LOCUS       CN2602_rAAV-eHGT_769m-minBglobin-SYFP2-WPRE3-BGHpA        4785 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...1np-c05p9wg
VERSION     urn.local...1np-c05p9wg
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2602_rAAV-eHGT_769m-minBglobin-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 802 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2187 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2136 0 2147 0 0 
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COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2134 0 2145 0 0 
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COMMENT        #13=(CFSignal (CObList) "SYFP2" 21 0 0 803 1522 0 (CStringList) 
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COMMENT                          "Gibson/Homology Cloning ligation product." 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/urn.local...1np-c05p9wg\">urn.local...1np-c05p9wg</a>" 
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COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    136^137
                     /Original_Bases="AAGAGGAGTGGCCAACTCCATCACTAGGGG"
                     /vntifkey="21"
                     /label=AAGAGGAGTGGCCAACTCCATCACTAGGGG
                     /note="Geneious type: Editing History Deletion"
     misc_feature    156..703
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_769m
     misc_feature    710..762
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    727^728
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCC
                     AGNNNTNNNNNNNNNNNNN"
                     NNNNNNNNNN"
                     /vntifkey="21"
                     /label=AGGGCAGAGCCATCTATTGCTTACATTTGCTTCTGGGATCCAGNNNTNNN
                     /note="Geneious type: Editing History Deletion"
     misc_feature    803..1522
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    1557..1802
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1816..2019
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2048..2188
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2280..2586
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3105..3962
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4113..4780
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1164 a      1251 c      1216 g      1154 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaccag ctggggaatg agtgtttaga 
      181 tacttgagac tatgggagac agttatcatt aaaactcaca aaaccagaaa gagggaatcc 
      241 aatttctaca aaataaagat aatttattca gaagccaatt atgaatgtca gatttcacca 
      301 aatactgtgc ttcaatgtgc taacagtttt acagacacag aacaaagaaa gctataatcc 
      361 aaaacacctt ttatcaaatc cactaatggg aacatcagac agattatata gcacaatagg 
      421 gaaatctctg ctaggaaggc tctagaccgg tgactttcca gcctctttgc aatcattcaa 
      481 aaactaatca agctattcat ggtggctcat gccttgaatc cccagcattt agaagggatt 
      541 aaacaagatc taatcagttg agtagccttg taggaagttc agtacagtag aaggtgtggt 
      601 tactttctgg gaatttttat tcacataagc aatttagaat aatcaacatg cctttgagga 
      661 atatgaaaaa cttaaaattt ctcttgtaga aaggcttgtg ggtgagctcg ggctgggcat 
      721 aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccag atctttcgaa 
      781 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 
      841 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 
      901 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa 
      961 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcgtgc agtgcttcgc 
     1021 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 
     1081 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 
     1141 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 
     1201 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 
     1261 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga 
     1321 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 
     1381 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa 
     1441 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 
     1501 catggacgag ctgtacaagt aagtcgacgg cgcgccgcgg ccgcgaattc gatatcataa 
     1561 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 
     1621 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 
     1681 ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat 
     1741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     1801 ggctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     1861 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     1921 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     1981 acagcaaggg ggaggattgg gaagacaata gcaggcatga gatctcacgt gcggaccgag 
     2041 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc 
     2101 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg 
     2161 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat 
     2221 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg 
     2281 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     2341 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     2401 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     2461 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     2521 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     2581 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt 
     2641 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 
     2701 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt 
     2761 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 
     2821 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 
     2881 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg 
     2941 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 
     3001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     3061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 
     3121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 
     3181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 
     3241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 
     3301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 
     3361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 
     3421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 
     3481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 
     3541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 
     3601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 
     3661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 
     3721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 
     3781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 
     3841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 
     3901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 
     3961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 
     4021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     4081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     4141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     4201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     4261 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     4321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     4381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     4441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     4501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     4561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     4621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     4681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     4741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt 
//
