LOCUS       CN4871_pAAV-3xcore2_eHGT_779m-minBglobin-RiboL1-jGCaMP8s-WPRE3-BGHpA        6061 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  .
ACCESSION   urn.local...19q-g66sbt3
VERSION     urn.local...19q-g66sbt3
SOURCE      
  ORGANISM  .
COMMENT     
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN4871_pAAV-3xcore2_eHGT_779m-minBglobin-RiboL1-jGCaMP8s-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc 
COMMENT       "CN4871_pAAV-3xcore2_eHGT_779m-minBglobin-RiboL1-jGCaMP8s-WPRE3-BGHpA" 0 
COMMENT       6061 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1277048405 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 816 833 1 6061 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 4000 0 4497 0 5743 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 11 41 0 52 0 217 0 411 0 
COMMENT            605 0 742 0 2304 0 3086 0 3410 0 3421 0 3877 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 798 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2024 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2828 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 2783 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 7 0 1781 0 2261 0 3463 0 
COMMENT            0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 3412 0 3423 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 3410 0 3421 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 1 2537 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 4250 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1183 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 1 2696 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 11 1006 0 1156 1 1204 
COMMENT             1 1237 1 1558 1 1714 1 1762 1 2242 1 3926 1 4123 1 4865 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 67 38 0 143 1 147 0 181 1 
COMMENT             375 1 569 1 806 0 869 0 966 0 986 1 1139 0 1142 0 1181 1 1416 0 
COMMENT             1457 0 1524 0 1563 0 1743 1 2087 1 2114 1 2123 1 2141 1 2144 1 2194 
COMMENT             1 2199 1 2232 0 2760 1 2818 0 2820 1 2824 0 3013 1 3026 0 3039 0 
COMMENT             3308 1 3317 1 3321 0 3426 1 3476 1 3502 1 3515 0 3552 1 3566 1 3569 
COMMENT             1 3597 0 3624 0 4029 0 4055 0 4065 0 4104 0 4278 1 4325 0 4424 1 
COMMENT             4533 1 4610 1 4654 0 4775 1 4821 0 5012 1 5103 1 5465 0 5474 1 5609 
COMMENT             0 5719 1 5840 1 5859 1 5986 1 6014 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 4 2298 0 3983 0 4567 0 
COMMENT             4940 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 8 922 0 1351 0 1395 1 
COMMENT             1594 0 1670 0 2290 0 4503 0 5515 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 2737 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 28 12 0 741 0 996 0 1170 0 
COMMENT             1218 0 1251 0 1323 0 1356 0 1572 0 1620 0 1728 0 1776 0 1947 0 2256 
COMMENT             0 2463 0 2691 0 3454 0 3683 0 3940 0 4118 0 4137 0 4816 0 4879 0 
COMMENT             4979 0 5500 0 5757 0 5803 0 5893 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 12 793 1 806 0 1156 0 
COMMENT             2508 1 4530 0 4534 1 4851 0 5314 1 5315 0 5411 1 5413 0 5499 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2533 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2813 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4994 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 6 1119 0 1638 0 2364 0 
COMMENT             3310 0 4805 0 5027 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 3470 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 858 0 3296 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 853 0 3291 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 31 21 0 199 1 212 1 393 1 
COMMENT             406 1 587 1 600 1 1005 0 1031 1 1034 1 1343 1 1449 0 1763 1 1951 0 
COMMENT             2096 0 2174 0 2243 1 2382 0 2910 1 3028 1 3441 1 3463 0 3600 0 4124 
COMMENT             1 4735 1 5126 0 5429 1 5635 1 5638 1 5728 0 6060 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 19 133 0 780 0 833 0 888 
COMMENT             1 1033 0 1246 0 1498 0 1699 1 1842 1 1912 1 2020 1 2026 1 3130 0 
COMMENT             3332 1 3755 1 3787 0 4712 1 4999 1 5123 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 16 856 1 957 0 999 0 
COMMENT             1050 0 1272 0 1284 0 1332 0 1413 0 1524 0 1614 0 1830 0 2377 1 2638 
COMMENT             1 3026 0 3743 0 5557 1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 16 857 1 957 0 999 0 
COMMENT             1050 0 1272 0 1284 0 1332 0 1413 0 1524 0 1614 0 1830 0 2378 1 2639 
COMMENT             1 3026 0 3743 0 5558 1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 4 2533 0 2911 1 4332 0 
COMMENT             5859 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 1787 0 2006 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 8 131 0 2692 0 3111 0 3333 0 
COMMENT             3617 0 4976 0 5311 0 5564 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 1 816 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 4 249 0 443 0 637 0 3092 
COMMENT             0 0 "") #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 2 2114 0 5129 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 5 1147 0 1642 0 2117 0 
