LOCUS       CN4948_AAV-3xcore2_eHGT_441h_minBglobin-DIO-SYFP2-WPRE3-BGHpA        5068 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...f6v-azzxtw5
VERSION     urn.local...f6v-azzxtw5
SOURCE      
  ORGANISM  .
COMMENT     
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN4948_AAV-3xcore2_eHGT_441h_minBglobin-DIO-SYFP2-WPRE3-BGHpA|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "CN4948_AAV-3xcore2_eHGT_441h_minBglobin-DIO-SYFP2-WPRE3-BGHpA" 0 
COMMENT       5068 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952538996 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 5 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 7 9 61 102 10 11 12 
COMMENT         13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 103 104 274 
COMMENT         25 26 55 91 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 105 57 38 
COMMENT         36 39 40 41 42 43 95 44 59 99 96 97 45 100 46) (CWordArray) 
COMMENT        (CStringList "AarI" "AatI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" 
COMMENT         "AclI" "AcuI" "AfeI" "AflII" "AgeI" "AluI" "AlwI" "ApaI" "ApaLI" "AscI" 
COMMENT         "AseI" "AsiSI" "AvaI" "AvaII" "AvrII" "BamHI" "BbeI" "Bbr7I" "BbsI" 
COMMENT         "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BciVI" "BclI" "BfaI" "BfrBI" 
COMMENT         "BglII" "BlpI" "BmgBI" "BmrI" "BmtI" "BpmI" "BpuEI" "BsaHI" "BsaI" 
COMMENT         "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiWI" "BsmAI" "BsmBI" "BsmFI" 
COMMENT         "BsmI" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrGI" "BsrI" 
COMMENT         "BssHII" "BssSI" "BstBI" "BstEII" "BstF5I" "BstKTI" "BstNI" "BstUI" 
COMMENT         "BstZ17I" "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "ClaI" "Csp6I" "CviAII" 
COMMENT         "CviJI" "DdeI" "DpnI" "DraI" "EagI" "EarI" "EciI" "Eco57MI" "EcoHI" 
COMMENT         "EcoICRI" "EcoO109I" "EcoRI" "EcoRV" "EsaBC3I" "FatI" "FauI" "FmuI" 
COMMENT         "Fnu4HI" "FokI" "FseI" "FspI" "GdiII" "HaeIII" "HgaI" "HhaI" "HindIII" 
COMMENT         "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" "Hpy99I" "HpyAV" "HpyCH4I" 
COMMENT         "HpyCH4IV" "HpyCH4V" "KasI" "KpnI" "MaeIII" "MboI" "MboII" "MfeI" 
COMMENT         "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MspA1I" "NaeI" "NarI" "NciI" 
COMMENT         "NcoI" "NdeI" "NgoMIV" "NheI" "NlaIII" "NotI" "NruI" "NsiI" "PacI" 
COMMENT         "PciI" "PleI" "PmeI" "PmlI" "Ppu10I" "PsiI" "PspOMI" "PstI" "PvuI" 
COMMENT         "PvuII" "RleAI" "RsaI" "SacI" "SacII" "SalI" "SapI" "Sau96I" "SbfI" 
COMMENT         "ScaI" "SciI" "SelI" "SfaNI" "SfoI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" 
COMMENT         "SphI" "SrfI" "Sse232I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "SwaI" 
COMMENT         "TaiI" "TaqI" "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "UthSI" 
COMMENT         "XbaI" "XhoI" "XmaI" "ZraI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5068 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 3007 0 3504 0 4750 0 0 
COMMENT            "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 41 0 52 0 757 0 2087 0 
COMMENT            2417 0 2428 0 2884 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 226 0 425 0 624 0 813 0 
COMMENT            0 "") #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1829 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1685 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7 0 2470 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 43 0 54 0 2419 0 2430 0 0 
COMMENT            "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 4 41 0 52 0 2417 0 2428 0 0 
COMMENT            "") #10=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AatI" "aggcct" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AatII" "gacgtc" 6 0 1 3257 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 826 0 964 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AceIII" "cagctc" 14 18 14 272 1 279 1 471 1 
COMMENT             478 1 670 1 677 1 990 0 1140 1 1323 0 1644 0 1677 0 2933 1 3130 1 
COMMENT             3872 1 0 "") 
COMMENT        #16=(CRSite (CStringList) "AciI" "ccgc" 2 4 52 38 0 143 1 147 0 180 0 
COMMENT             379 0 578 0 1006 1 1009 1 1162 0 1182 1 1225 1 1320 1 1359 1 1426 1 
