LOCUS       hSHIP2_pCMV6_ent        8655 bp DNA     circular SYN 04-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Tomas Gregor
  TITLE     Direct Submission
  JOURNAL   Exported Apr 4, 2024 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     975..994
                     /label=SHIP2 F
     CDS             1029..4028
                     /codon_start=SHIP2 with V5-HIS tag
                     /label=SHIP2 with V5-HIS tag
                     /translation="MASACGAPGPGGALGSQAPSWYHRDLSRAAAEELLARAGRDGSF
                     LVRDSESVAGAFALCVLYQKHVHTYRILPDGEDFLAVQTSQGVPVRRFQTLGELIGLY
                     AQPNQGLVCALLLPVEGEREMKNKHSKQDEPDMISVFIGTWNMGSVPPPKNVTSWFTS
                     KGLGKTLDEVTVTIPHDIYVFGTQENSVGDREWLDLLRGGLKELTDLDYRPIAMQSLW
                     NIKVAVLVKPEHENRISHVSTSSVKTGIANTLGNKGAVGVSFMFNGTSFGFVNCHLTS
                     GNEKTARRNQNYLDILRLLSLGDRQLNAFDISLRFTHLFWFGDLNYRLDMDIQEILNY
                     ISRKEFEPLLRVDQLNLEREKHKVFLRFSEEEISFPPTYRYERGSRDTYAWHKQKPTG
                     VRTNVPSWCDRILWKSYPETHIICNSYGCTDDIVTSDHSPVFGTFEVGVTSQFISKKG
                     LSKTSDQAYIEFESIEAIVKTASRTKFFIEFYSTCLEEYKKSFENDAQSSDNINFLKV
                     QWSSRQLPTLKPILADIEYLQDQHLLLTVKSMDGYESYGECVVALKSMIGSTAQQFLT
                     FLSHRGEETGNIRGSMKVRVPTERLGTRERLYEWISIDKDEAGAKSKAPSVSRGSQEP
                     RSGSRKPAFTEASCPLSRLFEEPEKPPPTGRPPAPPRAAPREEPLTPRLKPEGAPEPE
                     GVAAPPPKNSFNNPAYYVLEGVPHQLLPPEPPSPARAPVPSATKNKVAITVPAPQLGH
                     HRHPRVGEGSSSDEESGGTLPPPDFPPPPLPDSAIFLPPSLDPLPGPVVRGRGGAEAR
                     GPPPPKAHPRPPLPPGPSPASTFLGEVASGDDRSCSVLQMAKTLSEVDYAPAGPARSA
                     LLPGPLELQPPRGLPSDYGRPLSFPPPRIRESIQEDLAEEAPCLQGGRASGLGEAGMS
                     AWLRAIGLERYEEGLVHNGWDDLEFLSDITEEDLEEAGVQDPAHKRLLLDTLQLSKTR
                     TRPLEGKPIPNPLLGLDSTRTGHHHHHH"
     misc_feature    1089..1379
                     /label=SH2 PDB
     CDS             complement(2101..2112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3939..3956
                     /label=linker to Myc
     CDS             3960..4001
                     /codon_start=1
                     /product="V5 tag"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4057..4073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4084..4706
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     rep_origin      complement(4855..5443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    5772..5819
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal"
     CDS             complement(6051..6845)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and
                     G418  (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6880..7237)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6894..7029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7239..7343)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(7370..7800,1..25)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
BASE COUNT     1722 a   2225 c   2124 g   1729 t
ORIGIN      
          1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
         61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
        121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
        181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
        241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
        301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
        361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
        421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
        481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
        541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
        601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
        661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
        721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
        781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
        841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
        901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
        961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
       1021 cgatcgccat ggcctcggcc tgcggggcgc cgggcccggg gggcgccctg ggcagccagg
       1081 ccccctcctg gtaccaccgc gacctgagcc gggcggccgc ggaggagctg ctggcccggg
       1141 cgggccgcga tggcagcttc ctggtccgag acagcgagag cgtggcgggg gccttcgcgc
       1201 tctgcgtcct gtatcagaag catgtgcaca cgtatcgcat tctgcctgat ggagaagatt
       1261 tcttggctgt gcagacctcg cagggtgtgc ctgtgcgccg cttccagacc ctgggtgagc
       1321 tcatcggcct gtacgcccag cccaaccagg gccttgtgtg cgccctgctt cttcctgtag
       1381 agggtgagcg agagatgaag aacaagcact ccaagcagga cgagcccgac atgatctcag
       1441 tcttcatagg cacctggaac atgggaagtg taccacctcc aaaaaacgtg acatcctggt
       1501 tcacatcgaa gggtctgggg aagaccctgg acgaggtcac agtgaccata ccccatgaca
       1561 tctatgtctt tgggacccag gagaactcag tgggcgaccg cgagtggctg gacctactgc
       1621 gcgggggcct caaggagctt acggatctgg attaccgccc gattgccatg caatcactgt
       1681 ggaatatcaa ggtggcagtg ctggtcaagc cagagcacga gaaccgtatc agccatgtca
       1741 gtacgtccag tgtgaagact ggcatcgcca acaccctggg gaacaagggg gctgtgggcg
       1801 tctccttcat gtttaatggc acctcatttg gctttgtgaa ttgtcacctc acctcgggaa
       1861 atgagaagac ggctcggagg aaccaaaact acttggacat cctgcggctg ctctcgctgg
       1921 gcgaccggca gctcaatgcc tttgacatct ctctgcgttt cacacacctc ttctggtttg
       1981 gggacctcaa ctaccgcctg gacatggata tccaggagat cctgaactac atcagcagga
       2041 aagagtttga gcccctcctc agggtggacc agctcaacct ggagcgggag aagcacaagg
       2101 tcttccttcg attcagtgag gaggagatct ccttcccacc cacctaccgc tatgagcggg
       2161 gttcccggga cacatatgcc tggcacaagc agaagccaac tggggtccgg accaatgtgc
       2221 cctcatggtg tgaccggatt ctgtggaaat cctaccctga aactcacatc atctgcaatt
       2281 cttatggttg cactgatgac atcgtcacca gcgaccattc ccccgtgttt gggacatttg
       2341 aggttggagt tacctcccag ttcatctcca agaaagggct ctcaaagact tcagaccagg
       2401 cctacattga gtttgagagc atcgaggcca ttgtgaagac agccagccgc accaagttct
       2461 tcatcgagtt ctactctacc tgcctggagg aatacaagaa gagctttgag aatgatgccc
