LOCUS       CN3450_pAAV-eHGT_140h-minBglobin-iCre-4X2C-WPRE3-BGHpA        5078 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...6b-dt5buv4
VERSION     urn.local...6b-dt5buv4
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN3450_pAAV-eHGT_140h-minBglobin-iCre-4X2C-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN3450_pAAV-eHGT_140h-minBglobin-iCre-4X2C-WPRE3-BGHpA" 0 5078 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
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COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 592 0 3017 0 3514 0 4760 
COMMENT            0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 9 41 0 52 0 467 0 1346 0 
COMMENT            1588 0 2103 0 2427 0 2438 0 2894 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 523 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1845 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 548 0 1574 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2480 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 5 43 0 54 0 1348 0 2429 0 
COMMENT            2440 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 5 41 0 52 0 1346 0 2427 0 
COMMENT            2438 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 2 679 0 818 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3267 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 1840 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 4 865 0 2943 1 3140 1 
COMMENT             3882 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 46 38 0 143 1 147 0 288 0 
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COMMENT             1 3671 0 3792 1 3838 0 4029 1 4120 1 4482 0 4491 1 4626 0 4736 1 
COMMENT             4857 1 4876 1 5003 1 5031 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3000 0 3584 0 3957 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 3 297 0 3520 0 4532 1 0 
COMMENT             "") #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 4 1631 0 1657 0 1683 0 
COMMENT             1709 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 24 12 0 300 0 466 0 541 0 
COMMENT             744 0 855 0 1192 0 1230 0 1752 0 1780 0 1808 0 1836 0 2471 0 2700 0 
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COMMENT             0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 15 518 1 531 0 716 1 1004 
COMMENT             1 1149 1 1252 0 1369 0 3547 0 3551 1 3868 0 4331 1 4332 0 4428 1 
COMMENT             4430 0 4516 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1353 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 1610 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4011 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 1169 0 1517 0 2327 0 
COMMENT             3822 0 4044 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2487 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 840 0 2313 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 835 0 2308 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 21 21 0 325 1 717 1 851 1 
COMMENT             1073 1 1302 1 1443 1 1571 1 1927 1 2045 1 2458 1 2480 0 2617 0 3141 
COMMENT             1 3752 1 4143 0 4446 1 4652 1 4655 1 4745 0 5077 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 17 133 0 297 1 505 0 648 
COMMENT             1 841 0 1011 1 1132 1 1235 1 1495 0 1588 1 2147 0 2349 1 2772 1 
COMMENT             2804 0 3729 1 4016 1 4140 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 3 2043 0 2760 0 4574 1 0 
COMMENT             "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 3 2043 0 2760 0 4575 1 0 
COMMENT             "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 4 312 1 1928 1 3349 0 
COMMENT             4876 0 0 "") #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 9 131 0 203 0 542 0 2128 0 
COMMENT             2350 0 2634 0 3993 0 4328 0 4581 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 3 529 0 874 0 2109 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 1577 0 4146 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 545 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 1 4117 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3582 0 4450 0 4691 1 
COMMENT             4989 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2424 0 2484 0 3264 
COMMENT             0 3646 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 5 1622 1 1648 1 1674 1 
COMMENT             1700 1 4120 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 14 338 1 615 1 695 1 942 
COMMENT             1 1027 1 2234 0 2483 1 2519 0 3035 1 3125 0 3488 1 3733 0 3928 1 
COMMENT             4980 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 13 23 1 98 0 767 1 1553 
COMMENT             1 1767 0 2229 1 2387 1 2462 0 3038 0 3699 0 4216 1 4382 1 4791 1 0 
COMMENT             "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 939 0 1984 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 7 472 1 952 1 1736 0 1764 
COMMENT             0 1792 0 1820 0 4770 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 2 738 0 850 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 11 391 1 579 0 1558 1 
COMMENT             1622 1 1648 1 1674 1 1700 1 3149 0 3191 1 3344 1 4120 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3149 0 3191 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 7 430 0 682 0 802 0 897 
COMMENT             1 1197 1 1477 0 1612 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 1 1154 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 13 24 1 97 0 768 1 1554 
COMMENT             1 1766 0 2230 1 2388 1 2461 0 3037 0 3698 0 4217 1 4383 1 4792 1 0 
COMMENT             "") #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3241 0 3346 0 4354 0 0 
COMMENT             "") 