COMMENT             1467 1 2014 1 2027 0 2040 0 2315 1 2324 1 2328 0 2433 1 2483 1 2509 
COMMENT             1 2522 0 2559 1 2573 1 2576 1 2604 0 2631 0 3036 0 3062 0 3072 0 
COMMENT             3111 0 3285 1 3332 0 3431 1 3540 1 3617 1 3661 0 3782 1 3828 0 4019 
COMMENT             1 4110 1 4472 0 4481 1 4616 0 4726 1 4847 1 4866 1 4993 1 5021 1 0 
COMMENT             "") 
COMMENT        #17=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 2990 0 3574 0 3947 0 0 
COMMENT             "") 
COMMENT        #18=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 4 1293 1 1536 1 3510 0 
COMMENT             4522 1 0 "") 
COMMENT        #19=(CRSite (CStringList) "AfeI" "agcgct" 4 0 1 1704 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 1696 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AluI" "agct" 3 0 36 12 0 286 0 293 0 315 0 
COMMENT             485 0 492 0 514 0 684 0 691 0 713 0 756 0 882 0 893 0 980 0 1067 0 
COMMENT             1080 0 1154 0 1265 0 1313 0 1529 0 1550 0 1562 0 1634 0 1667 0 2461 
COMMENT             0 2690 0 2947 0 3125 0 3144 0 3823 0 3886 0 3986 0 4507 0 4764 0 
COMMENT             4810 0 4900 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AlwI" "ggatc" 10 11 18 221 1 234 0 420 1 433 
COMMENT             0 619 1 632 0 808 1 821 0 993 1 1192 0 3537 0 3541 1 3858 0 4321 1 
COMMENT             4322 0 4418 1 4420 0 4506 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 956 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AseI" "attaat" 3 0 1 4001 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 4 1028 0 2317 0 3812 0 
COMMENT             4034 0 0 "") #29=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 1 2477 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 4 329 1 528 1 727 1 
COMMENT             2297 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbsI" "gaagac" 9 13 4 334 1 533 1 732 1 2292 
COMMENT             0 0 "") #33=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbvI" "gcagc" 14 18 26 21 0 273 1 280 1 472 1 
COMMENT             479 1 671 1 678 1 880 1 1112 0 1115 0 1141 1 1432 1 1538 0 1911 1 
COMMENT             2029 1 2448 1 2470 0 2607 0 3131 1 3742 1 4133 0 4436 1 4642 1 4645 
COMMENT             1 4735 0 5067 1 0 "") 
COMMENT        #35=(CRSite (CStringList) "BccI" "ccatc" 10 11 15 133 0 341 0 540 0 739 
COMMENT             0 795 0 1116 1 1386 1 1638 1 2131 0 2339 1 2762 1 2794 0 3719 1 
COMMENT             4006 1 4130 1 0 "") 
COMMENT        #36=(CRSite (CStringList) "BceAI" "acggc" 18 20 13 945 1 1098 1 1149 1 
COMMENT             1191 1 1269 1 1359 1 1470 1 1551 1 1599 1 1611 1 2027 0 2750 0 4564 
COMMENT             1 0 "") 
COMMENT        #37=(CRSite (CStringList) "BcefI" "acggc" 18 19 13 946 1 1099 1 1150 1 
COMMENT             1192 1 1270 1 1360 1 1471 1 1552 1 1600 1 1612 1 2027 0 2750 0 4565 
COMMENT             1 0 "") 
COMMENT        #38=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 5 844 1 1737 0 1912 1 
COMMENT             3339 0 4866 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BclI" "tgatca" 2 0 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BfaI" "ctag" 2 0 9 131 0 820 0 1706 0 2112 0 
COMMENT             2340 0 2624 0 3983 0 4318 0 4571 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BglII" "agatct" 2 0 2 2093 0 2304 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 4 311 0 510 0 709 0 1063 
COMMENT             0 0 "") #44=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BmrI" "actggg" 12 11 1 4136 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 1709 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BpmI" "ctggag" 23 21 3 1005 1 1245 1 4107 0 0 
COMMENT             "") 
COMMENT        #48=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 7 231 0 430 0 629 0 
COMMENT             3572 0 4440 0 4681 1 4979 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 5 57 0 2414 0 2474 0 3254 
COMMENT             0 3636 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 1 4110 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "BscAI" "gcatc" 10 12 13 1196 1 1295 1 1310 1 
COMMENT             1584 0 2218 0 2473 1 2509 0 3025 1 3115 0 3478 1 3723 0 3918 1 4970 
COMMENT             1 0 "") 
COMMENT        #52=(CRSite (CStringList) "BseMII" "ctcag" 16 14 16 23 1 98 0 302 1 501 
COMMENT             1 700 1 743 1 1077 0 1095 0 2213 1 2377 1 2452 0 3028 0 3689 0 4206 
