921 taggaattga caccaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1981 cagattaatg ctgaagagga aattcgtcgt agtatcgatt ttctgaaaag ctacctgcaa
     2041 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     2101 gccggtaagc tgtgccagat ggcgattaat gagctgcgcc aggaaaccgg caacgaatca
     2161 ctgcaattta ttgccgtacg cctgccctat ggtgtccagg ccgacgaaca agattgccag
     2221 gatgccatcg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     2281 ctcgccagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     2341 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     2401 gtcgtggtgg gcaccgatca tgctgcagaa gctattaccg gattcttcac taaatatggt
     2461 gacggcggta cggacattaa tccgctgtat cgtctcaaca aacgtcaggg taaacagtta
     2521 ctggcggcat taggttgccc ggaacacctt tataagaaag cgccaacggc cgatcttgaa
     2581 gatgatcgcc cttctctgcc ggatgaagtg gcactcggcg tgacctatga caatatcgac
     2641 gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga gaactggtat
     2701 ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag
     2761 taaagccgag caagacaatg gtaagtagta atagctcctg cccccggaag atcattccgg
     2821 gggctttttt attcccactg tgaactggat ccttactcga gtctagactg caggcttcct
     2881 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2941 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3001 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
     3061 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3121 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3181 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3241 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3301 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3361 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3421 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3481 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3541 gagttggtag ctcttgatcc gccaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3601 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3661 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3721 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3781 gtatatatga gtaaacttgg tctgacagtt gacagctagc tcagtcctag gtataatgct
     3841 agcttcttaa ttacgtagga ccataaggag gtttcctatg gtgagcaagg gcgaggagga
     3901 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3961 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4021 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     4141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4201 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4561 cggcggcatg gacgagctgt acaagtaagc ggaagagtta agaccattcc tcttacgtgc
     4621 cgatcaacgt ctcattttcg ccagatatcg ggttccgcgc acatttcccc gaaaagtgcc
     4681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4741 gaggcatcac cctaggtgta aaacgacggc cagactcggt accaaattcc agaaaagaga
     4801 cgctgaaaag cgtctttttt cgttttggtc cttattaatt tttaaagtat gggcttaacc
     4861 agattagatc ccgtagatgt ccatccccgc cgaaacgttt ggtggcggga ccagtgacga
     4921 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     4981 cgctccagcg aaagcggtcc tctccgaaaa tgacccagag cgctgccggc acctgtccta
     5041 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     5101 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat ccgggcttat
     5161 cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc
     5221 tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat
     5281 aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgacc
//