COMMENT        #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 3 590 0 611 0 812 0 0 
COMMENT             "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 4 1724 1 2334 1 2643 0 
COMMENT             3344 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 1067 1 1091 1 3952 0 
COMMENT             4126 1 0 "") #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 11 1572 0 1900 0 2876 0 
COMMENT             3541 0 3711 1 3980 0 4023 0 4141 0 4547 0 4659 1 4672 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 1610 0 2379 
COMMENT             0 2464 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 3 3210 1 3517 0 4901 0 0 
COMMENT             "") #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 536 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 1 1221 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 627 0 786 0 833 1 954 
COMMENT             0 1407 0 1487 1 1599 0 3117 1 3740 0 4027 0 4208 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 24 526 0 532 0 724 0 877 0 
COMMENT             1012 0 1157 0 1247 0 1364 0 1388 0 1698 0 2112 0 3506 0 3542 0 3559 
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COMMENT             4511 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 23 696 0 726 0 735 0 820 0 
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COMMENT             1428 0 1461 0 1473 0 1555 0 2011 0 2178 0 4915 0 4928 0 5049 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 19 17 0 102 0 153 0 1612 0 
COMMENT             1617 0 2381 0 2466 0 2559 0 2583 0 2603 0 2979 0 3090 0 3193 0 3195 
COMMENT             0 3295 0 3627 0 4120 0 4450 0 5031 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 39 12 0 15 0 40 0 146 0 
COMMENT             149 0 341 0 733 0 867 0 1089 0 1318 0 1459 0 1587 0 1617 0 1620 0 
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COMMENT             4462 0 4668 0 4671 0 4736 0 4879 0 5034 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 2 3767 0 3787 1 0 "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 41 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 298 0 336 0 439 0 543 0 608 0 677 0 742 0 809 0 
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COMMENT             2381 0 2385 0 2389 0 2458 0 2462 0 2466 0 2476 0 2627 0 2641 0 2684 
COMMENT             0 3060 0 4073 0 4464 0 5024 0 5061 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 6 1137 1 2041 0 2230 0 2800 
COMMENT             0 3533 0 3934 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 24 527 0 533 0 725 0 878 0 
COMMENT             1013 0 1158 0 1248 0 1365 0 1389 0 1699 0 2113 0 3507 0 3543 0 3560 
COMMENT             0 3818 0 3864 0 3882 0 4223 0 4328 0 4340 0 4418 0 4426 0 4437 0 
COMMENT             4512 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 5 259 0 801 0 2555 0 3028 0 
COMMENT             3704 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 18 291 0 549 0 660 0 861 0 
COMMENT             1467 0 1500 0 1575 0 1961 0 2316 0 3158 0 3242 0 3347 0 3740 0 3776 
COMMENT             0 3854 0 3864 0 4355 0 5075 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 100 12 0 37 0 51 0 82 0 118 
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COMMENT             2337 0 2365 0 2401 0 2432 0 2446 0 2471 0 2686 0 2700 0 2714 0 2795 
COMMENT             0 2899 0 2937 0 2957 0 3058 0 3062 0 3102 0 3135 0 3154 0 3208 0 
COMMENT             3795 0 3833 0 3896 0 3906 0 3996 0 4062 0 4071 0 4075 0 4101 0 4142 
COMMENT             0 4154 0 4517 0 4546 0 4589 0 4600 0 4665 0 4744 0 4769 0 4774 0 
COMMENT             4820 0 4910 0 5008 0 5034 0 5052 0 5063 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 15 32 0 84 0 420 0 776 0 
COMMENT             1562 0 1753 0 2238 0 2396 0 2448 0 3024 0 3259 0 3685 0 4225 0 4391 
COMMENT             0 4800 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 24 525 0 531 0 723 0 876 0 
COMMENT             1011 0 1156 0 1246 0 1363 0 1387 0 1697 0 2111 0 3505 0 3541 0 3558 
COMMENT             0 3816 0 3862 0 3880 0 4221 0 4326 0 4338 0 4416 0 4424 0 4435 0 
COMMENT             4510 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 4 313 0 3608 0 4300 0 4319 
COMMENT             0 0 "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 144 0 2335 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 2 559 1 3387 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 4 2045 0 4044 0 4872 0 
COMMENT             5018 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 4 297 0 3520 0 4117 0 
COMMENT             4532 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 18 40 0 41 0 51 0 52 0 77 
COMMENT             0 1292 0 1345 0 1346 0 2401 0 2426 0 2427 0 2437 0 2438 0 3112 0 
COMMENT             3147 0 3648 0 3999 0 4695 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 466 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 4 972 0 1206 0 1350 0 
COMMENT             3206 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1853 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 9 537 0 1590 0 1841 0 
COMMENT             1850 0 2105 0 2116 0 2753 0 3533 0 4977 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 18 290 0 548 0 659 0 860 0 
COMMENT             1466 0 1499 0 1574 0 1960 0 2315 0 3157 0 3241 0 3346 0 3739 0 3775 
COMMENT             0 3853 0 3863 0 4354 0 5074 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 6 1138 0 2063 0 2579 1 
COMMENT             2648 0 3079 0 3089 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 15 852 0 975 0 1172 0 1209 
COMMENT              0 1352 0 1353 0 1520 0 1574 0 2330 0 2796 0 3209 0 3825 0 4047 0 
COMMENT              4064 0 4143 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 39 10 0 13 0 38 0 144 0 
COMMENT              147 0 339 0 731 0 865 0 1087 0 1316 0 1457 0 1585 0 1615 0 1618 0 