COMMENT             1 4372 1 4781 1 0 "") 
COMMENT        #53=(CRSite (CStringList) "BseRI" "gaggag" 17 15 2 1659 1 1968 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "BseYI" "cccagc" 2 6 2 762 1 4760 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 3 1140 1 1464 1 1561 0 0 
COMMENT             "") #56=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 4 3139 0 3181 1 3334 1 
COMMENT             4110 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 2 3139 0 3181 1 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsmFI" "gggac" 16 20 1 140 1 0 "") 
COMMENT        #60=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 16 24 1 97 0 303 1 502 
COMMENT             1 701 1 744 1 1076 0 1094 0 2214 1 2378 1 2451 0 3027 0 3688 0 4207 
COMMENT             1 4373 1 4782 1 0 "") 
COMMENT        #62=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 831 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "BspHI" "tcatga" 2 0 3 3231 0 3336 0 4344 0 0 
COMMENT             "") #64=(CRSite (CStringList) "BspMI" "acctgc" 11 15 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 3 2324 1 2633 0 3334 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 2 3942 0 4116 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 1 974 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsrI" "actgg" 7 5 11 1078 0 1884 0 2866 0 
COMMENT             3531 0 3701 1 3970 0 4013 0 4131 0 4537 0 4649 1 4662 1 0 "") 
COMMENT        #69=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 5 15 0 100 0 956 0 2369 
COMMENT             0 2454 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BssSI" "cacgag" 2 6 4 1503 0 3200 1 3507 0 
COMMENT             4891 0 0 "") #71=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 6 1283 0 1649 0 3107 1 
COMMENT             3730 0 4017 0 4198 0 0 "") 
COMMENT        #74=(CRSite (CStringList) "BstKTI" "gatc" 4 0 23 229 0 428 0 627 0 816 0 
COMMENT             1001 0 1039 0 1187 0 1547 0 2096 0 2307 0 3496 0 3532 0 3549 0 3807 
COMMENT             0 3853 0 3871 0 4212 0 4317 0 4329 0 4407 0 4415 0 4426 0 4501 0 0 
COMMENT             "") 
COMMENT        #75=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 17 176 0 231 0 375 0 430 0 
COMMENT             574 0 629 0 1275 0 1329 0 1404 0 1494 0 1516 0 1641 0 1995 0 2162 0 
COMMENT             4905 0 4918 0 5039 0 0 "") 
COMMENT        #76=(CRSite (CStringList) "BstUI" "cgcg" 3 0 23 17 0 102 0 153 0 221 0 
COMMENT             420 0 619 0 958 0 1041 0 1359 0 2371 0 2456 0 2549 0 2573 0 2593 0 
COMMENT             2969 0 3080 0 3183 0 3185 0 3285 0 3617 0 4110 0 4440 0 5021 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "BstZ17I" "gtatac" 4 0 0 0 "") 
COMMENT        #78=(CRSite (CStringList) "BthCI" "gcngc" 5 0 46 12 0 15 0 40 0 146 0 
COMMENT             149 0 182 0 289 0 296 0 381 0 488 0 495 0 580 0 687 0 694 0 896 0 
COMMENT             1009 0 1103 0 1106 0 1157 0 1164 0 1448 0 1529 0 1927 0 2029 0 2045 
COMMENT             0 2327 0 2330 0 2436 0 2461 0 2464 0 2562 0 2576 0 2598 0 3038 0 
COMMENT             3147 0 3434 0 3663 0 3758 0 3785 0 4124 0 4452 0 4658 0 4661 0 4726 
COMMENT             0 4869 0 5024 0 0 "") 
COMMENT        #79=(CRSite (CStringList) "BtsI" "gcagtg" 9 7 3 1476 1 3757 0 3777 1 0 
COMMENT             "") 
COMMENT        #80=(CRSite (CStringList) "Cac8I" "gcnngc" 4 0 38 7 0 17 0 21 0 25 0 94 
COMMENT             0 98 0 102 0 151 0 291 0 490 0 689 0 958 0 1152 0 1159 0 1469 0 
COMMENT             1483 0 1531 0 1564 0 1707 0 2031 0 2040 0 2120 0 2297 0 2371 0 2375 
COMMENT             0 2379 0 2448 0 2452 0 2456 0 2466 0 2617 0 2631 0 2674 0 3050 0 
COMMENT             4063 0 4454 0 5014 0 5051 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "CdiI" "catcg" 5 5 10 1121 1 1172 1 1301 1 
COMMENT             1316 1 1580 0 2025 0 2214 0 2790 0 3523 0 3924 1 0 "") 
COMMENT        #82=(CRSite (CStringList) "ChaI" "gatc" 5 0 23 230 0 429 0 628 0 817 0 
COMMENT             1002 0 1040 0 1188 0 1548 0 2097 0 2308 0 3497 0 3533 0 3550 0 3808 
COMMENT             0 3854 0 3872 0 4213 0 4318 0 4330 0 4408 0 4416 0 4427 0 4502 0 0 
COMMENT             "") 
COMMENT        #83=(CRSite (CStringList) "Csp6I" "gtac" 2 0 5 975 0 1257 0 2545 0 3018 
COMMENT             0 3694 0 0 "") 
