LOCUS       CN4361_pAAV-3xcore2_eHGT_452h-minBglobin-jGCaMP8s-WPRE3-BGHpA        5447 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...ml-el4vzfj
VERSION     urn.local...ml-el4vzfj
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN4361_pAAV-3xcore2_eHGT_452h-minBglobin-jGCaMP8s-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN4361_pAAV-3xcore2_eHGT_452h-minBglobin-jGCaMP8s-WPRE3-BGHpA" 0 
COMMENT       5447 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1277048405 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5447 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3386 0 3883 0 5129 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 41 0 52 0 844 0 2472 0 
COMMENT            2796 0 2807 0 3263 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 900 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2138 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2214 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 270 0 498 0 726 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 7 0 1895 0 2849 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2798 0 2809 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2796 0 2807 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3636 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1297 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 10 1120 0 1270 1 1318 
COMMENT             1 1351 1 1672 1 1828 1 1876 1 3312 1 3509 1 4251 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 57 38 0 143 1 147 0 378 0 
COMMENT             606 0 834 0 983 0 1080 0 1100 1 1253 0 1256 0 1295 1 1530 0 1571 0 
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COMMENT             1 2703 1 2707 0 2812 1 2862 1 2888 1 2901 0 2938 1 2952 1 2955 1 
COMMENT             2983 0 3010 0 3415 0 3441 0 3451 0 3490 0 3664 1 3711 0 3810 1 3919 
COMMENT             1 3996 1 4040 0 4161 1 4207 0 4398 1 4489 1 4851 0 4860 1 4995 0 
COMMENT             5105 1 5226 1 5245 1 5372 1 5400 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3369 0 3953 0 4326 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 7 1036 0 1465 0 1509 1 
COMMENT             1708 0 1784 0 3889 0 4901 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 32 12 0 272 0 354 0 500 0 
COMMENT             582 0 728 0 810 0 843 0 918 0 1110 0 1284 0 1332 0 1365 0 1437 0 
COMMENT             1470 0 1686 0 1734 0 1842 0 1890 0 2061 0 2840 0 3069 0 3326 0 3504 
COMMENT             0 3523 0 4202 0 4265 0 4365 0 4886 0 5143 0 5189 0 5279 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 11 895 1 908 0 1270 0 
COMMENT             3916 0 3920 1 4237 0 4700 1 4701 0 4797 1 4799 0 4885 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2199 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4380 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 1233 0 1752 0 2696 0 
COMMENT             4191 0 4413 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2856 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 972 0 2682 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 967 0 2677 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 24 21 0 341 1 569 1 797 1 
COMMENT             1119 0 1145 1 1148 1 1457 1 1563 0 1877 1 2065 0 2296 1 2414 1 2827 
COMMENT             1 2849 0 2986 0 3510 1 4121 1 4512 0 4815 1 5021 1 5024 1 5114 0 
COMMENT             5446 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 19 133 0 882 0 947 0 1002 
COMMENT             1 1147 0 1360 0 1612 0 1813 1 1956 1 2026 1 2134 1 2140 1 2516 0 
COMMENT             2718 1 3141 1 3173 0 4098 1 4385 1 4509 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 14 970 1 1071 0 1113 0 
COMMENT             1164 0 1386 0 1398 0 1446 0 1527 0 1638 0 1728 0 1944 0 2412 0 3129 
COMMENT             0 4943 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 14 971 1 1071 0 1113 0 
COMMENT             1164 0 1386 0 1398 0 1446 0 1527 0 1638 0 1728 0 1944 0 2412 0 3129 
COMMENT             0 4944 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2297 1 3718 0 5245 0 
COMMENT             0 "") 
COMMENT        #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1901 0 2120 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 11 131 0 168 0 396 0 624 0 
COMMENT             919 0 2497 0 2719 0 3003 0 4362 0 4697 0 4950 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 930 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 2 906 0 2478 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4515 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 922 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 859 0 1261 0 1756 0 
COMMENT             4486 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 7 197 1 425 1 653 1 
COMMENT             3951 0 4819 0 5060 1 5358 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2793 0 2853 0 3633 
COMMENT             0 4015 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1865 0 4489 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 19 365 0 593 0 821 0 938 
COMMENT             0 1066 0 1413 1 1687 0 1702 0 1847 1 1908 1 2603 0 2852 1 2888 0 
COMMENT             3404 1 3494 0 3857 1 4102 0 4297 1 5349 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 12 23 1 98 0 1171 1 
COMMENT             1937 1 2598 1 2756 1 2831 0 3407 0 4068 0 4585 1 4751 1 5160 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 1342 0 2353 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 849 1 1842 1 5139 0 0 
COMMENT             "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 1126 0 1440 1 1537 0 
COMMENT             2072 0 0 "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 5 1865 0 3518 0 3560 1 
COMMENT             3713 1 4489 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3518 0 3560 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 3 1836 0 1860 0 1999 0 0 
COMMENT             "") #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 12 24 1 97 0 1172 1 
