LOCUS       CN4827_pAAV-3xcore2_eHGT_441h-minBG-HisTag_GCaMP8s-WPRE3-BGHpA        5365 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...1d-fx733k3
VERSION     urn.local...1d-fx733k3
SOURCE      
  ORGANISM  .
COMMENT     
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN4827_pAAV-3xcore2_eHGT_441h-minBG-HisTag_GCaMP8s-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN4827_pAAV-3xcore2_eHGT_441h-minBG-HisTag_GCaMP8s-WPRE3-BGHpA" 
COMMENT       0 5365 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952538996 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5365 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3304 0 3801 0 5047 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 41 0 52 0 757 0 2384 0 
COMMENT            2714 0 2725 0 3181 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 226 0 425 0 624 0 813 0 
COMMENT            0 "") #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2051 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2126 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 7 0 1808 0 2767 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2716 0 2727 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2714 0 2725 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3554 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1210 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 16 272 1 279 1 471 1 
COMMENT             478 1 670 1 677 1 1033 0 1183 1 1231 1 1264 1 1585 1 1741 1 1789 1 
COMMENT             3230 1 3427 1 4169 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 57 38 0 143 1 147 0 180 0 
COMMENT             379 0 578 0 896 0 993 0 1013 1 1166 0 1169 0 1208 1 1443 0 1484 0 
COMMENT             1551 0 1590 0 1770 1 2116 0 2118 1 2122 0 2311 1 2324 0 2337 0 2612 
COMMENT             1 2621 1 2625 0 2730 1 2780 1 2806 1 2819 0 2856 1 2870 1 2873 1 
COMMENT             2901 0 2928 0 3333 0 3359 0 3369 0 3408 0 3582 1 3629 0 3728 1 3837 
COMMENT             1 3914 1 3958 0 4079 1 4125 0 4316 1 4407 1 4769 0 4778 1 4913 0 
COMMENT             5023 1 5144 1 5163 1 5290 1 5318 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 3287 0 3871 0 4244 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 7 949 0 1378 0 1422 1 
COMMENT             1621 0 1697 0 3807 0 4819 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 35 12 0 286 0 293 0 315 0 
COMMENT             485 0 492 0 514 0 684 0 691 0 713 0 756 0 831 0 1023 0 1197 0 1245 
COMMENT             0 1278 0 1350 0 1383 0 1599 0 1647 0 1755 0 1803 0 1974 0 2758 0 
COMMENT             2987 0 3244 0 3422 0 3441 0 4120 0 4183 0 4283 0 4804 0 5061 0 5107 
COMMENT             0 5197 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 17 221 1 234 0 420 1 433 
COMMENT             0 619 1 632 0 808 1 821 0 1183 0 3834 0 3838 1 4155 0 4618 1 4619 0 
COMMENT             4715 1 4717 0 4803 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2111 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4298 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 1146 0 1665 0 2614 0 
COMMENT             4109 0 4331 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2774 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 5 329 1 528 1 727 1 885 
COMMENT             0 2594 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 5 334 1 533 1 732 1 880 0 
COMMENT             2589 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 27 21 0 273 1 280 1 472 1 
COMMENT             479 1 671 1 678 1 1032 0 1058 1 1061 1 1370 1 1476 0 1790 1 1978 0 
COMMENT             2208 1 2326 1 2745 1 2767 0 2904 0 3428 1 4039 1 4430 0 4733 1 4939 
COMMENT             1 4942 1 5032 0 5364 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 22 133 0 341 0 540 0 739 
COMMENT             0 795 0 860 0 915 1 1060 0 1273 0 1525 0 1726 1 1869 1 1939 1 2047 
COMMENT             1 2053 1 2428 0 2636 1 3059 1 3091 0 4016 1 4303 1 4427 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 14 883 1 984 0 1026 0 
COMMENT             1077 0 1299 0 1311 0 1359 0 1440 0 1551 0 1641 0 1857 0 2324 0 3047 
COMMENT             0 4861 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 14 884 1 984 0 1026 0 
COMMENT             1077 0 1299 0 1311 0 1359 0 1440 0 1551 0 1641 0 1857 0 2324 0 3047 
COMMENT             0 4862 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 3 2209 1 3636 0 5163 0 
COMMENT             0 "") 
COMMENT        #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1814 0 2033 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 8 131 0 832 0 2409 0 2637 0 
COMMENT             2921 0 4280 0 4615 0 4868 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 843 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 3 819 0 2390 0 2601 0 0 
COMMENT             "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 3 311 0 510 0 709 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4433 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 835 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1174 0 1669 0 4404 0 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 7 231 0 430 0 629 0 
COMMENT             3869 0 4737 0 4978 1 5276 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2711 0 2771 0 3551 
COMMENT             0 3933 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1778 0 4407 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 851 0 979 0 1326 1 
COMMENT             1600 0 1615 0 1760 1 1821 1 2515 0 2770 1 2806 0 3322 1 3412 0 3775 
COMMENT             1 4020 0 4215 1 5267 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 16 23 1 98 0 302 1 501 
