LOCUS       CN4360_pAAV-3xcore2_eHGT_779m-minBglobin-jGCaMP8s-WPRE3-BGHpA        5344 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...ls-el4vade
VERSION     urn.local...ls-el4vade
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN4360_pAAV-3xcore2_eHGT_779m-minBglobin-jGCaMP8s-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN4360_pAAV-3xcore2_eHGT_779m-minBglobin-jGCaMP8s-WPRE3-BGHpA" 0 
COMMENT       5344 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1668571495 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5344 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3283 0 3780 0 5026 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 10 41 0 52 0 217 0 411 0 
COMMENT            605 0 742 0 2369 0 2693 0 2704 0 3160 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 798 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2036 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2111 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 7 0 1793 0 2746 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2695 0 2706 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2693 0 2704 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3533 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1195 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 10 1018 0 1168 1 1216 
COMMENT             1 1249 1 1570 1 1726 1 1774 1 3209 1 3406 1 4148 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 57 38 0 143 1 147 0 181 1 
COMMENT             375 1 569 1 881 0 978 0 998 1 1151 0 1154 0 1193 1 1428 0 1469 0 
COMMENT             1536 0 1575 0 1755 1 2101 0 2103 1 2107 0 2296 1 2309 0 2322 0 2591 
COMMENT             1 2600 1 2604 0 2709 1 2759 1 2785 1 2798 0 2835 1 2849 1 2852 1 
COMMENT             2880 0 2907 0 3312 0 3338 0 3348 0 3387 0 3561 1 3608 0 3707 1 3816 
COMMENT             1 3893 1 3937 0 4058 1 4104 0 4295 1 4386 1 4748 0 4757 1 4892 0 
COMMENT             5002 1 5123 1 5142 1 5269 1 5297 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3266 0 3850 0 4223 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 7 934 0 1363 0 1407 1 
COMMENT             1606 0 1682 0 3786 0 4798 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 26 12 0 741 0 816 0 1008 0 
COMMENT             1182 0 1230 0 1263 0 1335 0 1368 0 1584 0 1632 0 1740 0 1788 0 1959 
COMMENT             0 2737 0 2966 0 3223 0 3401 0 3420 0 4099 0 4162 0 4262 0 4783 0 
COMMENT             5040 0 5086 0 5176 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 11 793 1 806 0 1168 0 
COMMENT             3813 0 3817 1 4134 0 4597 1 4598 0 4694 1 4696 0 4782 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2096 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4277 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 1131 0 1650 0 2593 0 
COMMENT             4088 0 4310 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2753 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 870 0 2579 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 865 0 2574 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 27 21 0 199 1 212 1 393 1 
COMMENT             406 1 587 1 600 1 1017 0 1043 1 1046 1 1355 1 1461 0 1775 1 1963 0 
COMMENT             2193 1 2311 1 2724 1 2746 0 2883 0 3407 1 4018 1 4409 0 4712 1 4918 
COMMENT             1 4921 1 5011 0 5343 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 19 133 0 780 0 845 0 900 
COMMENT             1 1045 0 1258 0 1510 0 1711 1 1854 1 1924 1 2032 1 2038 1 2413 0 
COMMENT             2615 1 3038 1 3070 0 3995 1 4282 1 4406 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 14 868 1 969 0 1011 0 
COMMENT             1062 0 1284 0 1296 0 1344 0 1425 0 1536 0 1626 0 1842 0 2309 0 3026 
COMMENT             0 4840 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 14 869 1 969 0 1011 0 
COMMENT             1062 0 1284 0 1296 0 1344 0 1425 0 1536 0 1626 0 1842 0 2309 0 3026 
COMMENT             0 4841 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2194 1 3615 0 5142 0 
COMMENT             0 "") 
COMMENT        #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1799 0 2018 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 8 131 0 817 0 2394 0 2616 0 
COMMENT             2900 0 4259 0 4594 0 4847 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 828 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 5 249 0 443 0 637 0 804 0 
COMMENT             2375 0 0 "") #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4412 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 820 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1159 0 1654 0 4383 0 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 4 3848 0 4716 0 4957 1 
COMMENT             5255 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2690 0 2750 0 3530 
COMMENT             0 3912 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1763 0 4386 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 836 0 964 0 1311 1 
COMMENT             1585 0 1600 0 1745 1 1806 1 2500 0 2749 1 2785 0 3301 1 3391 0 3754 
COMMENT             1 3999 0 4194 1 5246 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 15 23 1 98 0 188 1 382 
COMMENT             1 576 1 1069 1 1835 1 2495 1 2653 1 2728 0 3304 0 3965 0 4482 1 