COMMENT             1938 1 2599 1 2757 1 2830 0 3406 0 4067 0 4586 1 4752 1 5161 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3610 0 3715 0 4723 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1754 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2703 1 3012 0 3713 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 2043 0 4321 0 4495 1 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1286 0 2172 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 10 2269 0 3245 0 3910 0 
COMMENT             4080 1 4349 0 4392 0 4510 0 4916 0 5028 1 5041 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 2199 0 2748 
COMMENT             0 2833 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1492 1 3579 1 3886 0 
COMMENT             5270 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 913 0 1201 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 1076 1 1352 1 1589 1 
COMMENT             1718 1 1824 0 2037 0 2151 0 3486 1 4109 0 4396 0 4577 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 26 204 0 432 0 660 0 903 0 
COMMENT             909 0 995 0 1022 0 1227 0 1265 0 1775 0 1904 0 2123 0 2481 0 3875 0 
COMMENT             3911 0 3928 0 4186 0 4232 0 4250 0 4591 0 4696 0 4708 0 4786 0 4794 
COMMENT             0 4805 0 4880 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 13 382 0 610 0 838 0 1357 
COMMENT             0 1482 0 1594 0 1669 0 1723 0 2380 0 2547 0 5284 0 5297 0 5418 0 0 
COMMENT             "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 23 17 0 102 0 153 0 951 0 
COMMENT             1223 0 1640 0 2201 0 2206 0 2212 0 2750 0 2835 0 2928 0 2952 0 2972 
COMMENT             0 3348 0 3459 0 3562 0 3564 0 3664 0 3996 0 4489 0 4819 0 5400 0 0 
COMMENT             "") #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 45 12 0 15 0 40 0 146 0 
COMMENT             149 0 357 0 585 0 813 0 1103 0 1110 0 1161 0 1164 0 1258 0 1473 0 
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COMMENT             3526 0 3813 0 4042 0 4137 0 4164 0 4503 0 4831 0 5037 0 5040 0 5105 
COMMENT             0 5248 0 5403 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1525 0 4136 0 4156 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 38 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 243 0 471 0 699 0 920 0 1105 0 1112 0 1435 0 
COMMENT             1468 0 1516 0 2201 0 2416 0 2425 0 2505 0 2682 0 2686 0 2750 0 2754 
COMMENT             0 2758 0 2827 0 2831 0 2835 0 2845 0 2996 0 3010 0 3053 0 3429 0 
COMMENT             4442 0 4833 0 5393 0 5430 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 13 999 0 1092 0 1143 0 1419 
COMMENT             1 1683 0 1698 0 2139 1 2139 0 2410 0 2599 0 3169 0 3902 0 4303 1 0 
COMMENT             "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 26 205 0 433 0 661 0 904 0 
COMMENT             910 0 996 0 1023 0 1228 0 1266 0 1776 0 1905 0 2124 0 2482 0 3876 0 
COMMENT             3912 0 3929 0 4187 0 4233 0 4251 0 4592 0 4697 0 4709 0 4787 0 4795 
COMMENT             0 4806 0 4881 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 10 313 0 541 0 769 0 1287 0 
COMMENT             1298 0 1740 0 2173 0 2924 0 3397 0 4073 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 25 199 0 242 0 427 0 470 0 
COMMENT             655 0 698 0 927 0 1002 0 1230 0 1275 0 1311 0 1545 0 1575 0 1899 0 
COMMENT             2330 0 2685 0 3527 0 3611 0 3716 0 4109 0 4145 0 4223 0 4233 0 4724 
COMMENT             0 5444 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 94 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 218 0 272 0 354 0 371 0 446 0 500 0 582 0 599 0 674 0 728 0 
COMMENT             810 0 827 0 843 0 849 0 873 0 918 0 922 0 979 0 1013 0 1039 0 1110 
COMMENT             0 1151 0 1284 0 1332 0 1365 0 1385 0 1437 0 1470 0 1485 0 1529 0 
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COMMENT             4431 0 4440 0 4444 0 4470 0 4511 0 4523 0 4886 0 4915 0 4958 0 4969 
COMMENT             0 5034 0 5113 0 5138 0 5143 0 5189 0 5279 0 5377 0 5403 0 5421 0 
COMMENT             5432 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 13 32 0 84 0 1180 0 1946 0 
COMMENT             2607 0 2765 0 2817 0 3393 0 3628 0 4054 0 4594 0 4760 0 5169 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 26 203 0 431 0 659 0 902 0 
COMMENT             908 0 994 0 1021 0 1226 0 1264 0 1774 0 1903 0 2122 0 2480 0 3874 0 
COMMENT             3910 0 3927 0 4185 0 4231 0 4249 0 4590 0 4695 0 4707 0 4785 0 4793 
COMMENT             0 4804 0 4879 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3977 0 4669 0 4688 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 2207 0 2704 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 1760 1 2099 1 2159 1 
COMMENT             3756 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1069 1 1560 1 2414 0 
COMMENT             4413 0 5241 0 5387 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 11 859 0 1036 0 1261 
COMMENT             0 1465 0 1509 1 1708 0 1756 0 1784 0 3889 0 4486 0 4901 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1258 0 1339 0 2770 0 2795 0 2796 0 2806 0 2807 0 3481 0 3516 0 
COMMENT             4017 0 4368 0 5064 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 843 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 3575 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2222 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 18 205 0 433 0 661 0 914 
COMMENT             0 1092 0 1202 0 1362 0 1656 0 1683 0 1698 0 1938 0 2139 0 2219 0 
COMMENT             2474 0 2485 0 3122 0 3902 0 5346 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 25 198 0 241 0 426 0 469 0 
COMMENT             654 0 697 0 926 0 1001 0 1229 0 1274 0 1310 0 1544 0 1574 0 1898 0 
COMMENT             2329 0 2684 0 3526 0 3610 0 3715 0 4108 0 4144 0 4222 0 4232 0 4723 
COMMENT             0 5443 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 9 385 0 613 0 841 0 1645 
COMMENT             0 2432 0 2948 1 3017 0 3448 0 3458 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 12 1014 0 1153 0 1236 0 
COMMENT              1487 0 1755 0 2699 0 3165 0 3578 0 4194 0 4416 0 4433 0 4512 0 0 
COMMENT              "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 45 10 0 13 0 38 0 144 0 
COMMENT              147 0 355 0 583 0 811 0 1101 0 1108 0 1159 0 1162 0 1256 0 1471 0 
