LOCUS       Exported               11556 bp ds-DNA     linear   SYN 12-JAN-2024
DEFINITION  V2: TRE-SRRD-linker-mRuby3-2A-neo-WPRE (NO P2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
       61 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     1021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3481 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3541 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3601 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3661 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3721 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3781 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3841 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3901 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3961 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4021 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4081 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4141 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4201 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4261 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     6061 ttggttaatt acagcagaga tccagtttgg ttaatcgagt ttactcccta tcagtgatag
     6121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
     6181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     6241 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
     6301 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
     6361 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
     6421 ggcaccatgg ctgcggccgc agctgcggcg ctggaatcct ggcaggcggc ggctccgcgg
     6481 aagaggcgct ccgcggctcg acggccgcgg cggagggagg cggcgccccg ggggagagag
     6541 gcggcgcccc gggggagaga ggcggcgccc cggggccccg aggcggagtt cgagtctgac
     6601 agcggagtcg tgcttcgtcg catctgggag gctgagaagg acctgtttat ctctgatttc
     6661 tggagttcag cactagaaac catcaataga tgtctcacaa aacatctgga acaactgaag
     6721 gcccctgtgg ggactctttc agacatcttt ggaaacctgc atcttgactc attgccagag
     6781 gagtcagatg tggccactga ttctatccca agagagatct tggtcacagg aacctgccat
     6841 ttgaagtgtg tgtgttacgg cattgggaac tttgccacct gcatcgtagc tagaaaccag
     6901 ctaacgtttt tgctgctttt gttggaaaag tgccagattc ccagaagtca ctgttgggta
     6961 tatgaccctc tgtttagcca acttgaaatt gaagtcctta acacccttgg tgtgactgtt
     7021 ctcagtgaga acgaggaagg gaaacggagt attcgcgggg agcctaccat cttttacatg
     7081 ctccattgtg ggacggcctt gtacaacaat cttttatgga gtaactggtc agtagatgcc
     7141 ctttctaaga tggtcatcat tgggaacagt ttcaaaggac ttgaggagag gttgttggca
     7201 aggattctgc agaaaaatta tccctacatt gcaaagattt taaaaggact ggaggagctt
     7261 gagtttcctc agacttcaca atacatggac atatttaatg atacctctgt ccactggttc
     7321 cctgtgcaaa agctagaaca gctctccata gatatttggg agtttcggga agaaccagat
     7381 tatcaggact gtgaggacct tgaaatcatc aggaacaaga gagaagatcc ttctgctact
     7441 gacggatccg gaagtggctc aagcggagtg tctaagggcg aagagctgat caaggaaaat
     7501 atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt
     7561 gaaggagaag gcagaccgta cgagggagtg caaaccatga ggatcaaagt catcgaggga
     7621 ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtacc
     7681 tttatcaagt acccggccga catccctgat ttctttaaac agtcctttcc tgagggtttt
     7741 acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc
     7801 agccttgagg atggcgagct cgtctacaac gtcaaggtca gaggggtaaa ctttccctcc
     7861 aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat
     7921 ccagcagatg gtggtctgag aggatacact gacatcgcac tgaaagttga tggtggtggc
     7981 catctgcact gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg gaacatcaag
     8041 atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tcgaggagag tgacaatgaa
     8101 acctacgtag tgcaaagaga agtggcagtt gccaaataca gcaaccttgg tggtggcatg
     8161 gacgagctgt acaagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     8221 gtcgaagaga atcctggacc gggatcggcc attgaacaag atggattgca cgcaggttct
     8281 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     8341 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     8401 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     8461 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     8521 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     8581 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     8641 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     8701 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     8761 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     8821 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     8881 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     8941 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     9001 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagaattcgc tagctaggtc
     9061 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     9121 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
     9181 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     9241 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     9301 agaacacagg accggttcta gagcgctgcc accatgtcta gactggacaa gagcaaagtc
     9361 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     9421 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     9481 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     9541 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     9601 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     9661 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     9721 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     9781 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     9841 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     9901 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     9961 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
    10021 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
    10081 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
    10141 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    10201 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    10261 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    10321 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    10381 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    10441 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
    10501 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    10561 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    10621 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
    10681 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    10741 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
    10801 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10861 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10921 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10981 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtaaacccgc tgatcagcct
    11041 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    11101 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    11161 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    11221 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
    11281 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
    11341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    11401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    11461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    11521 aacttgatta gggtgatggt tcacgtagtg ggccat
//