COMMENT             1 700 1 743 1 1084 1 1850 1 2510 1 2674 1 2749 0 3325 0 3986 0 4503 
COMMENT             1 4669 1 5078 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 1255 0 2265 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 3 762 1 1755 1 5057 0 0 
COMMENT             "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 1039 0 1353 1 1450 0 
COMMENT             1985 0 0 "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 5 1778 0 3436 0 3478 1 
COMMENT             3631 1 4407 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3436 0 3478 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 4 140 1 1749 0 1773 0 
COMMENT             1912 0 0 "") #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 16 24 1 97 0 303 1 502 
COMMENT             1 701 1 744 1 1085 1 1851 1 2511 1 2675 1 2748 0 3324 0 3985 0 4504 
COMMENT             1 4670 1 5079 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3528 0 3633 0 4641 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1667 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2621 1 2930 0 3631 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 1956 0 4239 0 4413 1 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 2 1199 0 2085 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 10 2181 0 3163 0 3828 0 
COMMENT             3998 1 4267 0 4310 0 4428 0 4834 0 4946 1 4959 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 2111 0 2666 
COMMENT             0 2751 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1405 1 3497 1 3804 0 
COMMENT             5188 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 2 826 0 1114 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 989 1 1265 1 1502 1 
COMMENT             1631 1 1737 0 1950 0 2064 0 3404 1 4027 0 4314 0 4495 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 27 229 0 428 0 627 0 816 0 
COMMENT             822 0 908 0 935 0 1140 0 1178 0 1688 0 1817 0 2036 0 2393 0 2604 0 
COMMENT             3793 0 3829 0 3846 0 4104 0 4150 0 4168 0 4509 0 4614 0 4626 0 4704 
COMMENT             0 4712 0 4723 0 4798 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 16 176 0 231 0 375 0 430 0 
COMMENT             574 0 629 0 1270 0 1395 0 1507 0 1582 0 1636 0 2292 0 2459 0 5202 0 
COMMENT             5215 0 5336 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 26 17 0 102 0 153 0 221 0 
COMMENT             420 0 619 0 864 0 1136 0 1553 0 2113 0 2118 0 2124 0 2668 0 2753 0 
COMMENT             2846 0 2870 0 2890 0 3266 0 3377 0 3480 0 3482 0 3582 0 3914 0 4407 
COMMENT             0 4737 0 5318 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 51 12 0 15 0 40 0 146 0 
COMMENT             149 0 182 0 289 0 296 0 381 0 488 0 495 0 580 0 687 0 694 0 1016 0 
COMMENT             1023 0 1074 0 1077 0 1171 0 1386 0 1445 0 1467 0 1806 0 1969 0 2118 
COMMENT             0 2121 0 2124 0 2224 0 2326 0 2342 0 2624 0 2627 0 2733 0 2758 0 
COMMENT             2761 0 2859 0 2873 0 2895 0 3335 0 3444 0 3731 0 3960 0 4055 0 4082 
COMMENT             0 4421 0 4749 0 4955 0 4958 0 5023 0 5166 0 5321 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1438 0 4054 0 4074 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 37 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 291 0 490 0 689 0 833 0 1018 0 1025 0 1348 0 
COMMENT             1381 0 1429 0 2113 0 2328 0 2337 0 2417 0 2594 0 2668 0 2672 0 2676 
COMMENT             0 2745 0 2749 0 2753 0 2763 0 2914 0 2928 0 2971 0 3347 0 4360 0 
COMMENT             4751 0 5311 0 5348 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 13 912 0 1005 0 1056 0 1332 
COMMENT             1 1596 0 1611 0 2052 1 2052 0 2322 0 2511 0 3087 0 3820 0 4221 1 0 
COMMENT             "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 27 230 0 429 0 628 0 817 0 
COMMENT             823 0 909 0 936 0 1141 0 1179 0 1689 0 1818 0 2037 0 2394 0 2605 0 
COMMENT             3794 0 3830 0 3847 0 4105 0 4151 0 4169 0 4510 0 4615 0 4627 0 4705 
COMMENT             0 4713 0 4724 0 4799 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 7 1200 0 1211 0 1653 0 2086 
COMMENT             0 2842 0 3315 0 3991 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 19 840 0 915 0 1143 0 1188 
COMMENT             0 1224 0 1458 0 1488 0 1812 0 2242 0 2597 0 3445 0 3529 0 3634 0 
COMMENT             4027 0 4063 0 4141 0 4151 0 4642 0 5362 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 97 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 165 0 179 0 286 0 293 0 315 0 364 0 378 0 485 0 492 0 514 0 
COMMENT             563 0 577 0 684 0 691 0 713 0 756 0 762 0 786 0 831 0 835 0 892 0 
COMMENT             926 0 952 0 1023 0 1064 0 1197 0 1245 0 1278 0 1298 0 1350 0 1383 0 
COMMENT             1398 0 1442 0 1499 0 1599 0 1647 0 1705 0 1755 0 1803 0 1955 0 1974 
COMMENT             0 2121 0 2263 0 2353 0 2358 0 2383 0 2419 0 2624 0 2652 0 2688 0 
COMMENT             2719 0 2733 0 2758 0 2973 0 2987 0 3001 0 3082 0 3186 0 3224 0 3244 
COMMENT             0 3345 0 3349 0 3389 0 3422 0 3441 0 3495 0 4082 0 4120 0 4183 0 
COMMENT             4193 0 4283 0 4349 0 4358 0 4362 0 4388 0 4429 0 4441 0 4804 0 4833 
COMMENT             0 4876 0 4887 0 4952 0 5031 0 5056 0 5061 0 5107 0 5197 0 5295 0 
COMMENT             5321 0 5339 0 5350 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 17 32 0 84 0 311 0 510 0 
COMMENT             709 0 752 0 1093 0 1859 0 2519 0 2683 0 2735 0 3311 0 3546 0 3972 0 
COMMENT             4512 0 4678 0 5087 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 27 228 0 427 0 626 0 815 0 