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COMMENT              5038 0 5103 0 5246 0 5401 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 11 1063 1 1339 1 1576 1 
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COMMENT                              "BfrBI: 1 site") 3 3 "ATGCAT" "TACGTA") 
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COMMENT                              "BglII: 2 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BmrI: 1 site") 11 10 "ACTGGGNNNNNN" 
COMMENT                             "TGACCCNNNNNN") 
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COMMENT                              "BmtI: 1 site") 5 1 "GCTAGC" "CGATCG") 
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COMMENT                                       #385=(CLinePar 
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COMMENT                              "BpmI: 4 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
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COMMENT                              "BsaHI: 5 sites") 2 4 "GRCGYC" "CYGCRG") 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
COMMENT                             "CCAGAGNNNNNN") 
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COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
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COMMENT                               (CObjectList)) "BseMII: 12 sites") 15 13 
COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
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COMMENT                                             " N2: 2353 (on complementary strand)" 
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COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
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COMMENT                                             " N2: 1842 (on complementary strand)" 
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COMMENT                              "BseYI: 3 sites") 1 5 "CCCAGC" "GGGTCG") 
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COMMENT                              "BsgI: 4 sites") 22 20 "GTGCAGNNNNNNNNNNNNNNNNN" 
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COMMENT                                       #420=(CLinePar 
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COMMENT                                             " N3: 3560 (on complementary strand)" 
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COMMENT                              "BsmAI: 5 sites") 6 10 "GTCTCNNNNNN" "CAGAGNNNNNN") 
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COMMENT                                             " N2: 3560 (on complementary strand)" 
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COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
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COMMENT                              "BsmFI: 3 sites") 15 19 "GGGACNNNNNNNNNNNNNNN" 
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COMMENT                               (CParagraph 104592776 (8 0) 1 1 1 0 178) 
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COMMENT                             "CTTACGNN") 
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COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
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COMMENT                             "AGGCCT") 
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COMMENT                              "BspHI: 3 sites") 1 5 "TCATGA" "AGTACT") 
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COMMENT                              "BspMI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
COMMENT                             "TGGACGNNNNNNNNN") 
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COMMENT                              "BsrBI: 3 sites") 3 3 "CCGCTC" "GGCGAG") 
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COMMENT                                       #452=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #66# 3) 1 2 2 0 
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COMMENT                                             " N3: 4495 (on complementary strand)" 
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COMMENT                             "GCAATGNNN" "CGTTACNNN") 
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COMMENT                              "BsrGI: 2 sites") 1 5 "TGTACA" "ACATGT") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594408 (8 0) 1 1 1 0 178) 
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COMMENT                             "TGACCNN") 
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COMMENT                                       #461=(CLinePar 
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COMMENT                                       #462=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #69# 3) 1 2 2 0 
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COMMENT                                       #463=(CLinePar 
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COMMENT                                              191) " N4: 2748 " 1) 
COMMENT                                       #464=(CLinePar 
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COMMENT                              "BssHII: 5 sites") 1 5 "GCGCGC" "CGCGCG") 
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COMMENT                                             " N1: 1492 (on complementary strand)" 
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COMMENT                                       #468=(CLinePar 
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COMMENT                                             " N2: 3579 (on complementary strand)" 
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COMMENT                                       #469=(CLinePar 
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COMMENT                                       #470=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #70# 4) 1 2 2 0 