COMMENT        #84=(CRSite (CStringList) "CviAII" "catg" 2 0 16 987 0 1032 0 1422 0 
COMMENT             1452 0 1686 0 1945 0 2300 0 3148 0 3232 0 3337 0 3730 0 3766 0 3844 
COMMENT             0 3854 0 4345 0 5065 0 0 "") 
COMMENT        #85=(CRSite (CStringList) "CviJI" "rgcy" 3 0 93 12 0 37 0 51 0 82 0 118 
COMMENT             0 146 0 165 0 179 0 286 0 293 0 315 0 364 0 378 0 485 0 492 0 514 0 
COMMENT             563 0 577 0 684 0 691 0 713 0 756 0 762 0 786 0 882 0 893 0 980 0 
COMMENT             1067 0 1080 0 1113 0 1154 0 1198 0 1245 0 1265 0 1313 0 1413 0 1491 
COMMENT             0 1514 0 1529 0 1550 0 1562 0 1614 0 1634 0 1667 0 1966 0 2056 0 
COMMENT             2061 0 2086 0 2122 0 2327 0 2355 0 2391 0 2422 0 2436 0 2461 0 2676 
COMMENT             0 2690 0 2704 0 2785 0 2889 0 2927 0 2947 0 3048 0 3052 0 3092 0 
COMMENT             3125 0 3144 0 3198 0 3785 0 3823 0 3886 0 3896 0 3986 0 4052 0 4061 
COMMENT             0 4065 0 4091 0 4132 0 4144 0 4507 0 4536 0 4579 0 4590 0 4655 0 
COMMENT             4734 0 4759 0 4764 0 4810 0 4900 0 4998 0 5024 0 5042 0 5053 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "DdeI" "ctnag" 2 0 17 32 0 84 0 311 0 510 0 
COMMENT             709 0 752 0 1063 0 1081 0 2222 0 2386 0 2438 0 3014 0 3249 0 3675 0 
COMMENT             4215 0 4381 0 4790 0 0 "") 
COMMENT        #87=(CRSite (CStringList) "DpnI" "gatc" 3 0 23 228 0 427 0 626 0 815 0 
COMMENT             1000 0 1038 0 1186 0 1546 0 2095 0 2306 0 3495 0 3531 0 3548 0 3806 
COMMENT             0 3852 0 3870 0 4211 0 4316 0 4328 0 4406 0 4414 0 4425 0 4500 0 0 
COMMENT             "") 
COMMENT        #88=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 3598 0 4290 0 4309 0 0 
COMMENT             "") 
COMMENT        #89=(CRSite (CStringList) "EagI" "cggccg" 2 0 2 144 0 2325 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "EarI" "ctcttc" 8 11 1 3377 1 0 "") 
COMMENT        #91=(CRSite (CStringList) "EciI" "ggcgga" 18 16 6 1197 0 1441 0 2029 0 
COMMENT             4034 0 4862 0 5008 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "Eco57MI" "ctgrag" 23 21 7 1005 1 1245 1 1293 
COMMENT             1 1536 1 3510 0 4107 0 4522 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "EcoHI" "ccsgg" 1 0 17 40 0 41 0 51 0 52 0 77 
COMMENT             0 1001 0 1655 0 2391 0 2416 0 2417 0 2427 0 2428 0 3102 0 3137 0 
COMMENT             3638 0 3989 0 4685 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 1 756 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "EcoO109I" "rggnccy" 3 0 1 3196 0 0 "") 
COMMENT        #96=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1837 0 0 "") 
COMMENT        #97=(CRSite (CStringList) "EsaBC3I" "tcga" 3 0 13 827 0 965 0 1172 0 
COMMENT             1301 0 1316 0 1343 0 1637 0 1834 0 2089 0 2100 0 2743 0 3523 0 4967 
COMMENT             0 0 "") 
COMMENT        #98=(CRSite (CStringList) "FatI" "catg" 1 0 16 986 0 1031 0 1421 0 1451 
COMMENT             0 1685 0 1944 0 2299 0 3147 0 3231 0 3336 0 3729 0 3765 0 3843 0 
COMMENT             3853 0 4344 0 5064 0 0 "") 
COMMENT        #99=(CRSite (CStringList) "FauI" "cccgc" 10 12 7 1352 1 1460 1 2047 0 
COMMENT             2569 1 2638 0 3069 0 3079 0 0 "") 
COMMENT        #100=(CRSite (CStringList) "FmuI" "ggncc" 5 0 10 1031 0 1114 0 1515 0 
COMMENT              2320 0 2786 0 3199 0 3815 0 4037 0 4054 0 4133 0 0 "") 
COMMENT        #101=(CRSite (CStringList) "Fnu4HI" "gcngc" 3 0 46 10 0 13 0 38 0 144 0 
COMMENT              147 0 180 0 287 0 294 0 379 0 486 0 493 0 578 0 685 0 692 0 894 0 
COMMENT              1007 0 1101 0 1104 0 1155 0 1162 0 1446 0 1527 0 1925 0 2027 0 
COMMENT              2043 0 2325 0 2328 0 2434 0 2459 0 2462 0 2560 0 2574 0 2596 0 
COMMENT              3036 0 3145 0 3432 0 3661 0 3756 0 3783 0 4122 0 4450 0 4656 0 
COMMENT              4659 0 4724 0 4867 0 5022 0 0 "") 
COMMENT        #102=(CRSite (CStringList) "FokI" "ggatg" 15 19 6 1290 0 1656 0 3094 1 
COMMENT              3737 0 4024 0 4205 0 0 "") 
COMMENT        #103=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 0 0 "") 
COMMENT        #104=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 3953 0 0 "") 
COMMENT        #105=(CRSite (CStringList) "GdiII" "cggccr" 2 6 9 144 1 144 0 177 1 376 
COMMENT              1 575 1 1612 1 2325 0 2325 1 3783 0 0 "") 