COMMENT             2263 0 5100 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 6 2305 1 2410 1 4565 0 
COMMENT             5433 0 5674 1 5972 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 3407 0 3467 0 4247 
COMMENT             0 4629 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 1751 0 5103 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 17 824 0 952 0 1299 1 
COMMENT             1573 0 1588 0 1733 1 1794 1 2472 1 3217 0 3466 1 3502 0 4018 1 4108 
COMMENT             0 4471 1 4716 0 4911 1 5963 1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 17 23 1 98 0 188 1 382 
COMMENT             1 576 1 1057 1 1823 1 2299 0 2486 1 3212 1 3370 1 3445 0 4021 0 
COMMENT             4682 0 5199 1 5365 1 5774 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 5 205 0 399 0 593 0 
COMMENT             1228 0 2967 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 4 747 1 1728 1 2795 0 
COMMENT             5753 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 5 1012 0 1326 1 1423 0 
COMMENT             1958 0 2194 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 14 157 1 303 0 351 1 497 
COMMENT             0 545 1 691 0 1751 0 2175 0 2239 1 2504 0 4132 0 4174 1 4327 1 5103 
COMMENT             0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 3 2239 1 4132 0 4174 1 0 
COMMENT             "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 6 1722 0 1746 0 1885 0 
COMMENT             2115 0 2142 0 2377 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 17 24 1 97 0 189 1 383 
COMMENT             1 577 1 1058 1 1824 1 2298 0 2487 1 3213 1 3371 1 3444 0 4020 0 
COMMENT             4681 0 5200 1 5366 1 5775 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 4224 0 4329 0 5337 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 1 1640 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 3317 1 3626 0 4327 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 1929 0 4935 0 5109 1 0 
COMMENT             "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 3 1172 0 2058 0 2768 0 0 
COMMENT             "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 12 2109 0 2752 0 2883 0 
COMMENT             3859 0 4524 0 4694 1 4963 0 5006 0 5124 0 5530 0 5642 1 5655 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 2813 0 3362 
COMMENT             0 3447 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1378 1 4193 1 4500 0 
COMMENT             5884 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 1 1087 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 12 962 1 1238 1 1475 1 
COMMENT             1604 1 1710 0 1923 0 2037 0 2607 0 4100 1 4723 0 5010 0 5191 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 27 252 0 446 0 640 0 801 0 
COMMENT             881 0 908 0 1113 0 1151 0 1661 0 1790 0 2009 0 2264 0 2516 0 3095 0 
COMMENT             4489 0 4525 0 4542 0 4800 0 4846 0 4864 0 5205 0 5310 0 5322 0 5400 
COMMENT             0 5408 0 5419 0 5494 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 15 304 0 498 0 692 0 1243 
COMMENT             0 1368 0 1480 0 1555 0 1609 0 2527 0 2534 0 2994 0 3161 0 5898 0 
COMMENT             5911 0 6032 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 24 17 0 102 0 153 0 837 0 
COMMENT             1109 0 1526 0 2177 0 2815 0 2820 0 2826 0 3364 0 3449 0 3542 0 3566 
COMMENT             0 3586 0 3962 0 4073 0 4176 0 4178 0 4278 0 4610 0 5103 0 5433 0 
COMMENT             6014 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 54 12 0 15 0 40 0 146 0 
COMMENT             149 0 215 0 228 0 409 0 422 0 603 0 616 0 989 0 996 0 1047 0 1050 0 
COMMENT             1144 0 1359 0 1418 0 1440 0 1779 0 1942 0 2087 0 2144 0 2165 0 2234 
COMMENT             0 2259 0 2373 0 2820 0 2823 0 2826 0 2926 0 3028 0 3044 0 3320 0 
COMMENT             3323 0 3429 0 3454 0 3457 0 3555 0 3569 0 3591 0 4031 0 4140 0 4427 
COMMENT             0 4656 0 4751 0 4778 0 5117 0 5445 0 5651 0 5654 0 5719 0 5862 0 
COMMENT             6017 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1411 0 4750 0 4770 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 42 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 271 0 465 0 659 0 991 0 998 0 1321 0 1354 0 1402 
COMMENT             0 2227 0 2465 0 2550 0 2762 0 2791 0 2815 0 3030 0 3039 0 3119 0 
COMMENT             3296 0 3300 0 3364 0 3368 0 3372 0 3441 0 3445 0 3449 0 3459 0 3610 
COMMENT             0 3624 0 3667 0 4043 0 5056 0 5447 0 6007 0 6044 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 16 885 0 978 0 1029 0 1305 
COMMENT             1 1569 0 1584 0 2025 0 2025 1 2418 0 2478 1 2682 1 3024 0 3213 0 
COMMENT             3783 0 4516 0 4917 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 27 253 0 447 0 641 0 802 0 
COMMENT             882 0 909 0 1114 0 1152 0 1662 0 1791 0 2010 0 2265 0 2517 0 3096 0 
COMMENT             4490 0 4526 0 4543 0 4801 0 4847 0 4865 0 5206 0 5311 0 5323 0 5401 
COMMENT             0 5409 0 5420 0 5495 0 0 "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 10 1173 0 1184 0 1626 0 
COMMENT             2059 0 2187 0 2475 0 2769 0 3538 0 4011 0 4687 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 28 241 0 266 0 435 0 460 0 
COMMENT             629 0 654 0 813 0 888 0 1116 0 1161 0 1197 0 1431 0 1461 0 1785 0 