       2521 agagcagtga caacatcaac ttcctcaaag tgcagtggtc ttcacgccag ctgcccacgc
       2581 tcaaaccaat tctggctgat atcgagtacc tgcaggacca gcacctcctg ctcacagtca
       2641 agtccatgga tggctatgaa tcctatgggg agtgtgtggt tgcactcaaa tccatgatcg
       2701 gcagcacggc ccaacagttc ctgaccttcc tatcccaccg tggcgaggag acaggcaata
       2761 tcagaggctc catgaaggtg cgggtgccca cggagcgcct gggcacccgt gagcggctct
       2821 acgagtggat cagcattgat aaggatgagg caggagcaaa gagcaaagcc ccctctgtgt
       2881 cccgagggag ccaggagccc aggtcaggga gccgcaagcc agccttcaca gaggcctcct
       2941 gcccgctctc caggttattt gaagaaccag agaaaccgcc accaacgggg aggcccccag
       3001 ccccaccccg agcagctccc cgggaggagc ccttgacccc caggttgaag ccagagggag
       3061 ctcctgaacc agaaggggtg gcggcccccc cacccaagaa cagcttcaat aaccctgcct
       3121 actacgtcct tgaaggggtc ccgcaccagc tgctgccccc ggagccaccc tcgcctgcca
       3181 gggcccctgt cccatctgcc accaagaaca aagtggccat tacagtgcct gctccacagc
       3241 ttgggcacca ccggcaccct cgtgtgggag aggggagttc ttcagatgag gagtctggag
       3301 gcacactgcc ccctccagac tttccacctc caccactgcc ggactcagcc atcttcctgc
       3361 cccccagcct ggatccttta ccagggccag tggtccgggg ccgtggtggg gctgaggccc
       3421 gtggcccacc acctcccaag gcccatccaa ggcctccact gcccccaggc ccctcaccag
       3481 ccagcacttt cctgggggaa gtggccagtg gggatgaccg gtcctgctcg gtgctgcaga
       3541 tggccaagac gctgagcgag gtggactatg cccctgctgg gcctgcacgc tcagcgctcc
       3601 tcccaggccc cctggagctg cagccccccc ggggactgcc ctcggactat ggccggcccc
       3661 tcagcttccc tccaccccgc atccgggaga gcatccagga agacctggca gaggaggctc
       3721 cgtgcctgca gggcgggcgg gccagcgggc tgggcgaggc aggcatgagt gcctggctgc
       3781 gggccatcgg cttggagcgc tatgaggagg gcctggtgca taatggctgg gacgacctgg
       3841 agtttctcag tgacatcacc gaggaggact tggaggaggc tggggtgcag gacccggctc
       3901 acaagcgcct ccttctggac accctgcagc tcagcaagac gcgtacgcgg ccgctcgagG
       3961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCGTACCGGT CATCATCACC
       4021 ATCACCATTG AGCTAGCgtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
       4081 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
       4141 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
       4201 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
       4261 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
       4321 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
       4381 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
       4441 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
       4501 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
       4561 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
       4621 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
       4681 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
       4741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
       4801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
       4861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
       4921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
       4981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
       5041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
       5101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
       5161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
       5221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
       5281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
       5341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
       5401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
       5461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
       5521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
       5581 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
       5641 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
       5701 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
       5761 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
       5821 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
       5881 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
       5941 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
       6001 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
       6061 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
       6121 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
       6181 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
       6241 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
       6301 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
       6361 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
       6421 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
       6481 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
       6541 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
       6601 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
       6661 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
       6721 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
       6781 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
       6841 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
       6901 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
       6961 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
       7021 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
       7081 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
       7141 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
       7201 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
       7261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
       7321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
       7381 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
       7441 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
       7501 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
       7561 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
       7621 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
       7681 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
       7741 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