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COMMENT                              "BssSI: 4 sites") 1 5 "CACGAG" "GTGCTC") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594536 (8 0) 1 1 1 0 178) 
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COMMENT                                       #473=(CLinePar 
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COMMENT                                       #474=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #71# 2) 1 2 2 0 
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COMMENT                              "BstBI: 2 sites") 2 4 "TTCGAA" "AAGCTT") 
COMMENT                       #475=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104593672 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstEII: 0 sites") 1 6 "GGTNACC" 
COMMENT                             "CCANTGG") 
COMMENT                       #476=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594152 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstF5I: 11 sites") 7 5 "GGATGNNN" 
COMMENT                             "CCTACNNN") 
COMMENT                       #477=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104593960 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstKTI: 26 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #478=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104593768 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 13 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #479=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594344 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 23 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #480=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594568 (8 0) 1 1 1 0 178) 
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COMMENT                             "CATATG") 
COMMENT                       #481=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104593704 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 45 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #482=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104593800 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #483=(CGroupPar 
COMMENT                                      (CParagraph 0 (10 #79# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #484=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 1) 1 2 2 0 
COMMENT                                              191) " N1: 1525 " 1) 
COMMENT                                       #485=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 2) 1 2 2 0 
COMMENT                                              191) " N2: 4136 " 1) 
COMMENT                                       #486=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #79# 3) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N3: 4156 (on complementary strand)" 
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COMMENT                             "GCAGTGNNN" "CGTCACNNN") 
COMMENT                       #487=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594504 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 38 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #488=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
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COMMENT                                             " N4: 3756 (on complementary strand)" 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                             "CTGRAGNNNNNNNNNNNNNNNNN" "GACYTCNNNNNNNNNNNNNNNNN") 
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COMMENT                              "EcoICRI: 1 site") 3 3 "GAGCTC" "CTCGAG") 
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COMMENT                             "CCCGCNNNNNNN" "GGGCGNNNNNNN") 
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COMMENT                              (CGroupPar 
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COMMENT                               (CObjectList)) "Fnu4HI: 45 sites") 2 3 "GCNGC" 
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COMMENT                               (CParagraph 104595016 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "FokI: 11 sites") 14 18 
COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
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COMMENT                              "FspI: 1 site") 3 3 "TGCGCA" "ACGCGT") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
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COMMENT                               (CObjectList)) "HaeIII: 25 sites") 2 2 "GGCC" 
COMMENT                             "CCGG") 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104595176 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HgaI: 7 sites") 10 15 
COMMENT                             "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
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COMMENT                               (CObjectList)) "HhaI: 31 sites") 3 1 "GCGC" 
COMMENT                             "CGCG") 
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COMMENT                                             " N2: 498 " 1) 
COMMENT                                       #547=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                             " N3: 726 " 1))))) 
COMMENT                              "HindIII: 3 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                       #554=(CLinePar 
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COMMENT                              "HinfI: 5 sites") 1 4 "GANTC" "CTNAG") 
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COMMENT                              "HpaI: 1 site") 3 3 "GTTAAC" "CAATTG") 
COMMENT                       #559=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104595592 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpaII: 24 sites") 1 3 "CCGG" 
COMMENT                             "GGCC") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594632 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HphI: 21 sites") 13 12 
COMMENT                             "GGTGANNNNNNNNN" "CCACTNNNNNNNNN") 
