LOCUS       Exported                8730 bp ds-DNA     circular SYN 08-JAN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    St1Cas9_CNRZ_V2_ATM_G101
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, January 9, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     Ligation of : U6_sgRNA_CAG_hSth1Cas9_CNRZ1066_V2.xdna [8701 nt] : 
            (#BsmBI[356] / #BsmBI[343]) to Untitled Sequence #1 [21 nt] : (Uncut
            5'[0] / Uncut 3'[21])
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        79..341
                     /note="U6 promoter"
                     /note="color: #6495ed; direction: RIGHT"
     misc_feature    342..362
                     /note="ATM St1Cas9 target"
                     /note="color: #a6acb3"
     misc_feature    363..446
                     /note="crRNA:tracrRNA chimera"
                     /note="color: #8000ff; direction: RIGHT"
     promoter        451..2151
                     /note="CAG promoter"
                     /note="created_by=""User"" - label=""CAG promoter"" - 
                     18..1718"
                     /note="color: #fc8008; direction: RIGHT"
     misc_feature    2196..2205
                     /note="Kozak"
                     /note="created_by=""User"" - modified_by=""User"" - 
                     X-Transferred_From=""BPK2139; MSP680; MSP977"" - 
                     X-Transferred_Similarity=""100.00%"" - 
                     Transferred_From=""BPK2139, MSP1101, MSP680, MSP977"" - 
                     Transferred_Similarity=""100.00%"" - label=""Kozak"" - 
                     1763..1772"
                     /note="color: #fdcc65; direction: RIGHT"
     misc_feature    2202..2204
                     /note="ATG"
                     /note="color: #fb0106; direction: RIGHT"
     misc_feature    2211..2231
                     /note="SV40 NLS"
                     /note="color: #21fe06; direction: RIGHT"
     misc_feature    2256..5615
                     /note="St1Cas9 chimera"
                     /note="from AflII to NotI"
                     /note="color: #0f7ffe; direction: RIGHT"
     misc_feature    4343..4350
                     /note="Sbf1"
                     /note="color: #fc8008; direction: RIGHT"
     misc_feature    5136..5138
                     /note="V962L"
                     /note="note=""direct strand translation""translation=""L"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5139..5141
                     /note="S963K"
                     /note="note=""direct strand translation""translation=""K"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5151..5153
                     /note="A967T"
                     /note="note=""direct strand translation""translation=""T"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5172..5174
                     /note="T974A"
                     /note="note=""direct strand translation""translation=""A"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5397..5399
                     /note="M1049L"
                     /note="note=""direct strand translation""translation=""L"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5496..5498
                     /note="S1082G"
                     /note="note=""direct strand translation""translation=""G"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5508..5510
                     /note="K1086I"
                     /note="note=""direct strand translation""translation=""I"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5520..5522
                     /note="G1090A"
                     /note="note=""direct strand translation""translation=""A"""
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    5628..5648
                     /note="SV40 NLS"
                     /note="note=""direct strand 
                     translation""translation=""PKKKRKV"""
                     /note="color: #21fe06; direction: RIGHT"
     misc_feature    5655..5660
                     /note="STOPs"
                     /note="note=""direct strand translation""translation=""*"""
                     /note="color: #fb0106; direction: RIGHT"
     terminator      5684..6187
                     /note="RBGpA"
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
                     /note="color: #999999; direction: RIGHT"
     misc_feature    6246..6267
                     /note="M13R-20"
                     /note="color: #ff7f00; direction: RIGHT"
     promoter        complement(6282..6311)
                     /note="lac promoter"
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
                     /note="color: #f08080; direction: LEFT"
     unsure          6417..6596
                     /note="SV40 promoter"
                     /note="created_by=""User"" - label=""SV40 promoter"" - 