COMMENT             2409 0 2586 0 2784 0 2944 0 3299 0 4141 0 4225 0 4330 0 4723 0 4759 
COMMENT             0 4837 0 4847 0 5338 0 6058 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 107 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 202 0 212 0 225 0 396 0 406 0 419 0 590 0 600 0 613 0 741 0 
COMMENT             747 0 771 0 865 0 899 0 925 0 996 0 1037 0 1170 0 1218 0 1251 0 
COMMENT             1271 0 1323 0 1356 0 1371 0 1415 0 1472 0 1572 0 1620 0 1678 0 1728 
COMMENT             0 1776 0 1928 0 1947 0 2256 0 2267 0 2319 0 2386 0 2392 0 2463 0 
COMMENT             2467 0 2491 0 2531 0 2537 0 2548 0 2593 0 2653 0 2666 0 2691 0 2713 
COMMENT             0 2764 0 2793 0 2823 0 2965 0 3055 0 3060 0 3085 0 3121 0 3320 0 
COMMENT             3348 0 3384 0 3415 0 3429 0 3454 0 3669 0 3683 0 3697 0 3778 0 3882 
COMMENT             0 3920 0 3940 0 4041 0 4045 0 4085 0 4118 0 4137 0 4191 0 4778 0 
COMMENT             4816 0 4879 0 4889 0 4979 0 5045 0 5054 0 5058 0 5084 0 5125 0 5137 
COMMENT             0 5500 0 5529 0 5572 0 5583 0 5648 0 5727 0 5752 0 5757 0 5803 0 
COMMENT             5893 0 5991 0 6017 0 6035 0 6046 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 19 32 0 84 0 197 0 391 0 
COMMENT             585 0 1066 0 1832 0 2285 0 2468 0 2495 0 3221 0 3379 0 3431 0 4007 
COMMENT             0 4242 0 4668 0 5208 0 5374 0 5783 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 27 251 0 445 0 639 0 800 0 
COMMENT             880 0 907 0 1112 0 1150 0 1660 0 1789 0 2008 0 2263 0 2515 0 3094 0 
COMMENT             4488 0 4524 0 4541 0 4799 0 4845 0 4863 0 5204 0 5309 0 5321 0 5399 
COMMENT             0 5407 0 5418 0 5493 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 4591 0 5283 0 5302 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 3 144 0 2821 0 3318 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 4 1646 1 1985 1 2045 1 
COMMENT             4370 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 955 1 1446 1 3028 0 
COMMENT             5027 0 5855 0 6001 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 13 922 0 1147 0 1351 
COMMENT             0 1395 1 1594 0 1642 0 1670 0 2117 0 2263 0 2290 0 4503 0 5100 0 
COMMENT             5515 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1144 0 1225 0 3384 0 3409 0 3410 0 3420 0 3421 0 4095 0 4130 0 
COMMENT             4631 0 4982 0 5678 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 741 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 4189 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 2400 0 2836 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 19 219 0 413 0 607 0 978 
COMMENT             0 1088 0 1248 0 1542 0 1569 0 1584 0 1824 0 2025 0 2418 0 2615 0 
COMMENT             2833 0 3088 0 3099 0 3736 0 4516 0 5960 0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 28 240 0 265 0 434 0 459 0 
COMMENT             628 0 653 0 812 0 887 0 1115 0 1160 0 1196 0 1430 0 1460 0 1784 0 
COMMENT             2408 0 2585 0 2783 0 2943 0 3298 0 4140 0 4224 0 4329 0 4722 0 4758 
COMMENT             0 4836 0 4846 0 5337 0 6057 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 12 174 1 368 1 562 1 813 
COMMENT             0 1531 0 2192 1 2753 1 3046 0 3562 1 3631 0 4062 0 4072 0 0 "") 
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COMMENT                                             " N4: 4370 (on complementary strand)" 
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COMMENT                             "CTCTTCNNNNN" "GAGAAGNNNNN") 
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COMMENT                             "GGCGGANNNNNNNNNNNN" "CCGCCTNNNNNNNNNNNN") 
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COMMENT                              "EcoRV: 2 sites") 3 3 "GATATC" "CTATAG") 
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COMMENT                             "CCCGCNNNNNNN" "GGGCGNNNNNNN") 
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COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
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COMMENT                             "GACGCNNNNNNNNNNN" "CTGCGNNNNNNNNNNN") 
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COMMENT                              "HpaI: 1 site") 3 3 "GTTAAC" "CAATTG") 
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COMMENT                               (CObjectList)) "HphI: 17 sites") 13 12 
COMMENT                             "GGTGANNNNNNNNN" "CCACTNNNNNNNNN") 
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COMMENT                               (CParagraph 104595496 (8 0) 1 1 1 0 178) 
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COMMENT                             "GTAC") 
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COMMENT                              "KasI: 1 site") 1 5 "GGCGCC" "CCGCGG") 
COMMENT                       #556=(CRSFolderPar 
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COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594856 (8 0) 1 1 1 0 178) 
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COMMENT                              "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") 
COMMENT                       #559=(CRSFolderPar 