COMMENT              1725 0 1941 0 2043 0 2059 0 2335 0 2338 0 2444 0 2469 0 2472 0 
COMMENT              2570 0 2584 0 2606 0 3046 0 3155 0 3442 0 3671 0 3766 0 3793 0 
COMMENT              4132 0 4460 0 4666 0 4669 0 4734 0 4877 0 5032 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 11 634 0 793 0 820 1 961 
COMMENT              0 1414 0 1474 1 1606 0 3104 1 3747 0 4034 0 4215 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 2 1090 0 3963 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 5 144 1 144 0 2335 1 
COMMENT              2335 0 3793 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 34 37 0 82 0 118 0 146 0 
COMMENT              679 0 818 0 851 0 881 0 973 0 979 0 1057 0 1207 0 1351 0 1372 0 
COMMENT              1426 0 1436 0 1471 0 1573 0 1620 0 1727 0 2337 0 2365 0 2401 0 
COMMENT              2446 0 2795 0 2937 0 3208 0 3795 0 4062 0 4142 0 4600 0 5034 0 
COMMENT              5052 0 5063 0 0 "") 
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COMMENT                              "BbsI: 2 sites") 8 12 "GAAGACNNNNNNN" 
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COMMENT                             "GCAGCNNNNNNNNNNNNN" "CGTCGNNNNNNNNNNNNN") 
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COMMENT                             "CCATCNNNNNN" "GGTAGNNNNNN") 
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COMMENT                                             " N3: 4574 (on complementary strand)" 
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COMMENT                             "ACGGCNNNNNNNNNNNNNNN" "TGCCGNNNNNNNNNNNNNNN") 
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COMMENT                                             " N3: 4575 (on complementary strand)" 
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COMMENT                             "ACGGCNNNNNNNNNNNNNN" "TGCCGNNNNNNNNNNNNNN") 
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COMMENT                                             " N1: 312 (on complementary strand)" 
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COMMENT                                             " N2: 1928 (on complementary strand)" 
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COMMENT                                       #411=(CLinePar 
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COMMENT                              "BciVI: 4 sites") 12 11 "GTATCCNNNNNNN" 
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COMMENT                               (CParagraph 104593448 (8 0) 1 1 1 0 178) 
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COMMENT                               (CParagraph 104593512 (8 0) 1 1 1 0 178) 
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COMMENT                              "BglII: 3 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                             "CGANTCG") 
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COMMENT                               (CParagraph 104593128 (8 0) 1 1 1 0 178) 
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COMMENT                                       #425=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #45# 2) 1 2 2 0 
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COMMENT                              "BmrI: 2 sites") 11 10 "ACTGGGNNNNNN" 
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COMMENT                              "BmtI: 1 site") 5 1 "GCTAGC" "CGATCG") 
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COMMENT                              "BpmI: 1 site") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                             " N3: 4691 (on complementary strand)" 
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COMMENT                              "BpuEI: 4 sites") 22 20 "CTTGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaHI: 5 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                                             " N3: 1674 (on complementary strand)" 
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COMMENT                                             " N4: 1700 (on complementary strand)" 
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COMMENT                              "BsaI: 5 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
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COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
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COMMENT                                             " N2: 1984 (on complementary strand)" 
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COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
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COMMENT                                       #462=(CLinePar 
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COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
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COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
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COMMENT                                       #468=(CLinePar 
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COMMENT                                             " N2: 3191 (on complementary strand)" 
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COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
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COMMENT                              "BsmI: 1 site") 7 5 "GAATGCNN" "CTTACGNN") 
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COMMENT                               (CObjectList)) "BspCNI: 13 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
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COMMENT                                       #478=(CLinePar 
