LOCUS       pAAV-EF1a-PGD2-1        7045 bp DNA     circular SYN 17-JUN-2023
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|897177600|
COMMENT     VNTDBDATE|1024444800|
COMMENT     VNTNAME|pAAV-EF1a-PGD2-1.1|
FEATURES             Location/Qualifiers
     misc_feature    1303..3231
                     /mol_type="other DNA"
                     /label=nonstandard type: source
                     /organism="pDisplay-BirA-ER"
     promoter        101..1278
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     primer          1180..1199
                     /label=EF-seq-f
                     /note="sequence: TTCTCCTTGGAATTTGCCCT"
     primer          1273..1322
                     /label=EFa-igK-F
                     /note="sequence: 
                     TCGTGAGGTACCGGATCCTCTAGAGTCGACATGGAGACAGACACACTCCT"
     sig_peptide     1303..1365
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
     protein_bind    1366..1390
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer          1369..1398
                     /label=F-pdisplay-Age1-DP1
                     /note="sequence: AGTTTGTACAAAAAAGTTGGCACCaccggt"
     primer          complement(1370..1392)
                     /label=R-pdisplayJM
                     /note="sequence: GGTGCCAACTTTTTTGTACAAAC"
     misc_feature    1399..3228
                     /label=nonstandard type: gene
                     /label=dp1-bat
     misc_feature    1846..1851
                     /label=mut
     misc_feature    2029..2031
                     /label=L5.50
     misc_feature    2050..2097
                     /label=screened
     misc_feature    2058..2073
                     /label=nonstandard type: gene
                     /label=BAT receptor
     variation       2067..2069
                     /label=N N2 K
                     /label=nonstandard type: mutation
                     /label=N to K
     misc_feature    2074..2097
                     /label=EP2 receptor
     misc_feature    2098..2916
                     /label=ICL3 Fromep2.3
                     /label=nonstandard type: gene
                     /label=truncate NE3.0 loop
     misc_feature    2098..2100
                     /label=N-Delta-41
     misc_feature    2098..2100
                     /label=Q5.73W
     misc_feature    2101..2103
                     /label=5.74
     misc_feature    2104..2106
                     /label=5.75
     misc_feature    2107..2109
                     /label=5.76
     misc_feature    2176..2898
                     /label=cpEGFP
     primer          complement(2427..2446)
                     /label=R-CAAX-gb-mcherry
                     /note="sequence: 
                     CACACTTTGTCTTTGACTTCTTTTTCTTCTTTTTACCATCCTTGTACAGCTCGTCCATG
                     "
     primer          2463..2484
                     /label=F-IRES-NcoI-gb-mcherry
                     /note="sequence: 
                     AAAACACGATGATAATATGGCCACAACCATGGTGAGCAAGGGCGAGGAG"
     misc_feature    2908..2910
                     /label=6.24
     misc_feature    2911..2913
                     /label=6.25
     misc_feature    2914..2916
                     /label=6.26
     misc_feature    2917..2955
                     /label=EP2 receptor
     misc_feature    2917..2919
                     /label=6.27
     misc_feature    2920..2922
                     /label=A6.28K
     misc_feature    2923..2925
                     /label=6.29
     misc_feature    2926..2928
                     /label=6.30
     misc_feature    2929..2931
                     /label=6.31
     misc_feature    2935..2976
                     /label=screened
     misc_feature    2956..2964
                     /label=nonstandard type: gene
                     /label=bat receptor
     variation       2971..2973
                     /label=C6 t-v
                     /label=nonstandard type: mutation
                     /label=T to V
     misc_feature    2986..2988
                     /label=P6.50
     primer          complement(3205..3231)
                     /label=Primer 1
                     /note="sequence: ttacaggctggattccatgtcattttg"
     primer          complement(3211..3261)
                     /label=AAV-batdp0.3-R
                     /note="sequence: 
                     TTGATTATCGATAAGCTTGATATCGAATTCttacaggctggattccatgtc"
     misc_feature    complement(3258..3620)
                     /label=Reverse primer region
                     /note="Reverse primer region"
     misc_feature    3258..3620
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
                     /label=WPRE
     terminator      3649..4127
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
                     /label=hGH_PA_terminator
     repeat_region   4392..4532
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4607..5062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    4973..4995
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
     promoter        5154..5182
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
     CDS             5224..6084
                     /codon_start=1
                     /label=ORF frame 1
                     /label=ORF frame 1
                     /translation="MPSSPKRARRKGLVIRLFL*VNVMIIMVS*TSGGTFRGNVRGTPI
                     CLFF*IHSNMYPLMRQ*P**MLQ*Y*KRKSMSIQHFRVALIPFFAAFCLPVFAHPETLV
                     KVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELT
                     AFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEAD"
     misc_feature    5224..6084
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
     rep_origin      6239..6858
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
     repeat_region   6905..7045
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
       61 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      121 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      181 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      241 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      301 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      361 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccactg gctgcagtac
      421 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
      481 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
      541 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
      601 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
      661 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