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COMMENT                               (CParagraph 104595304 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MaeIII: 14 sites") 1 6 "NGTNACN" 
COMMENT                             "NCANTGN") 
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COMMENT                               (CParagraph 104595528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MboI: 27 sites") 1 5 "NGATCN" 
COMMENT                             "NCTAGN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar 
COMMENT                               (CParagraph 104594984 (8 0) 1 1 1 0 178) 
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COMMENT                             "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN") 
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COMMENT                             "GTTAAC") 
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COMMENT                              "MluI: 2 sites") 1 5 "ACGCGT" "TGCGCA") 
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COMMENT                               (CParagraph 104596584 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MlyI: 9 sites") 10 10 
COMMENT                             "GAGTCNNNNNN" "CTCAGNNNNNN") 
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COMMENT                                             " N1: 3795 (on complementary strand)" 
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COMMENT                                             " N2: 5664 (on complementary strand)" 
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COMMENT                                             " N3: 5848 (on complementary strand)" 
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COMMENT                             "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
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COMMENT                               (CParagraph 104596616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "MnlI: 55 sites") 11 10 
COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
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COMMENT                                       #580=(CLinePar 
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COMMENT                              "MscI: 5 sites") 3 3 "TGGCCA" "ACCGGT") 
COMMENT                       #581=(CRSFolderPar 
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COMMENT                               (CObjectList)) "MseI: 26 sites") 1 3 "TTAA" 
COMMENT                             "AATT") 
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COMMENT                              "NaeI: 1 site") 3 3 "GCCGGC" "CGGCCG") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..737
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xcore2_eHGT_779m
     misc_feature    744..796
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     source          <803..>2810
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             816..833
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /vntifkey="4"
                     /label=6xHis
     misc_feature    2839..3084
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    3098..3301
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    3324..3464
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      3556..3862
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    4381..5238
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      5389..6056
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    813..2810
                     /vntifkey="21"
                     /label=RiboL1-jGCaMP8s
BASE COUNT     1482 a      1524 c      1681 g      1374 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaagtt tgagacaaat taaggaaaag 
      181 cgggttggga ggagtctgag gctgtgggtg gctgcctcga gaggctgctc acaacctgac 
      241 atgtttcaga tcttttgaca cggtcatgca agcaacagaa acttatatgg tttttagtct 
      301 cccaggtagc agggaaatga tgatgtggga ggattagggg ggggaaaata agtttgagac 
      361 aaattaagga aaagcgggtt gggaggagtc tgaggctgtg ggtggctgcc tcgagaggct 
      421 gctcacaacc tgacatgttt cagatctttt gacacggtca tgcaagcaac agaaacttat 
      481 atggttttta gtctcccagg tagcagggaa atgatgatgt gggaggatta ggggggggaa 
      541 aataagtttg agacaaatta aggaaaagcg ggttgggagg agtctgaggc tgtgggtggc 
      601 tgcctcgaga ggctgctcac aacctgacat gtttcagatc ttttgacacg gtcatgcaag 
      661 caacagaaac ttatatggtt tttagtctcc caggtagcag ggaaatgatg atgtgggagg 
      721 attagggggg ggaaaatgag ctcgggctgg gcataaaagt cagggcagag ccatctattg 
      781 cttacatttg cttctgggat ccacccgcca ccatgcatca tcaccatcat cacacgcgtc 
      841 gcaagaagac cttcaaggag gtggccaccg ccgtgaagat catcgccatg ctgatgggcc 
      901 tgaagatcaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttcc 
      961 acatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca 
     1021 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtggagtcca 
     1081 aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg 
     1141 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg 
     1201 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 
     1261 acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca 
     1321 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 