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COMMENT                                       #479=(CLinePar 
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COMMENT                              "BspHI: 3 sites") 1 5 "TCATGA" "AGTACT") 
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COMMENT                                       #483=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #64# 2) 1 2 2 0 
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COMMENT                                       #484=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #64# 3) 1 2 2 0 
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COMMENT                              "BspMI: 3 sites") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                               (CParagraph 104593992 (8 0) 1 1 1 0 178) 
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COMMENT                                             " N1: 1724 (on complementary strand)" 
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COMMENT                                       #488=(CLinePar 
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COMMENT                                             " N2: 2334 (on complementary strand)" 
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COMMENT                                       #489=(CLinePar 
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COMMENT                                       #490=(CLinePar 
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COMMENT                              "BsrBI: 4 sites") 3 3 "CCGCTC" "GGCGAG") 
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COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #66# 0) 1 2 2 0 180) 
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COMMENT                                       #493=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #66# 1) 1 2 2 0 
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COMMENT                                             " N1: 1067 (on complementary strand)" 
COMMENT                                             1) 
COMMENT                                       #494=(CLinePar 
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COMMENT                                             " N2: 1091 (on complementary strand)" 
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COMMENT                                       #495=(CLinePar 
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COMMENT                                       #496=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #66# 4) 1 2 2 0 
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COMMENT                                             " N4: 4126 (on complementary strand)" 
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COMMENT                             "GCAATGNNN" "CGTTACNNN") 
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COMMENT                               (CParagraph 104594024 (8 0) 1 1 1 0 178) 
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COMMENT                               (CParagraph 104594408 (8 0) 1 1 1 0 178) 
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COMMENT                                       #504=(CLinePar 
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COMMENT                                       #505=(CLinePar 
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COMMENT                              "BssHII: 5 sites") 1 5 "GCGCGC" "CGCGCG") 
COMMENT                       #506=(CRSFolderPar 
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COMMENT                                      (CParagraph 0 (10 #70# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #508=(CLinePar 
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COMMENT                                             " N1: 3210 (on complementary strand)" 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..462
                     /created_by="refugio.martinez"
                     /vntifkey="21"
                     /label=eHGT_140h
     misc_feature    469..521
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    546..1604
                     /vntifkey="21"
                     /label=iCre
     misc_feature    complement(650..670)
                     /vntifkey="21"
                     /label=iCre-seq-R\(JT)
     misc_feature    1624..1644
                     /vntifkey="21"
                     /label=miR128\recognition\sequence
     misc_feature    1650..1670
                     /vntifkey="21"
                     /label=miR128\recognition\sequence
     misc_feature    1676..1696
                     /vntifkey="21"
                     /label=miRNA128\recognition\sequence
     misc_feature    1702..1722
                     /vntifkey="21"
                     /label=miRNA128\recognition\sequence
     misc_feature    1731..1753
                     /vntifkey="21"
                     /label=miR221\recognition\sequence
     misc_feature    1759..1781
                     /vntifkey="21"
                     /label=miR221\recognition\sequence
     misc_feature    1787..1809
                     /vntifkey="21"
                     /label=miR221\recognition\sequence
     misc_feature    1815..1837
                     /vntifkey="21"
                     /label=miR221\recognition\sequence
     misc_feature    1856..2101
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2115..2318
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2341..2481
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2573..2879
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3398..4255
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4406..5073