COMMENT             4648 1 5057 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 5 205 0 399 0 593 0 
COMMENT             1240 0 2250 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 747 1 1740 1 5036 0 0 
COMMENT             "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 1024 0 1338 1 1435 0 
COMMENT             1970 0 0 "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 11 157 1 303 0 351 1 497 
COMMENT             0 545 1 691 0 1763 0 3415 0 3457 1 3610 1 4386 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3415 0 3457 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 3 1734 0 1758 0 1897 0 0 
COMMENT             "") #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 15 24 1 97 0 189 1 383 
COMMENT             1 577 1 1070 1 1836 1 2496 1 2654 1 2727 0 3303 0 3964 0 4483 1 
COMMENT             4649 1 5058 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3507 0 3612 0 4620 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1652 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2600 1 2909 0 3610 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 1941 0 4218 0 4392 1 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1184 0 2070 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 10 2166 0 3142 0 3807 0 
COMMENT             3977 1 4246 0 4289 0 4407 0 4813 0 4925 1 4938 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 2096 0 2645 
COMMENT             0 2730 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1390 1 3476 1 3783 0 
COMMENT             5167 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 811 0 1099 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 974 1 1250 1 1487 1 
COMMENT             1616 1 1722 0 1935 0 2049 0 3383 1 4006 0 4293 0 4474 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 26 252 0 446 0 640 0 801 0 
COMMENT             807 0 893 0 920 0 1125 0 1163 0 1673 0 1802 0 2021 0 2378 0 3772 0 
COMMENT             3808 0 3825 0 4083 0 4129 0 4147 0 4488 0 4593 0 4605 0 4683 0 4691 
COMMENT             0 4702 0 4777 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 13 304 0 498 0 692 0 1255 
COMMENT             0 1380 0 1492 0 1567 0 1621 0 2277 0 2444 0 5181 0 5194 0 5315 0 0 
COMMENT             "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 23 17 0 102 0 153 0 849 0 
COMMENT             1121 0 1538 0 2098 0 2103 0 2109 0 2647 0 2732 0 2825 0 2849 0 2869 
COMMENT             0 3245 0 3356 0 3459 0 3461 0 3561 0 3893 0 4386 0 4716 0 5297 0 0 
COMMENT             "") #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 48 12 0 15 0 40 0 146 0 
COMMENT             149 0 215 0 228 0 409 0 422 0 603 0 616 0 1001 0 1008 0 1059 0 1062 
COMMENT             0 1156 0 1371 0 1430 0 1452 0 1791 0 1954 0 2103 0 2106 0 2109 0 
COMMENT             2209 0 2311 0 2327 0 2603 0 2606 0 2712 0 2737 0 2740 0 2838 0 2852 
COMMENT             0 2874 0 3314 0 3423 0 3710 0 3939 0 4034 0 4061 0 4400 0 4728 0 
COMMENT             4934 0 4937 0 5002 0 5145 0 5300 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1423 0 4033 0 4053 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 38 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 271 0 465 0 659 0 818 0 1003 0 1010 0 1333 0 
COMMENT             1366 0 1414 0 2098 0 2313 0 2322 0 2402 0 2579 0 2583 0 2647 0 2651 
COMMENT             0 2655 0 2724 0 2728 0 2732 0 2742 0 2893 0 2907 0 2950 0 3326 0 
COMMENT             4339 0 4730 0 5290 0 5327 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 13 897 0 990 0 1041 0 1317 
COMMENT             1 1581 0 1596 0 2037 1 2037 0 2307 0 2496 0 3066 0 3799 0 4200 1 0 
COMMENT             "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 26 253 0 447 0 641 0 802 0 
COMMENT             808 0 894 0 921 0 1126 0 1164 0 1674 0 1803 0 2022 0 2379 0 3773 0 
COMMENT             3809 0 3826 0 4084 0 4130 0 4148 0 4489 0 4594 0 4606 0 4684 0 4692 
COMMENT             0 4703 0 4778 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 7 1185 0 1196 0 1638 0 2071 
COMMENT             0 2821 0 3294 0 3970 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 25 241 0 266 0 435 0 460 0 
COMMENT             629 0 654 0 825 0 900 0 1128 0 1173 0 1209 0 1443 0 1473 0 1797 0 
COMMENT             2227 0 2582 0 3424 0 3508 0 3613 0 4006 0 4042 0 4120 0 4130 0 4621 
COMMENT             0 5341 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 91 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 202 0 212 0 225 0 396 0 406 0 419 0 590 0 600 0 613 0 741 0 
COMMENT             747 0 771 0 816 0 820 0 877 0 911 0 937 0 1008 0 1049 0 1182 0 1230 
COMMENT             0 1263 0 1283 0 1335 0 1368 0 1383 0 1427 0 1484 0 1584 0 1632 0 
COMMENT             1690 0 1740 0 1788 0 1940 0 1959 0 2106 0 2248 0 2338 0 2343 0 2368 
COMMENT             0 2404 0 2603 0 2631 0 2667 0 2698 0 2712 0 2737 0 2952 0 2966 0 
COMMENT             2980 0 3061 0 3165 0 3203 0 3223 0 3324 0 3328 0 3368 0 3401 0 3420 
COMMENT             0 3474 0 4061 0 4099 0 4162 0 4172 0 4262 0 4328 0 4337 0 4341 0 
COMMENT             4367 0 4408 0 4420 0 4783 0 4812 0 4855 0 4866 0 4931 0 5010 0 5035 
COMMENT             0 5040 0 5086 0 5176 0 5274 0 5300 0 5318 0 5329 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 16 32 0 84 0 197 0 391 0 