COMMENT             821 0 907 0 934 0 1139 0 1177 0 1687 0 1816 0 2035 0 2392 0 2603 0 
COMMENT             3792 0 3828 0 3845 0 4103 0 4149 0 4167 0 4508 0 4613 0 4625 0 4703 
COMMENT             0 4711 0 4722 0 4797 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3895 0 4587 0 4606 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 2119 0 2622 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 1673 1 2012 1 2072 1 
COMMENT             3674 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 982 1 1473 1 2326 0 
COMMENT             4331 0 5159 0 5305 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 10 949 0 1174 0 1378 
COMMENT             0 1422 1 1621 0 1669 0 1697 0 3807 0 4404 0 4819 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1171 0 1252 0 2688 0 2713 0 2714 0 2724 0 2725 0 3399 0 3434 0 
COMMENT             3935 0 4286 0 4982 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 756 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 3493 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2134 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 15 827 0 1005 0 1115 0 
COMMENT             1275 0 1569 0 1596 0 1611 0 1851 0 2052 0 2131 0 2386 0 2397 0 3040 
COMMENT             0 3820 0 5264 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 19 839 0 914 0 1142 0 1187 0 
COMMENT             1223 0 1457 0 1487 0 1811 0 2241 0 2596 0 3444 0 3528 0 3633 0 4026 
COMMENT             0 4062 0 4140 0 4150 0 4641 0 5361 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 6 1558 0 2344 0 2866 1 
COMMENT             2935 0 3366 0 3376 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 12 927 0 1066 0 1149 0 
COMMENT              1400 0 1668 0 2617 0 3083 0 3496 0 4112 0 4334 0 4351 0 4430 0 0 
COMMENT              "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 51 10 0 13 0 38 0 144 0 
COMMENT              147 0 180 0 287 0 294 0 379 0 486 0 493 0 578 0 685 0 692 0 1014 0 
COMMENT              1021 0 1072 0 1075 0 1169 0 1384 0 1443 0 1465 0 1804 0 1967 0 
COMMENT              2116 0 2119 0 2122 0 2222 0 2324 0 2340 0 2622 0 2625 0 2731 0 
COMMENT              2756 0 2759 0 2857 0 2871 0 2893 0 3333 0 3442 0 3729 0 3958 0 
COMMENT              4053 0 4080 0 4419 0 4747 0 4953 0 4956 0 5021 0 5164 0 5319 0 0 
COMMENT              "") 
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COMMENT                              "BlpI: 3 sites") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                              "BmrI: 1 site") 11 10 "ACTGGGNNNNNN" 
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COMMENT                              "BpmI: 3 sites") 22 20 "CTGGAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsaI: 2 sites") 7 11 "GGTCTCNNNNNN" 
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COMMENT                              "BsgI: 4 sites") 22 20 "GTGCAGNNNNNNNNNNNNNNNNN" 
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COMMENT                              "BsmAI: 5 sites") 6 10 "GTCTCNNNNNN" "CAGAGNNNNNN") 
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COMMENT                              "BsmFI: 4 sites") 15 19 "GGGACNNNNNNNNNNNNNNN" 
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COMMENT                              "BspMI: 1 site") 10 14 "ACCTGCNNNNNNNNN" 
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COMMENT                              "BsrGI: 2 sites") 1 5 "TGTACA" "ACATGT") 
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COMMENT                              "BssHII: 5 sites") 1 5 "GCGCGC" "CGCGCG") 
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COMMENT                                             " N1: 1405 (on complementary strand)" 
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COMMENT                                             " N2: 3497 (on complementary strand)" 
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COMMENT                              "BssSI: 4 sites") 1 5 "CACGAG" "GTGCTC") 
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COMMENT                              "BstBI: 2 sites") 2 4 "TTCGAA" "AAGCTT") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..752
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xcore2_eHGT_441h
     misc_feature    759..811
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     CDS             839..860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /Original_Translation="HHHHHH"
                     /vntifkey="4"
                     /label=6xHis
     source          <839..>2108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    839..2108
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=HisTag_GCaMP8s
     misc_feature    2137..2382
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    complement(2250..2269)
                     /vntifkey="21"
                     /label=WPRE3-R\(JT)
     misc_feature    2396..2599
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2628..2768
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2860..3166
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3685..4542
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4693..5360
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1301 a      1434 c      1371 g      1259 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtcccac acagccccca ccacctggcc 
      181 gcccacctga aaaacttttc cccaagttct tgagaagtcg cgtaggatcc aggagagaaa 
      241 aatcaaagta ataaaacctc atttaattcc taacggaggg cagagctgcg agctgcaatt 
      301 atcttaatgc tgagctattt ttactctctc tccatctctg tcttctgcct ttctcccaca 
      361 cagcccccac cacctggccg cccacctgaa aaacttttcc ccaagttctt gagaagtcgc 