                     5921..6100"
                     /note="color: #ffd700"
     unsure          6452..6529
                     /note="SV40 origin"
                     /note="created_by=""User"" - Color=""#aa00aa"" - 
                     modified_by=""User"" - label=""SV40 origin"" - 5956..6033"
                     /note="color: #ffd700"
     terminator      6610..6741
                     /note="SV40 PA terminator"
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
                     /note="color: #f08080; direction: RIGHT"
     misc_feature    6797..6816
                     /note="pUC19 R"
                     /note="color: #ff7f00; direction: RIGHT"
     rep_origin      complement(6965..7584)
                     /direction=LEFT
                     /note="pBR322 origin"
                     /note="translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA
                     TYGKLT - 
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK 
                     - 
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN 
                     - 
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH 
                     - MVLLEFVTAAGI"
                     /note="color: #84a4c0"
     CDS             complement(7742..8599)
                     /note="AmpR"
                     /note="vntifkey=""4"" - label=AmpR - 
                     complement(1629..2486)"
                     /note="color: #ff4500; direction: LEFT"
     misc_feature    8600..8664
                     /note="bla\promoter"
                     /note="vntifkey=""21"" - label=bla\promoter - 2487..2551"
                     /note="color: #dc143c; direction: RIGHT"
     promoter        complement(8641..8669)
                     /note="amp promoter"
                     /note="translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQL
                     GARVGY - 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     - 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     - 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     - LRSALPAGWFIA"
                     /note="color: #f08080; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt
       61 cgactggatc cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat
      121 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
      181 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      241 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      301 atttcttggc tttatatatc ttgtggaaag gacgaaacac cattaagtac tagactcatg
      361 gtgtctttgt actctggtac cagaagctac aaagataagg cttcatgccg aaatcaacac
      421 cctgtcattt tatggcaggg tgtttttttt actagttatt aatagtaatc aattacgggg
      481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc
      661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      781 cagtacatct acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      901 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc
      961 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1081 gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct
     1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt
     1261 ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg
     1321 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg
     1381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag
     1441 cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg
     1501 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc
     1561 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1621 tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1681 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg
     1741 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     1801 gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc
     1861 gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg
     1921 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg
     1981 ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2041 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2101 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc
     2161 agtcgacggt accgcgggcc cgggatccac cggtcgccac catgggcgcc ccaaagaaga
     2221 agcggaaggt cggtatccac ggagtcccag cagccagcga cctggtgctg ggcctggaca
     2281 tcggcatcgg cagcgtgggc gtgggcatcc tgaacaaggt gaccggcgag atcatccaca
     2341 agaacagtcg catcttccct gctgctcagg ctgagaacaa cctggtgcgc cgcaccaacc
     2401 gccagggtcg ccggcttgct cgccgcaaga agcaccggcg cgtgcgcctg aaccgcctgt
     2461 tcgaggagag cggcctgatc accgacttca ccaagatcag catcaacctg aacccctacc
     2521 agctgcgcgt gaagggcctg accgacgagc tgagcaacga ggagctgttc atcgccctga
     2581 agaacatggt gaagcaccgc ggcatcagct acctggacga cgccagcgac gacggcaaca
     2641 gcagcgtggg cgactacgcc cagatcgtga aggagaacag caagcagctg gagaccaaga