COMMENT             585 0 1078 0 1844 0 2504 0 2662 0 2714 0 3290 0 3525 0 3951 0 4491 
COMMENT             0 4657 0 5066 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 26 251 0 445 0 639 0 800 0 
COMMENT             806 0 892 0 919 0 1124 0 1162 0 1672 0 1801 0 2020 0 2377 0 3771 0 
COMMENT             3807 0 3824 0 4082 0 4128 0 4146 0 4487 0 4592 0 4604 0 4682 0 4690 
COMMENT             0 4701 0 4776 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3874 0 4566 0 4585 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 2104 0 2601 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 1658 1 1997 1 2057 1 
COMMENT             3653 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 967 1 1458 1 2311 0 
COMMENT             4310 0 5138 0 5284 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 10 934 0 1159 0 1363 
COMMENT             0 1407 1 1606 0 1654 0 1682 0 3786 0 4383 0 4798 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1156 0 1237 0 2667 0 2692 0 2693 0 2703 0 2704 0 3378 0 3413 0 
COMMENT             3914 0 4265 0 4961 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 741 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 3472 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2119 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 18 219 0 413 0 607 0 812 
COMMENT             0 990 0 1100 0 1260 0 1554 0 1581 0 1596 0 1836 0 2037 0 2116 0 
COMMENT             2371 0 2382 0 3019 0 3799 0 5243 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 25 240 0 265 0 434 0 459 0 
COMMENT             628 0 653 0 824 0 899 0 1127 0 1172 0 1208 0 1442 0 1472 0 1796 0 
COMMENT             2226 0 2581 0 3423 0 3507 0 3612 0 4005 0 4041 0 4119 0 4129 0 4620 
COMMENT             0 5340 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 9 174 1 368 1 562 1 1543 
COMMENT             0 2329 0 2845 1 2914 0 3345 0 3355 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 12 912 0 1051 0 1134 0 
COMMENT              1385 0 1653 0 2596 0 3062 0 3475 0 4091 0 4313 0 4330 0 4409 0 0 
COMMENT              "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 48 10 0 13 0 38 0 144 0 
COMMENT              147 0 213 0 226 0 407 0 420 0 601 0 614 0 999 0 1006 0 1057 0 1060 
COMMENT              0 1154 0 1369 0 1428 0 1450 0 1789 0 1952 0 2101 0 2104 0 2107 0 
COMMENT              2207 0 2309 0 2325 0 2601 0 2604 0 2710 0 2735 0 2738 0 2836 0 
COMMENT              2850 0 2872 0 3312 0 3421 0 3708 0 3937 0 4032 0 4059 0 4398 0 
COMMENT              4726 0 4932 0 4935 0 5000 0 5143 0 5298 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 11 961 1 1237 1 1474 1 
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COMMENT                              "BpmI: 3 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                              "BsmFI: 3 sites") 15 19 "GGGACNNNNNNNNNNNNNNN" 
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COMMENT                              "BspMI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                              "BsrGI: 2 sites") 1 5 "TGTACA" "ACATGT") 
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COMMENT                              "BssHII: 5 sites") 1 5 "GCGCGC" "CGCGCG") 
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COMMENT                              "MluI: 2 sites") 1 5 "ACGCGT" "TGCGCA") 
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COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
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COMMENT                              "MscI: 3 sites") 3 3 "TGGCCA" "ACCGGT") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..737
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xcore2_eHGT_779m
     misc_feature    744..796
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     CDS             824..845
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /Original_Translation="HHHHHH"
                     /vntifkey="4"
                     /label=6xHis
     source          <824..>2093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    824..2093
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=HisTag_GCaMP8s
     misc_feature    2122..2367
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2381..2584
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2607..2747
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2839..3145
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3664..4521
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4672..5339
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1302 a      1343 c      1460 g      1239 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagtt tgagacaaat taaggaaaag 
      181 cgggttggga ggagtctgag gctgtgggtg gctgcctcga gaggctgctc acaacctgac 
      241 atgtttcaga tcttttgaca cggtcatgca agcaacagaa acttatatgg tttttagtct 
      301 cccaggtagc agggaaatga tgatgtggga ggattagggg ggggaaaata agtttgagac 
      361 aaattaagga aaagcgggtt gggaggagtc tgaggctgtg ggtggctgcc tcgagaggct 
      421 gctcacaacc tgacatgttt cagatctttt gacacggtca tgcaagcaac agaaacttat 
      481 atggttttta gtctcccagg tagcagggaa atgatgatgt gggaggatta ggggggggaa 
      541 aataagtttg agacaaatta aggaaaagcg ggttgggagg agtctgaggc tgtgggtggc 