COMMENT                       #561=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104595496 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Hpy99I: 10 sites") 6 1 "NCGWCGN" 
COMMENT                             "NGCWGCN") 
COMMENT                       #562=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594824 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyAV: 13 sites") 11 10 
COMMENT                             "CCTTCNNNNNNN" "GGAAGNNNNNNN") 
COMMENT                       #563=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594888 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4I: 25 sites") 3 1 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #564=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104932696 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "HpyCH4IV: 14 sites") 1 3 "ACGT" 
COMMENT                             "TGCA") 
COMMENT                       #565=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104931896 (8 0) 1 1 1 0 178) 
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COMMENT                             "ACGT") 
COMMENT                       #566=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                              "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG") 
COMMENT                       #569=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594856 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
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COMMENT                                      (CParagraph 0 (10 #120# 0) 1 2 2 0 180) 
COMMENT                                      (CObjectList 
COMMENT                                       #571=(CLinePar 
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COMMENT                              "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") 
COMMENT                       #572=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104595304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MaeIII: 17 sites") 1 6 "NGTNACN" 
COMMENT                             "NCANTGN") 
COMMENT                       #573=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104595528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboI: 26 sites") 1 5 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #574=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594984 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboII: 20 sites") 13 12 
COMMENT                             "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN") 
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COMMENT                               (CParagraph 104595336 (8 0) 1 1 1 0 178) 
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COMMENT                             "GTTAAC") 
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COMMENT                               (CParagraph 104594600 (8 0) 1 1 1 0 178) 
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COMMENT                                       #579=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #125# 2) 1 2 2 0 
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COMMENT                              "MluI: 2 sites") 1 5 "ACGCGT" "TGCGCA") 
COMMENT                       #580=(CRSFolderPar 
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COMMENT                               (CParagraph 104596584 (8 0) 1 1 1 0 178) 
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COMMENT                                       #583=(CLinePar 
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COMMENT                                       #584=(CLinePar 
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COMMENT                                             " N3: 3220 (on complementary strand)" 
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COMMENT                                       #585=(CLinePar 
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COMMENT                                       #586=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #126# 5) 1 2 2 0 
COMMENT                                              191) 
COMMENT                                             " N5: 5067 (on complementary strand)" 
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COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
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COMMENT                               (CParagraph 104596136 (8 0) 1 1 1 0 178) 
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COMMENT                                             " N1: 3181 (on complementary strand)" 
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COMMENT                                             " N2: 5050 (on complementary strand)" 
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COMMENT                                       #591=(CLinePar 
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COMMENT                                              191) 
COMMENT                                             " N3: 5234 (on complementary strand)" 
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COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
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COMMENT                             (CFolderPar 
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COMMENT                               (CParagraph 104596616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MnlI: 42 sites") 11 10 
COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
COMMENT                       #593=(CRSFolderPar 
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COMMENT                                       #597=(CLinePar 
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COMMENT                              "MscI: 3 sites") 3 3 "TGGCCA" "ACCGGT") 
COMMENT                       #598=(CRSFolderPar 
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COMMENT                               (CParagraph 104596392 (8 0) 1 1 1 0 178) 
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COMMENT                             "AATT") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..839