     1381 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 
     1441 acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca 
     1501 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 
     1561 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 
     1621 tggagtacaa cctgccggac caactgactg aagagcagat cgcagaatac aaagaggctt 
     1681 tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg gggacggtga 
     1741 tgcggtctct cggacacaac cccacagaag cagagctgca ggacatgatc aatgaagtag 
     1801 atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg gcaagaaaaa 
     1861 tgaaatacag ggacacggaa gaagaaatta gagaagcgtt cggtgtgttt gataaggatg 
     1921 gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt ggagagaagt 
     1981 taacagatga agaggttgat gaaatgatca gggaagcaga catcgatggg gatggtcagg 
     2041 taaactacga agagtttgta caaatgatga cagcgaaggg gggcagcggt gggtctggag 
     2101 ggactggggg tagcggtggt agcggtggga cagggggaag cggcggtagt ggtggaacaa 
     2161 gcagcaaagt ctcacgcgac accctgtacg aggcggtgcg ggaagtcctg cacgggaacc 
     2221 agcgcaagcg ccgcaagttt ctggagacgg tggagctgca gatcagcctg aagaactacg 
     2281 accctcagaa ggacaaacgt ttctcgggca ccgtcaggct caagtccacc ccacgcccca 
     2341 agttctcggt gtgcgttctg ggggaccagc agcactgtga tgaagccaag gccgtggata 
     2401 tcccccacat ggacatcgag gcgctcaaga aacttaacaa aaacaagaag ttggtcaaga 
     2461 agctggctaa gaagtacgat gcctttttgg cctctgagtc tctgattaag cagatcccac 
     2521 gtatcctggg cccaggccta aacaaggctg gcaagttccc ctccctgctg acacacaatg 
     2581 aaaacatggt ggccaaagtg gatgaggtga aatcgacaat caagttccag atgaagaagg 
     2641 tgctgtgttt ggccgtcgct gttggccacg tgaagatgac cgatgatgag ctagtctaca 
     2701 acattcatct ggctgtcaat ttcttggtgt ccttgcttaa gaaaaactgg caaaacgtgc 
     2761 gggctctgta catcaagagc accatgggca agccccagcg tctgtattag ggcgcgccgc 
     2821 ggccgcgaat tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg 
     2881 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 
     2941 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag 
     3001 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     3061 tgttgggcac tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag 
     3121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 
     3181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 
     3241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 
     3301 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 
     3361 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 
     3421 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 
     3481 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 
     3541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     3601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     3661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     3721 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 
     3781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     3841 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa 
     3901 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     3961 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 
     4021 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 
     4081 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 
     4141 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 
     4201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 
     4261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     4321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     4381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     4441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     4501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 
     4561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     4621 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     4681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     4741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     4801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     4861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     4921 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     4981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     5041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     5101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     5161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     5221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     5281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     5341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     5401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     5461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     5521 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 
     5581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     5641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     5701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     5761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     5821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     5881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     5941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     6001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     6061 t 
//