      421 gtaggatcca ggagagaaaa atcaaagtaa taaaacctca tttaattcct aacggagggc 
      481 agagctgcga gctgcaatta tcttaatgct gagctatttt tactctctct ccatctctgt 
      541 cttctgcctt tctcccacac agcccccacc acctggccgc ccacctgaaa aacttttccc 
      601 caagttcttg agaagtcgcg taggatccag gagagaaaaa tcaaagtaat aaaacctcat 
      661 ttaattccta acggagggca gagctgcgag ctgcaattat cttaatgctg agctattttt 
      721 actctctctc catctctgtc ttctgccttt ctgagctcgg gctgggcata aaagtcaggg 
      781 cagagccatc tattgcttac atttgcttct gggatccaga tctttcgaag ctagccacca 
      841 tgcatcatca ccatcatcac acgcgtcgca agaagacctt caaggaggtg gccaccgccg 
      901 tgaagatcat cgccatgctg atgggcctga agatcaacgt ctatatcaag gccgacaagc 
      961 agaagaacgg catcaaggcg aacttccaca tccgccacaa catcgaggac ggcggcgtgc 
     1021 agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     1081 acaaccacta cctgagcgtg gagtccaaac tttcgaaaga ccccaacgag aagcgcgatc 
     1141 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     1201 acaagggcgg taccggaggg agcatggtga gcaagggcga ggagctgttc accggggtgg 
     1261 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     1321 agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     1381 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     1441 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     1501 acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     1561 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     1621 aggacggcaa catcctgggg cacaagctgg agtacaacct gccggaccaa ctgactgaag 
     1681 agcagatcgc agaatacaaa gaggctttct ccctatttga caaggacggg gatgggacaa 
     1741 taacaaccaa ggagctgggg acggtgatgc ggtctctcgg acacaacccc acagaagcag 
     1801 agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc gacttccctg 
     1861 agttcctgac aatgatggca agaaaaatga aatacaggga cacggaagaa gaaattagag 
     1921 aagcgttcgg tgtgtttgat aaggatggca atggctacat cagtgcagca gagcttcgcc 
     1981 acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa atgatcaggg 
     2041 aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa atgatgacag 
     2101 cgaagtaagg cgcgccgcgg ccgcgaattc gatatcataa tcaacctctg gattacaaaa 
     2161 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     2221 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     2281 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct 
     2341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggctcgagag atcttcgact 
     2401 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 
     2461 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 
     2521 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 
     2581 gaagacaata gcaggcatga gatctcacgt gcggaccgag cggccgcagg aacccctagt 
     2641 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 
     2701 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg 
     2761 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 
     2821 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     2881 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     2941 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     3001 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 
     3061 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     3121 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     3181 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     3241 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta 
     3301 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 
     3361 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 
     3421 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 
     3481 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 
     3541 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     3601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     3661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     3721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     3781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     3841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     3901 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     3961 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     4021 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     4081 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     4141 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     4201 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
     4261 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     4321 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     4381 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     4441 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     4501 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     4561 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     4621 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     4681 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     4741 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     4801 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     4861 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     4921 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     4981 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     5041 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     5101 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     5161 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     5221 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     5281 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     5341 ttttgctggc cttttgctca catgt 
//