                     /created_by="ximena.opitzaraya"
                     /modified_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xc2_eHGT_452h
     misc_feature    846..898
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     CDS             926..947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /Original_Translation="HHHHHH"
                     /vntifkey="4"
                     /label=6xHis
     source          <926..>2195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    926..2195
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=HisTag_GCaMP8s
     misc_feature    2225..2470
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2484..2687
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2710..2850
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2942..3248
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3767..4624
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4775..5442
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1356 a      1442 c      1365 g      1284 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgaa aaatacctag tcttgaactt 
      181 tggagttcca aattccacat gatcgacttc ctcaaggctt ttcacttgac gaacaaattg 
      241 catgccaaga aatcgtcaaa attctatcaa gcttaattat gcaatttctg tctataaatg 
      301 acatacctcc tgtaccctat gacacccacc tatgcactaa taagatgtga cagctgcatc 
      361 accaacaaag cctcccccgc ctggtgaaaa atacctagtc ttgaactttg gagttccaaa 
      421 ttccacatga tcgacttcct caaggctttt cacttgacga acaaattgca tgccaagaaa 
      481 tcgtcaaaat tctatcaagc ttaattatgc aatttctgtc tataaatgac atacctcctg 
      541 taccctatga cacccaccta tgcactaata agatgtgaca gctgcatcac caacaaagcc 
      601 tcccccgcct ggtgaaaaat acctagtctt gaactttgga gttccaaatt ccacatgatc 
      661 gacttcctca aggcttttca cttgacgaac aaattgcatg ccaagaaatc gtcaaaattc 
      721 tatcaagctt aattatgcaa tttctgtcta taaatgacat acctcctgta ccctatgaca 
      781 cccacctatg cactaataag atgtgacagc tgcatcacca acaaagcctc ccccgcctgg 
      841 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg 
      901 atccagatct ttcgaagcta gccaccatgc atcatcacca tcatcacacg cgtcgcaaga 
      961 agaccttcaa ggaggtggcc accgccgtga agatcatcgc catgctgatg ggcctgaaga 
     1021 tcaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac ttccacatcc 
     1081 gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca 
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtggag tccaaacttt 
     1201 cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 
     1261 ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca 
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 
     1381 acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga 
     1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 
     1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 
     1561 tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg 
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 
     1741 acaacctgcc ggaccaactg actgaagagc agatcgcaga atacaaagag gctttctccc 
     1801 tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg gtgatgcggt 
     1861 ctctcggaca caaccccaca gaagcagagc tgcaggacat gatcaatgaa gtagatgccg 
     1921 acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga aaaatgaaat 
     1981 acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag gatggcaatg 
     2041 gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag aagttaacag 
     2101 atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt caggtaaact 
     2161 acgaagagtt tgtacaaatg atgacagcga agtaagggcg cgccgcggcc gcgaattcga 
     2221 tatcataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 
     2281 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 
     2341 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg 
     2401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 
     2461 aattccgtgg ctcgagagat cttcgactgt gccttctagt tgccagccat ctgttgtttg 
     2521 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
     2581 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
     2641 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgcac gtgcggaccg 
     2701 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc 
     2761 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag 
     2821 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc 
     2881 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 
     2941 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     3001 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     3061 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     3121 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     3181 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     3241 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 
     3301 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     3361 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 
     3421 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 
     3481 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 
     3541 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 
     3601 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 
     3661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     3721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     4621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     4681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     4741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     4801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     4861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     4921 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 
     4981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     5041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     5101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     5161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     5221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     5281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     5341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     5401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt 
//