COMMENT        #106=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 25 37 0 82 0 118 0 146 0 
COMMENT              179 0 378 0 577 0 1113 0 1198 0 1514 0 1614 0 2327 0 2355 0 2391 0 
COMMENT              2436 0 2785 0 2927 0 3198 0 3785 0 4052 0 4132 0 4590 0 5024 0 
COMMENT              5042 0 5053 0 0 "") 
COMMENT        #107=(CRSite (CStringList) "HgaI" "gacgc" 11 16 7 46 1 142 1 2422 0 3086 
COMMENT              0 3644 0 4374 1 4952 1 0 "") 
COMMENT        #108=(CRSite (CStringList) "HhaI" "gcgc" 4 0 32 17 0 19 0 102 0 104 0 
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COMMENT        #186=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 819 0 0 "") 
COMMENT        #187=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2087 0 0 "") 
COMMENT        #188=(CRSite (CStringList) "ZraI" "gacgtc" 4 0 1 3255 0 0 "")) 
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COMMENT        #198=(CFSignal (CObList) "BGH-pA" 21 0 0 2099 2302 0 (CStringList) 
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COMMENT               "width 5 offset 0\"") 1 1 1 1 "2331..2471") 
COMMENT        #200=(CFSignal (CObList) "f1 Origin" 33 0 0 2563 2869 0 (CStringList) 
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COMMENT        #202=(CFSignal (CObList) "pUC ori" 33 0 0 4396 5063 0 (CStringList) 
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COMMENT                             "Gibson/Homology Cloning ligation product." 1) 
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COMMENT                             "Original Accession Number(s): <a href=\"ncbilink:dnarna/urn.local...f6v-azzxtw5\">urn.local...f6v-azzxtw5</a>" 
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COMMENT                              "BsaI: 1 site") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                              "BsmAI: 4 sites") 6 10 "GTCTCNNNNNN" "CAGAGNNNNNN") 
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FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    156..752
                     /created_by="ximena.opitzaraya"
                     /vntifkey="21"
                     /label=3xcore2_eHGT_441h
     misc_feature    759..811
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    836..869
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    920..953
                     /vntifkey="21"
                     /label=loxP
     misc_feature    954..1703
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    complement(1710..1743)
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    complement(1794..1827)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1840..2085
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    2099..2302
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    2331..2471
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2563..2869
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3388..4245
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4396..5063
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT     1136 a      1326 c      1292 g      1314 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtcccac acagccccca ccacctggcc 
      181 gcccacctga aaaacttttc cccaagttct tgagaagtcg cgtaggatcc aggagagaaa 
      241 aatcaaagta ataaaacctc atttaattcc taacggaggg cagagctgcg agctgcaatt 
      301 atcttaatgc tgagctattt ttactctctc tccatctctg tcttctgcct ttctcccaca 
      361 cagcccccac cacctggccg cccacctgaa aaacttttcc ccaagttctt gagaagtcgc 
      421 gtaggatcca ggagagaaaa atcaaagtaa taaaacctca tttaattcct aacggagggc 
      481 agagctgcga gctgcaatta tcttaatgct gagctatttt tactctctct ccatctctgt 
      541 cttctgcctt tctcccacac agcccccacc acctggccgc ccacctgaaa aacttttccc 
      601 caagttcttg agaagtcgcg taggatccag gagagaaaaa tcaaagtaat aaaacctcat 
      661 ttaattccta acggagggca gagctgcgag ctgcaattat cttaatgctg agctattttt 
      721 actctctctc catctctgtc ttctgccttt ctgagctcgg gctgggcata aaagtcaggg 
      781 cagagccatc tattgcttac atttgcttct gggatcctct agagtcgact ccggaataac 
      841 ttcgtatagg atactttata cgaagttatg cagaatggta gctggattgt agctgctatt 
      901 agcaatatga aacctcttaa taacttcgta tagcatacat tatacgaagt tatggcgcgc 
      961 cgtcgactta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact 
     1021 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc 