     2701 cccccggcca gatccagctg gagcgctacc agacctacgg ccagctgcgc ggcgacttca
     2761 ccgtggagaa ggacggcaag aagcaccgcc tgatcaacgt gttccccacc agcgcctacc
     2821 gcagcgaggc cctgcgcatc ctgcagaccc agcaggagtt caacccccag atcaccgacg
     2881 agttcatcaa ccgctacctg gagatcctga ccggcaagcg caagtactac cacggccccg
     2941 gcaacgagaa gagccgcacc gactacggcc gctaccgcac cagcggcgag accctggaca
     3001 acatcttcgg catcctgatc ggcaagtgca ccttctaccc cgacgagttc cgcgccgcca
     3061 aggccagcta caccgcccag gagttcaatc tgctgaacga cctgaacaac ctgaccgtgc
     3121 ccaccgagac caagaagctg agcaaggagc agaagaacca gatcatcaac tacgtgaaga
     3181 acgagaaggc tatgggcccc gccaagctgt tcaagtacat cgccaagctg ctgagctgcg
     3241 acgtggccga catcaagggc taccgcatcg acaagagcgg caaggccgag atccacacct
     3301 tcgaggccta ccgcaagatg aagaccctgg agaccctgga catcgagcag atggaccgag
     3361 agaccctgga caagctggcc tacgtgctga ccctgaacac cgagcgcgag ggcatccagg
     3421 aggccctgga gcacgagttc gccgacggca gcttcagcca gaaacaggtg gacgagctgg
     3481 tgcagttccg caaggccaac agcagcatct tcggcaaggg ctggcacaac ttcagcgtga
     3541 agctgatgat ggagctgatc cccgagctgt acgagaccag cgaggagcag atgaccatcc
     3601 tgacccgcct gggcaagcag aagaccacca gcagcagcaa caagaccaag tacatcgacg
     3661 agaagctgct gaccgaggag atctacaacc ccgtggtggc caagagcgtg cgccaggcca
     3721 tcaagatcgt gaacgccgcc atcaaggagt acggcgactt cgacaacatc gtgatcgaga
     3781 tggcccgcga gaccaacgag gacgacgaga agaaggccat ccagaagatc cagaaggcca
     3841 acaaggacga gaaggacgcc gccatgctga aggccgccaa ccagtacaac ggcaaggccg
     3901 agctgcccca cagcgtgttc cacggccaca agcagctggc caccaagatc cgcctgtggc
     3961 accagcaggg cgagcgctgc ctgtacaccg gcaagaccat cagcatccac gacctgatca
     4021 acaacagcaa ccagttcgag gtggaccaca tcctgcccct gagcatcacc ttcgacgaca
     4081 gcctggccaa caaggtgctg gtgtacgcca ccgccaacca ggagaagggc cagcgcaccc
     4141 cctaccaggc cctggacagc atggacgacg cctggagctt ccgcgagctg aaggccttcg
     4201 tgcgcgagag caagaccctg agcaacaaga agaaggagta tctgctgacc gaggaggaca
     4261 tcagcaagtt cgacgtgcgc aagaagttca tcgagcgcaa cctggtggac acccgctacg
     4321 ccagccgcgt ggtgctgaac gccctgcagg agcacttccg cgcccacaag atcgacacca
     4381 aggtgagcgt ggtgcgcggc cagttcacca gccagctgcg ccgccactgg ggcatcgaga
     4441 agacccgcga cacctaccac caccacgccg tggacgccct gatcattgcg gcttctagcc
     4501 agctgaacct gtggaagaag cagaagaaca ccctggtgag ctacagcgag gaccagctgc
     4561 tggacatcga gaccggcgag ctgatcagcg acgacgagta caaggagagc gtgttcaagg
     4621 ccccctacca gcacttcgtg gacaccctga agagcaagga gttcgaggac agcatcctgt
     4681 tcagctacca ggtggacagc aagttcaacc gcaagatcag cgacgccacc atctacgcca
     4741 cccgccaggc caaggtgggc aaggacaagg ccgacgagac ctacgtgctg ggcaagatca
     4801 aggacatcta cacccaggac ggctacgacg ccttcatgaa gatctacaag aaggacaaga
     4861 gcaagttcct gatgtaccgc cacgaccccc agaccttcga gaaggtgatc gagcccatcc
     4921 tggagaacta ccccaacaag cagatcaacg agaaaggcaa ggaggtgccc tgcaacccct
     4981 tcctgaagta caaggaggag cacggctaca tccgcaagta cagcaagaag ggcaacggcc
     5041 ccgagatcaa gagcctgaag tactacgaca gcaagctggg caaccacatc gacatcaccc
     5101 ccaaggacag caacaacaag gtggtgctgc agagccttaa gccctggcgc accgacgtgt
     5161 acttcaacaa ggccaccggc aagtacgaga tcctggggct gaagtacgcc gatctgcagt
     5221 ttgagaaagg cacaggcacc tacaagatca gccaggagaa gtacaacgac atcaagaaga
     5281 aggagggcgt ggacagcgac agcgagttca agttcaccct gtacaagaac gaccttctgc
     5341 tggtgaagga caccgagacc aaggagcaac agctgttccg cttcctgagc cgcaccctgc
     5401 ccaagcagaa gcactacgtg gagctgaagc cctacgacaa gcagaagttc gagggcggcg
     5461 aggccctgat caaggtgctg ggcaacgtgg ccaacggcgg ccagtgcatc aagggcctgg
     5521 ccaagagcaa catcagcatc tacaaggtgc gcaccgacgt gctgggcaac cagcacatca
     5581 tcaagaacga gggcgacaag cccaagttgg acttcagcag ggctgacccc aagaagaaga
     5641 ggaaggtggg atcctgataa gaattcgcgg ccgcactcct caggtgcagg ctgcctatca
     5701 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     5761 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     5821 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     5881 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     5941 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     6001 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     6061 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     6121 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     6181 tcttctctta tgaagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     6421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6601 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6721 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6781 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7141 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7381 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8701 cgcgcacatt tccccgaaaa gtgccacctg
//