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1202 a      1288 c      1352 g      1236 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtggttg tgtgtgaaga ttaagccctt 
      181 tcttgctcta tgtatttgag cctagaaaaa gacacactcc caagaaatta gaataaaggg 
      241 aaaaatgact ttgagatgta ctttcttaaa ataactgaag taatgaccgc atgagccagc 
      301 tgatgggttt ttaaatggat acgtttctat cagcctgctg cttgatgcca gagccaaata 
      361 tatatggagt gtattatatc atatcctgta ggcaggagac tgttggggca ttgggggact 
      421 tagagaggtg aagtggcagg cttggcacag gaattaacag cagagctcgg gctgggcata 
      481 aaagtcaggg cagagccatc tattgcttac atttgcttct gggatccaga tctttcgaag 
      541 ctagcaccat ggtgcccaag aagaagagga aagtctccaa cctgctgact gtgcaccaaa 
      601 acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca 
      661 tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca 
      721 gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct gaacctgagg 
      781 atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag accatccaac 
      841 agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc ccttctgact 
      901 ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat gctggggaga 
      961 gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga tccctgatgg 
     1021 agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt gcctacaaca 
     1081 ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc cgcaccgatg 
     1141 gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca gctggtgtgg 
     1201 agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct gtgtctggtg 
     1261 tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt gtggctgccc 
     1321 cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag gccacccacc 
     1381 gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg tctggccact 
     1441 ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc cctgaaatca 
     1501 tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga aacctggact 
     1561 ctgagactgg ggccatggtg aggctgctcg aggatgggga ctaatgaggc gcgccgcggc 
     1621 cttaaagaga ccggttcact gtgacagtaa aagagaccgg ttcactgtga gaatgaaaga 
     1681 gaccggttca ctgtgatcgg aaaagagacc ggttcactgt gagcggcctt gaaacccagc 
     1741 agacaatgta gctcagtaga aacccagcag acaatgtagc tgaatggaaa cccagcagac 
     1801 aatgtagctt cggagaaacc cagcagacaa tgtagctgtc gacgaattcg atatcataat 
     1861 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     1921 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     1981 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc 
     2041 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     2101 gctcgagaga tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 
     2161 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
     2221 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 
     2281 cagcaagggg gaggattggg aagacaatag caggcatgca cgtgcggacc gagcggccgc 
     2341 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 
     2401 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 
     2461 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg 
     2521 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag 
     2581 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     2641 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     2701 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     2761 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     2821 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     2881 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta 
     2941 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 
     3001 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 
     3061 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 
     3121 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 
     3181 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 
     3241 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 
     3301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     3361 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     3421 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     3481 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     3541 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     3601 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     3661 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     3721 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     3781 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     3841 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     3901 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     3961 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     4021 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     4081 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     4141 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     4201 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     4261 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     4321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     4381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     4441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     4501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     4561 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     4621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     4681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     4741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     4801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     4861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     4921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     4981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     5041 acggttcctg gccttttgct ggccttttgc tcacatgt 
//