     1081 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt 
     1141 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct 
     1201 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact 
     1261 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc 
     1321 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt 
     1381 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt 
     1441 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg cacgccgtag cccagggtgg 
     1501 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca 
     1561 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta 
     1621 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc 
     1681 tcaccatggt ggcgaccggt agcgctagca taacttcgta taaagtatcc tatacgaagt 
     1741 tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa agaataactt 
     1801 cgtataatgt atgctatacg aagttatgaa ttcgatatca taatcaacct ctggattaca 
     1861 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     1921 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     1981 ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc 
     2041 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggctcga gagatcttcg 
     2101 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 
     2161 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 
     2221 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 
     2281 tgggaagaca atagcaggca tgagatctca cgtgcggacc gagcggccgc aggaacccct 
     2341 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc 
     2401 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag 
     2461 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 
     2521 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     2581 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     2641 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     2701 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     2761 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     2821 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     2881 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     2941 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 
     3001 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 
     3061 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 
     3121 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 
     3181 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 
     3241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     3301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     3361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     3421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     3481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     3541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     3601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     3661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     3721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     3781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     3841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     3901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     3961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     4021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     4081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     4141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     4201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     4261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     4321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     4381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     4441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     4501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     4561 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     4621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     4681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     4741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     4801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     4861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     4921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     4981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     5041 gccttttgct ggccttttgc tcacatgt 
//