      601 tgcctcgaga ggctgctcac aacctgacat gtttcagatc ttttgacacg gtcatgcaag 
      661 caacagaaac ttatatggtt tttagtctcc caggtagcag ggaaatgatg atgtgggagg 
      721 attagggggg ggaaaatgag ctcgggctgg gcataaaagt cagggcagag ccatctattg 
      781 cttacatttg cttctgggat ccagatcttt cgaagctagc caccatgcat catcaccatc 
      841 atcacacgcg tcgcaagaag accttcaagg aggtggccac cgccgtgaag atcatcgcca 
      901 tgctgatggg cctgaagatc aacgtctata tcaaggccga caagcagaag aacggcatca 
      961 aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact 
     1021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 
     1081 gcgtggagtc caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg 
     1141 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg 
     1201 gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     1261 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg 
     1321 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     1381 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     1441 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca 
     1501 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     1561 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     1621 tggggcacaa gctggagtac aacctgccgg accaactgac tgaagagcag atcgcagaat 
     1681 acaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca accaaggagc 
     1741 tggggacggt gatgcggtct ctcggacaca accccacaga agcagagctg caggacatga 
     1801 tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatga 
     1861 tggcaagaaa aatgaaatac agggacacgg aagaagaaat tagagaagcg ttcggtgtgt 
     1921 ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc 
     1981 ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg 
     2041 gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag taaggcgcgc 
     2101 cgcggccgcg aattcgatat cataatcaac ctctggatta caaaatttgt gaaagattga 
     2161 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     2221 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     2281 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     2341 ggctgttggg cactgacaat tccgtggctc gagagatctt cgactgtgcc ttctagttgc 
     2401 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 
     2461 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 
     2521 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 
     2581 catgcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 
     2641 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 
     2701 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 
     2761 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 
     2821 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     2881 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     2941 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     3001 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 
     3061 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3121 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta 
     3181 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     3241 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 
     3301 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 
     3361 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 
     3421 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 
     3481 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 
     3541 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     3601 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     3661 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     3721 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     3781 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     3841 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     3901 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     3961 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     4021 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     4081 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     4141 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     4201 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     4261 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     4321 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     4381 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     4441 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     4501 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     4561 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     4621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     4681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     4741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     4801 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 
     4861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     4921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     4981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     5041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     5101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     5161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     5221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     5281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     5341 atgt 
//