      721 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
      781 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
      841 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
      901 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
      961 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     1021 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     1081 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     1141 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     1201 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     1261 ccatttcagg tgtcgtgagg taccggatcc tctagagtcg acatggagac agacacactc
     1321 ctgctatggg tactgctgct ctgggttcca ggttccactg gtgacacaag tttgtacaaa
     1381 aaagttggca ccaccggtaa gcagatgttc taccgctgcc aaaacaccac ctcggtggag
     1441 aaagatcact cggcgacgat gggctgggtg ctcttcagcg ccggcctcct gggcaacctg
     1501 ctggcgctgg ggctgctggc gcgctcgggg ctggggtcgt gcccgctgcg ctcgccacgc
     1561 caggcgcctt cggtcttctg tgtgcttgtg tgtggcctga ccatcaccga cttgcttggc
     1621 aagtgcctct tgagccccgt cgtgctagca gcctacgcgc agaaccggag cctgtgggga
     1681 ctggtgtcca cgccgggcag ctctctgtgc caagccttcg cctttagtat gtctttcttc
     1741 ggcctcgcct cgacgctgca gctcctggcc atggccctgg agtgttggct atccctggga
     1801 caccccttct tctaccgaca gtacatcacc ctccgccggg gcgtgctggt ggcgcccgtc
     1861 gtgagcacct tctccttggc tttctgcgcg ctgcccttcg caggcttcgg aaagttcgtg
     1921 cagtactgcc ccggcacttg gtgcttcatc cagatggccc acgaggagcg ctcgccgtcg
     1981 gtgctgggct actcagtgct gtatgccagc atcctggcgc tgctggtcct cgccattgtg
     2041 ctgtgcaacc tgagcgccat gcgcaagctc taccgcatgc accgccgata tcggagatgg
     2101 ctcaacggct ggcctggcga gcccgcgccg gccgggccgc gcgacaccga cgcgctggac
     2161 ctggaggagg gaggaaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     2221 aacttccaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     2281 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     2341 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2401 gtgaccgccg ccgggatcac tgccggcatg gacgagctgt acaagggcgg taccggaggg
     2461 agcatggtga gaaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2521 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     2581 gagggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2641 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2701 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     2761 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2821 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2881 cacaagctgg agtacaacac cggagcagca gcacgcatga aggagcacat tgaccacctc
     2941 attctcctgg ctatcatgac cgtgctcttc gtgatgtgct ccctgccttt aacttatcgt
     3001 gcttactatg gagcatttaa agctgtcacc caggatggaa cctctgaaga agtggaagac
     3061 cttcgcgcct tgcgttttat atctgtgatc tcaattgtgg acccttggat tttcatcatt
     3121 ttcagaacct ctgtttttcg gatgtttttc cacaagattt tcatcaggcc tctgaggtat
     3181 agaaattggc atagcaatcc ctgccaaaat gacatggaat ccagcctgta agaattcgat
     3241 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3301 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3361 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3421 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3481 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3541 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3601 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3661 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3721 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3781 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3841 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc
     3901 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     3961 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     4021 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     4081 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     4141 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     4201 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     4261 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     4321 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac
     4381 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     4441 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     4501 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     4561 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     4621 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4681 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4741 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4801 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     4861 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4921 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     4981 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5041 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     5101 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5161 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5221 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     5281 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5341 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5401 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5461 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5521 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5581 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5641 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5701 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5761 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5821 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5881 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5941 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6001 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6061 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6121 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6181 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6241 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6301 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6361 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6421 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6601 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6841 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6901 agatcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
     6961 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
     7021 gccaactcca tcactagggg ttcct
//
